• 한국환경과학회지
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• 제18권11호
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• pp.1207-1214
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• 2009

Functional properties of Solanium nigrum-ethanol extract were investigated, nitrite scavenging ability(NSA) and antioxidant activities based on thiobarbituric acid(TBA) value were measured. The contents of potassium in fruit and root, calcium in leaf were the highest mineral components in each parts of Solanium nigrum. Total aromatics contents(TAC) was order of fruit>root>leaf, total flavonoid contents(TFC) in leaf was 6 and 10 times, total phenolic compounds contents(TPC) in leaf was 15 and 30 times of those in fruit and root, respectively, and TFC was high with TPC increase. NSA of leaf-ethanol extract was 61.9% on pH 1.2, 53.5% on pH 3.0, 54.8% on pH 4.0 and 14.3% on pH 6.0, where as NSA of root and fruit-ethanol were considerably low to 3.5~7.9% over pH 1.2~6.0. TBA value of soybean oil with the dosage of leaf-extract showed few differences from control within 3 days, but it fairly decreased with considerable antioxidant effect to 47.6% of the control in 8 days.

• 한국작물학회지
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• 제45권5호
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• pp.328-334
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• 2000

Sixty local soybean cultivars were evaluated on the antioxidative activity by superoxide dismutase (SOD), 1, 1-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid(TBA), and chemiluminescence using the FI-CL system. Soybean were collected throughout the country, and were grown over two years (1997 and 1998) for measuring antioxidative activity in soybean seeds. There were differences in antioxidative activity depen-ding on the method of measurement and variation of the crop year. Soybeans from Kwangyang-shi-1 (76.78%) in 1997 and Kangjin-gun-3(79.14%) in 1998 showed the highest SOD activity, whereas those from Hwasoon-gun (80.43%) in 1997 and Kangjin-gun-2 (49.82 %) in 1998 exhibited the highest DPPH activity. Soybeans from Chongup-gun-2 (75.77%) in 1997 and from Yochon-shi-5 (69.17%) in 1998 exhibited the highest TBA activity, and those from Jinahn-gun (48.99%) in 1997 and Kohung-gun (49.73%) in 1998 exhibited the highest activity using the chemiluminescence method. These results suggest that it may be possible to develop soybean varieties with higher antioxidative activity.

• 한국축산식품학회지
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• 제36권3호
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• pp.300-308
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• 2016

In this study, the effects of grape seed flour on the physical-chemical properties, microbiological and sensory properties of Turkish dry fermented sausage, sucuk, was investigated. After the sausages produced with beef, beef fat, sheep tail fat and spices, they were ripened for 14 d. Then they were vacuum-packaged and stored for 80 d at 4℃. The effects of grape seed flour (GSF; 0%, 0.75%, 1.5%, 3%) on the physical-chemical properties (pH, moisture, fat, protein, free fatty acids, thiobarbituric acids, diameter reduction, ripening yield, instrumental colour), microbiological properties (total aerobic mesophilic and lactic acid bacteria, Enterobacteriaceae, mould and yeast) and sensory properties of the sausages were investigated. Grape seed flour decreased moisture, TBA, diameter reduction, instrumental colour (a, b) values and sensory analysis scores during the ripening period; it also decreased TBA, instrumental colour (L, a, b) values, total aerobic mesophilic and lactic acid bacteria counts during the storage period. It was concluded that grape seed flour has a potential application as an additive in dry fermented sausages.

• 생약학회지
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• 제29권1호
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• pp.13-17
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• 1998

The root of Aralia continentalis Kitagawa (Araliaceae) have been used as an analgesic and fever remedy, and for treatment of rheumatism in Chinese medicine, whereas the young leaves are used for ingredient of salad. Antioxidant activity of the young leaves of A. continentails was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA) and by evaluation the radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Chromatographic separation of active fraction led to the isolation of six flavonoids, among which quercetin, hyperoside and kaempferol showed strong antioxidant activities, while 6"-O-acetyl astragalin, astragalin and trifolin were inactive. Adenosine, oleanolic acid 28-O-glucosyl ester and salsoloside C methyl ester isolated from the somewhat active BuOH fraction exhibited no antioxidant activities.

