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Exo-O-Glycosylhydrolases in Korea Ginseng Roots

  • Yelena V.Sundukova;Lee, Mi-Ja;Park, Hoon
    • Journal of Ginseng Research
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    • v.24 no.2
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    • pp.89-93
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    • 2000
  • WB were screening the stele and the cortex of the ginseng roots (Panax ginseng C.A.Meyer) on the exo-0-glycosylhydrolase activities during vegetation period of 1999 year. The following p-nitrophenylglycosides were used to test exe-0-glycosylhydrolase activities: $\alpha$- and $\beta$-D-galactopyranosides,$\alpha$- and $\beta$-D-glucopyranosides, $\alpha$- and $\beta$-D-mannopyranosides, N-acetyl-$\beta$-D-glucosaminide, $\alpha$- and $\beta$-D-xylopyranosides $\alpha$- L-rhamnopyranoside, $\beta$-D-glucuronide, $\beta$-D-galacturonide, $\beta$-L-,$\alpha$-L- and $\beta$-D-fucopyranosides, $\alpha$-L-arabinopyranoside. Only $\beta$-D-galactosidase, $\alpha$-L-mannosi-dase , N- acetyl- ${\beta}$-D-slucosarninidase, $\alpha$-D-galacto sidase, $\alpha$-L-arabinosidase, and $\beta$-D-fuco sidase were found in both partsof ginseng roots. Their contents during the vegetation period were shown to differ considerably, being dependent not only on plant development stage but on plant tissue and environmental conditions too.

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A Study on Effect of Situation Coping Training Program for international Math Olympiad Candidates (국제 수학 올림피아드 참가 후보자들을 위한 상황대처훈련에 관한 연구)

  • 김보경;조성희;이군현
    • Journal of Gifted/Talented Education
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    • v.5 no.2
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    • pp.55-89
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    • 1995
  • Currently Korea encourages gifted highschoolers and junior high schoolers to participate in international achievement contests such as International Olympiads. Participants for these contests are selected nationwide among gifted students in areas of mathematics, physics, chemistry, and others. They go through a series of screening tests and programs. One of the screening processes is Korean Olympiad School, which provides study programs each summer for student-candidates prior to following year's International Olympiads. Approximately 40 students of high schools and junior high schools, in each subject of study, gather at Korean Olympiad Summer School, and they go through intensive study programs during short period of time. Out of 40 candidates, less than 20 students are finally selected to participate in International Olympiads. In this study, a psycho-educational program called "Situatin Coping Training Program" was developed to enhance ahievement motivation for these student-candidates. This study was to see if this tranining program actually improved their cognitive, emotive motivation factors, and to see how this training program affected their achievement level. Training was administered for five days. This training program was found effective for participants to increase self-efficacy, internal locus of control, and anxiety coping. These cognitive and emotive motivation factors, other than intelligence, were found to have positive relationship with achievement level, of which self-efficacy and attribution style of students were found as two best predictors of achievement. This training program was perceived as necessary by participants, and helpful for recovering self-confidence and self-control as well as coping pressure. Suggestions were made that this kind of training program be administered as regular curriculum in preparative study programs such as Korean Olympiads, since cognitive, emotive motivation factors are related with achievement, and furthermore, be utilized in all gifted education programs in Korea. in Korea.

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Irritant Contact Dermatitis in a Dog (개에서 발생한 자극성 접촉성 피부염 1례)

  • Kang, Jong-Il;Park, Jeong-Ho;Lee, Wang-Hee;Oh, Tae-Ho;Cho, Sung-Whan;Park, Seong-Jun
    • Journal of Veterinary Clinics
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    • v.28 no.6
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    • pp.607-609
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    • 2011
  • A 6-year-old, neutered female, Cocker spaniel presented with severe abdominal erythema and crusts. These conditions developed 1 month ago. This patient had a history of using humectant spray for several months. Irritant contact dermatitis was diagnosed by history, clinical signs, laboratory and histopathologic examinations (H-E stain). Complete blood count and serum chemistry showed no remarkable findings. Histopathologic examination of skin samples revealed parakeratosis accompanied by acanthosis of the dermis and mild perivascular inflammations of the superficial dermis. Clinical signs were improved after avoidance of suspected offending substance.

