• 한국식품영양학회지
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• 제12권3호
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• pp.265-270
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• 1999

The stabilization of Aspergillus sp. $\alpha$-amylase was attained by modification with periodate-oxidized sol-uble starch. The pH stability of modified enzyme was increased at pH 3~4 and 9~11 in the presence of $\alpha$-cyclodextrin($\alpha$-CD) compared with that of native enzyme. Thermal stability of the modified enzyme was increased. After treatment at 6$0^{\circ}C$ for 30min the activity remained 20% for the enzyme modified at pH 9.7 in the presence of $\alpha$-CD and tested in the presence of $\alpha$-CD 10% for the enzyme modified at pH 9.7 in the presence of $\alpha$-CD 0% for the native enzyme. The native enzyme and modified enzyme showed one peak in HPLC. The substrate specificity of the modified enzyme was not changed in HPLC analysis of reaction product.

• 생명과학회지
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• 제11권3호
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• pp.230-234
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• 2001

The cyclodextrin glucanotransferase(CGTase) gene of Bacillus macerans was expressed in Saccharomyces cerevisiae and the recombinant CGTase was partially purified from the yeast culture supernatant. The optimal pH and temperature of the CGTase were found to be 6.0 and 5$0^{\circ}C$, respectively. The pH and temperature stabilities of the recombinant enzyme were significantly enhanced and the half life at 55$^{\circ}C$ was about 60 hr. When the recombinant CGTase was reacted with 5% soluble starch, the conversion yield of total cyclodextrin (CD) from starch was estimated to be 41% at 48 hr, whereas the wild type enzyme showed the yield of 12%. This improvement of conversion yield and thermal stability of CGTase may be useful for the development of low-cost CD production process.

• Journal of Magnetics
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• 제1권1호
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• pp.51-54
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• 1996

The effects of additional P-doping on the magnetic properties, thermal stability and cation distribution of Co-doped ${\gamma}-{Fe_2} {O_3}$have been investigated by means of magnetic annealing and measurements with vibration sample magnetometer and torque magnetometer. It is found that the P-doping promotes the coercivity and its magnetic-thermal stability, which may be attributed to increase of the cubic magneto-crystalline anisotropy constant, $K_1$ and the activation energy, E, for cation rearrangement, respectively. The cation distribution of P and Co-substituted iron oxide was calculated from the variation of the saturation magnetization with P-doping on the basis of the Neel model. It was found that the most of P ions in the iron oxides occupied the B-site of spinel lattice.

• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1521-1530
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• 2023

An α-L-rhamnosidase gene from Thermoclostridium. stercorarium subsp. thermolacticum DSM 2910 (TstRhaA) was cloned and expressed. The maximum TstRhaA activity of the protein reached 25.2 U/ml, and the molecular mass was approximately 106.6 kDa. The protein was purified 8.0-fold by Ni-TED affinity with an overall recovery of 16.6% and a specific activity of 187.9 U/mg. TstRhaA activity was the highest at 65℃ and pH 6.5. In addition, it exhibited excellent thermal stability, better pH stability, good tolerance to low concentrations of organic reagents, and high catalytic activity for p-nitrophenyl-α-L-rhamnopyranoside (pNPR). Substrate specificity studies showed that TstRhaA exhibited a high specific activity for rutin. At 60℃, pH 6.5, and 0.3 U/ml enzyme dosage, 60 g/l rutin was converted to 45.55 g/l isoquercitrin within 150 min. The molar conversion rate of rutin and the yield of isoquercitrin were 99.8% and 12.22 g/l/h, respectively. The results suggested that TstRhaA could be used for mass production of isoquercitrin.

• 한국식품과학회지
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• 제23권4호
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• pp.442-446
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• 1991

Fuji 사과로부터 acetone 처리 및 CM-cellulose column chromatography를 통하여 3개의 peroxidase isozyme(peak I, II, III)을 분리하고 isoelectric focusing에 의하여 pI값이 각각 3.80, 8.82, 8.35임을 확인하였다. Peroxidase isozyme의 최적 pH는 guaiacol과 $H_{2}O_{2}$를 기질로 하였을 때 peak I이 pH5.0, peak II, III은 pH5.5 이었으며 최적온도는 peak I, II, III 모두 $40^{\circ}C$이었다. $70^{\circ}C$에서 각 isozyme의 D값은 660초, 1,320초, 600초로서 peak II isozyme이 가장 열에 안정하였다. 당(0.032 M)의 존재하에서는 sucrose와 lactose는 효소의 열안정성에 영향을 미치지 않았으나 fructose와 glucose는 열안정성을 현저하게 감소시켰다. 이는 환원당과 효소단백질의 상호작용에 기인한 것으로 생각된다.