• Plant Resources
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• 제7권1호
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• pp.47-53
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• 2004

Several wild plant species are known to contain biologically active substances that are allelopathic to weed species as well as antioxidant to foods. Plant extracts or residues from leaves of 4 species, Achyranthes japonica (speedwell), Cucumis sativus (Cucumber), Trifolium repens (white clover), and Vicia angustifolia (narrowleaf vetch) were bioassayed against Medicago sativa (alfalfa) or Echinochloa crus-galli (barnyard grass) to determine their allelopathic effects, and used for measurement of antioxidant activities. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa. Aqueous extracts or residues from V. angustifolia showed the most inhibitory effect on alfalfa or barnyard grass seedling growth and followed by A. japonica and T. repens. Oxidative stability by Rancimat method, antioxidant activity by TBA (2-thiobarbituric acid) method and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity for the ground samples or methanol extracts were the greatest in V. angustifolia, although were less than those of commonly used antioxidants, BHT (butylated hydroxytoluene) and ascorbic acid. These results suggest that the wild plant species had potent allelopathic and antioxidant activities, and that their activities differed depending on plant species.

• 한국식품저장유통학회지
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• 제9권3호
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• pp.351-355
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• 2002

목초액의 아질산염 소거, 전자공여작용 및 대두유에 농도별(0.5, 1 및 5%)로 첨가하여 항산화성을 조사하였다. 아질산염 소거 효과는 모든 농도에서 pH가 증가하면서 감소하는 경향을 보였고, pH 1.2의 경우 100 ${\mu}\ell$/mL의 농도에서 80%이상의 높은 활성을 보였다. 전자공여작용은 50 ${\mu}\ell$/mL의 농도에서 약 80%의 효과를 나타내었다. 목초액을 첨가하지 않은 대두유를 control로 사용하였고, 0.1% BHT를 첨가한 대두유는 비교군으로 사용하여 5$0^{\circ}C$에서 10일간 저장하면서 실험을 하였다. 과산화물가는 목초액 1%와 5% 첨가군이 control과 BHT 첨가군보다 낮게 나타났으며, TBA가는 5% 목초액에서 가장 낮았고, 1% 목초액, 0.1% BHT 및 0.5% 목초액 첨가군 순으로 나타났다. 산가는 5% 목초액 첨가군이 가장 낮았고, 0.1% BHT, 1% 및 0.5% 목초액의 순으로 낮게 나타났다.

• 한국축산식품학회지
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• 제24권3호
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• pp.298-302
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• 2004

EM 함초 발효액의 항산화 효과에 대한 연구를 수행하였다. 함초의 무기질 함량은Na의 경우 높은 함량을 나타났으며 K과 Mg의 경우 자생지에 관계없이 각 부위별로 거의 비슷한 함량을 보였으나, 동결 건조한 시료가 온풍 건조한 시료보다 훨씬 높은 함량을 나타내었다. Ca 함량은 뿌리보다 잎에서 2배 이상 높은 함량을 보였으며, Fe은 함초의 뿌리에 많이 함유되어 있었다. 해안에서 채취한 함초의 동결 건조시킨 잎이 20.19%로 가장 높은 함량을 보여 구운 소금의 약1/5에 미치는 식염 함량을 나타냈다. 함초의 뿌리 부분을 EM으로 발효시킨 EM 함초 발효액이 저장 14일에는 대조구 보다 5.3배 정도의 항산화 효과를 나타냈다.

• 한국축산식품학회지
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• 제32권1호
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• pp.98-102
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• 2012

