• BMB Reports
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• 제43권12호
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• pp.789-794
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• 2010

A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-${\beta}1$ and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.

• 대한한방내과학회지
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• 제27권2호
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• pp.371-378
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• 2006

Objectives : The aim of this study is to review the effects of acupuncture on lower limb spasticity control in stroke patients. Methods: H-reflex/M-response ratio and Modified Ashworth Scale was used for evaluation of spasticity control before acupuncture, within one hours after acupuncture , at twenty-four hours, and four weeks. We divided two groups. One is the yin meridian acupunctured goup and the other is the yang meridian acupunctured group. Results: H/M ratio is declined within 1 or 24 hours after acupuncture for poststroke spasticity patients. The yin meridian acupunctured group is a bit more efficient than the yang meridian acupunctured goup. But there is no meaning in the statistics because of small patient pool. Conclusions: We need more patients group to decide the effect of electro- acupuncture on leg spasticity of stroke patients.

• 대한한방내과학회지
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• 제27권2호
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• pp.488-499
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• 2006

Objectives : This study was executed in order to examine relationship between Sasang constitution and serum lipids in CVA patients. Method and Results : This study was done with 104 cases of stroke patients who admitted to department of the internal medicine Wonkwang university oriental hospital from September in 2004 to August in 2005. A study on the relationship among serum lipids : In T-cholesterol and triglyceride, T-cholesterol and HDL-cholesterol, LDL-cholesterol and HDL-cholesterol. T-cholesterol and LDL-cholesterol, they had positive correlation each other. Between triglyceride and HDL-cholesterol, they had negative correlation each other. Distribution of serum lipids which it follows in stroke type : T-cholesterol, HDL-cholesterol and LDL-cholesterol average of cerebral hemorrhage is higher than cerebral infarction. Distribution of serum lipids which it follows in Sasang constitution : T-cholesterol, triglyceride and LDL-cholesterol average of Taeyumin was higher than different types of Sasang constitution But it didn't have no statistically consideration Conclusions : The relationship between Sasang constitution and Serum lipids was not proven.

• Molecules and Cells
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• 제39권2호
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• pp.96-102
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• 2016

miR-101 is considered to play an important role in hepatocellular carcinoma (HCC), but the underlying molecular mechanism remains to be elucidated. Here, we aimed to confirm whether Girdin is a target gene of miR-101 and determine the tumor suppressor of miR-101 through Girdin pathway. In our previous studies, we firstly found Girdin protein was overexpressed in HCC tissues, and it closely correlated to tumor size, T stage, TNM stage and Edmondson-Steiner stage of HCC patients. After specific small interfering RNA of Girdin was transfected into HepG2 and Huh7.5.1 cells, the proliferation and invasion ability of tumor cells were significantly inhibited. In this study, we further explored the detailed molecular mechanism of Girdin in HCC. Interestingly, we found that miR-101 significantly low-expressed in HCC tissues compared with that in matched normal tissues while Girdin had a relative higher expression, and miR-101 was inversely correlated with Girdin expression. In addition, after miR-101 transfection, the proliferation, migration and invasion abilities of HepG2 cells were weakened. Furthermore, we confirmed that Girdin is a direct target gene of miR-101. Finally we confirmed Talen-mediated Girdin knockout markedly suppressed cell proliferation, migration and invasion in HCC while downregulation of miR-101 significantly restored the inhibitory effect. Our findings suggested that miR-101/Girdin axis could be a potential application of HCC treatment.

• Molecules and Cells
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• 제37권11호
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• pp.812-818
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• 2014

This study was to investigate the mechanism and role of Kif4A in doxorubicin-induced apoptosis in breast cancer. Using two human breast cancer cell lines MCF-7 (with wild-type p53) and MDA-MB-231 (with mutant p53), we quantitated the expression levels of kinesin super-family protein 4A (Kif4A) and poly (ADP-ribose) Polymerase-1 (PARP-1) by Western blot after doxorubicin treatment and examined the apoptosis by flow cytometry after treatment with doxorubicin and PARP-1 inhibitor, 3-Aminobenzamide (3-ABA). Our results showed that doxorubicin treatment could induce the apoptosis of MCF-7 and MDA-MB-231 cells, the down-regulation of Kif4A and upregulation of poly(ADP-ribose) (PAR). The activity of PARP-1 or PARP-1 activation was significantly elevated by doxorubicin treatment in dose- and time-dependent manners (P < 0.05), while doxorubicin treatment only slightly elevated the level of cleaved fragments of PARP-1 (P > 0.05). We further demonstrated that overexpression of Kif4A could reduce the level of PAR and significantly increase apoptosis. The effect of doxorubicin on apoptosis was more profound in MCF-7 cells compared with MDA-MB-231 cells (P < 0.05). Taken together, our results suggest that the novel role of Kif4A in doxorubicin-induced apoptosis in breast cancer cells is achieved by inhibiting the activity of PARP-1.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제48권6호
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• pp.371-381
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• 2022

