• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.11
• /
• pp.6273-6280
• /
• 2013

Goniothalamin, a natural compound extracted from Goniothalamus sp. belonging to the Annonacae family, possesses anticancer properties towards several tumor cell lines. This study focused on apoptosis induction by goniothalamin (GTN) in the Hela cervical cancer cell line. Cell growth inhibition was measured by MTT assay and the $IC_{50}$ value of goniothalamin was $3.2{\pm}0.72{\mu}g/ml$. Morphological changes and biochemical processes associated with apoptosis were evident on phase contrast microscopy and fluorescence microscopy. DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. It could be concluded that goniothalamin show a promising cytotoxicity effect against cervical cancer cells (Hela) and the cell death mode induced by goniothalamin was apoptosis.

• Advances in Traditional Medicine
• /
• v.7 no.4
• /
• pp.395-408
• /
• 2007

This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid, ${\beta}$-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.

• Biomolecules & Therapeutics
• /
• v.22 no.5
• /
• pp.426-430
• /
• 2014

Prostate cancer is the most frequently diagnosed cancer. Although prostate tumors respond to androgen ablation therapy at an early stage, they often acquire the potential of androgen-independent growth. Elevated transcriptional activity of androgen receptor (AR) and/or signal transducer and activator of transcription-3 (STAT3) contributes to the proliferation of prostate cancer cells. In the present study, we examined the effect of resveratrol, a phytoalexin present in grapes, on the reporter gene activity of AR and STAT3 in human prostate cancer (LNCaP-FGC) cells stimulated with interleukin-6 (IL-6) and/or dihydrotestosterone (DHT). Our study revealed that resveratrol suppressed the growth of LNCaP-FGC cells in a time- and concentration-dependent manner. Whereas the AR transcriptional activity was induced by treatment with either IL-6 or DHT, the STAT3 transcriptional activity was induced only by treatment with IL-6 but not with DHT. Resveratrol significantly attenuated IL-6-induced STAT3 transcriptional activity, and DHT- or IL-6-induced AR transcriptional activity. Treatment of cells with DHT plus IL-6 significantly increased the AR transcriptional activity as compared to DHT or IL-6 treatment alone and resveratrol markedly diminished DHT plus IL-6-induced AR transcriptional activity. Furthermore, the production of prostate-specific antigen (PSA) was decreased by resveratrol in the DHT-, IL-6- or DHT plus IL-6-treated LNCaP-FGC cells. Taken together, the inhibitory effects of resveratrol on IL-6- and/or DHT-induced AR transcriptional activity in LNCaP prostate cancer cells are partly mediated through the suppression of STAT3 reporter gene activity, suggesting that resveratrol may be a promising therapeutic choice for the treatment of prostate cancer.

• Korean Journal of Veterinary Research
• /
• v.34 no.4
• /
• pp.769-779
• /
• 1994

The root of Bupleurum falcatum L.(BF) has been widely used in oriental medicine as a major camponent in many prescriptions for chronic hepatitis, renal disease, tuberculosis and some other infectious diseases. Many attempts have done to investigate the therapeutic effects of these principles. However, any kinds of screenig on immune regulatory- and antitumor- effects of BF has not been reported. The present study, therefore, was undertaken to investigate the BF-effects on cellular- and humoral-immune responses, phagocytic activities of macrophages, lymphokine- and Immunoglobulin(Ig)-production of lymphocytes, tumorigenesis of implanted sarcoma 180 cells and B16 melanoma cells, and proliferations of some tumor cell lines(Fsa II, 3LL and EL4). BF increased phagocytic activities of mouse peritoneal macrophages in a dose- and time-dependent fashion. Arthus reaction and antibody responses to SRBC were slightly enhanced but delayed hypersensitivity was depresed when BF was injected before- and after-SRBC sensitization. BF inhibited the proliferative responses of human tonsillar lymphocytes to PHA- and Con A-stimulation but slightly augmented the response of these cells to Staphylococcus aureus Cowan 1(SAC)-activation. Ig secretion of human mononuclear cells activated with SAC was slightly increased by BF. BF significantly augmented the SAC-induced IL 6 production of human mononuclear cells but not influenced Con Ainduced IL 2 secretion. NK cell activities of mouse splenocytes were somewhat increased when BF was pretreated and this responses were due to the increment of binding affinities of effector cells to target cells and of lytic activities of effector cells against target cells. In vitro BF significantly inhibited the proliferations of cancer cells such as Fsa II, 3LL and EL4 strains. BF decreased not only the frequency of tumor induction but also the tumor size per sarcoma 180 or B16 cell-implanted mouse. Taken together, these results indicate that BF is one of the potential immunomodulator, and suggest its possibility to be used as a desirable antitumor agent.

• The Journal of Korean Society for School & Community Health Education
• /
• v.21 no.3
• /
• pp.1-19
• /
• 2020

Objectives: This study was attempted to examine the relationship between hand grip strength and dyslipidemia. and it was analyze the effect of hand grip strength on dyslipidemia and discuss the problems that may occur. Methods: The study focused on 'the National Health and Nutrition Survey's 7th Year 1, 2, and 3 (2016-2018)' as their primary data. Among people who measured dyslipidemia and relative hand grip strength, 12,636 people were selected as the final study after excluding missing values. Frequency analysis, mean analysis, and regression analysis was used to determine dyslipidemia and hand grip strength indices according to the subjects' characteristics study and verify the research hypothesis. Results: Outcomes show that 4,542 (35.9%) of the subjects were dyslipidemia, and when the hand grip strength of the subjects increased by 1kg, Dyslipidemia decreased by 0.217 less. The result of subdivided and analyzed dependent variables presented that every time the hand grip strength increased by 1kg, the L-HDL -C decreased by 0.325 less, but it did not affect H-LDL-C and H-TG. Conclusion: In order to reduce dyslipidemia, it is essential to recognize the necessity of muscular exercise and fulfill it. Focus on health care, such as health conditions, health behavior, and make efforts to participate in health education. If so, it is expected to help reduce the economic burden, such as the treatment of dyslipidemia and improve the quality of life free from mechanical circulatory diseases.

