• BMB Reports
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• 제31권3호
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• pp.254-257
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• 1998

The gshI gene from the Escherichia coli K-12 strain codes for ${\gamma}-glutamylcysteine$ synthetase which mediates the rate-limiting step of glutathione biosynthesis. The isolated gshI gene from E. coli K-12 has an unusual translation initiation codon, UUG. The 494th amino acid is Ala rather than Gly which was found in a mutant strain E. coli B. In order to improve the translational rate of the gshI gene of E. coli K-12, the initiation codon, UUG, was changed to the usual AUG codon by the site-specific mutagenesis. This change has resulted in a 53% increase of ${\gamma}-glutamylcysteine$ synthetase activity. The enzyme activity was also improved by replacing $Ala^{494}$ with Val (A494V) or Leu (A494L). The replacement of $Ser^{495}$ with Thr (S495T) also resulted in a 62% increase of the enzyme activity. Therefore, the specific activity of ${\gamma}-glutamylcysteine$ synthetase was increased with the increasing chain length of the aliphathic amino acid at the site of the 494th amino acid (Ala<$Val{\leq}Leu$).

• 한국치위생학회지
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• 제16권3호
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• pp.417-426
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• 2016

Objectives: The objective of the study was to investigate the related factors of preventive behavior experience toward dental caries and periodontal disease in Korean adolescents. Methods: The study subjects were 72,060 adolescents in 800 schools who completed 2014 Korean Youth Risk Behavior Web-based survey. Dependent variables included general characteristics of the subjects, oral health behavior, and oral disease experience. The experience rate of sealant and scaling showed the prevention behavior of dental caries and periodontal disease. Data were analyzed by IBM SPSS statistics 20.0. Results: The experience rate of sealant accounted for 26.9%. The experience of sealant was related with gender, school, maternal education level, economic status, vigorous physical activity, smoking, usual stress, fruit consumption, milk consumption, soda consumption, snack consumption, tooth brushing, oral health education experience, periodontal bleeding and pain, mucosal disorders, and bad breath. The experience rate of scaling was 22.6%. The experience of scaling was related with gender, school, city division, learning achievement, maternal education level, economic status, residential type, vigorous physical activity, usual stress, fruit consumption, milk consumption, tooth brushing, oral health education experience, periodontal bleeding and pain, mucosal disorders, and bad breath. Conclusions: To expand preventive oral health behavior in the adolescents, it is necessary to support the systematic policy making and monetary establishment in the future.

• Food Science and Biotechnology
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• 제17권5호
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• pp.904-911
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• 2008

Covalent cross-links between a number of proteins and peptides explain why transglutaminase may be widely used by food processing industries. The objective of this work was optimization of the fermentation process to produce transglutaminase from a new microbial source, the Streptomyces sp. P20. The strategy adopted to modify the usual literature media was: (1) fractional factorial design (FFD) to elucidate the key medium ingredients, (2) central composite design (CCD) to optimise the concentration of the key components. Optimization of the medium resulted in not only an 86% increase in microbial transglutaminase activity as compared to the media cited in the literature, but also a reduction in the production cost. Optimal fermentation conditions - namely temperature and agitation rate - were also studied, using CCD methodology. Usual conditions of $30^{\circ}C$ and 100 rpm were within the optimal area. All other parameters for enzyme production were experimentally proven to be optimum fermentation conditions.

• 한국대기환경학회지
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• 제30권1호
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• pp.26-36
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• 2014

The uncertainty assessment is important to improve the reliability of emission inventory data. The DARS (Data Attribute Rating System) have recommended as the uncertainty assessment technic of emission inventory by U.S. EPA (Environmental Protection Agency) EIIP (Emission Inventory Improvement Program). The DARS score is based on the perceived quality of the emission factor and activity data. Scores are assigned to four attributes; measurement/method, source specificity, spatial congruity and temporal congruity. The resulting emission factor and activity rate scores are combined to arrive at an overall confidence rating for the inventory. So DARS is believed to be a useful tool and may provide more information about inventories than the usual qualitative grading procedures (e.g. A through E). In this study, the uncertainty assessment for 2009 CAPSS (Clean Air Policy Support System) emission inventory is conducted by DARS. According to the result of this uncertainty assessment, the uncertainty for fugitive dust emission data is higher than other sources, the uncertainty of emission factor for surface coating is the highest value, and the uncertainty of activity data for motor cycle is the highest value. Also it is analysed that the improvement of uncertainty for activity data is as much important as the improvement for emission factor to upgrade the reliability of CAPSS emission inventory.

