An experiment was conducted to investigate the effects of mineral extract from granite on the performance, ammonia production from the litter, components of blood, Newcastle Disease (ND) titer and intestinal microflora in broiler chickens. Nine hundred sixty one-day-old broiler chickens (Ross) were assigned to five treatments: C; control, Zeolite; control + zeolite 1$\%$, AM10: control + active mineral water $10\%$ adsorbed zeolite $1\%$, AM20; control + active mineral water $20\%$ adsorbed zeolite $1\%$ and AM30; control + active mineral water $30\%$ adsorbed zeolite $1\%$. Each treatment consisted of four replicates with 48 broiler chicks for feeding trial. In order to test the effect of ND vaccine on the components of blood, ND titer and intestinal microflora, a separate group of 48 broiler chicks were assigned to the same 5 treatment as the feeding trial plus one negative control (No ND vaccine). Weight gain, feed intake, feed conversion and mortality were not significantly affected by dietary treatments but AM30 tended to be higher than other treatments in weight gain and feed intake, especially during later period (4 to 5 weeks of age). Ammonia production from the litter of AM30 treatment was significantly (P<0.01) lower than the control. Components of blood and ND titer in serum of broiler chickens were not significantly affected by treatments but MCHC (mean corpuscular hemoglobin concentration) of blood was significantly lower (P<0.05) in Zeolite treatment compared to others. The colony forming unit (CFU) of Clostridium perfringens in the small intestinal content of all zeolite and AM treated groups was significantly (P<0.01) lower than the control while the CFU of Escherichia coli was not significantly affected. The CFU of Lactobacilli in AM30 treatment was significantly (P<0.05) higher than the control. In conclusion, dietary supplement of active mineral water adsorbed to zeolite at $30\%$ level (AM30) tended to improve growth performance of broiler chickens and significantly reduced ammonia production from the litter. It also significantly increased CFU of intestinal Lactobacilli.
This study was performed to examine the effects of dietary Acanthopanax senticosus (AS) on growth performance, immune organ weights, blood biochemical parameters and the expression of pro-inflammatory cytokines in broiler chicks. A total of 120 4-day-old birds were given a basal diet (CON) or a basal diet supplemented with 0.5% (AS1) or 1.0% (AS2) AS powder until the birds were 35 days of age. There was no difference in body weight, total gain, feed intake or immune organ weights among the treatment groups. However, the feed conversion ratio in the AS2 group was lower (p<0.05) than that in the CON group. Serum biochemical components, including AST (aspartate aminotransferase), ALT (alanine aminotransferase), albumin and total protein, were not affected by the dietary treatments, whereas glucose and triglyceride levels increased (p<0.05) in the AS2 group compared with the CON group. The AS1 group exhibited decreased mRNA expression (p<0.05) of IFN-${\gamma}$ in white blood cells and iNOS in the liver compared with the CON group. The other pro-inflammatory cytokines were unaffected by dietary AS supplementation, although there was a trend towards decreased expression of these genes, including those encoding Il-$1{\beta}$, IL-6 and TNF-${\alpha}$. In conclusion, dietary supplementation with 0.5% AS decreased the expression of several pro-inflammatory cytokines without affecting growth performance, suggesting that this supplement might be applicable as an immunoregulatory feed additive in broiler chicks.
The purpose of this study is to understand the vegetation characteristics of ridges (Gyeongsusan-Seonunsan-Gaeipalsan) in the Seonunsan Provincial Park and to establish reference information for the management of the park in the future. We designated 62 plots with the area of $100m^2$ were installed and analyzed them to investigate the vegetation characteristics. The results of community classification based on TWINSPAN showed seven categories of vegetation communities in the surveyed region: Quercus dentata-Deciduous broad-leaved Community, Quercus variabilis-Pinus thunbergii-Quercus serrata Community, Pinus densiflora Community, Deciduous broad-leaved Community-I, Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, Deciduous broad-leaved Community-II, and Carpinus tschonoskii-Carpinus laxiflora Community. In the vegetation of Seonunsan Provincial Park, coniferous trees such as Pinus thunbergii and Pinus densiflora have been gradually losing their population as part of ecological succession to deciduous broad-leaved trees such as Quercus spp., Carpinus tschonoskii, and Carpinus laxiflora. Moreover, Carpinus turczaninowii, Mallotus japonicus, and others were identified as vegetation reflecting the geographical characteristics of the region neighboring the west coast. The estimated age is 30-60 years, and the oldest tree Pinus densiflora is 63-years old. The index of diversity ($100m^2$) was 0.7942 for Carpinus tschonoskii-Carpinus laxiflora Community, 0.8406 for Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, 0.8543 for Quercus dentata-Deciduous broad-leaved Community, 0.9434 for Quercus variabilis-Pinus thunbergii-Quercus serrata Community, 0.9520 for Deciduous broad-leaved Community-I, 0.9633 for Pinus densiflora Community, and 1.0340 for Deciduous broad-leaved Community-II in the ascending order.
