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Development of Tetraploid Watermelon Using Chromosome Doubling Reagent Treatments (염색체 배수화제를 이용한 4배체 수박품종 개발)

  • Oh, Sang A;Min, Kwang Hyun;Choi, Yong Soo;Park, Sang Bin;Kim, Young Cheol;Cho, Song Mi
    • Korean Journal of Plant Resources
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    • v.28 no.5
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    • pp.656-664
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    • 2015
  • To produce high quality watermelon, three tetraploid watermelon breeding lines (‘SA03-1’, ‘SA06-1’ and ‘SB01-1’) were developed by treatment with different chromosome doubling reagents. To identify the optimal tetraploid inductive conditions, the three watermelon breeding lines were selected by counting the number of doubled chloroplasts in guard cells. Tetraploid induction rates differed depending on the genotypes and treatment with doubling reagents. However, the highest induction rate occurred with 1.0% colchicine (82.2%). These putative tetraploid lines were re-confirmed for ploidy using flow cytometric analysis and chromosome counting. The internode length of the tetraploid breeding lines was different when the leaf size was larger in all three tetraploid lines compared to their diploids. The fruit weight of the tetraploid fruits for ‘SA03-1’ and ‘SB01-1’ was lower than for their diploid, and the rind thickness and total sugar content (°Brix) of tetraploid SB01-1 were significantly different from those of its diploid. Tetraploid lines were sterile, yielded a lower number of seeds per fruit for ‘SA03-1’ (21), ‘SA06-1’ (62), and ‘SB01-1’ (34.7), and the seeds were larger and thicker than those of their diploids. These tetraploid breeding results will be useful for breeding new seedless watermelon cultivars.

Analysis on the Components of the Elaeagnus multiflora Thunb. Leaves (뜰보리수 잎의 유용성분 분석)

  • Yoon, Kyung-Young;Hong, Ju-Yeon;Shin, Seung-Ryeul
    • Food Science and Preservation
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    • v.14 no.6
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    • pp.639-644
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    • 2007
  • This study analyzed components of the leaves of Elaeagnus multiflora as part of studies on the nutritional and functional materials of fruits and leaves of this plant. The moisture content of the leaves was 71.6% and the carbohydrate, crude protein, lipid and ash contents were 24.1, 1.4, 0.4 and 2.5%, respectively. Concentrations of reducing sugars, soluble proteins and polyphenols were 460.0, 503.3 and 805.6 mg/100 g, respectively. Fructose was the dominant free sugar, and arabinose, maltose, glucose, and a small amount of trehalose were also detected. Malic acid was the main organic acid in E. multiflora leaves, and acetic acid, citric acid, lactic acid, and succinic acid were also present. E. multiflora leaves were high in K, Ca and Mg. Of hydrolyzed amino acids, alanine was present at the highest concentration (112.0 mg/100 g), with threonine, leucine, valine and phenylalanine being the next most common. Glutamic acid and ornithine were the dominant free amino acid and amino acid derivative, respectively.

Fermentation of Black Garlic Wine and its Characteristics (흑마늘 와인의 발효 및 그 특성)