• 대한한의학회지
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• 제34권1호
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• pp.179-191
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• 2013

Kleine-Levin syndrome is a rare disorder characterized by recurrent episodes of hypersomnia, hyperphagia, hypersexuality and delusion and hallucination with spontaneous recovery. A 17-year-old female suddenly showed hypersomnia, severe fatigue, and compulsive hyperphagia. She was diagnosed with suspected Kleine-Levin Syndrome. The precipitating factor was an upper respiratory tract infection that the patient had undergone 2 weeks prior. Past psychiatric history included somnambulism and ADHD; her symptoms of ADHD included attention deficit, impulsiveness, hyperactivity, and irritability. This was her third episode. Her hypersomnia was longer in duration but lower in frequency compared with usual KLS. Her low LF/HF ratio in heart rate variability measurement showed high parasympathetic nerve activity. A MMPI report showed some psychological problem. The patient was diagnosed of gastric fever by pattern identification. Her hypersomnia episode improved 6 weeks after onset. This is the first reported case of suspected Kleine-Levin Syndrome in the field of Oriental Medicine in Korea.

• 한국산학기술학회논문지
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• 제20권6호
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• pp.158-168
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• 2019

본 연구는 국민건강영양조사 제7기 1차년도(2016년) 자료를 이용하여 청소년의 대사증후군과 식생활, 신체활동 및 정신건강과의 관계를 파악 및 분석하여 청소년의 대사증후군 예방과 정신건강 증진을 위한 기초적 자료를 제공하는데 그 목적이 있다. 연구대상자의 연령은 만13세-만18세 청소년으로, 그 중 정상군은 남자 201명(52.6%), 여자 187명(47.4%)으로 나타났으며, 대사증후군은 남자 9명(56.4%), 여자 6명(43.6%)으로 총 403명으로 나타났다. 이에 청소년의 대사증후군과 식생활, 신체활동 및 정신건강과의 관계를 분석하여 연구한 결과, 청소년의 대사증후군에 미치는 주요 영향요인은 다음과 같다. 첫째, 신체특성에서 BMI가 1 증가할 때 마다 대사증후군 유병확률이 1.746배 증가하는 것으로 나타났다. 둘째, 식생활습관에서는 아침식사 빈도에서 주1-2회 식사 시 거의 안할 때 보다 대사증후군 유병확률이 0.87배 감소하는 것으로 나타났다. 셋째, 신체활동에서는 평소 하루 앉아있는 시간이 1시간 증가할 때 마다 대사증후군 유병확률이 1.054배 증가하는 것으로 나타났다. 넷째, 청소년의 대사증후군은 정신건강과의 관련성에 미치는 영향이 없는 것으로 나타났다. 따라서 향후 청소년을 중심으로 한 대사증후군 관련 연구에 있어서 식생활, 신체활동 및 정신건강 등에 관한 다양한 변수들의 개발을 통해 보다 확장된 연구가 필요할 것으로 판단되며 또한 청소년의 대사증후군 예방을 위한 식생활요인과 신체활동요인 및 정신건강요인을 접목한 효과적인 건강교육 중재 프로그램개발이 필요하다.

• 대한간호학회지
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• 제38권2호
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• pp.217-228
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• 2008

Purpose: This study was conducted to develop and to determine the effects of an integrated symptom management program for prevention of recurrent cardiac events after percutaneous coronary intervention. Methods: Subjects consisted of 58 CAD patients (experimental group: 30, control group: 28). The experimental group participated in an integrated symptom management program for 6 months which was composed of tailored education, stress management, exercise, diet, deep breathing, music therapy, periodical telephone monitoring and a daily log. The control group received the usual care. Results: The experimental group significantly decreased symptom experiences and the level of LDL compared to the control group. The experimental group significantly increased self care activity and quality of life compared to the control group. Although no significant difference was found in cardiac recurrence, the experimental group had fewer recurrences. Conclusion: These results suggest that an integrated symptom management program for prevention of recurrent cardiac events after percutaneous coronary intervention can improve symptom aggravation, recurrent rate, self care activity and quality of life. Nursing interventions are needed to maintain and further enhance the quality of life of these patients and the interventions should be implemented in the overall transition period.