The current study was designed to define whether a blend of prunus mume extract(25%) containing lactic acid(75%) and grape seed extract(10ppm) could affect in vitro antimicrobial activity and growth performance, intestinal microflora, plasma biochemical profiles and digestive enzymes activities in broiler chickens. In paper disc agar diffusion test, we clearly observed antimicrobial activity against E. coli in response to prunus mume extract or a blend of prunus mume extract. For in vivo test, a total of ninety six 3-d-old male broiler chicks were assigned to basal diet(CON), basal diet supplemented with antibiotics (ANTI) and 0.5% a blend of prunus mume extract(PRNUS) until 35 days of age. Throughout the entire experimental period(3-35 days), there were no differences in BW and FCR between the birds fed the basal diet with antibiotics and the diet supplemented with a blend of prunus mume. However, ANTI group showed a significant increase in BW and total gain compared to CON group. The weights of digestive organs such as the pancreas and mucosal tissues were not affected by dietary treatments. There was no difference in plasma levels of glucose, cholesterol, AST and ALT activity. However, triglyceride in plasma increased(P<0.05) in the birds fed the diet supplemented with 0.5% a blend of prunus mume extract compared to those fed antibiotics supplemented diet. The activities of pancreatic trypsin and amylase, and intestinal hydrolase including disaccharidase were not affected by dietary treatment. The colony forming units(CFU) of lactobacillus in the lower ileal-cecum of the birds fed the diet supplemented with a blend of prunus mume extract was significantly(P<0.05) higher than that of birds fed antibiotic supplemented diet without affecting the CFU of E. coli. In conclusion, the birds fed the diet supplemented a blend of prunus mume as an alternative to antibiotics showed a similar growth performance and an significant increase in lactobacillus population compared with the birds fed basal and antibiotics supplemented diets.
Purpose: To evaluate the risk of gastrointestinal bleeding associated with use of low-dose aspirin in children. Methods: Among about 250 children who received low-dose aspirin (5 mg/kg/day) under the diagnosis of Kawasaki disease, from March 1995 to May 2001, at Eul-Ji general hospital, 100 children were enrolled in this study. We reviewed the medical records and interviewed the children's parents over the phone to confirm the existence of gross gastrointestinal bleeding. Results: The age of the children at the beginning of medication ranged 4~118 months. About 75% of them was younger than 3 years old. The duration of medication ranged 0.5~17 months. About 70% of the children took the medicine for 2~3 months. Only 1 child (1%) had hematochezia during medication without any accompanying gastrointestinal symptom, and cimetidine for 1 week had cleared up the bleeding. The total duration of medication of 100 children was 341.5 months, and only 1 child had gastrointestinal bleeding. This translates into a rate of clinically significant gastrointestinal bleeding of 3.5 episodes/100 children/year. Conclusion: The long-term use of low-dose aspirin is safe, but, is associated with the risk of gastrointestinal bleeding in children. Careful follow-up and efforts to reduce the risk of gastrointestinal bleeding are necessary during long-term low-dose aspirin therapy in children.
