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Report of seven unrecorded bacterial species in Korea belonging to the family Acetobacteraceae

  • Jun Heo;Hyorim Choi;Seunghwan Kim;Yiseul Kim;Daseul Lee;Byeong-Hak Han;Seung-Beom Hong;Soon-Wo Kwon
    • Journal of Species Research
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    • v.12 no.spc2
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    • pp.7-14
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    • 2023
  • Several genera belonging to the family Acetobacteraceae were generally considered to be acetic acid bacteria (AAB) which produce acetic acid from alcohols using an oxidation pathway. Some species of the family Acetobacteraceae have been of interest to the industry due to their capability to produce vinegar. In 2018-2020, several bacterial strains were isolated from plants, fruits and vinegar in various regions of the Republic of Korea. Based on the 16S rRNA gene sequences, seven species classified into the family Acetobacteraceae were found to be ones unrecorded in the Republic of Korea, including two Asaia species, one Gluconacetobacter species, three Gluconobacter species and one Komagataeibacter species. As a result, we report Asaia lannensis AF11C3 (=KACC 22050) isolated from plant Chrysanthemum zawadskii, Asaia platycodi AF15C2 (=KACC 22051) isolated from plant Isodon inflexus, Gluconacetobacter liquefaciens C23-3 (=KACC 22064) isolated from fruit of Pyrus pyrifolia, Gluconobacter cerinus BGF2-R2 (=KACC 22053) isolated from fruit of Prunus armeniaca, Gluconobacter kondonii FR39A4 (=KACC 22388) isolated from persimmon fruit, Gluconobacter thailandicus FR36C4 (=KACC 22057) isolated from fruit of Pseudocydonia sinensis and Komagataeibacter melaceti SPV-2 (=KACC 22058) isolated from potato vinegar.

Growth Characteristics of Strawberry and Kidney Bean Companion Planting in Building-integrated Urban Agriculture (건축물 일체형 도시농업에서 딸기와 강낭콩 공영식재에 따른 생육 특성)

  • Hyeon A Lee;Sun Yeong Lee;Yong Han Yoon;Jin Hee Ju
    • Journal of Environmental Science International
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    • v.32 no.12
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    • pp.955-964
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    • 2023
  • This study was conducted to obtain basic data on efficient and eco-friendly crop cultivation for urban residents who enjoy urban agriculture as a hobby or leisure activity. We planted strawberry(Fragaria x ananassa)(S) and kidney bean (Phaseolus vulgaris var. humilis)(K) in different ratios to analyze the differences in soil environment, growth, physiology, and productivity, and to investigate the effects. Strawberry growth was optimal with S1K2 ratio, whereas the S1K1 ratio treatment showed the highest levels of physiology and productivity. In terms of growth and physiology, kidney beans tended to perform best in S1K2 treatment. The average number of productive pods was two, with a highest average value of 2.3 being recorded in S2K1 treatment planted with a high percentage of strawberries. In terms of growth, physiology, and prodctivity our findings indicate that it would be desirable to plant starwberries and kidney bean in a 1:1 ratio. However, considering the environmental characteristics of walls and rooftops, it is necessary to effectively manage crops suitable for these conditions. Furthermore, additional studies should be conducted to analyze the quality of fruits and seeds producted, both qualitatively and quantitatively in the future.

β-Carotene, Cucumisin Content and Fruit Morphology of Melon (Cucumis melo) Germplasm Collection

  • Hyungjun Noh;Ae-Jin Hwang;Jae-Jong Noh;Bum-Soo Hahn;On-Sook Hur;Na-Young Ro;Jae-Eun Lee;Bit-Sam Kim;Ju-hee Rhee;Jung Yoon Lee;Ji Hyun Kim;Awraris Debie Assefa;Tania Afroz;Myung-kon Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.48-48
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    • 2021
  • This study was carried out to investigating quality and nutritional traits of melon genetic resources. It could provide important baseline data in breeding for increased β-Carotene, cucumisin content thereby increasing the marketability of melon. To this end, we have examined some fruit morphological traits and β-Carotene, cucumisin content of 163 genetic resources. The morphological characters were recorded on the field and inside laboratory and nutritional contents for β-carotene, cucumisin was measured using spectrophotomertic and HPLC methods. Melon fruits have shown a diverse morphological characters. Green and white is dominant for fruit skin color and round and oval-shape is dominant for form in the entire collections. The β-carotene content varied between 0.5 and 233.6mg/kg, 0.7 and 226.5mg/kg, 0.4 and 189.0 mg/kg using UV-Vis and microplated reader instruments, HPLC respectively. The high β-carotene contents were characterized five melon fruit originated from South Africa, Uzbekistan, Albania, France. The cucumisin content varied between 0.3 and 33.5 unit/mg, 0.29 and 32.1unit/mg using UV-Vis and microplated reader instruments, respectively.