  • Ha, Sung Min;Choi, Hye Jung;Shin, Gyeong Yeon;Ryu, Beung Ho;Joo, Woo Hong
    • Journal of Life Science
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    • v.26 no.7
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    • pp.796-804
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    • 2016
  • In the present study, we screened suitable yeasts for wine fermentation and evaluated the fermentative characteristics of Saccharomyces sp. BCNU 6006 and its anti-oxidant activities. Firstly, various yeasts were isolated from Makgeolli, fruits, and fermented foods. Then, the preliminary selections of suitable yeasts were made using an enzymatic activity assay of glucosidase, glycosidase, protease and tolerance to ethanol and SO2. In addition, the production of biogenic amines and hydrogen sulfide was also monitored. The 9 yeast strains initially selected were determined to belong to the genera Saccharomyces and Kazachtania phylogenetically. We investigated the optimal conditions for wine fermented with black garlic juice (BGJ). The optimal conditions of alcohol fermentation using BGJ were 26 brix, 28℃, and 10 days. Finally, the fermentation products of black garlic wine (BGW) fermented with Saccharomyces sp. BCNU 6006 exhibited 15.03% ethanol, 12 brix of sugar, and pH 4.01. The contents of total polyphenol, total flavonoid, tannin, and 5-HMF compound of BGW were 3.85 mg/ml, 0.51mg/ml, 5.90 mg/ml, and 0.07 mg/ml respectively, lower than that of BGJ. DPPH radical scavenging activity, ABTS radical scavenging activity, and reducing power of BGW were 90.77%, 95.20% and 1.261 respectively, lower than that of BGJ. Superoxide anion (O2-) radical scavenging activity was 94.42%, higher than that of BGJ. Based on the above results, the industrial potential of Saccharomyces sp. BCNU 6006 as a wine-making yeast was confirmed in the present study.

Effects of Mulberry Fruits on Collagen Content of Connective Tissues in Ovariectomized Rats (오디가 난소를 절제한 흰쥐 결함조직 중의 collagen 함량 변화에 미치는 영향)

  • Choi Kyung-Ha;Park Mi-Hwa;Kim Mihyang
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.1022-1027
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    • 2005
  • The aim of this study was to evaluate effects of mulberry cultivars extracts on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with $80\%$ ethyl alcohol extracts from various kinds of Tajikistan mulberry, Korea mulberry and China mulberry at 200 mg/kg bw/day, respectively. The mulberry extracts were orally administrated at 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats were at all time points, but supplementation with the mulberry extracts tended to gain weight less than OVX-control did. The ovariectomy caused a decreasing in the levels of collagen content in bone and cartilage tissues. However, supplementation with mulberry extracts prevented the decrease in the collagen level in bone and cartilage tissues. Therefore, it may be used to possibly improve the quality of life in menopausal women.

Studies on the Radiosensitivity and Mutation Induction of Tree Species in Korea (주요수종(主要樹種)의 방사선감수성(放射線感受性) 및 변이(變異)에 관(關)한 연구(硏究))

  • Kim, Chi Moon
    • Journal of Korean Society of Forest Science
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    • v.25 no.1
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    • pp.73-79
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    • 1975
  • The Koran native economic and ornamental trees consisting 12 species of 4 gymnosperm families and 3 strains, 6 varieties and 18 species of 12 angiosperm families were irradiated by X-and ${\gamma}$-ray at three different dose rates; 1) acute, 2) semi-acute, and 3) chronic irradiation in order to know their radiosensitivities and the radition effects on mutation induction. Different materials such as seeds, cuttings, scions and plants of the above trees were used in this study, depended upon tree. Most of the materials irradiated showed a high radiosensitivity. The LD-50 of conifer trees ranged from 1.2kR to 13.2kR, averaging 5.4kR which was remarkably higher than field crops. On the other hand the LD-50 dose of hard wood trees ranged from 7.0kR to 18.5kR, averaging 12.7kR. All the tested trees were classified into several categories based upon the relative radiosensitivity of each species which was measured at the dose of LD-50. Variegation was most common among the induced mutants. The other mutations were albino, chlorophyll deficiency, deformed leaf and fruits. It was noticeable that giant-leaf bud-sport mutants were induced in Chinese chestnut (Castanea bungeana) and yellow leaf bud-sport ones appeared in oriental arborvitae (Thuja orientalis).