Long-term Management of Copper-associated Hepatic Cirrhosis with D-penicillamine, SAMe, and DBB in a Dog (개에서 발생한 구리 축적성 간경화에 D-penicillamine, SAMe, DBB로 병용 치료하여 장기간 생존한 1례)

  • Seo, Kyoung-Won;Lee, Young-Heun;Bhang, Dong-Ha;Ahn, Jin-Ok;Coh, Ye-Rin;Hwang, Cheol-Young;Kim, Dea-Yong;Youn, Hwa-Young
    • Journal of Veterinary Clinics
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    • v.28 no.2
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    • pp.249-253
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    • 2011
  • A 4-year-old intact female American Cocker Spaniel presented with lack of appetite, shivering, and abdominal distension. It was initially diagnosed with chronic hepatitis with cirrhosis, by serum chemistry, radiography, ultrasonography, and histopathologic examination following liver biopsy. Abundant copper granules were detected in most hepatocytes with rhodanine stain, with hepatic copper concentration at 1460 ppm (reference range: <400 ppm). Based on these findings, copper-associated hepatitis with cirrhosis was diagnosed and successfully managed with long-term D-penicillamine, s-adenosylmethionine, biphenyl-dimethyl-dicarboxylate and supportive care. The spaniel died 35 months after diagnosis.

Benign Symmetrical Lipomatosis (Madelung's Disease) -A case report- (양성 대칭성 지방종증 -1예 보고-)

  • Han Jong-Hee;Kang Min-Woong;Yu Jae-Hyeon;Lim Seung-Pyung;Lee Young;Na Myung-Hoon
    • Journal of Chest Surgery
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    • v.39 no.5 s.262
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    • pp.419-422
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    • 2006
  • Benign symmetric lipomatosis (BSL), also called Madelung's disease, is a rare disease in middle-aged chronic alcohol user. The cause of BSL is unknown. A 63 year-old man with rapid growing lesions in both shoulders for 2 months visited our hospital. Except for cosmetic problem, no abnormal finding was found in blood cell analysis and chemistry; however, excessive fat deposition was found on radiographic findings. Lipoma was revealed in pathologic examination and BSL was diagnosed clinically. Patient is being followed up without any specific problem.

A Case of Concurrent Gallbladder Mucocele and Hypothyroidism in a Dog; Dermatologic, Serum Chemical and Ultrasonographic Changes (담낭점액종과 갑상선기능저하증이 병발한 개에서 피부, 혈청화학치 및 초음파상의 변화)

  • Cho, Sun-Hyung;An, Daegi;Kim, Ha-Jung;Kang, Ji-Houn;Chang, Dong-Woo;Jung, Dong-In;Lim, Ha-Young;Yang, Mhan-Pyo;Kang, Byeong-Teck
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.464-467
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    • 2013
  • A 6-year-old, spayed female Cocker Spaniel was presented with generalized seborrhea sicca. Initially gallbladder mucocele (GM) was suggested by high liver enzyme activities and a stellate pattern in ultrasonography. There were no responses during 16 months of medical treatments for GM. Hormonal assay and histopathological findings were consistent with a diagnosis of hypothyroidism. Levothyroxine therapy led to rapid resolution of dermatologic, serum chemical and ultrasonographic abnormalities. This report describes serial changes of skin lesions, serum chemistry and ultrasonography in a dog with concurrent GM and hypothyroidism following treatment of hypothyroidism.

