• Journal of Life Science
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• v.33 no.10
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• pp.776-782
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• 2023

Silymarin, which is derived from dried Silybum marianum (milk thistle) seeds and fruits, possesses various beneficial properties, such as hepatoprotective, antioxidative, anti-inflammatory, and anticancer activity. This research aimed to explore the antioxidative activity of silymarin against oxidative stress and understand its molecular mechanism in RAW 264.7 cells. The study employed cell viability and reactive oxygen species (ROS) formation assays and western blot analysis. The results demonstrated that silymarin effectively reduced intracellular ROS levels induced by lipopolysaccharide (LPS) in a dose-dependent manner without causing any cytotoxic effects. Moreover, silymarin treatment significantly upregulated the expression of heme oxygenase (HO)-1, a phase II enzyme known for its potent antioxidative activity. Additionally, silymarin treatment significantly induced the expression of nuclear factor-erythroid 2 p45-related factor (Nrf) 2, a transcription factor responsible for regulating antioxidative enzymes, which was consistent with the upregulated HO-1 expression. To investigate the involvement of key signaling pathways in maintaining cellular redox homeostasis against oxidative stress, the phosphorylation status of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) was estimated by western blot analysis. The results showed that silymarin potently induced HO-1 expression, which was mediated by the phosphorylation of p38 MAPK. To further validate the antioxidative potential of silymarin-induced HO-1 expression, tert-butyl hydroperoxide (t-BHP)-induced oxidative damage was employed and attenuated by silymarin treatment, as identified by a selective inhibitor for each signaling molecule. In conclusion, silymarin robustly enhanced antioxidative activity by inducing HO-1 via the Nrf2/p38 MAPK signaling pathway in RAW 264.7 cells.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.236-242
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• 2012

This study was carried out to investigate the effect the water content in coir substrates as according to growth stage on the growth and yield of mini-paprika of three varieties 'E 499524' (red color), 'E 499526' (yellow colar) and 'E 499531' (orange colar) in summer hydroponics. Treatments of I, II and III were composed of 55-65-60, 50-60-55 and 45-55-50% in water contents of growth stages, respectively. Time domain reflectometry (TDR) sensors were used in a drip irrigation system. The early growth of mini-paprika was increased by high medium water content of treatment I in all of three varieties. Mean fruit weight was not affected by medium water content, but the fruit number per plant and yield were increased at high medium water content of treatment I. The Yield of treatment I was higher than that of treatment II and III in all of three varieties. The sugar content was increased by low medium water content of treatment in all of three varieties. The incidence of brown-stem fruit, blossom-end rot and sunburn was decreased with increasing water content of medium. Occurred in the low water content of medium. Mineral contents of fruits such as nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg) etc. were not affected in all of three varieties.

• Korean Journal of Food Science and Technology
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• v.44 no.5
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• pp.540-544
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• 2012

The aim of this study was to evaluate the antioxidant activity, total phenolics, flavonoids, and vitamin C contents in peels of 11 commercial fruits commonly consumed in the Republic of Korea. The antioxidant activities were evaluated by ABTS, DPPH radical scavenging methods and reducing power. The peels of plum (Prunus spp.) and golden kiwifruit (Actinidia chinensis) exhibited the highest values of antioxidant activity and total phenolics, while the highest level of ascorbic acid was recorded in the peel of citrus fruit (p<0.05). There were positive correlations between the antioxidant activity assessed by both reducing power and ABTS methods and total polyphenol content ($R^2$=0.6356), suggesting that phenolic compounds are the major contributors to these activities. The results indicate that plum and golden kiwifruit peels can be used as natural antioxidant ingredients and in production of functional foods in the Korean diet.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.145-151
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• 1997

An unusual young fruit rot of apple caused by two species of Phytophthora was epidemic from late May to early July of 1996 in Andong, Uisung and Chungwon areas of Korea. The disease spread to over 30 apple orchards in the areas and percent of the infected tree and fruit was ca. 10~90% and 1`15%, respectively. Water soaking lesions or spots on leaves and shoot blight were also developed by the pathogen. Among 39 isolates collected, 25 were identified as P. cactorum and the others were as zp. cambivora on the basis of their distinctive morphological characters. While the former fungus was homothallic, all isolates of the latter were A1 mating types. Koch's postulate was fulfilled. Both fungi showed strong pathogenicity not only to young fruits, leaves and shoots of apple but also to those of pear and peach. Several vegetables tested did not show symptoms even by wound inoculation. An Occurrence of young fruit rot of apple caused by Phytophthora has not been reported in Korea, especially, P. cambivora has not been recorded previously as the causal agent of the disease in the world.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.31-36
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• 2005

