• Journal of Nutrition and Health
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• 제38권7호
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• pp.495-502
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• 2005

This study was performed to investigate effects of dietary folic acid supplementation on plasma homocysteine levels, thiobarbituric acid reactive substance s (TBARS) level s and liver SAM/SAH ratio in hyperhomocysteinaemia-induced pregnant rats. Forty-two female Sprague-Dawley rats were divided three groups (C: control diet, HFD: $0.3\%$ homocystine and 0 mg folic acid diet, HFS: $0.3\%$ homocystine and 8 mg/kg folic acid diet) according to homocystine and folic acid levels in the diet. They were fed experimental diets for 5 weeks prior to the mating and also during the entire period of pregnancy till gestational day 20. Dietary folic acid supplementation caused a significant decrease in plasma homocysteine levels which had been increased by a homocystine-diet, with a concomitant increase in plasma and liver folate levels. Liver TBARS levels in homocysteine-folic acid-deficient group (HFD) were higher than those in control group. Dietary folic acid supplementation increased hepatic SAM/SAM ratio in homocysteine-folic acid- sopplemetantion group (HFS) when compared to the HFD (p < 0.05). These data suggest that folate depletion and elevated plasma homocysteine may promote oxidative stress in rat livers and influence the remethylation cycle of the homocysteine metabolism detrimentally. In conclusion, dietary folic acid supplementation was found to be effective for lowering plasma homocysteine levels, relieving oxidative stress, and improving the methylation status in the body.

• 한국식품영양학회지
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• 제14권1호
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• pp.59-64
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• 2001

돼지고기 등심의 표면에 Lactococcus lactis subsp. Jactis ATCC l1454 유산균 배양액 3.96 log units의 처리구는 4$^{\circ}C$ 저장 1일 이후 대조구의 TBA가와 유의적 차이가 없었다 (P>0.05). 유산균 배양액 3.96 log units 처리구의 Hunter color L*과 b*가는 저장 동안 대조구와 유의적 차이가 없었다. 그러나 유산균 배양액 4.10과 4.23 log units 처리구의 Hunter a*가는 4$^{\circ}C$ 저장 1일과 6일 이후 대조구와 유의적 차이 (P<0.05)를 나타내었다. 유산균 배양액 4.10과 4.23 log units 처리구의 육즙유출율은 4$^{\circ}C$ 저장 4일 이후부터 처리구와 대조구 사이에 유의적 차이를 나타내었다.

• Journal of Pharmaceutical Investigation
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• 제1권1호
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• pp.22-29
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• 1971

Barbituric acid 및 thiobarbituric acid 유도체와 beta-cyclodextrin과의 상호반응(相互反應)을 수용액(水溶液)과 유기용매중(有機溶媒中)에서 각(各) 약물(藥物)을 용해도(溶解度), 분자용(分子容), 열역학적(熱力學的)인 면(面)에서 분석(分析) 검계(檢計)하였다. 본실험(本實驗)에 사용한 약물(藥物)이 모두 용액(溶液)에서는 beta-cyclodextrin과 반응(反應)하여 용해도(溶解度)가 증가(增加)하였으며 고형(固型)의 침전을 형성(形成)하지는 않았으나 비교적(比較的) 안정(安定)한 회합(會合)이 이루어졌다. 본반응(本反應)의 기전(機轉)은 주(主)로 beta-cyclodextrin의 주요(主要)한 성질(性質)의 하나인 inclusion능(能)에 의(依)하여 이루어 졌음을 보고(報告)한다.

• 한국식품위생안전성학회지
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• 제9권4호
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• pp.213-220
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• 1994

Customary usage of oil at homes and rancidity of edible soybean oil by cooking frequency at homes and mass meal services were investigated. 80% of house wives bought the cooking oil by 1.8ι unit container and 70% of them read either the manufactured date or explanatory note for use. 85% of house wives kept oil in the storage case under sink or in the pantry chest, and 80% of oils were used once or twice and 20% used three times for cooking. Acid value(AV), iodine value(IV), peroxide value(POV), carbonyl value(CoV) and thiobarbituric acid value(TBAV) of fresh soybean oil were lower than standard level. In the rancidity by cooking frequency, the acid value, peroxide value, carbonyl value and thiobarbituric acid value increased significantly when oil was used once and iodine value decreased significantly when used once and twice at both mass meal services and homes. The level of the acid value, iodine value, carbonyl value and thiobarbituric acid value of oil used at mass meal services did not show significant difference from those of oil used at homes. But, the peroxide value of oil used thrice at mass meal services was significantly higher than those of homes.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.510-512
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• 2010

A series of thiobarbituric acid derivatives were constructed and evaluated for inhibitory activity on hepatitis C virus NS5B polymerase. In biochemical assays using purified viral polymerase and RNA template, the $IC_{50}$ value was improved to 0.41 ${\mu}M$ from the original compound's 1.7 ${\mu}M$ value. In HCV sub genomic replicon assay, the $EC_{50}$ value was improved to 3.7 ${\mu}M$ from the original compound's 12.3 ${\mu}M$ value. $CC_{50}$ was higher than 77 ${\mu}M$ for all compounds tested, suggesting that they are useful candidates for anti-HCV therapy.

