• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.5
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• pp.464-470
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• 2010

The integrity of the industrial equipment in use under high temperature and high pressure must be assessed by regularly measuring the degraded mechanical properties during service time. In order to nondestructively monitor the degraded mechanical properties of industrial equipment, a measuring method of the reversible permeability(RP) using surface type probe is presented. The method for measuring the RP is based on that RP is the differential value of hysteresis loop. The RP is exactly the foundation hatmonics induced in a detecting coil measured by lock-in amplifier tuned to a frequency of the alternating perturbing magnetic field. The peak of RP is measured around the coercive force. Steel material used in this work was 12Cr ferritic heat resisting steel. The eleven kinds of samples aged during different times under same temperature ($700^{\circ}C$) were prepared. Peak interval of reversible permeability(PIRP), Vickers hardness, and tensile strength measured for the aged samples decreased abruptly for short aging time (below 500 h), but the change became small at a long aging time. Vickers hardness and tensile strength linearly decreased as RIRP decreased, so the degraded mechanical properties of 12Cr ferritic heat resisting steel could be nondestructively evaluated by measuring RIRP.

• The Korean Journal of Applied Statistics
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• v.27 no.4
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• pp.589-603
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• 2014

When consecutive data follows different distributions(depending on the time interval) change-point detection infers where the changes occur first and then finds further inferences for each sub-interval. In this paper, we investigate the Bayesian detection of multiple change points. Utilizing the reversible jump MCMC, we can explore parameter spaces with unknown dimensions. In particular, we consider a model where the signal is a piecewise linear function. For the Bayesian inference, we propose a new Bayesian structure and build our own MCMC algorithm. Through the simulation study and the real data analysis, we verified the performance of our method.

• BMB Reports
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• v.34 no.1
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• pp.21-27
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• 2001

The structural stability of brain pyrydoxine-5'-phosphate (PNP) oxidase and the catalytic properties of the monomeric species were investigated. The unfolding of brain pyridoxine-5'-phosphate (PNP) oxidase by guanidine hydrochloride (GuHCl) was monitored by means of fluorescence and circular dichroism spectroscopy Reversible dissociation of the dimeric enzyme into subunits was attained by the addition of 2 M GuHCl. The perturbation of the secondary structure under the denaturation condition resulted in the release of the cofactor FMN. Separation of the processes of refolding and reassociation of the monomeric species was achieved by the immobilization method. Dimeric PNP oxidase was immobilized by the covalent attachment to Affi-gel 15 without any significant lass of its catalytic activity. Matrix-bound monomeric species were obtained from the reversible refolding processes. The matrix bound-monomer was found to be catalytically active, possessing only a slightly decreased specific activity when compared to the refolded dimeric enzyme. In addition, limited chymotrypsin digestion of the oxidase yields two fragments of 12 and 161 kDa with a concomitant increase of catalytic activity The catalytically active fragment was isolated by ion exchange chromatography and analyzed for association of two subunits using the FPLC gel filtration analysis. The retention time indicated that the catalytic fragment of 16 kDa behaves as a compact monomer. Taken together, these results are consistent with the hypothesis that the native quaternary structure of PNP oxidase is not a prerequisite for catalytic function, but it could play a role in the regulation.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.922-927
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• 2001

In an effort to regulate the mammalian cell behavior in entrapment with a gel, we have functionalized hydrogels with the putative cell-binding (-Arg-Gly-Asp-)(RGD) domain. An adhesion molecule of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, a cell recognition ligand, was induced into thermo-reversible hydrogels, composed of N-isopropylacrylamide with small amounts of acrylic acid (typically 2-5 $mol\%$ in feed), as a biomimetic extracellular matrix (ECM). The GRGDS containing a p(NiPAAm-co-AAc) copolymer gel was studied in vitro for its ability to promote the spreading and viability of cells by introducing a GRGDS sequence. Hydrogel with no adhesion molecule was a poor ECM for adhesion, permiting spreading of only $3\%$ of the seeded cells for 36h. By immobilizing the peptide linkage into the hydrogel, the conjugation of RGD promoted $50\%$ of proliferation for 36h. However, the GREDS sequence, nonadhesive peptide linkage, conjugated hydrogel showed only $5\%$ of the seeded cell for the same time period. In addition, with the serum-free medium, only GRGDS peptides conjugated to hydrogel was able to promotecell spreading, while there was no cell proliferation in the hydrogel without GRGDS. Thus, the GRGDS peptide-conjugated thermo-reversible hydrogel specifically mediated the cell spreading. This result suggests that utilization of peptide sequences conjugating with the cell-adhesive motifs can enhance the degree of cell surface interaction and influence the long-term formation of ECM in vitro.