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Analysis of the quality characteristics of Kochujang prepared using pastes from different peach varieties (복숭아 품종별 페이스트를 이용한 고추장의 품질 특성)

  • Jung, Kyung-Mi;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.19-26
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    • 2018
  • In this study, we aimed to increase the utilization of non-commercializable peach fruits collected during harvesting and distribution and to promote the transition of a high value-added industry. After producing pastes from the flesh of different peach varieties, we used these pastes as ingredients in the production of Kochujang. We then performed a comparative analysis on the quality characteristics of the peach-added Kochujang and commercially available Kochujang products. Peach-added Kochujang exhibited a lower pH and reduced sugar, salinity, reducing sugar, ash, and calorie content than those of commercially available Kochujang products. However, the Kochujang had slightly higher pH and moisture content than commercially available products due to high organic acid and moisture contents of peach flesh. In terms of content of inorganic elements, commercial Kochujang products exhibited higher sodium and magnesium content, whereas peach-added Kochujang had higher potassium content. In particular, the lower sodium and higher potassium content of peach-added Kochujang indicates that this product could be used as a functional food to prevent various diseases caused by excessive sodium intake among Koreans. After comparing the products in terms of phenol and flavonoid content, we concluded that ethanol extracts of peach-added Kochujang showed higher phenol content than those of the commercially available Kochujang products. In contrast, the distilled water extracts of peach Kochujang showed higher DPPH free radical scavenging activity than that of commercially available Kochujang products.

The Roles of Dietary Polyphenols in Brain Neuromodulation (뇌 신경조절에서의 식이 폴리페놀 화합물의 역할)

  • Lee, Hyeyoung;Lee, Heeseob
    • Journal of Life Science
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    • v.28 no.11
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    • pp.1386-1395
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    • 2018
  • Over recent years, it has become evident that the central nervous system bidirectionally interacts with the gastrointestinal tract along the gut-brain axis. A series of preclinical studies indicate that the gut microbiota can modulate central nervous system function through a multitude of physiological functions. Polyphenols are ubiquitous plant chemicals included in foods such as fruits, vegetables, tea, coffee and wine, and their consumption is directly responsible for beneficial health effects due to antioxidant, anti-inflammatory, antimicrobial, immunomodulatory, anticancer, vasodilating, and prebiotic-like effects. There is increasing evidence that dietary polyphenol can contribute to beneficial effects in neuronal protection acting against oxidative stress and inflammatory injury as well as in cognitive functions. In this paper, we overview the neuroprotective role of dietary polyphenols especially focusing on the neuroinflammation and neurovascular function by interaction with the gut microbiome. Polyphenol metabolites could directly act as neurotransmitters crossing the blood-brain barrier and modulating the cerebrovascular system or indirectly modulating gut microbiota. In addition, evidence suggests that dietary polyphenols are effective in preventing and managing neurological disorders, such as age-related cognitive decline and neurodegeneration, through a multitude of physiological functions. Dietary polyphenols are increasingly envisaged as a potential nutraceuticals in the prevention and treatment of neurological disorders, because they possess the ability to reduce neuroinflammation, to improve memory and cognitive function and to modulate the gut microbiota.

Antioxidant and Antibacterial Activities of Extracts from Different Parts of Sophora japonica L. (회화나무 부위별 추출물의 항산화 및 항균활성)

  • Park, Min Jeong;Kim, Hye Soo;Kim, Han Bi;Lee, Sang Gyun;Cho, Soo Jeong
    • Journal of Life Science
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    • v.32 no.10
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    • pp.792-802
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    • 2022
  • In this study, the antioxidant and antibacterial activities of Sophora japonica extracts were investigated to determine the potential of S. japonica as a functional food and medicinal materials. S. japonica was divided into flowers, fruits, and branches, and ethanol extraction was used. The total polyphenol and flavonoid contents were significantly higher in the flower and fruit extracts than in the branch extracts, but the ABTS and DPPH radical scavenging activity and ORAC value were higher in the branch extracts. Among the ethanol extracts of S. japonica, branch extracts showed strong antibacterial activity against Porphyromonas gingivalis, and the MIC was 0.2 mg/ml. Branch extracts showed bacteriostatic activity against P. gingivalis at a concentration of 0.4 mg/ml or less and bactericidal activity at a concentration of 0.6 mg/ml or more. Biofilm biomass production and cell growth of P. gingivalis in the culture medium treated with the branch extract at a concentration of 0.2-2.0 mg/ml were significantly decreased in a concentration-dependent manner. In addition, the mRNA expression of fimA and mfa1 associated with fimbriae formation in these cultures was suppressed in a concentration-dependent manner. Based on these results, S. japonica branch extracts can be used as functional food and medicinal materials, as demonstrated by their antioxidant and antibacterial activities against P. gingivalis and the inhibition of biofilm formation resulting from P. gingivalis.