Adoxophyes spp. are the major rests of a pear. The larvae attack both leaves and fruits. (Z)-9-tetradecenyl acetate (Z9-14:Ac), (Z)-11-tetradecenyl acetate (Z11-14:Ac), (E)-11-tetradecenyl acetate (E11-14:Ac) and 10-methyldodecyl acetate (10me-12:Ac) have been reported as the sex pheromone components of the genus Adoxophyes. Our objective was to determine the difference in sex pheromone composition of three different A. spp. populations each from Cheonan, Sangju, and Naju area orchards in Korea. Gas chromatography (GC) analyses of pheromone gland extracts of virgin females confirmed the presence of two compounds (Z9-14:Ac and Z11-14:Ac) in Cheonan and Sangju populations, and four compounds (Z9-14:Ac, Z11-14:Ac, E11-14:Ac and tome-12:Ac) in Naju population. The Z9-14:Ac and Z11-14:Ac were detected in the ratio of 80:20 in the Cheonan population and 3:97 in the Sangju population. Females of Naju population produced sex pheromone blend consisting of Z9-14:Ac, Z11-14:Ac, E11-14:Ac and 10me-12:Ac at a ratio of 31:62:6:1. Field trapping tests in pear orchards with Z9-14:Ac and Z11-14:Ac indicated that maximum captures of the male were obtained with traps baited by 80:20 in Cheonan, 10:90 in Sangju, and 30:70 in Naju. These results suggest that there are remarkable geographical variations in the sex pheromone composition of A. spp. in pear orchards in Korea, and taxonomic classification of these species must be carefully assessed.

• Journal of Life Science
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• v.12 no.6
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• pp.752-758
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• 2002

This study was investigated on properties and thermostability of gelatin-degrading proteinases in the fruit of Actinidia chinensis (kiwifruit) for the industrial application. Three gelatin-degrading proteinases (PI, PII and PIII) were detected from the pulp of fruits. The molecular weights of these proteinases, PI, PII and PIII, were approximately 220 kD, 51 kD, and 26 kD respectively, on the basis of gelatin-containing SDS-PACE. The optimum pH of these proteinases ranged from 2.0 to 5.0 with a maximal activity at pH 4.0. These proteinases had a high sensitivity to E-64 and iodoacetate which are cysteine protease inhibitors, and required DTT, cysteine, and $\beta$-mercaptoethanol for their activities which are stimulators for cysteine proteases. These results indicate that these proteinases are cysteine proteinases and the proteinase PIII is actinidin (EC 3.4.22.14), based on the molecular weight and/or susceptibility against proteinase inhibitors. These proteinases were strongly activated by $Ca^{2+}$, $Mg^{2+}$ and $Mn^{2+}$, whereas strongly inhibited by Zn$^{2＋}$ and Hg$^{2＋}$. However, these proteinases have slightly different susceptibility against other cations ($Ca^{2+}$, $Cu^{2+}$, $Al^{3+}$, $Ca^{3+}$. The temperature stability of proteinase PIII was more stable than proteinases PI and PII. Moreover, proteinase PIII remained stable below $50^{\circ}C$ for 48hr, showing the residual activity above 75% of the enzyme activity.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.3
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• pp.171-176
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• 2004

To investigate the availability of nitrogen decomposed and released from liquid pig manure (LPM), this experiment was performed with red pepper (Capsicum annuum L., cv. Hanbando), and Chinese cabbage (Brassioa campestris L., cv. Konaenggiyeureumbaechoo) in 2001. Based on the total nitrogen of chemical fertilizer, both red pepper and chinese cabbage were treated with three and four applications of LPM, respectively. Yield of red fruits in the red pepper was increased by an enhancement of LPM application. However, that of chinese cabbage was enhanced with a reducing supply of LPM. Biosynthesis of polyamine in both crops such as red pepper and chinese cabbage was large in the early growth stage and was not increased by LPM application. The high biosynthesis of bound polyamine, monoamine and diamine, in the early growth stage was changed in an increase of conjugated polyamine and polyamine with a process of crop growth. Inorganic components in the leaf of red pepper by LPM application were equal or slightly lower than in chemical fertilizer, however, from the middle growth stage, contents of phosphate and potassium were increased. Those of chinese cabbage were slightly decreased from the early growth stage to the late. Considering this experiment, the thoughtless supply of LPM has not resulted in certain crop damages, and an application of LPM to increase a yield was different from crop species at some extent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1715-1719
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• 2004