• Carbon letters
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• v.9 no.3
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• pp.181-187
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• 2008

Batch sorption experiments were carried out for the removal of metylene blue from its aqueous solution using $H_3PO_4$ activated Acacia arabica carbon (AAC). The prepared activated carbon was characterized and was found as an effective adsorbent material. The operating variables studied were initial metylene blue concentration, AAC concentration and solution pH. AAC activated carbon posses a maximum sorption capacity for the range of initial dye concentrations studied (60~100 mg $L^{-1}$). The sorption kinetics were analyzed using reversible first order kinetics, second order, reversible first order, pseudo-first order, and pseudo-second order model. The sorption data tend to fit very well in pseudo-second order model for the entire sorption time. The average pseudo-second order rate constant, $K_{II}$ and regression coefficient value were determined to be 0.0174 mg $g^{-1}$ $min^{-1}$ and 0.9977. The biosorption process also fit well to reversible I order kinetics with a regression coefficient of 0.9878.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.6
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• pp.353-361
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• 2005

The interaction of cyclosporine A (CsA), an immunosuppressant, with rat brain Kv1.5 (Kv1.5) channels, which were stably expressed in Chinese hamster ovary cells, was investigated using the whole-cell patch-clamp technique. CsA reversibly blocked Kv1.5 currents at +50 mV in a reversible concentrationdependent manner with an apparent $IC_{50}$ of 1.0μM. Other calcineurin inhibitors (cypermethrin, autoinhibitory peptide) had no effect on Kv1.5 and did not prevent the inhibitory effect of CsA. Fast application of CsA led to a rapid and reversible block of Kv1.5, and the onset time constants of the CsA-induced block were decreased in a concentration-dependent manner. The CsA-induced block of Kv1.5 channels was voltage-dependent, with a steep increase over the voltage range of channel opening. However, the block exhibited voltage independence over the voltage range in which channels were fully activated. The rate constants for association and dissociation of CsA were $7.0{\mu}M{-1}s^{-1}$ and $8.1s^{-1}$, respectively. CsA slowed the deactivation time course, resulting in a tail crossover phenomenon. Block of Kv1.5 by CsA was use-dependent. CsA also blocked Kv1.3 currents at +50 mV in a reversible concentration-dependent manner with an apparent $IC_{50}$ of $1.1{\mu}M$. The same effects of CsA on Kv1.3 were also observed in excised inside-out patches when applied to the internal surface of the membrane. The present results suggest that CsA acts directly on Kv1.5 currents as an open-channel blocker, independently of the effects of CsA on calcineurin activity.

• Journal of the Korean Statistical Society
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• v.22 no.2
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• pp.259-269
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• 1993

A moving average model, LMA(q) and an autoregressive-moving average model, NLARMA(p, q), with Laplacian marginal distribution are constructed and their properties are discussed; Their autocorrelation structures are completely analogus to those of Gaussian process and they are partially time reversible in the third order moments. Finally, we study the mixing property of NLARMA process.

• 한국정보디스플레이학회:학술대회논문집
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• 2006.08a
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• pp.1436-1441
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• 2006

Each type of optical sheets in a LCD module experiences a characteristic behavior for thermal loading and unloading. During thermal cycling, a polymeric behavior is reversible and recyclable, depending on a material stiffness critically affected by temperature and time. Some critical issues on temperature- and time-dependent themomechanical deformation of the polymeric sheet are addressed by finite-element thermal results, followed by structural simulation results in this study.

• Asia pacific journal of information systems
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• v.26 no.3
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• pp.427-448
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• 2016

The stochastic phenomena of traffic network condition, such as traffic speed and density, are affected not only by exogenous traffic control but also by endogenous changes in service time during congestion. In this paper, we propose a mixed M/G/1 queuing model by introducing a condition-varying parameter of traffic congestion to reflect structural changes in the traffic network. We also develop congestion indices to evaluate network efficiency in terms of traffic flow and economic cost in traffic operating system using structure-changing queuing model, and perform scenario analysis according to various traffic network improvement policies. Empirical analysis using Korean highway traffic operating system shows that our suggested model better captures structural changes in the traffic queue. The scenario analysis also shows that occasional reversible lane operation during peak times can be more efficient and feasible than regular lane extension in Korea.

• Solar Energy
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• v.9 no.2
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• pp.22-30
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• 1989

Since the energy storage method by means of the thermochemical reaction has no heat loss by separating the reactants under the storage period, it is remarked as one of promising means particularly for long-term heat storage. In this study, the heat-storage/-release characteristics of the reversible chemical reaction cycle, $Ca(OH)_2/CaO$, is numerically analysed by a mathematical modelling. As a result, the effectiveness of the heat exchanger by the chemical heat storage method is considerably higher than that by the sensible heat storage method. It is found that the major parameters, which determines the effectiveness of the heat exchanger, are the mass flow rate and inlet temperature of fluid, the residence time, etc.. The heat-storage/-release period can be controlled by changing the operation conditions. It is expected that the results obtained here will supply useful informations in designing a regenerative heat exchanger utilizing the thermochemical reaction.