Drone-mounted fruit recognition algorithm and harvesting mechanism for automatic fruit harvesting (자동 과일 수확을 위한 드론 탑재형 과일 인식 알고리즘 및 수확 메커니즘)

  • Joo, Kiyoung;Hwang, Bohyun;Lee, Sangmin;Kim, Byungkyu;Baek, Joong-Hwan
    • Journal of Aerospace System Engineering
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    • v.16 no.1
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    • pp.49-55
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    • 2022
  • The role of drones has been expanded to various fields such as agriculture, construction, and logistics. In particular, agriculture drones are emerging as an effective alternative to solve the problem of labor shortage and reduce the input cost. In this study therefore, we proposed the fruit recognition algorithm and harvesting mechanism for fruit harvesting drone system that can safely harvest fruits at high positions. In the fruit recognition algorithm, we employ "You-Only-Look-Once" which is a deep learning-based object detection algorithm and verify its feasibility by establishing a virtual simulation environment. In addition, we propose the fruit harvesting mechanism which can be operated by a single driving motor. The rotational motion of the motor is converted into a linear motion by the scotch yoke, and the opened gripper moves forward, grips a fruit and rotates it for harvesting. The feasibility of the proposed mechanism is verified by performing Multi-body dynamics analysis.

A Study on Bone Mineral Density, dietary habits and Nutritional Status of Adult Women in the three age groups (연령에 따른 성인여성의 골밀도와 식습관 및 영양섭취상태 연구)

  • Choi, Mi-Kyeong;Kim, Mi-Hyun
    • Journal of the Korean Society of Food Culture
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    • v.22 no.6
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    • pp.833-840
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    • 2007
  • The purpose of this study was to obtain baseline data of nutritional management for women's bone health according to age. This study was conducted through questionnaire, bone mineral density(BMD) measurement by DEXA and dietary survey by 24hr recalls to 134 adult women. Subjects were divided into three groups: young women group who aged 20-29(n=48), middle aged women group who aged over 30 and were pre-menopause(n=36), postmenopausal women group(n=48). The body fat percent of postmenopausal women group was the highest among the three groups. BMD of lumbar spine(L1-L2) were $0.93\;g/cm^2$ for young women, $0.97\;g/cm^2$ for middle aged women, and $0.88\;g/cm^2$ for postmenopausal women, respectively(p<0.05). BMD of femoral neck(p<0.05), trochanter(p<0.05), and ward's (p<0.001) were significantly decreased as age increasement. Drinking alcohol and skipping meals were significantly higher in young women groups than in the other groups, however frequency of doing regular exercise was significantly lower in young women groups. Young women group consumed the significantly higher amount of cereals(p<0.05), sugar and sweeteners(p<0.001), meats(p<0.01), eggs(p<0.01), milks(p<0.05) and oils(p<0.001) than middle aged and postmenopausal women. Also, energy(p<0.001), animal protein, (p<0.01), plant oil(p<0.001), animal fat(p<0.001), retinol(p<0.001), vitamin E(p<0.01), and cholesterol(p<0.001) intakes of young women were highest among the three groups. However, young women group consumed the significantly lower amount of dietary fiber(p<0.05), vitamin C(p<0.01), folate(p<0.05) and fruits(p<0.01) than the other groups. To summarized the our results, young women who were in still undergoing bone formation activity to develope peak bone mass, had more dietary habit and lifestyle problems than middle aged and postmenopausal women. The results of this study revealed that nutritional management and education for bone health should be emphasized in young women not only aged women.