Synthesis of Artificial Zeolite from Fly Ash for Preparing Nursery Bed Soils and the Effects on the Growth of Chinese Cabbage (석탄회(石炭灰)를 이용한 육묘(育苗) 상토용(床土用) 인공(人工) 제올라이트의 제조와 배추 생육에 미치는 효과(效果))

  • Kim, Yong-Woong;Lee, Hyun-Hee;Yoon, Chung-Han;Shin, Bang-Sup;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.2
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    • pp.95-106
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    • 1998
  • To reduce the environmental contamination and to utilize fly ash massively produced from the coal power plant every year, we synthesized the artificial zeolite using fly ash treated with alkaline, and then analyzed the mineralogical and morphological properties by X-ray, IR, and SEM. The amount of $NH_4{^+}$, $K^+$, and $H_2PO_4{^-}$ adsorbed by the fly ash and the artificial zeolite were determined with reaction time, amount of adsorbate used, ion concentrations. The results obtained from the pot experiments packed with the top soil, amended with granulated artificial zeolite which was made by treatment of 4% polyvinylalcohol, showed that CEC of the artificial zeolite was $257.7cmol^+kg^{-1}$, that was almost 36 times greater than that of fly ash. The ratio of $SiO_2/Al_2O_3$ decreased but the amount of Na increased. The physico-chemical properties analyzed by X-ray, IT, and SEM represented that the artificial zeolite synthesized had a similar morphological structure to that of the natural zeolite. The structures of the artificial zeolite had a significantly enlarged surface having a lot of pores, while the fly ash looked like spherical smooth shape with having not pores on the surface. Thus, the artificial zeolite was successfully synthesized. The results of adsorption isotherms of fly ash and artificial zeolite showed that the amount of $NH_4{^+}$, $K^+$, and $H_2PO_4{^-}$ adsorbed increased as the equilibrium concentration increased, while $NH_4{^+}$ was strongly adsorbed on the surface of fly ash and artificial zeolite than that of $K^+$. The most distinctive growth of Chinese cabbage was found from the top soil(NPK + soils + 20% of granulated artificial zeolite + 5% of compost). Therefore, we concluded that one of the most effective methods to effectively recycle a fly ash was to make the artificial zeolite as we did in this experiment.

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Residue Studies of Difenoconazole and Thiamethoxam during Cultivation of Sweet Persimmon for Export (수출용 단감에 대한 Difenoconazole과 Thiamethoxam의 잔류특성 연구)

  • Chang, Hee-Ra;Kang, Hae-Rim;Do, Jung-A;Oh, Jae-Ho;Hwang, In-Kyun;Kwon, Ki-Sung;Im, Moo-Hyeog;Kim, Kyun
    • Korean Journal of Environmental Agriculture
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    • v.31 no.3
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    • pp.248-254
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    • 2012
  • BACKGROUND: In order to elucidate residual characteristics of difenoconazole and thiamethoxam by treatment to sweet persimmons for one year and to generate the data for the maximum residue limit (MRL) establishment for those pesticides in or on sweet persimmon. METHODS AND RESULTS: Systemic fungicide difenoconazole WP (10% a.i.) and systemic insecticide thiamethoxam WG (10% a.i.) were sprayed onto 12~25-years-old sweet persimmons according to its preharvest interval (PHI), respectively, and then fresh sweet persimmons were harvested at 0, 1, 3, 7, 14, 21 days after treatment from pesticide-sprayed plots at each 3 sites. The analytical methods were evaluated to limit of quantification, linearity, specificity, reproducibility and recoveries. The crop samples were extracted with acetone and performed dichloromethane partition process. The extracted samples of difenoconazole were analyzed by GC-ECD and the thiamethoxam extracted samples were analyzed by HPLC with good sensitivity and selectivity of the method. The average recoveries of difenoconazole ranged from 87.5 to 99.5% with the percentage of coefficient variation in the range 4.1~7.6% at three different spiking levels(0.02, 0.2 and 2.0 mg/kg). And the average recoveries of thiamethoxam and clothianidin ranged from 88.8 to 98.9% and 83.2 to 96.6% with the percentage of coefficient variation in the range 3.6~5.0% and 3.8~9.4% at three different spiking levels(0.02, 0.2 and 2.0 mg/kg), respectively. The residue amounts ranges of difenoconazole were 0.2~0.56 mg/kg and the residue amount was decreased below the MRL level, 1.0 mg/kg, after 1 day harvest. The residue amounts ranges of thiamethoxam were 0.08~0.28 mg/kg and the residue amount was decreased below the MRL level, 0.5 mg/kg, after 1 day harvest. And the residue amount of clothianidin was below then 0.03 mg/kg for only one test site of 14 and 28 day samples. CONCLUSION: As a result, the residual amounts of difenoconazole and thiamethoxam were not exceeded the MRL of established criteria for sweet persimmon. The biological half-lives of difenoconazole and thiamethoxam were 13.6, 19.4, 16.3 and 10.0, 15.3, 14.0 days at each three test sites, respectively.