• Tuberculosis and Respiratory Diseases
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• 제44권1호
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• pp.104-113
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• 1997

연구배경 : 호기성 생물에서 산소의 대사과정 중에 산소의 불안전한 환원으로 산소유리기가 생성되는데 이들 산소유리기의 현저한 증가시 페손상 등 임상적으로 중요한 독성을 일으킬 수 있다고 5 알려져 있어 증가된 산화물이 여러 형태로 만성폐쇄성 폐질환의 발생에 관여 할 것으로 생각된다. 산소유리기의 폐손상과 이에 대한 항산화효소의 방어효과 및 활성도 변화를 관찰함으로 만성폐쇄성 폐질환의 병태생리의 일부분을 알 수 있겠다. 방법 : 만성폐쇄성 폐질환 환자군과 정상대조군 각 15명의 혈청과 적혈구에서 thiobarbituric acid reactant 변화와 항산화효소들(superoxide dismutase, glutathione peroxidase, catalase)의 활성도, 그리고 glutathione의 sulfhydry1기 를 측정하여 비교하였다. 결과 : Thiobarbituric acid reactant는 만성폐쇄성 폐질환 환자군에서 정상대조군보다 혈청과 적혈구에서 모두 유의한 증가를 보였고, superoxide dismutase활성도는 두 군사이에 유의한 차이가 없었으나, glutathione peroxidase와 catalase활성도는 만성폐쇄성 폐질환군에서 정상대조군보다 유의하게 감소되었다. 그리고 총 sulfhydryl기와 비단백 sulfhydryl기 모두 혈청과 적혈구에서 유의한 차이가 없었다. 결론 : 만성폐쇄성 폐 질환 환자에서 thiobarbituric acid reaclant의 증가를 보인 것은 산소유리기에 의한 세포손상을 나타내며, 항산화효소들중 superoxide dismutase는 큰 차이가 없었으나 glutathione peroxidase, catalase등은 대조군에 비해 유의하게 감소하여 만성폐쇄성 폐질환 환자에서 glutathione peroxidase 와 catalase 감소가 세포손상 기전의 한부분으로 작용한 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3382-3388
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• 2011

Reaction of barbituric acid (BA), 1,3-dimethyl barbituric acid (DMBA) and 2-thiobarbituric acid (TBA) with cyanogen bromide and aldehydes in the presence of L-(+)-tartaric acid afforded a new route for the synthesis of stable heterocyclic 5-aryl-1H,1'H-spiro[furo[2,3-d]pyrimidine-6,5'-pyrimidine]2,2',4,4',6'(3H,3'H,5H)-pentaones which is a dimeric form of barbiturate (uracil and thiouracil derivative). In the reaction of 1,3-diethyl thiobarbituric acid (DETBA) the Knoevenagel condensation and then Michael adducts were obtained under the same condition. Structure elucidation is carried out by $^1H$ NMR, $^{13}C$ NMR, FT-IR and Mass analyses. Mechanism of the formation is discussed.

• Bulletin of the Korean Chemical Society
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• 제28권11호
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• pp.1917-1918
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• 2007

• 한국가금학회지
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• 제21권4호
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• pp.249-256
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• 1994

This study was conducted to investigate the effects of the addition of varied levels of NaCI and phosphates to the breast meat of spent layers(2 \pm 0.2 kg), which were stabilized for over 24 h before slaughter, on the protein extractability, thiobarbituric acid(TBA) and volatile basic nitrogen (VBN). Within 1 h after slaughter, breast meat was removed and treated with NaCI(0, 1, 2, 3%) and phosphates(0.25% and 0.5%) using a hot-salted method. The breast meat samples were stored at 4\pm$1^{\circ}C$ for 3 d. The results obtained were summarized as follows. 1. Soluble protein contents of salt-treated groups were significantly higher than that of control (P<0.05) and showed a positive relationship with the levels of salt. At a constant level of NaCI, the soluble protein content was proportionately elevated by the levels of phosphates (P<0.05). It decreased significantly in both control and salt-treated groups during storage (P<0.05). 2. TBA values of salt-treated groups were significantly higher than that of control(P<0.05) and showed a positive relationship with the levels of salt. At a constant level of NaCI, TBA values in 0.5% phosphates treatment groups were significantly lower than that in 0.25%(P<0.05). It increased significantly in both control and salt-treated groups during storage(P<0.05). 3. VBN values of salt-treated groups were significantly lower than that of control(P<0.05) They increased significantly by the salt treatment for the first day of storage(P<0.05), but not from the second day of storage. VBN values in both control and salt-treated groups were significantly increased during storage(P<0.05). After the first day of storage and at the same level of NaCI, no significant difference in VBN value was observed between the two levels of phosphates.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.57-62
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• 1998

We hypothesized the primary effect of ginseng was to protect cell membrane fatty acids from decomposition caused by free radicals. To confirm the antioxidant effect of ginseng, we measured the inhibitory effect on the formation of thiobarbituric acid-reactive substances, an indicator of lipid peroxidation, and evaluated the free radical scavenging effect of ginseng by electron spin resonance spectrometer, and gas chromatography. The results showed that thiobarbituric acid-reactive substances formed and the loss of arachidonic acid during lipid peroxidation, and that hydroxyl (-like) radical peak formed by the iron complex (ferric nitrilotriacetate, an known free radical generator in vitro) were completely inhibited by ginseng extract. This antioxidant effect of ginseng may be responsible for its wide pharmacological actions in clinical practice. As the free radical reactions in general are rapid and non-specific, ginseng seems to act as a normalizer, rather than a general tonic, at the stages of acute or chronic active phase of the various diseases.