• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.157-162
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• 2012

To analyze nucleotide sequence encoding internal transcribed spacer (ITS) regions specific to the Laminariaceae family, genomic DNA was isolated from six brown algae species distributed along the east coast of Korea. These included three species from the Laminariaceae family (Agarum clathratum Dumortier, Costaria costata [C. Agardh] Saunders, and Saccharina japonica Areschoug) and two species from the Alariaceae family (Undaria pinnatifida [Harvey] Suringer and Ecklonia cava Kjellman), both in the order Laminariales, and one species from the family Sargassaceae in the order Fucales (Sargassum serratifolium). Based on a sequence analysis of ITS-1 and ITS-2 for A. clathratum, C. costata, and E. cava, oligonucleotides were designed from the regions that showed sequence conservation in Laminariaceae. Following polymerase chain reaction using three sets of primers, amplification of ITS-1 and ITS-2 was detected in reactions using genomic DNA isolated from the species belonging to Laminariaceae, but not from the species belonging to the other families. The results indicate that this method can be used for the detection and identification of Laminariaceae species.

• Animal Systematics, Evolution and Diversity
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• v.30 no.1
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• pp.49-54
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• 2014

Korean species of the subfamily Lycorininae Cushman & Rohwer are reviewed. This subfamily along with three species, Lycorina triangulifera Holmgren, 1859, L. spilonotae Chao, 1980, and L. ruficornis Kasparyan, 2007, are reported for the first time from Korea. In this genus, five species have been reported from the Eastern Palaearctic region, six species from China and two species from Japan. Some species of this subfamily is known as parasitoids of Crambidae and Tortricidae. We report three newly recorded species from Korea, Lycorina triangulifera, L. spilonotae, and L. ruficornis, and provide diagnoses with photographs of lycorinine species and a key to the Korean species.

• Journal of Microbiology
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• v.38 no.2
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• pp.62-65
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• 2000

Partial sequence comparisons of mitochondrial small subunit rDNA (mt SSU rDNA) were used to examine taxonomic and evolutionary relationships among seven Penicillium species : two monoverticillate species, two biverticillate species, and three terverticillate species. Amplified fragments of mt SSU rDNA highly varied among seven species in size, suggesting the existence of multiple insertions or deletions in the region. A phylogengtic tree was constructed by exhaustive search of parsimony analysis. The phylogenetic tree distinguished two statistically supported monophyletic groups, one for two monoverticillate species and the other for three terverticillate species and ont biverticillate species, P. vulpinum. The phylogenetic relationship of P. waksmanii, the biverticillate species, was not clear.

• Animal Systematics, Evolution and Diversity
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• v.37 no.1
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• pp.70-77
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• 2021

The genus Parorthocladius is a small group among the subfamily Orthocladiinae (Diptera: Chironomidae). A total of ten species of Parorthocladius have been recorded in the world, of which two species were found from Japan, three species from China, and three species from the Russian Far East. Only one species, P. nudipennis is widely distributed in Europe and the Russian Far East. This genus can be distinguished from other genera by the absence of acrostichals on the thorax, wing vein R4+5 ending proximal to the tip of vein M3+4, anal point parallel side, rounded apically. We report four new species of Parorthocladius collected in Korea and described them with illustrations. As a result, fourteen species of Parorthocladius have been recorded in the world.

• Mycobiology
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• v.31 no.2
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• pp.74-80
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• 2003

Isolates of Trichoderma spp. collected from Pleurotus ostreatus and P. eryngii beds, which included loosened substrate compactness and development of green colour, were grouped into three species. The occurrence of different species of Trichoderma was as T. cf. virens(70.8%), T. longibrachiatum(16.7%) and T. harzianum(12.5%). The conidia of Trichoderma spp. were ellipsoidal, obovoid and phialides were bowling pins, lageniform and the length of phialides was $3.5{\sim}10.0{\times}1.3{\sim}3.3{\mu}m$. Phialides of T. cf. virens and T. harzianum were tending clustered, but it was solitary disposition in T. longibrachiatum. T. cf. virens was characterized by predominantly effuse conidiation, sparingly branched, and fertile to the apex and it was penicillate type. RAPD analysis could detect variability amongst three different species of Trichoderma using two newly designed URP-primers. However, intra-specific variation could not be detected in all the isolates except for rDNA sequence data classified Trichoderma isolates into three distinct groups representing three species. The profiles of rDNA sequences of isolates representing a species showed high similarity in T. cf. virens and T. harzianum. However, there was a variation in rDNA sequences of isolates representing T. longibrachiatum. The results of present study reveals that molecular techniques of RAPD and rDNA sequencing can greatly aid in classification based on morphology and precise identification of fast evolving species of Trichoderma.

