• Advances in Computational Design
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• v.4 no.2
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• pp.179-196
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• 2019

Temporary scaffold systems in the construction site play an important role for ensuring the safety of the workers and for constructing the stable structures. To assemble the scaffold, the pipe scaffolding system, the wedge binding scaffolding system and the particular materials have been utilized. To design the material arrangement of a scaffold, firstly the configuration was determined considering the construction geometry. Then, the strength of the scaffold was confirmed and the quantity of the material was accounted. In this paper, the design method of the temporary scaffold was proposed for intending the semi-automatic procedure. In the proposed design method, the geometric design and the safety requirement were specified by the safety standard and the design flow was followed by the designer's knowledge. The size and the quantities of the materials were calculated by referring to the relation between the scaffold and the constructing structure. In the calculating procedure, three dimensional positions of each scaffold materials were calculated and recorded simultaneously. Then, three dimensional scaffold structural was drawn semi-automatically on the CAD software by using the obtained material sizes, positions and directions. The proposed design method provides us the precise quantities of scaffold materials and enables us to reduce the design effort and the cost estimation processes. In addition, the obtained results can be applied to BIM software after converting to IFC format.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.651-651
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• 2013

Templated strategy is a very powerful tool for creating multi-dimensional self assembly of nanomaterials. Since viral protein cages have a uniform size with a well-defined structure, they can serve as an excellent template for the formation of a three-dimensional self-assembly of synthetic nanoparticles. In this study, we have examined the feasibility of the 3D self-assembly of gold nanoparticles of various sizes using a brome mosaic virus (BMV) capsid with cysteine groups expressed on its surface as a scaffold for the assembly. It was found that the three-dimensional clusters of gold nanoparticles with a designed structure were attainable by this approach, which was verified by transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.5
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• pp.311-325
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• 2001

Tissue engineering scaffolds play a critical role in regulating the reconstructed human tissue development. Various types of scaffolds have been developed in recent years, including fibrous matrix and foam-like scaffolds. The design of scaffold materials has been investigated extensively. However, the design of physical structure of the scaffold, especially fibrous matrices, has not received much attention. This paper compares the different characteristics of fibrous and foam-like scaffolds, and reviews regulatory roles of important scaffold properties, including surface geometry, scaffold configuration, pore structure, mechanical property and bioactivity. Tissue regeneration, cell organization, proliferation and differentiation under different microstructures were evaluated. The importance of proper scaffold selection and design is further discussed with the examples of bone tissue engineering and stem cell tissue engineering. This review addresses the importance of scaffold microstructure and provides insights in designing appropriate scaffold structure for different applications of tissue engineering.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.7
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• pp.669-674
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• 2013

A three-dimensional hydrogel scaffold has been proposed for the effective production of biomimetic intestinal villi to reduce ethical and cost problems caused by animal testing in pharmaceutical development. This study explores an experimental approach to develop a new technique based on laser machining and microdrilling processes to produce a plastic mold for the fabrication of a three-dimensional hydrogel scaffold. For process optimization, both the laser machining and the microdrilling experiments are conducted by varying the experimental conditions, and structural characterization of the mold and intestinal villi were performed using SEM (scanning electron microscope) and OM (optical microscope) images. Polycarbonate (PC) was used as a candidate material. The experimental results show that intestinal villi can be fabricated by both laser and microdrilling machining processes.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.11a
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• pp.597-598
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• 2008

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.1459-1460
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• 2011

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.1265-1270
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• 2007

Conventional methods for fabricating three-dimensional (3-D) scaffolds have substantial limitations. In this paper, we present 3-D scaffolds that can be made repeatedly with the same dimensions using a microstereolithography system. This system allows the fabrication of a pre-designed internal structure, such as pore size and porosity, by stacking photopolymerized materials. The scaffolds must be manufactured in a material that is biocompatible and biodegradable. In this regard, we synthesized liquid photocurable biodegradable TMC/TMP, followed by acrylation at terminal ends. And also, solidification properties of TMC/TMP polymer are to be obtained through experiments. Cell adhesion to scaffolds significantly affects tissue regeneration. As a typical example, we seeded chondrocytes on two types of 3-D scaffold and compared the adhesion results. Based on these results, the scaffold geometry is one of the most important factors in chondrocyte adhesion. These 3-D scaffolds could be key factors for studying cell behavior in complex environments and eventually lead to the optimum design of scaffolds for the regeneration of various tissues, such as cartilage and bone.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.4
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• pp.259-269
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• 2022

Objective: Animal-free scaffolds have emerged as a potential foundation for consistent, chemically defined, and low-cost materials. Because of its good potential for high biocompatibility with reproductive tissues and well-characterized scaffold design, we investigated whether polyglycolic acid (PGA) could be used as an animal-free scaffold instead of natural fibrin-agarose, which has been used successfully for three-dimensional human endometrial cell culture. Methods: Isolated primary endometrial cells was cultured on fibrin-agarose and PGA polymers and evaluated various design parameters, such as scaffold porosity and mean fiber diameter. Cytotoxicity, scanning electron microscopy (SEM), and immunostaining experiments were conducted to examine cell activity on fabricated scaffolds. Results: The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and SEM results showed that endometrial cells grew and proliferated on both scaffolds. Immunostaining showed cytokeratin and vimentin expression in seeded cells after 7 days of culture. On both scaffolds, an epithelial arrangement of cultured cells was found on the top layer and stromal arrangement matrix on the bottom layer of the scaffolds. Therefore, fibrin-agarose and PGA scaffolds successfully mimicked the human endometrium in a way suitable for in vitro analysis. Conclusion: Both fibrin-agarose and PGA scaffolds could be used to simulate endometrial structures. However, because of environmental and ethical concerns and the low cost of synthetic polymers, we recommend using PGA as a synthetic polymer for scaffolding in research instead of natural biomaterials.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.231-235
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• 2005

Most tissue engineering strategies for creating functional replacement tissues or organs rely on the application of temporary three-dimensional scaffolds to guide the proliferation and spread of seeded cells in vitro and in vivo. Scaffolds should be satisfied following requirements; macrostructure to promote cell proliferation, pore interconnectivity, pore size ranging from 200 to $400{\mu}m$, surface chemistry and mechanical properties. Rapid prototyping techniques have often been used as an useful process that fabricates scaffolds with complex structures. In this study, a new process to fabricate a three-dimensional scaffolds using bio-compatible polymer has been developed. It employs a highly accurate three-dimensional positioning system with pressure-controlled syringe to deposit biopolymer structures. The pressure-activated microsyringe is equipped with fine-bore nozzles of various inner-diameters. In order to examine relationships between line width and process parameters such as nozzle height, applied pressure, and speed of needle, experiments were carried out. Based on the experimental results, three-dimensional scaffold was fabricated using the apparatus. It shows the validity of the proposed process.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.7
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• pp.834-850
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• 2011

In this paper, a novel tissue engineering scaffold design method based on triply periodic minimal surface (TPMS) is proposed. After generating the hexahedral elements for a 3D anatomical shape using the distance field algorithm, the unit cell libraries composed of triply periodic minimal surfaces are mapped into the subdivided hexahedral elements using the shape function widely used in the finite element method. In addition, a heterogeneous implicit solid representation method is introduced to design a 3D (Three-dimensional) bio-mimetic scaffold for tissue engineering from a sequence of computed tomography (CT) medical image data. CT image of a human spine bone is used as the case study for designing a 3D bio-mimetic scaffold model from CT image data.