• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1170-1178
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• 2023

Food allergy represents a severe problem for many societies, including sensitive populations, academies, health authorities, and the food industry. Peanut allergy occupies a special place in the food allergy spectrum. To prevent consumption by consumers suffering from a peanut allergy, a rapid and sensitive detection method is essential to identify unintended peanut adulteration in processed foods. In this study, we produced four monoclonal antibodies (MAbs; RO 3A1-12, PB 4C12-10, PB 5F9-23, and PB 6G4-30) specific to thermo-stable and soluble proteins (TSSPs) of peanut and developed an enzyme-linked immunosorbent assay (ELISA) based on the MAbs. Among them, PB 5F9-23 MAb was firmly bound to Ara h 1, and other MAbs strongly reacted to Ara h 3 in the Western blot analysis. An antibody cocktail solution of the MAbs was used to enhance the sensitivity of an indirect ELISA, and the limit of detection of the indirect ELISA based on the antibody cocktail solution was 1 ng/ml and improved compared to the indirect ELISA based on the single MAb (11 ng/ml). The cross-reaction analysis revealed the high specificity of developed MAbs to peanut TSSPs without cross-reaction to other food allergens, including nuts. Subsequently, analyzing processed foods by indirect ELISA, all foods labeled as containing peanuts in the product description were confirmed to be positive. The results indicate that the developed antibodies exhibit high specificity and sensitivity to peanuts and can be used as bio-receptors in immunoassays or biosensors to detect intentional or unintentional adulteration of peanuts in processed foods, particularly heat-processed foods.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 추계 학술대회지
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• pp.12-27
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• 2000

During the recent decades, he problem of climate variability and change has been in the forefront of scientific problems. The objective of this study was to assess the impact of climate variability on crop growth and yield. The growth duration was the main impact of climate variability on crop yield. Phyllochronterval was shortened in the global worming situations. A simple model to describe developmental traits was provided from heading data of directly seeded rice cultivars and temperature data. Daily mean development rate could be explained by the average temperature during the growth stage. Simple regression equation between daily mean development rate(x) and the average temperature(y) during the growth period as y = ax + b. It can be simply modified as x = 1/a $\ast$ (y-b). The parameters of the model could depict the thermo sensitivity of the cultivars. On the base of this model, the three doubled CO2 GCM scenarios were assessed. The average of these would suggest a decline in rice production of about 11% if we maintained the current cultivars. Future cultivar's developmental traits could be suggested by the two model parameters.

• Korean Chemical Engineering Research
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• 제55권3호
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• pp.287-295
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• 2017

Using nanotechnology, magnetic nanoparticles of iron oxide were produced via co-precipitation method and followed modification with organic compounds. In the next step, functionalized monomer was provided via coupling ${\beta}$-cyclodextrine and allylamine onto modified magnetic nanoparticles. These nanoparticles were used to establish the adsorption rate of celecoxib. Magnetic nanoparticles are modified by (3-mercaptopropyl)trimethoxysilane. Nano-adsorbent was characterized by analytical and spectroscopic methods, such as Fourier transform infrared spectroscopy, elemental analysis, thermo-gravimetric analysis, and transmission electron microscopy (TEM). Laboratory parameters, such as the kinetics of adsorption isotherms, pH, reaction temperature and capacity were optimized. Finally, by using this nano-adsorbent in the optimized condition, extraction of celecoxib from biological samples as urine, drug matrix and blood plasma was carried out by high performance liquid chromatography with sensitivity and high accuracy.

• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.975-978
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• 2012

P63 is a gene product required in cell cycle regulation which plays vital roles in tumor differentiation. Aims of the present study were to assess the frequency, pattern, sensitivity and specificity of two p63 protein clones P63 4A4 and P63 4A4+Y4A3 in squamous cell carcinomas (SCCs). Thirty cases of head and neck region SCC diagnosed on the basis of H&E staining were examined along with 60 cases of head and neck region biopsies other than squamous cell carcinoma, negative on H&E staining, were taken as control. Immunostaining was performed on slides according to the Thermo Scientific UltraVision LP detection System. P63 4A4+Y4A3 clone is more sensitive 96.6% in comparison to 86% in P63 4A4 with having greater NPV of 98.3%. The results signify the importance of P63 4A4+Y4A3 marker over the old markers and may be used as a confirmatory marker of squamous cell carcinoma.