• ALGAE
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• v.34 no.4
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• pp.267-275
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• 2019

Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

• Animal Systematics, Evolution and Diversity
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• v.33 no.2
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• pp.65-72
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• 2017

This study reports three species in the genus Tubastraea from Korea: Tubastraea coccinea Lesson, 1829; Tubastraea faulkneri Wells, 1982; and Tubastraea micranthus (Ehrenberg, 1834). Tubastraea faulkneri and T. micranthus are newly recorded in Korea. The specimens of three species were collected in the subtidal zones off Jeju-do between 1991 and 2010. The two newly recorded species were described in detail based upon the morphological characters of skeletal structures. The previous records of T. coccinea in Korea were supplemented with additional data in the remarks. These three species have a straight septal arrangement or irregular septal fusion in common as a main character for the genus Tubastraea, but they differ with respect to the growth form, intercorallite distance, exsertness from common coenosteum, and the detailed characters of septal arrangement. Tubastraea faulkneri is similar to T. coccinea in its plocoid growth form, well developed common coenosteum, and corallite size. However, unlike the latter species, the former species is characterized by rare or absent budding adjacent to the corallite edges, wider intercorallite distance, and irregularly developed septal fusion near the columella. In particular, T. micranthus is distinguished by an axial dendroid growth form, and the smallest corallites in this genus.

• ALGAE
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• v.33 no.1
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• pp.69-84
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• 2018

We examined the species diversity of Herposiphonia on Korean coasts, based on a combination of morphology and molecular analyses of the mitochondrial COI-5P DNA barcode marker and plastid rbcL gene. We report the presence of eight species including three novel species: H. donghaensis sp. nov., H. jejuinsula sp. nov., H. sparsa sp. nov., H. caespitosa, H. fissidentoides, H. insidiosa, H. parca, and H. subdisticha. Specimens were separated into eight clades in both the COI-5P and rbcL gene analyses, with 1.3-19.6 and 6.6-15% interspecific sequence divergence, respectively. These eight species are also distinguishable by several morphological characteristics such as: branching pattern (d/i pattern in H. donghaensis sp. nov. and H. sparsa sp. nov.; d/d/d/i pattern in others), shape of determinate branch (ligulate in H. fissidentoides; terete in others), number of vegetative trichoblasts (1-2 in H. insidiosa and H. sparsa sp. nov.; 3-4 in H. caespitosa; absent in others), and number of segments and pericentral cells in determinate branches. About three novel species revealed by our analyses, H. donghaensis sp. nov. is newly discovered, and H. jejuinsula sp. nov. and H. sparsa sp. nov. were previously reported in Korea as H. nuda and H. secunda, respectively. Our results show that DNA barcoding and rbcL analyses are useful for delimiting species boundaries and discovering cryptic species diversity in the genus Herposiphonia.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.3
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• pp.71-87
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• 2007

In order to improve and manage rationally for Youngdam creek in Haman-gun, the channel change, water quality and ecological adaptation etc. were evaluated by monitoring during three years. The distinct differences of channel characteristics appeared in the extents of bed excavation and the revetment methods because overall reconstruction was occurred intense disturbance of width expansion and levee construction etc. The scour and deposition were caused according to channel characteristics of ripple and pool, and the adaptation of vegetation was distinguished from each revetment methods clearly. Water quality was maintained first class on the basis of BOD as 0.5~1.5mg/${\ell}$. The vegetation appeared in 380 species close-to-nature river improvement before, but 64 species it after one year, 159 species it after two year and 158 species it after three year. The animal life appeared in 123 species of close-to-nature river improvement before, but 103 species it after one year, 116 species it after two year and 119 species it after three year.

• Animal cells and systems
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• v.6 no.1
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• pp.41-43
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• 2002

A new parasmittinid bryozoan, Parasmittina pyriformis, is reported from four localities in Korea. This new species closely resembles Parasmittina trispinosa in having one to three spines and a triangular avicularium lateral to the orifice. However, P. pyriformis is characteristic in that a single or paired small pear-shaped avicularia are situated lateral to the orifice and a triangular avicularium occurs in laterals to the orifice or on the frontal wall. The new species is also unique in having two spines in most zooids, but rarely one or three spines, whereas P. trispinosa usually has three spines, sometimes two or four.