• Food Science of Animal Resources
• /
• v.37 no.6
• /
• pp.917-925
• /
• 2017

Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.

• International Journal of Concrete Structures and Materials
• /
• v.5 no.1
• /
• pp.65-73
• /
• 2011

Corrosion of steel bars embedded in concrete admixed with 0%, 2% and 4% calcium nitrite (CN), having compressive strengths of 20 and 46 MPa was investigated. Reinforced concrete specimens were immersed in 3% NaCl solutions for 1, 7 and 15 days where 0.4A external current was applied to accelerate the chemical reactions. Corrosion rate was measured by retrieving electrochemical data via potentiodynamic polarization technique. Pull-out tests of reinforced concrete specimens were then conducted to assess the corroded steel-concrete bond characteristics. Experimental results showed that corrosion rate of steel bars and steel-concrete bond strength were dependent on concrete strength, amount of CN added and accelerated corrosion period. As concrete strength increased from 20 to 46 MPa, corrosion rate of embedded steel decreased. The addition of 2% CN to concrete of 20 MPa was not effective in retarding corrosion of steel at long time of exposure. However, the combination of higher strength concrete and 2% or 4% CN appear to be a desirable approach to reduce the effect of chloride-induced corrosion of steel reinforcement. After 1 day of corrosion acceleration, specimens without CN showed higher bond strength in both concrete mixes than those with CN. After 7 and 15 days of exposure, the higher concentration of CN, the higher bond strength in both concrete mixes achieved, except for the concrete specimen of 20 MPa compressive strength with 2% CN that recorded the highest deterioration in bond strength at 15 days of exposure.

• Journal of the Korean Institute of Gas
• /
• v.17 no.2
• /
• pp.70-77
• /
• 2013

The present study has been carried out to analyze the effect of steam hammering on the steam piping system including the final superheater, the high pressure turbine, check valve and the first reheater by sudden stoping of main stop valve in a power plant. For the present steam hammering analysis, the well known Flowmaster software has been used to model the steam piping system and the time dependent characteristics of pressure and steam mass flow rate has been conducted. Using the result of the unsteady pressure and steam mass flow rate, the forces acting on the elbows in the piping system has been derived. From the present analysis, it has been elucidated that the elbow just before the main stop valve and the elbow near the connection pipe between bypass pipe and check valve had the largest force among the elbows in the steam piping system. The structural safety diagnostics study on the elbow and the supporting structures of the steam piping system of a power plant will be conducted in the future by the present results of the forces acting on the elbow.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.21 no.4
• /
• pp.88-95
• /
• 2007

Korea is highly dependent on foreign countries for energy while at the same time having a high energy-consumption industrial structure. Therefore, logical improvements in energy use efficiency and nationwide energy saving are becoming more and more important in coping with the worldwidehigh oil prices and environmental issues such as listed in the Kyoto Protocol to the United Nations Framework Convention on Climate Change. Consequently, a study was conducted on the average annual load factor in domestic areas to set a reasonable and reliable technology standard plan for high-efficiency transformers. The average annual load factor in Korea was discovered to be 18.4[%] classified by industry. This factor is expected to be used in arranging a domestic standard for a minimum efficiency system for transformers, and in reviewing and supplementing the standard transformers plan for the High Energy-Efficiency Appliance Certification. The expected effect from the establishment of the technology standards plan for highly efficient transformers is the expansion of the manufacturing and distribution of highly efficient transformers that are suitable for domestic use. These will lead to electricity cost savings for users, strengthening the related industries' market competitive powers and the effective reduction of greenhouse gases on a national level by drastically reducing loss from transformers, which accounts for a large portion of the total electric supply losses.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.10
• /
• pp.5849-5854
• /
• 2013

Background: Apoptosis may be induced after Bcl-2 expression is inhibited in proliferative cancer cells. This study focused on the effect of autophagy activation by ABT737 on anti-tumor effects of epirubicin. Methods: Cytotoxic effects of ABT737 on the HepG2 liver cancer cell line were assessed by MTT assay and cell apoptosis through flow cytometry. Mitochondrial membrane potential was measured by fluorescence microscopy. Monodansylcadaverin (MDC) staining was used to detect activation of autophagy. Expression of p53, p62, LC3, and Beclin1, apoptotic or autophagy related proteins, was detected by Western blotting. Results: ABT737 and epirubicin induced growth inhibition in HepG2 cells in a dose- and time-dependent manner. Both ABT737 and epirubicin alone could induce cell apoptosis with a reduction in mitochondrial membrane potential as well as increased apoptotic protein expression. Further increase of apoptosis was detected when HepG2 cells were co-treated with ABT373 and epirubicin. Furthermore, our results demonstrated that ABT373 or epirubicin ccould activate cell autophagy with elevated autophagosome formation, increased expression of autophagy related proteins and LC3 fluorescent puncta. Conclusions: ABT737 influences cancer cells through both apoptotic and autophagic mechanisms, and ABT737 may enhance the effects of epirubicin on HepG2 cells by activating autophagy and inducing apoptosis.