• Macromolecular Research
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• 제13권1호
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• pp.54-61
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• 2005

We prepared temperature-sensitive liposomes (TS-liposomes) modified with a thermo sensitive polymer, such as poly(N-isopropylacrylamide) (PNIPAAm), to increase the degree of drug release from liposomes at the hyperthermic temperature. A PNIPAAm hydrogel containing TS-Iiposomes was also prepared to obtain a hydrogel complex at body temperature. In addition, a depot system for local drug delivery using the polymer hydrogel was developed to enhance therapeutic efficacy and prevent severe side effects in the whole body. The PNIPAAm-mod­ified TS-liposome was fixed into the PNIPAAm hydrogel having a high temperature-sensitivity. The release behavior of calcein, a model drug, from TS-liposomes in the PNIPAAm hydrogel was then initiated by external hyperthermia; the results indicated that sustained release as a function of temperature and time was caused by the thermosensitivity of the liposome surface and diffusion of the drug into the PNIPAAm hydrogel. Our results indicated that TS-liposomes in a PNIPAAm hydrogel represented a plausible system for local drug delivery.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2010년도 제35회 추계학술대회논문집
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• pp.321-327
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• 2010

마이크로 크기의 단일 알루미늄 해석을 위한 간단한 모델을 작성하고, 현상의 주요 파라메터를 도출하는 연구를 수행하였다. 금속 입자의 연소는 점화와 준정상상태의 연소 단계로 구성하였고, 각 단계는 액적 연소의 경우와 유사하게 보존 및 이송 방정식들을 사용하여 모사되었다. 모델은 기존의 실험 데이터와의 엄격한 비교를 통해 신뢰성을 검증하였고, 이 과정에서 현상의 주요 변수를 도출하여 그 영향을 평가하였다. 주요 변수로는 초기 입자크기, 산화 피막 두께, 대류 열전달의 유무, 외기온도, 압력 등이 선정되었고, 간단한 열역학적 모델임에도 불구하고 정량적으로 실험 데이터와 유사하게 각각의 파라메터의 영향을 평가할 수 있음을 확인하였다.

• 대한기계학회논문집B
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• 제33권8호
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• pp.573-579
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• 2009

A thermal mass air flow sensor, which consists of a micro-heater and thermal sensors on the silicon-nitride thin membrane structure, is micro-fabricated by MEMS processes. Three thermo-resistive sensors, one for the measurement of microheater temperature, the others for the measurement of membrane temperature upstream and downstream of the micro-heater respectively, are used. The micro-heater is operated under the constant temperature difference mode via a real time controller, based on inlet air temperature. Two design models for microfabricated flow sensor are compared with experimental results and confirmed their applicabilities and limitations. The thermal characteristics are measured to find the best flow indicator. It is found that two normalized temperature indicators can be adopted with some advantages in practice. The flow sensor with this control mode can be adopted for wide capability of high speed and sensitivity in the very low and medium velocity ranges.

• Bulletin of the Korean Chemical Society
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• 제25권9호
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• pp.1361-1365
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• 2004

Solvatokinetic and solvatochromic behavior of bis(indolinobenzospiropyranyl) sulfide derivatives 1a-1c have been studied in various solvents. The marked negative solvatochromism is exhibited for 1a and 1b in the whole region of solvent polarity examined. Whereas, it is found only in the polar solvent region ($E_T$ > 37) for 1c. The sensitivity order to the solvent media (slope values) is 1a > 1b > 1c. The branched linear plot with a zero slope was shown for the most sterically-hindered compound 1c in the less polar-solvent region (($E_T$ < 37). The biphasic plot is indicative of dual mechanistic process, i.e., a transition state with increased zwitter-ionic character in more polar solvents and electrocyclic process with an isopolar transition state in less polar solvents.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2011년도 제36회 춘계학술대회논문집
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• pp.341-350
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• 2011

마이크로 크기의 단일 마그네슘 입자의 연소 해석을 위해 간단하면서도 점화와 연소과정 전체를 모사한 모델을 사용하여 각각의 주요 파라메터별 영향을 도출하는 연구를 수행하였다. 계산에 사용된 모델은 액적 연소의 경우와 유사하게 보존 및 이송 방정식들을 사용하여 모사되었으며 입자의 온도가 1200 K에 도달하면 점화단계를 종료하고 준정상연소 단계로 전환되었다. 선행 연구를 참고하여 주요 파라메터를 선정하였으며 주요 파라메터로는 초기 입자크기, 대류 열전달의 유무, 외기 온도, 압력 등이 선정되었고, 간단한 열역학적 모델임에도 불구하고 정량적으로 실험 데이터와 유사하게 각각의 파라메터의 영향을 평가할 수 있음을 확인하였다.

• Nuclear Engineering and Technology
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• 제53권3호
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• pp.752-762
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• 2021

The Smoothed Particle Hydrodynamics is one of the most widely used mesh-free numerical method for thermo-fluid dynamics. Due to its Lagrangian nature and simplicity, it is recently gaining popularity in simulating complex physics with large deformations. In this study, the 3D single/two-phase numerical simulations are performed on the Liquid Metal Reactor (LMR) centralized sloshing benchmark experiment using the SPH parallelized using a GPU. In order to capture multi-phase flows with a large density ratio more effectively, the original SPH density and continuity equations are re-formulated in terms of the normalized-density. Based upon this approach, maximum sloshing height and arrival time in various experimental cases are calculated by using both single-phase and multi-phase SPH framework and the results are compared with the benchmark results. Overall, the results of SPH simulations show excellent agreement with all the benchmark experiments both in qualitative and quantitative manners. According to the sensitivity study of the particle-size, the prediction accuracy is gradually increasing with decreasing the particle-size leading to a higher resolution. In addition, it is found that the multi-phase SPH model considering both liquid and air provides a better prediction on the experimental results and the reality.