Hepatic Cirrhosis Secondary to chronic Hepatitis in an English Cocker Spaniel (ECS) Dog (잉글리쉬 코커스파니엘 견에서 발생한 만성 간염 및 간경화 증례)

  • Park Chul;Yoo Jong-Hyun;Jung Dong-In;Kim Ha-Jung;Kang Byeong-Teck;Lim Chae-Young;Yoon Hun-Young;Jeong Soon-Wuk;Sur Jung-Hyang;Park Hee-Myung
    • Journal of Veterinary Clinics
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    • v.23 no.1
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    • pp.72-76
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    • 2006
  • A 1-year-old, female English cocker spaniel (ECS) dog was presented with 3-month history of vomiting and retaking of the vomitus, and chronic weight loss. The client had noticed mild abdominal distension 10 days before. The dog was diagnosed as chronic hepatitis with hepatic cirrhosis based on complete blood count (CBC), serum chemistry profiles, radiography, ascites assessment, bile acid evaluation, and liver biopsy through exploratory laparotomy and necropsy. CBC and serum chemistry profiles revealed mild anemia, slightly elevated hepatic enzymes (ALT and AST), increased creatinine kinase (CK), hyperammonemia, and hypoproteinemia with hypoalbuminemia. Ascites was transudate according to analysis of components. Bile acid assessment (fasting; $174.4{\mu}mol/L$ and postprandial; $198.4{\mu}mol/L$) showed strongly suspected hepatic insufficiency. On radiological findings, ascites was evident. Atrophied liver (especially left side lobes) and distended mesenteric vasculatures were observed by exploratory laparotomy. Histopathological examination of marginal lesion of left lateral lobe of liver by biopsy revealed the necrosis of hepatic cells, dilation of sinusoids, infiltration of neutrophils in sinusoids, and vacuolation of hepatic cytoplasm. The patient had been managed with careful low protein diet and specific supportive therapy (ursodeoxycholic acid, prednisolone, vitamine E, and interferon). Vomiting and ascites disappeared with medical management. The dog was monitored periodically by CBC, serum chemistry and radiographic examination. The dog survived more 18 months with medical therapy. After spontaneous death, necropsy and histopathologic examination were performed.

Heavy Metal and Amino Acid Contents of Soybean by Application of Sewage and Industrial Sludge (생활하수 및 산업폐수 슬러지 처리에 따른 콩의 중금속 및 아미노산 함량)

  • Moon, Kwang-Hyun;Kim, Jae-Young;Chang, Moon-Ik;Kim, Un-Sung;Kim, Seong-Jo;Baek, Seung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.2
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    • pp.268-277
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    • 2013
  • This study investigates the effects of accumulated levels of heavy metals and nutrients of cultivated soybean plant tissues, after the continuous application of sewage sludge (SS) and industrial sludge (IS). SS and IS were applied to soybean plants at loading of 0, 11.25, 22.50, and 45.00 Mg/ha, and the contents of heavy metals (Cd, Pb, Ni, Cu, and Zn), proteins, and amino acids in the cultivated soybean plants were measured. The Cd content in the soybean was 0.02~0.05 mg/kg, which is within the safety level set in the standard, and that of Pb was 0.02~0.15 mg/kg, which is also within the safety level except for IS 45 Mg/ha. The soybean harvest quantity was higher in the treatment groups than the control group in the first year. However, in the second year, SS had lower harvest and IS had the same level or a decreasing tendency, compared with the control group. In the first year, the content of amino acid which followed handling of SS was increased in the sludge groups more than in the control group in the case of glutamate. However, the influence of continuous application was increased in the sludge groups in the case of amino acids of 12 types. In conclusions, the accumulation in soybean of heavy metals by sludge treatment is not a problem, but the decreased yields needs to be considered. In addition, the most appropriate level of sludge treatment was 11.25 Mg/ha.