Objectives: This study determined the effect of platelet-rich fibrin (PRF) on extraction socket bone regeneration and assessed the patterns and determinants of bone regeneration after the surgical extraction of impacted mandibular third molars. Materials and Methods: This prospective study randomly allocated 90 patients into two treatment groups: A PRF group (intervention group) and a non-PRF group (control group). After surgical extractions, the PRF group had PRF placed in the extraction socket and the socket was sutured, while the socket was only sutured in the non-PRF group. At postoperative weeks 1, 4, 8, and 12, periapical radiographs were obtained and HLImage software was used to determine the region of newly formed bone (RNFB) and the pattern of bone formation. The determinants of bone regeneration were assessed. Statistical significance was set at P<0.05. Results: The percentage RNFB (RNFB%) was not significantly higher in the PRF group when compared with the non-PRF group at postoperative weeks 1, 4, 8, and 12 (P=0.188, 0.155, 0.132, and 0.219, respectively). Within the non-PRF group, the middle third consistently exhibited the highest bone formation while the least amount of bone formation was consistently observed in the cervical third. In the PRF group, the middle third had the highest bone formation, while bone formation at the apical third was smaller compared to the cervical third at the 8th week with this difference widening at the 12th week. The sex of the patient, type of impaction, and duration of surgery was significantly associated with percentage bone formation (P=0.041, 0.043, and 0.018, respectively). Conclusion: Placement of PRF in extraction sockets increased socket bone regeneration. However, this finding was not statistically significant. The patient's sex, type of impaction, and duration of surgery significantly influenced the percentage of bone formation.

• International Journal of Knowledge Content Development & Technology
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• 제13권1호
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• pp.59-74
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• 2023

Third places are those that offer a space outside of work and home for people to escape the stress associated with the primary spaces of their lives. Through social interactivity these help in acquiring some emotional support provided by a close and intimate attachment. Such social support can turn into social resources for individuals and can lead to forming communities that may become incubators for social capital through regular visits to shared socializing places. This paper focuses on social interaction that takes place through the third place and the communities that are being created as a contributing factor to knowledge management. The study being reported in this paper investigated the role of coffee shops as third places in building communities and social capital in Kuwait. A mixed-method approach was used to conduct the study. These methods included qualitative methods such as extended conversations in the form of semi-structured interviews and quantitative methods of using questionnaires distributed to coffee shop staff and visitors. Results indicated that importance attached to providing facilities by coffee shops reflected that the owners perceived these as third places. The facilities attracted customers to coffee houses where interactions took place that helped to build communities. The pattern of visits by customers showed that mostly they come in groups and have meetings and gatherings that facilitated and encouraged interactions. The communities that are being built in the coffee shops are indicative of these being perceived as third places by owners, customers, and staff. This study shows the potential of coffee shops as a major sector of the food industry promising a valuable contribution in transformation and transition to a knowl- edge-based economy in Kuwait.

• Journal of Gastric Cancer
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• 제24권2호
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• pp.159-171
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• 2024

Purpose: Gastric cancer is one of the most common cancers in Korea, and the proportion of upper-third gastric cancers has been steadily increasing over the last two decades. This study aimed to evaluate the effect of tumor location on gastric cancer prognosis. Materials and Methods: We retrospectively reviewed 2,466 patients who underwent gastrectomy for pathologically proven gastric cancer between January 2011 and December 2016. The patients were divided into an upper-third group (U group; n=419, 17.0%) and a middle- and lower-third group (ML group; n=2,047, 83.0%). Clinicopathological characteristics, overall survival (OS), and recurrence-free survival (RFS) after surgery were compared. Results: The U group had more advanced disease than the ML group and a higher incidence of N3b disease for T3 (12.0% vs. 4.9%, p=0.023) and T4 tumors (33.3% vs. 17.5%, p=0.001). The 5-year RFS rate for stage III disease was marginally lower in the U group than that in the ML group (47.1% vs. 56.7%, p=0.082). The upper third location was an independent prognostic factor for both OS (hazard ratio [HR], 1.350; 95% confidence interval [CI], 1.065-1.711) and RFS (HR, 1.430; 95% CI, 1.080-1.823). Conclusions: Upper-third gastric cancer shows extensive node metastasis compared to those located more distally in ≥T3 tumors. The upper third location is an independent prognostic factor for both OS and RFS and may have an adverse impact on RFS, particularly in patients with stage III gastric cancer.

• Journal of applied mathematics & informatics
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• 제28권3_4호
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• pp.877-882
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• 2010

In this paper, a third-order three-point boundary value problem on time scales is considered. We establish criteria for the existence of a solution and a positive solution by using the Leray-Schauder fixed point theorem. An example is also given to illustrate our results.

• 충청수학회지
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• 제26권1호
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• pp.167-174
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• 2013

The main goal in the present paper is to obtain a solution of Einstein's unified field equations for the third class in $X_4$.