Purpose : The purpose of this study was to identify the current status of symptom and pain control in cancer patients treated with chemotherapy. Methods : The study population consisted of 66 cancer patients treated with chemotherapy and the data was collected by chart audit and using questionnaire from 19 clinicians in an university hospital during the period of August 7 to 24, 2001. The degree of symptom, analgesics type, dose, administration route, and pain control method of cancer patients treated with chemotherapy was investigated. The collected data were analyzed by frequency, percent, mean, and SD using SPSS $PC^+$ program. Results : The number of cancer patients treated with chemotherapy was 66, male 35 (53.0%), female was 31 (47.0%). The largest of age group was $61{\sim}69$(34.8%) years old. Most frequent cancer site was stomach 19 case (28.8%), cole-rectal 17 case (25.8%), urinary 11 case (16.7%) in the respective order. The most common stage of cancer was stage 3, 14 case (29.2%). The most frequent duration of diagnosis was under 3 month, 25 (37.9%). The frequent symptom of cancer patients treated with chemotherapy was marasmus ($2.4193{\pm}1.2220$), pain ($1.9333{\pm}1.2194$), sleep disorders ($1.7142{\pm}1.0384$), personality change ($1.5806{\pm}0.8971$) in the respective order. 3) The analgesics used for pai control were narcotic analgesics 66.2% and nonnarcotic analgesics 33.8%. Pain control method were regular basis+as needed 47.4%, as needed 31.6%, regular basis 21.0% in order. Administration route were oral 50.7%, injection 41.8%, patch 7.5% in order. Conclusion : The most frequent symptom of cancer patients treated with chemotherapy were marasmus and pain. The frequent analgesics was narcotic analgesics but 21% of the clinician regularly prescribed analgesics for pain control. Thus this prescription was insufficient for pain control. Administration route that were used more oral or injection than patch. Based on this evidence, it seemed that more active practice and education about evaluation and pain control of cancer patients treated with chemotherapy are needed.
The Journal of the Korean bone and joint tumor society
/
v.10
no.1
/
pp.13-21
/
2004
Purpose: We analyzed the result of autologous bone marrow stromal cell transplantation with or without cancellous chip bone allograft for benign long bone lesions. Materials and methods: Since July 1996, eight benign bone lesions treated by curettage, cancellous chip bone allograft and bone marrow or marrow stromal cell transplantation were observed for resolution of clinical symptoms, new bone formation and consolidation. There were 6 males and 2 females. Average age was 24 (range 8 to 47) years old. Histologic diagnoses were 5 fibrous dysplasia, 2 simple bone cysts and one chondroblastoma and fibrous cortical defect each. Mean follow-up period was 16.3 (range 3 to 84) months. Results: In all four symptomatic patients, the pain was subsided in two weeks after surgery. New bone formation in the lesion was observed at 4 weeks, which incorporated into surrounding normal bone around 8 weeks. There were one pathologic fracture through the lesion at 3 weeks and one recurrence of simple bone cyst at 5 months postoperatively. Conclusion: Bone marrow or marrow stromal cell transplantation for bone defects from curettage of benign bone lesions, with or without cancellous chip bone allograft revealed rapid healing. Though it was the result of short-term follow up, it supports that bone marrow stromal cell transplantation will be very useful for the treatment of benign long bone cysts or other lesions. The complete curettage of inner cystic wall is important to prevent later recurrence, and the rigid internal fixation is also needed in selected high risk lesions of fracture.
The aim of this study was to determine the effects of stocking density and strain on the performance and physiological adaptive responses including the plasma corticosterone content and the level of mRNA expression of pro-inflammatory cytokines and antioxidant enzymes in broiler chicks. A total of 300 birds of two strains (150 Ross strain vs. 150 Cobb strain) aged 3-d old were allotted into two stocking densities (standard stocking density,$0.046m^2/bird$ vs. high stocking density, $0.023m^2/bird$) in battery cages by $2{\times}2$ factorial designs with ten replicates until 35 d of age. There was no significant strain effect on body weight, feed intakes and feed to gain ratio and the relative organ weights. However body weight, feed intakes and relative organ weight were found to be significantly (P<0.05) affected by the effect of stocking density. Plasma corticosterone level was not affected by both stocking density and strain effects. Hepatic mRNA expression of pro-inflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, IL-18 and interferon-gamma (IFN-${\gamma}$) was not significantly changed by the effects of strain and stocking density. However, the mRNA expression of glutathione peroxidase (GPX) was affected by strain, showing that Ross strain decreased (P<0.05) the GPX expression. With respect to the effect of stocking density, there was a significant (P<0.05) increase in superoxide dismutase (SOD) and catalase (CAT) and GPX mRNA expression in the liver from high stocking density group. Splenic pro-inflammatory cytokine expression was not also affected by stocking density and strain, except that IL-18 mRNA significantly (P<0.05) decreased in Cobb strain under high stocking density. The mRNA expression of SOD and CAT was significantly (P<0.05) affected by the effects of stocking density and strain. In conclusion, growth performance was not affected by strain but stocking density. Although mRNA expression of major pro-inflammatory cytokines was not changed by stocking density and strain, antioxidant enzyme was significantly affected by stocking density, strain or even organ in birds under summer conditions. More detailed studies still needed to be explored to elucidate the effects of environmental conditions and genetic background on physiological responses in birds.