본 연구는 TBARS 및 PV 등의 식품중의 지질산패도 측정법을 대체할 수 있는 간단한 측정법을 개발하고자 시행되었다. 이를 위해 선행 연구에서 식용유지의 산패도 측정법 대체가능성을 나타낸 화학발광도 측정법을 고체시료 중에서 축산식품인 동결건조 난황분말에 적용하여 기존의 산패도 측정법과의 상관관계를 조사하여 대체 가능성을 확인하였다. 암소의 $25^{\circ}C$에서 저장기간(0, 12, 24, 48, 72, 144시간) 별로 시료를 채취해 TBARS와 PV 및 화학발광도를 측정하여 비교하였다. 저장시간에 따른 화학발광도 측정 결과는 특히 과산화물가 측정치와 유의적으로 높은 상관관계를 나타내었으며($r^2=0.987$), 상대적으로 높지는 않지만($r^2=0.874$) TBA가와도 유의적으로 상관관계가 있다고 조사되었다. 또한 실제 표준시료를 이용한 분석 결과에서도 유의적으로 높은 상관관계($r^2=0.9214$, 0.9599)를 나타내었다. 따라서 화학발광도를 이용한 지질 산패도 측정법은 여러 식품 등의 지질 산패도 측정에 있어서 POV를 대체할 수 있을 것으로 기대가 되며, 경우에 따라서는 TBARS에 대해서도 대체가 가능할 것으로 기대된다.

• 한국식품조리과학회지
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• 제27권6호
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• pp.793-801
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• 2011

We examined the quality characteristics of obtaining the optimal curing conditions for black garlic extract (BGE) added mackerel by using response surface methodology (RSM). The condition of RSM was included in 13 experimental points using two dependent variables: concentration of BGE (2.6~12.5 brix, X1) and curing time of BGE (17.58~102.42 min, X2). An assessment was conducted with four replicates on three independent variables: volatile basic amine (VBN, Y1), thiobarbituric acid (TBA, Y2), and shear force (Y3). The minimum content of VBN was 1.625 mg% at 3.17 brix, for 80.5 min. This was obtained when the BGE concentration was lower and the curing time was longer. The content of the TBA was 3.13~12.24 MA mg/kg at experiment range and the stationary point was at saddle point. Therefore, by using ridge analysis, the minimum point of TBA was 2.414 MA mg/kg at 3.5 brix for 84.4 min. The truth coefficient of the BGE concentration and curing time were 2.9 brix and 75.1 min, respectively, with a shear force ($120.764cm/kg^2$) shown at maximum point. The optimum Hunter color range was estimated from the lowest TBA value. The optimum range of the L color was 39.16~40.26, a and b color were 4.40~4.88 and 12.35~13.20, respectively.

• 한국식품과학회지
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• 제28권6호
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• pp.1157-1163
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• 1996

방아의 ether추출물을 중성, 페놀성, 산성 및 염기성 획분으로 분획한 다음 그들의 항산화 활성을 수소 공여능, 과산화물가, TBA가 및 흰쥐 간 microsome을 이용한 비효소적 지질 과산화 억제 활성 등으로 측정하고, 항산화 활성이 비교적 높게 나타나는 페놀성 획분과 중성 획분을 다시 소획분으로 분획한 다음 그중에서 항산화 활성이 강한 소획분성분을 GC/MS 및 NMR로 분석, 동정하였다. 페놀성 획분은 DPPH에 의한 수소 공여능, POV, TBA가 및 흰쥐 간 microsome의 지질 과산화 억제 활성 등 모두에서 높게 나타났으며, 중성 획분은 흰쥐 간 microsome의 지질 과산화 억제 활성에서 높게 나타났다. 페놀성 획분을 소획분으로 분획하였을 때 소획분 P-1, P-2 및 P-3에서 항산화 활성이 강하였으며, 특히 P-2는 합성 항산화제인 BHT나 천연 항산화물질로 알려져 있는 caffeic acid보다도 강하였다. 소획분 P-2를 TMS 유도체로 만들어 GC로 분석하였을 때 major peak 성분인 Peak I, II, III 및 IV 모두 TMS m/z 73를 base peak로 가지고 있어 4개의 peak 모두 -OH, -COOH기를 가지고 있는 것으로 확인할 수 있었다. 중성 획분의 소획분 중에는 N-2에서 강한 활성을 나타내었고 소획분 N-2의 주성분을GC/MS 및 NMR로 분석 한 결과 estragole로 확인되었다. Estragole의 흰쥐 간 microsome의 지질 과산화 50% 억제 농도는 $20{\sim}50\;{\mu}g/ml$로서 BHT보다는 약하였으니 caffeic acid, gallic acid와는 비슷한 수준의 항산화 활성을 나타내었다.