• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.4
• /
• pp.325-327
• /
• 2001

Tobacco (Nicotiana tabacum L. cv KF109) seeds were artificially aged in a controlled environment of 45$^{\circ}C$ and 80% relative humidity condition for different duration up to 14 days before priming in polyethylene glycol 6000 solution of equivalent osmotic potential of -0.8 MPa for 8 days at $25^{\circ}C$. The seeds aged only and primed after aging were germinated at 15$^{\circ}C$ and $25^{\circ}C$ to observe the priming effects on the germination of aged seeds at different temperature. The germination percentage of the aged seeds was rapidly dropped starting from 8 days of aging and mean germination time (T$_{50}$) was greatly increased, particularly in germination at 15$^{\circ}C$. The germination capacity was greatly restored in the primed seeds after aging, particularly in the seeds of longer aging and germinating at 15$^{\circ}C$.>.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.45 no.2
• /
• pp.134-138
• /
• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• Journal of Forest and Environmental Science
• /
• v.34 no.3
• /
• pp.199-208
• /
• 2018

Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.

• Preventive Nutrition and Food Science
• /
• v.16 no.1
• /
• pp.67-73
• /
• 2011

The objective of this research was to investigate the effects of soaking time (6, 12 and 24 hr) and steeping temperature (25, 30 and $35^{\circ}C$) on germination time, germination percentage and weight loss of wheat and barley. Changes in chemical composition (such as protein, fat, and ash), reducing sugar content, enzyme activity and pasting profile and GABA ($\gamma$-animobutyric acid) content of germinated wheat and barley were also evaluated. The results clearly suggest that the short soaking time and lower steeping temperature significantly decreased germination time and weight loss, while germination percentage increased. Regarding the chemical composition, the protein content of wheat and barley was slightly increased after germination but there was no significant difference in content of crude fat and ash of both germinated cereals. The reducing sugar content of both germinated cereals decreased as the steeping temperature increased from $25^{\circ}C$ to $35^{\circ}C$. Increasing soaking time and steeping temperature led to increased amylase activity, and also corresponded to reduced paste viscosity. The highest GABA content that occurred with soaking times of 6 and 12 hr and a steeping temperature of $35^{\circ}C$ was 1,467.74 and 1,474.70 ${\mu}g/g$ for germinated wheat and 2,108.13 and 1,691.85 ${\mu}g/g$ for germinated barley. This study indicated that the optimum germination process for wheat and barley is a low steeping temperature and a short soaking time.

• Weed & Turfgrass Science
• /
• v.2 no.3
• /
• pp.274-282
• /
• 2013

Research was initiated to investigate early germination and establishment characteristics of perennial ryegrass (PR, Lolium perenne L.). An alternative condition was applied in Experiment I with 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA condition). Experiment II was conducted under $5^{\circ}C$ to $25^{\circ}C$ (natural condition). Significant differences were observed in germination percentage, germination speed and germination peak time. Seed germinating vigor was 66.00 to 93.75% under ISTA and 66.25 to 93.50% under natural conditions. Germination speed, measured as days to germination of 50% through 90%, was much faster with 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' under ISTA conditions. But it was faster only with 'Pennant II' and 'Brightstar II' under natural conditions. Germination peak time ranged 1.48 to 5.65 days under ISTA, while 2.17 to 10.63 days under natural conditions. Regardless of growing conditions, the shortest cultivars in peak time were 'Sonata', 'Pennant II', and 'Brightstar II' and the longest one 'Revenge GLX'. Considering germinating vigor, early germination speed and germination peak time, 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' were regarded as excellent cultivars under ISTA conditions, while 'Pennant II' and 'Brightstar II' under natural conditions.

• The Korean Journal of Ecology
• /
• v.19 no.3
• /
• pp.231-240
• /
• 1996

The germination responses of Cassia mimosoides var. nomame seeds to temperature were examined under various conditions. The temperature range allowing germination was $20~40^{\circ}C$, and the linear relationship between the germination rate and temperature appeared to exist between $28~38^{\circ}C$ The total thermal time required for germination (10~80%) of C. mimosoides seeds ranged from 259 Kh (degree Kelvin$\times$hours) to 421 Kh and base temperature range was relatively constant, i.e., $23.39~26.68^{\circ}C$. In the increasing temperature (IT) regime, C. mimosoides seeds started to germinate at $16^{\circ}C$ and showed greater germination rate with increasing temperatures. The final germination rate was 64% at $36^{\circ}C$. On the other hand, in the decreasing temperature (DT) regime, the seeds began to germinate at $36^{\circ}C$, and the final germination rate was 52% at $20^{\circ}C$. An induced dormancy occurred at $16^{\circ}C$ in the DT regime.