Purpose: This study evaluated the static stereoacuity by Distance Randot Stereotest (STEREO OPTICAL. Co., Inc. USA) and the dynamic stereoacuity by three-rods test (iNT, Korea). Criterion and correlation of stereoacuity between both tests and usefulness of two stereotest methods were also evaluated. Methods: For normal adults of 109 (male 61, female 48), mean age of 20.88 (19-32 years) years old, static stereoacuity by using Distance Randot Stereotest at 3 m distance, dynamic stereoacuity by using three-rods test at 2.5 m distance were measured. Results: The mean of distance static stereoacuity was $155.77{\pm}133.11sec$ of arc and the mean of error distance dynamic stereoacuity $11.13{\pm}9.69mm$. With equivalent-conversion stereoacuity of $23.44{\pm}20.96sec$ of arc, there was statistically significant differences (p=0.00) between two dynamic stereoacuity, but correlation was relatively low (${\rho}=0.226$). In the case of dynamic stereoacuity, separated to normal range by criterion of the error distance 20 mm, it showed the error distance of less than 20 mm in 97 subjects(89%) whose average of error distance and conversion mean dynamic stereoacuity were $8.43{\pm}5.10mm$ and $17.68{\pm}10.67sec$ of arc. repectively. The error distance of was equivalent-conversion dynamic stereoacuity 40.99 sec of arc (PD 62 mm basis) was 20 mm. Conclusions: The results of lower correlation between static and dynamic stereoacuity suggest that seterotest should be applied separately to different functions. The results of this study also suggest that Distance Randot Stereotest can be applied to static stereoacuity excluding monocular cues. Three-rods test can be applied to dynamic stereoacuity containing the response of the eye-hand coordination in the daily life of natural vision condition, including the monocular cues. These different approaches canprovide a criterion of the two stereoacuity and parallel use of the two tests would be useful. For dynamic stereoacuity by three-rods test, error distance 20 mm in a normal range of adults can be used as a criteria to get statistical meaning of the results.
Purpose: On this study, we compared the relationship of dynamic stereoacuity according to the dominant eye, degree of dominant eye, and dominant agreement eye and hand. Methods: For 130 adults (male 70, female 60), mean age of $21.06{\pm}2.21years$ old, dominant eye, degree of dominant eye were measured by objective examination by using the diameter $3.8cm{\times}3.8cm$ thin ring, the dynamic stereoacuity were measured by three-rods test (iNT, Korea). Results: Dynamic stereoacuity according to the dominant eye was center dominant eye without dominance was $14.97{\pm}13.80sec$ of arc, right eye $22.10{\pm}20.01sec$ of arc, left eye $22.31{\pm}20.39sec$ of arc. Dynamic stereoacuity was better when there was no dominance, but the correlation of the dominant eye with dynamic stereoacuity was very low. When Dynamic stereoacuity was separated by in the Center, Mild, Strong, dynamic stereoacuity was $14.97{\pm}13.80sec$ of arc, $20.76{\pm}15.73sec$ of arc and $24.45{\pm}25.60sec$ of arc respectively. The dynamic stereoacuity results were worse when dominance was stonger. However dynamic stereoacuity was better than Center when the degree of dominant eye was rather strong in the dominant left eye. Dynamic stereoacuity according to the dominant eye and hand showed that right eye and hand was $22.63{\pm}20.54sec$ of arc, left eye and hand was $17.36{\pm}10.13sec$ of arc, right eye and left hand was $14.79{\pm}7.05sec$ of arc, left eye and right hand was $22.97{\pm}21.42sec$ of arc so dynamic stereoacuity was comparatively good when the dominant hand was left. Conculsions: Correlation between the dynamic stereoacuity according to the dominant eye, degree of dominant eye was low, however when degree of dominant eye was Center 14.97 sec of arc, Strong 24.45 sec of arc, the dynamic stereoacuity tended to worse when degree of dominant eye was strong. As a result, dominant eye, degree of dominant eye would have to be considered in a more comfortable binocular balance between prescribed for the wearer in binocular vision correction in binocular function such stereoacuity, sports vision training, presbyopia correction and mono vision.
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