• Nuclear Engineering and Technology
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• v.40 no.1
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• pp.77-86
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• 2008

This paper describes the nonlinear analyses of a 1:4 scale model of a prestressed concrete containment vessel (PCCV) using an axisymmetric model and a three-dimensional model. These two models are refined by comparison of the analysis results and with testing results. This paper is especially focused on the analysis of behavior under pressure and the temperature effects revealed using an axisymmetric model. The temperature-dependent degradation properties of concrete and steel are considered. Both geometric and material nonlinearities, including thermal effects, are also addressed in the analyses. The Menetrey and Willam (1995) concrete constitutive model with non-associated flow potential is adopted for this study. This study includes the results of the predicted thermal and mechanical behaviors of the PCCV subject to high temperature loading and internal pressure at the same time. To find the effect of high temperature accident conditions on the ultimate capacity of the liner plate, reinforcement, prestressing tendon and concrete, two kinds of analyses are performed: one for pressure only and the other for pressure with temperature. The results from the test on pressurization, analysis for pressure only, and analyses considering pressure with temperatures are compared with one another. The analysis results show that the temperature directly affects the behavior of the liner plate, but has little impact on the ultimate pressure capacity of the PCCV.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.342-354
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• 2018

Suaeda japonica is a halophytic plant that lives in mudflat at intertidal zone of western and southern coastal areas of Korea. The seawater-living plants showed a purple color during their whole life. In contrast, freshwater-living plants displayed a green color in leaves. When seawater-living plants were transferred to potting soil, the purple color was gradually changed to green in the leaves. The extracted purple pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. The LC-MS analysis of the extracted pigment compound showed the presence of two major protonated molecular ions ($[M+H]^+$) at m/z 651.1 and m/z 827.1. Antioxidant activity of the pigment compound was determined using stable free radical DPPH assay. It was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 mg/ml. The anticancer activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.

• Structural Engineering and Mechanics
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• v.78 no.1
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• pp.31-39
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• 2021

To investigate numerically the effect of all parameters on the outcome of an aircraft impact into robust engineering structures like nuclear power plant containments is a tedious task. In order to reduce the problem to a manageable size, we propose a single dimensionless parameter, the damage potential, to characterize the main features of the impact. The damage potential, which is the ratio of the initial kinetic energy of the aircraft to the work required to crush it, enables us to find the crucial parameter settings that need to be modelled numerically in detail. We show in this paper that the damage potential is indeed the most important parameter of the impact that determines the time-dependent reaction force when either finite element (FE) modelling or the Riera model is applied. We find that parameters that do not alter the damage potential, like elasticity of the target, are of secondary importance and if parameters are altered in a way that the damage potential remains the same then the course of the impact remains similar. We show, however, that the maximum value of the reaction force can be higher in case of elastic targets than in case of rigid targets due to the vibration of the target. The difference between the Riera and FE model results is also found to depend on the damage potential.

• Steel and Composite Structures
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• v.39 no.2
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• pp.159-170
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• 2021

Concrete filled steel tubes (CFSTs) are extensively used in a variety of structures due to their structural and economic advantages over other types of structures. Considerable research has been conducted with regards to their short-term behaviour, and very limited studies have focused on their long-term behaviour. In this study, a series of tests were carried out on high strength squat (short) CFSTs and concrete cylinders under controlled conditions of temperature and humidity to better understand their time dependent behaviour. A number of parameters were investigated including the influence of steel and concrete bond, confinement, level of sustained load and sizes of specimens. The results revealed that creep strains increased by more than 40% if there was no bonding between steel tube and concrete core. As expected, creep and shrinkage of concrete inside a steel tube were significantly less than those developed in exposed concrete. At the end of a creep period of six months, all the specimens were tested to failure to observe the influence of sustained loads on the ultimate strength. It was found that creep does not have a major effect on the strength of short CFSTs in the specific experimental study conducted here, which was less than 2.5%.

• The Korean Journal of Oral and Maxillofacial Pathology
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• v.41 no.1
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• pp.1-7
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• 2017

Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, was reported to various physiological and pharmacological effects including anti-cancer activity. Nevertheless, there is no report of the anticancer effect of lycorine in oral cancer cells. The effects of lycorine on cell proliferation and apoptosis were examined through trypan blue exclusion assay, 4'-6-diamidino-2-phenylindole (DAPI) stain, Live/Dead assay, Western blot analysis and RT-PCR. Lycorine suppressed cell viability and induced apoptosis in MC3 and HSC-3 cell lines. Lycorine decreased survivin protein but did not affect its mRNA. It regulated survivin through accelerating protein degradation in a time-dependent manner although neither proteasome nor lysosome was not associated with lycorine-mediated protein degradation. Collectively, our results suggest that lycorine may be a potential therapeutic anti-cancer drug candidate for the treatment of human oral cancer.