• Forest Science and Technology
• /
• v.14 no.4
• /
• pp.160-168
• /
• 2018

The seeds of Elaeocarpus serratus, a tropical underutilized fruit tree are characterized by hard seed coat and consequent poor water uptake and low germination. To improve the regeneration through seeds, various parameters such as viability of seeds, water uptake, and effect of seed mass on germination and pretreatments were performed using a completely randomized design (CRD). Tetrazolium (TZ) test was conducted using fresh, mature seeds revealed $50{\pm}2.56%$ mean viability. Seeds of different weight classes showed similar pattern of water uptake and the saturation level was achieved at 60 hrs of soaking. Seeds belong to weight class 2.6-3.5g were germinated ($12.5{\pm}1.26%$) with $175{\pm}1.75days$ (d) of mean time taken for germination (MTG). Germination capacity of seeds varied significantly among different populations and Varkala population gave $12.5{\pm}1.1%$ germination with $174.6{\pm}2.5d$ MTG. Among various seed treatments, mechanical scarification was superior in germination and significant reduction in MTG ($p{\leq}0.05$). The mechanical scarification by complete removal of seed coat resulted in $49.2{\pm}1.52%$ germination within a short period of time ($9.52{\pm}0.89d$ MTG). However, the complete removal of seed coat without damaging to embryo is a difficult task. An alternate treatment (Mechanical scarification II) by making cracks on nut faces vertically followed by soaking in distilled water for 24 hrs gave $48.4{\pm}1.73%$ germination with significantly reduced MTG ($12.14{\pm}0.56d$) over unsoaked, untreated control ($6.5{\pm}1.84%$ germination and $197.18{\pm}1.79d$ MTG; $p{\leq}0.05$). This treatment (Mechanical scarification II) is therefore recommended for E. serratus seeds as it can adopt easily and can achieve 7 fold increases in germination over control. The recorded germination through mechanical scarification is in tune with realized viability percentage of the seeds.

• The Korean Journal of Ecology
• /
• v.17 no.4
• /
• pp.485-499
• /
• 1994

This study was focused on the effect of the altitude on the geographical variations of germination characteristics in the populations of Plantago asiatica L. distribute in Mt. Chiri. There was a significant difference among the 14 groups in the phenological pattern in relation to altitudes. When the altitude becomes higher, the thermal time which was required for 10 to 80% germination rate showed higher and wider distribution. On the other hand, the germination response of increasing temperature (IT) and secreasing temperature (DT) regime was classified into 3 group. The first group was the spering germination type. This group showed that the IT regime hadhigher germination rate than that of the DT regime, and was distributed in Macheon(300m) and Packmudong (500m). The second was the spring-fall germination type which was distributed in Hadong (900m) and Saemt대 (1100m). This group also showed higher germination rate in the IT regime, but the difference of the germination rate between IT and DT regime was less than that in the first group (the spring type). The third group was the early fall germination type which was found in the Nogodan (1507m), Changetomok (1750m) and Cheonwangbong (1915m). The germination rate of this group showed almost 100% similarity between IT and DT regime. These data suggested that the geographical varations of germination characteristics within species was an important ecological strategy for the survival from severe environmental conditions.

• Korean Journal of Plant Resources
• /
• v.22 no.2
• /
• pp.111-115
• /
• 2009

The seeds of Phellodendron amurense have difficulty to obtain because these plants show dioecism with independent distributions. This experiment was conducted to find the effects of soaking and prechilling treatment on the germination of P. amurense seeds. Seeds were soaked for 3, 5 and 10 days (soaking treatment), and placed on petri-dish at $4^{\circ}C$ for 10, 20, 30 and 50 days after soaked at room temperature for 24 hours (prechilling treatment). After the treatments, germination percentage, mean germination time, germination speed and germination performance index were analyzed. As a result of sowing, prechilled seeds for 30 days had the highest percent of germination, whereas all of the soaked seeds showed lower percent of germination than non-treatment (control). In case of mean germination time, only prechilling for 30 days was more effective than control. In germination speed, all of the soaked seeds had lower values than control whereas all prechilled seeds except prechilled seeds for 20 days showed higher values than control. Especially prechilled seeds for 30 days showed the highest rate among the prechilling treatments. Germination performance index was similar to germination speed.

• Korean Journal of Plant Resources
• /
• v.27 no.3
• /
• pp.256-262
• /
• 2014

Viscum album var. coloratum (mistletoe) is considered as one of the endangered plant species in Korea. Our objective is to restore its population and multiplication of plant by ex situ method. In this research we explored the maximum germination (in vitro) of freshly harvested and stored seeds of mistletoe collected in different time intervals. Ex vitro germination after artificial inoculation on the branches of Prunus mume in different physiological conditions was also monitored. The research revealed that the lately harvested seeds (Feb. and March 2014) were superior over early harvested seeds (Nov. 2013 and Jan. 2014) of mistletoe due to the higher percentage of germination (above 93%). According to the data, it is also revealed that the survival and germination rate of mistletoe seeds decreased with the increase in storage duration. In ex vitro germination, the fluctuated temperature of a glass house in natural condition enhanced (four fold) the rate of germination on the branches of Prunus mume than the constant temperature condition in the glass house.