In order to help the preservation of the unshiu orange and pear, antimicrobial paper incorporating grapefruit seed extract and zeolite was applied to pack fruits. Unshiu orange was packed in a box (24${\times}$24${\times}$22 cm) attached with antimicrobial paper and then stored respectively at l$0^{\circ}C$. Pears were wrapped individually before storage at l$0^{\circ}C$. During the storage, weight loss, pH, total acidity, soluble solid content, microbial load and decay were measured as quality indices. Steady pH increase in unshiu orange was observed to slightly decrease total acidity during the storage with little difference between the packaging treatments. The microbial loads of total aerobic bacteria, and yeast/mold counts were suppressed during storage by the antimicrobial paper packaging, which also contributed to reducing the decayed unshiu orange. Limited reduction of total aerobic bacteria and yeast/mold counts was observed only for initial storage period for the pears wrapped with 9 and 12% antimicrobial agent-added papers. Antimicrobial paper was useful for the reduction of microbial load in unshiu orange and pear without other quality deterioration.

• Nutrition Research and Practice
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• v.11 no.3
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• pp.247-256
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• 2017

BACKGROUND/OBJECTIVES: Easy access to intervention and support for certain behaviors is important for obesity prevention and management. The available technology such as smartphone applications can be used for intervention regarding healthy food choices for obesity prevention and management in elementary-school students. The transtheoretical model (TTM) is comprised of stages and processes of change and can be adopted to tailored education for behavioral change. This study aims to develop TTM-based nutrition contents for mobile applications intended to change eating behaviors related to weight gain in young children. SUBJECTS/METHODS: A synthesized algorithm for tailored nutrition messages was developed according to the intake status of six food groups (vegetables, fruits, sugar-sweetened beverages, fast food and instant food, snacks, and late-night snacks), decision to make dietary behavioral changes, and self-confidence in dietary behavioral changes. The messages in this study were developed from December 2014 to April 2015. After the validity evaluation of the contents through expert consultation, tailored nutrition information messages and educational contents were developed based on the TTM. RESULTS: Based on the TTM, stages of subjects are determined by their current intake status, decision to make dietary behavioral changes, and self-confidence in dietary behavioral changes. Three versions of tailored nutrition messages at each TTM stage were developed so as to not send the same messages for three weeks at most, and visual materials such as figures and tables were developed to provide additional nutritional information. Finally, 3,276 tailored nutrition messages and 60 nutrition contents for applications were developed. CONCLUSIONS: Smartphone applications may be an innovative medium to deliver interventions for eating behavior changes directly to individuals with favorable cost-effectiveness. In addition, using the TTM for tailored nutrition education for healthy eating is an effective approach.

• The Korean Journal of Pesticide Science
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• v.9 no.2
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• pp.166-172
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• 2005

Temporary control schedules were tested at sweet persimmon orchards to development new control programs to meet the quarantine requirements of USA in 2002. The 'USA export-type control orchards' were spayed with pesticides (azoxystrobin, carbaryl, cyhexatin, fenarimol, mancozeb, methoxyfenozide, spinosad and trifluxistrobin) which were possibly adaptable to the poem trees in USA. Pesticide residues in the sweet persimmon fruits harvested from USA export-type control orchards were analyzed. Azoxystrobin, mancozeb, trifloxystrobin, spinosad, carbaryl, and cyhexatin were not detected by the experimental methods. The residues of fenarimol and methoxyfenozide in sweet persimmon of USA export-type control orchards were 0.016-0.020 ppm and 0.022-0.029 ppm, respectively. These levels are quite below the maximum residue limit level of USA (below 0.1 ppm in fenarimol and 7 ppm in methoxyfenozide). These results suggest that new control programs could be developed by modifying the USA export-type control schedule tested in this study to meet the quarantine requirements of USA, if we could suppress the damage of plant bugs.