• The Journal of Korean Medicine
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• v.39 no.4
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• pp.158-170
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• 2018

Objectives: The objective of this study is Korean mistletoe lectin (Viscum album coloratum agglutinin, VCA) and bee venom (BV) to experimental prove comparative study of VCA and BV on the anti-cancer effect and mechanisms of action. Methods: In this study, it was examined in a human hepatocellular carcinoma cell line, Hep G2 cells. Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay in vitro. VCA and BV killed Hep G2 cells in a time- and dose-dependent manner. Results: The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action was examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including SAPK/JNK, MAPK and p38. BV also activated PARP-1, MAPK, p38 but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli. Conclusion: We examined the involvement of kinase in VCA or BV - induced apoptosis by using kinase inhibitors. VCA-induced apoptosis was partially inhibited by in the presence.

• Biomolecules & Therapeutics
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• v.27 no.5
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• pp.484-491
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• 2019

Glioblastoma is the most aggressive common brain tumor in adults. Curcumin, from Curcuma longa, is an effective antitumor agent. Although the same proteins control both autophagy and cell death, the molecular connections between them are complicated and autophagy may promote or inhibit cell death. We investigated whether curcumin affects autophagy, which regulates curcumin-mediated tumor cell death in A172 human glioblastoma cells. When A172 cells were incubated with $10{\mu}M$ curcumin, autophagy increased in a time-dependent manner. Curcumin-induced cell death was reduced by co-incubation with the autophagy inhibitors 3-methyladenine (3-MA), hydroxychloroquine (HCQ), and LY294002. Curcumin-induced cell death was also inhibited by co-incubation with rapamycin, an autophagy inducer. When cells were incubated under serum-deprived medium, LC3-II amount was increased but the basal level of cell viability was reduced, leading to the inhibition of curcumin-induced cell death. Cell death was decreased by inhibiting curcumin-induced autophagy using small interference RNA (siRNA) of Atg5 or Beclin1. Therefore, curcumin-mediated tumor cell death is promoted by curcumin-induced autophagy, but not by an increase in the basal level of autophagy in rapamycin-treated or serum-deprived conditions. This suggests that the antitumor effects of curcumin are influenced differently by curcumin-induced autophagy and the prerequisite basal level of autophagy in cancer cells.

• Journal of the Korean Society of Safety
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• v.34 no.3
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• pp.75-82
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• 2019

The purpose of this study was to evaluate the effectiveness of a vibration feedback system in improving two safe seating postures of office workers. In addition, the relative effectiveness of positive and negative feedback procedures was examined. Participants were four office workers. The dependent variables were the percentages of time participants spent in the safe sitting postures including shoulder, back, and overall body positions during the experimental sessions. A multiple-baseline design counterbalanced across participants was adopted. For two participants, the negative procedure was introduced first after baseline and positive procedure was introduced in the next phase. For the other two participants, the sequence of introducing the positive and negative feedback procedures was reversed. The result showed that the vibration feedback system, regardless of the type of the procedure, was effective in improving the safe sitting postures for all participants. However, the difference between the two procedures was not clearly shown. The benefits of the automated observation and vibration feedback system in workers' safety were discussed.

• Journal of Sensor Science and Technology
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• v.28 no.4
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• pp.236-239
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• 2019

In this work, we performed an optical simulation study on the performance of a PMDPP3T:PCBM based on an organic photovoltaic (PV) device. The virtual PV device was developed in Lumerical, finite-difference time-domain (FDTD) solutions. Different layers of the PV cell have been defined through the incorporation of complex refractive index value of those layers' constituent materials. During the simulation study, the effect of the variation active layer thickness on an ideal short circuit current density ($J_{sc,ideal}$) of the PV cell has been, first, observed. Thereafter, we have investigated the impact of surface roughness of a transparent conducting oxide (TCO) electrode on $J_{sc,ideal}$ of the PV cells. From this simulation, it has been observed that the $J_{sc,ideal}$ value of the PV cell is strongly dependent on the thickness of its active layer and the photon absorption of the PV cell has gradually decreased with the increment of the TCO's surface roughness. As a result, the capability of the PV device has been reduced with the increment of the surface roughness of the TCO.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.629-636
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• 2019

Objective: The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis. Methods: In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting. Results: The results showed that 20 to $400{\mu}M$ GA inhibited viability of TM4 Sertoli cells in a dose-dependent manner. Treatment with $400{\mu}M$ GA significantly inhibited PCNA and Cyclin B1 expression, however up-regulated BAX and Caspase-3 expression, caused mitochondrial membrane depolarization, activated Caspase-3, and induced DNA damage, thus, markedly increased the numbers of dead cells. Conclusion: Our findings showed that GA could disrupt mitochondrial function and caused TM4 cells to undergo apoptosis and necrosis.