• BMB Reports
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• 제35권5호
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• pp.472-475
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• 2002

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lectins that possess canonical dimers, thermal denaturation studies show that the refolding of phytohemagglutinin L at neutral pH is irreversible. Differential scanning calorimetry (DSC) was used to study the denaturation of this lectin as a function of pH that ranged from 2.0 to 3.0. The lectin was found to be extremely thermostable with a transition temperature around $82^{\circ}C$ and above $100^{\circ}C$ at pH 2.5 and 7.2, respectively. The ratio of calorimetric to vant Hoff enthalpy could not be calculated because of its irreversible-folding behavior. However, from the DSC data, it was discovered that the protein remains in its compact-folded state, even at pH 2.3, with the onset of denaturation occurring at $60^{\circ}C$.

• Applied Biological Chemistry
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• 제15권3호
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• pp.207-211
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• 1972

1. 고온성 사상균인 Myriococcum albomyces의 밀기울 액체배양액에는 xylanase 와 laminaranase활성이 있었다. 2. Xylanase와 laminaranase의 최적 pH는 각각 5.0과 6.0이었다. 그리고 xylanase의 pH안 전범위는 $pH\;4.0{\sim}pH\;7.0$ 이었다. 3. Xylanase의 최적온도는 $55^{\cicr}C$이고 laminaranase의 척적온도는 $65^{\cicr}C$ 였다. 4. Xylanase의 열안전성은 $65^{\cicr}C$65에서 55분간, laminaranase 는 70 분간 안전하였다.

• 한국식품영양학회지
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• 제18권1호
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• pp.4-10
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• 2005

과요오드산-산화가용성전분은 Aspergillus awamori a-glucosidase의 pH 안정성을 증가시켰다. 40℃에서 두시간 항온시킨 결과, 과요오드산-산화가용성전분이 존재하지 않을 때의 효소는 pH 3∼7, 존재할 때의 효소는 pH 3∼9, 50℃에서 과요오드산-산화가용성전분이 존재하지 않을 때의 효소는 pH 3∼6, 존재할 때의 효소는 pH 3∼8 범위에서 안정하였다. 60℃에서는 과요오드산-산화가용성전분의 존재여부에 관계없이 효소는 pH 3∼6 범위에서 안정하였으나 pH 5와 6에서 과요오드산-산화가용성전분이 존재하면 효소의 잔존활성은 존재하지 않을 때보다 20% 더 높았다. 과요오드산으로 변형한 효소는 pH 9에서 활성이 70% 남았으나 변형하지 않은 효소는 남지 않아서 변형으로 안정성이 증가된 것으로 나타났다. 변형효소는 50℃에서 12%, 80℃에서 7%의 활성이 남았으나 변형시키지 않은 효소는 50℃에서 8%가 남고, 70℃이상에서는 남지 않았다. HPLC 분석 결과 pH 2 이하 및 9 이상에서는 효소의 서브유니트가 분리되고, 변성 중합되었다. 변형하지 않은 효소는 산성과 알칼리성 pH에서 변성되어 단백질의 구조가 무너졌지만 과요오드산-산화가용성전분이 존재하면 변성되지 않았다.

• 한국세라믹학회지
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• 제32권1호
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• pp.63-70
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• 1995

A crack-free ceramic composite membrane with micropores has been synthesized by the pressurized sol-gel coating technique using the mixed Al2O3-SiO2 sols. The mixed sols were prepared by mixing nanoparticulate SiO2 and boehmite sols. These sols were more stable at lower pH, but very unstable when their copositions were in the range of 50~75mol% of SiO2 at the same pH. The mixed Al2O3-SiO2 membrane prepared from the mixed sol (0.2mol/$\ell$ of solid content and pH=2) containing 40mol% of SiO2 had the mean pore radius of 0.80nm and the specific surface area of 280$m^2$/g. The nitrogen permeability through the coated Al2O3-SiO2 layer was 42$\times$107mol/$m^2$.s.Pa. It was found that the thermal stability of aluminosilicate membranes, even through similar to that of SiO2 membranes, was much improved in comparison with ${\gamma}$-alumina membranes.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2004년도 Proceedings of ISRS 2004
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• pp.452-455
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• 2004

MSC (Multi-Spectral Camera) system is a remote sensing instrument to obtain high resolution ground image. EOS (Electro-Optic System) for MSC mainly consists of PMA (Primary Mirror Assembly), SMA (Secondary Mirror Assembly), HSTS (High Stability Telescope Structure) and DFPA (Detector Focal Plane Assembly). High performance of EOS makes it possible for MSC system to provide high resolution and high quality ground images. Temperature of the EOS needs to be controlled to be in a specific range in order not to have any thermal distortion which can cause performance degradation. It is controlled by full redundant CPU based electronics. The validity of thermistor readings can be checked because a few thermistors are installed on each control point on EOS. Various kinds of thermal control logics are used to prevent 'Single Point Failure'. Control logic has a few set of database in order not to be corrupted by SEU (Single Event Upset). Even though the thermal control logic is working automatically, it can also be monitored and controlled by ground-station operator. In this paper, various ways of thermal control logic for EOS in MSC will be presented, which include thermal control mode and logic, redundancy design and status monitoring and reporting scheme.