• The Korean Journal of Physiology
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• 제16권2호
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• pp.119-128
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• 1982

The frog truncus arterious were studied with conventional glass microelectrode technique in order to elucidate the underlying mechanism of spontaneous pacemaker activity. The analyses were focussed on the ionic nature of pacemaker current by changing the concentrations of extracellular $K^+$ and, $Na^+$, or by using blockers of K- and Ca-current and chronotropic transmitters. 1) The action potential of the spontaneously active truncus arteriosus has some characteristic feature of maximal distolic potential ranged from -65 to -75 mV, resting potential from -45 to -50 mV and overshoot voltage about +30 mV, respectively. Duration of the action potential taken from rapid upstroke to maximal diastolic potential was about 600 msec. Usual discharge rate was $25{\sim}30/min$ at room temperature $(18{\sim}20^{\circ}C)$. 2) The sensitivity of the resting membrane potential to change extracellular potassium concentrations $(0{\sim}12\;mM)$ was relatively low. Transient hyperpolarization was appeared in the 12 mM K Ringer after 10 min exposure to 0 mM K and it could be related to Na-pump reactivation by high potassium. 3) Reduction of extracellular sodium concetrations diminished the amplitude and frequency of the action potential. In Ringer solution containing 30% Na (substituted by equimolar Tris), spontaneous activity stopped but reappeared as very slow and small action potential. There was no spotaneous activity in zero Na Ringer solution. 4) Caesium(10 mM), K-current blocker decreased the frequency of the action potential and also pacemaker depolarization. Manganese (2 mM) known to be Ca-current antagonist, blocked spontaneous activity completely. 5) Adrenaline and acetylcholine had no chronotropic effect. But adrenaline increased the duration of plateau phase and the magnitude of the action potential in the follower cell. It is concluded that K-, Na-and Ca-current components are involved in the genesis of spontaneous activity of the frog truncus arteriosus like cardiac pacemaker tissues. But the insensitivity of truncus arteriosus to adrenaline and acetylcholine indicates that there are some different control mechanisms of spontaneous rhythm in two tissues.

• Tuberculosis and Respiratory Diseases
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• 제51권6호
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• pp.540-549
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• 2001

연구배경 : 특발성 폐섬유화증 중 통상성 간질성 폐렴(Usual interstitial pneumonia)은 대부분 진행성으로 악화되는 매우 나쁜 질환이다. 조직학적으로 볼 때 상피하에 흩어져서 존재하는 fibroblastic foci에서 콜라겐 합성을 통한 폐 섬유화를 일으키는 것으로 알려져 있다. 이러한 fibroblastic foci와 폐기능을 포함한 임상적 지표 및 예후와의 상관관계를 알아보고자 하였다. 방 법 : 개흉 또는 흉강경을 통한 폐 생겸검사로 진단된 20명(남:여 13:7)을 대상으로 하여 폐 조직에서 fibroblastic foci의 양을 정량화 하였다. 결 과 : 1) Fibroblastic foci 양과, 폐기능검사, 동맥혈가스검사($AaDO_2$) 사이에는 유의한 상관관계가 없었으며, 기관지폐포 세척검사의 세포분획중 호중구 비율과는 의미있는 비례관계(p<0.05)가 있었다. 2) Fibroblastic foci/vol을 0.2를 기준으로 두 그룹으로 나누어 각 그룹간의 생존률, 폐기능검사, 기관판지폐포 세척검사의 세포분획비율의 차이를 비교한 결과 기판지폐포세척액의 호중구비율이 fibroblastic foci가 많은 군에서 의미있게 높았으며(p<0.05), 생존율, 폐기능점사등은 두 군간에 유의한 차이는 없었다. 결 론 : 본 연구에서는 fibroblasic foci가 UIP 환자의 예후 및 질환의 활성도를 나타내주는 지표로는 확실하지 않은 것으로 여겨지며, 추가로 좀더 많은 자료로 분석이 필요할 것으로 여겨진다.

• Journal of Microbiology
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• 제37권3호
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• pp.148-153
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• 1999

Laccase produced by Coriolus hirsutus was purified to electrophoretic homogeneity by acetone precipitation, Sephacryl S-2000 HR chromatography, DEAE Sepharose CL-6B chromatography, and Mono Q HR 5/5 chromatography. The purification of laccase was 46.6-fold with an overall yield of 23.7%. Laccase from this fungus was a monomeric glycoprotein with 16% carbohydrate content, and has an isoelectric point of 4.2, and molecular mass of 78 kDa, respectively. The N-terminal amino acid sequence of the enzyme showed significant homology to hoste of laccases from Coriolus versicolor, Pycnoporus cinnabarius, and an unidentified basidiomycete, PM1. The highest rate of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) oxidation by laccase was reached at 45$^{\circ}C$, and te pH optima of the enzyme varied depending on the substrate in the range of 2.0 to 4.5. The enzyme was stable at 60$^{\circ}C$ for 5 h and lost 80% activity at 80$^{\circ}C$ in 30 min. The enzyme oxidized a variety of usual laccase substrates including lignin-related phenol, and had the highest affinity toward ABTS. Under standard assay conditions, the apparent Km value of the enzyme toward ABTS was 8.1 ${\mu}$M. The enzyme was completely inhibited by L-cysteine and sodium azide, but not by potassium cyanide, SDS, ad thiourea.