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A comparative analysis of characteristics and antioxidant capacity of Korean mulberries for efficient seedling cultivation

  • Chan Young Jeong;Heon Woong Kim;Seong Ryul Kim;Hyun-Bok Kim;Kee-Young Kim;Seong-Wan Kim;Jong Woo Park;Ik Seob Cha;Sang Kug Kang;Ji Hae Lee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.47 no.2
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    • pp.147-154
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    • 2023
  • Mulberry exhibits unique characteristics and functionalities across various components, including the roots, branches, leaves, and fruits. However, despite numerous studies on mulberry, research on this plant at the seedling stage is insufficient. Therefore, this study aimed to compare the suitability for seedling cultivation and antioxidant effects of four Korean mulberry cultivars, namely, Daesim, Suhong, Simgang, and Cheongsu. In terms of seed weight, germination rate, and growth rate, Daesim was the most suitable cultivar for seedling production. Polyphenol and flavonoid content analysis showed that all cultivars, except for Cheongsu, showed the highest phenolic content at the 2-week seedling stage. Similarly, antioxidant assays using 2,2'-azino-di-3-ethylbenzthiazoline sulfonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals revealed that the antioxidant effect of all cultivars, except for Cheongsu, increased with cultivation at 2- 4- and 6-week. However, mulberry seedlings had a slower reaction rate against DPPH radical removal than mulberry leaves. In addition, ABTS radical scavenging activity showed a different correlation with polyphenol content. This phenomenon may be due to the different polyphenol compositions between mulberry leaves and seedlings. The results of this study suggest that mulberry seedlings exhibit different bioactivities from mulberry leaves, and component analysis is required in further research.

Effects of lemon or cinnamon essential oil vapor on physicochemical properties of strawberries during storage

  • Elise Freche;John Gieng;Giselle Pignotti;Salam A. Ibrahim;Helen P. Tran;Dong U. Ahn;Xi Feng
    • Food Science and Preservation
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    • v.30 no.4
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    • pp.549-561
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    • 2023
  • Recently, consumers have gained an interest in natural and minimally processed foods, inciting the food industry to consider using of natural products as preservatives. Strawberries are a widely consumed fruit but are also highly perishable. Therefore, in this study, the physicochemical properties of strawberries (Fragaria×ananassa) were evaluated after a 12-h treatment with lemon essential oil (Citrus×limon) or cinnamon essential oil (Cinnamomum cassia) vapor during storage at 22℃ for 4 days in an accelerated shelf-life study and 4℃ for 18 days in a validation study. Weight loss was blunted in fruit treated with oil vapor during the first days of storage (p<0.05). Lemon essential oil delayed fruit darkening (p<0.05) but reduced the firmness of strawberries (p<0.05). Strawberries treated with cinnamon essential oil had a higher concentration of reducing sugars (p<0.05), and a decrease of 16.7% visible decay, although the difference was insignificant. Oil vapor treatment did not alter the pH, organic acid content, or soluble solid content during storage compared to the control. Since lemon and cinnamon essential oils have well-documented antimicrobial properties, they may be suitable for the natural preservation of fruit. This study provides new information on using essential oil vapor treatment to preserve fruits, and potentially decrease fruit loss and waste.

Functional Composition and Antioxidant Activity from the Fruits of Rubus coreanus according to Cultivars (복분자 딸기 열매의 품종별 기능성 성분 및 항산화 활성)

  • Park, Youngki;Choi, Sun-Ha;Kim, Sea-Hyun;Jang, Yong-Seok;Han, Jingyu;Chung, Hun-Gwan
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.1
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    • pp.102-109
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    • 2008
  • To compare varietal differences in Rubus coreanus Miq. (Rosaceae), we evaluated antioxidant activity and the contents of functional substances. In this study, five new cultivars selected as superior berries in yield and weight of fruit, R. coreanus (Jungkeuam 1, 2, 3, 4, and 5), and one foreign berry, R. fructicosus were used. The contents of the major anthocyanin, cyanidin-3-glucoside (C3G) in berries and antioxidant capacity including, free radical scavenging activity and reducing power were measured. The contents of total phenol and vitamin C were also investigated. Among 5 cultivars and one foreign berry, Jungkeuam 1 had the richest C3G and high levels in vitamin C. DPPH radical scavenging activity of berries was ranged from 46.58 up to 78.55%.

Effect of Postharvest Treatments on Fruit Quality of 'Hong Bak' Peach during Shelf Life (복숭아(Prunus persica Batsch) '홍백'의 유통 중 품질에 미치는 수확 후 처리 효과)

  • Seo, Jeong-Seok;Hwang, Yong-Soo;Chun, Jong-Pil
    • Korean Journal of Agricultural Science
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    • v.36 no.2
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    • pp.147-158
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    • 2009
  • This study was conducted to investigate the effect of 1-MCP, market temperature and combination treatment of freshing agent with 1-MCP on fruit quality of 'Hong Bak' peach (Prunus persica Batsch). The 1-MCP treatment at the rate of 1000ppb showed the best results in delaying the firmness loss and skin color development during shelf life without any detrimental effects on fruit quality. Moreover, positive effects of 1-MCP on fruit quality parameters were more significant in the fruits stored at $30^{\circ}C$ of high temperature shelf life than those of $20^{\circ}C$. The combination treatment of carbon ceramic with 1-MCP showed a beneficial effects including a prevention of firmness loss and maintaining external appearance during the five days of room temperature storage of 'Hong Bak' peach.

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Hybridization Using a New Male-sterile Germplasm as the Female Parent in Chinese Jujube

  • Wang, Jiu-rui;Cui, Xiu-mei;Dai, Li;Liu, Ping;Zhao, Jin;Liu, Meng-jun
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.396-402
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    • 2015
  • This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 ${\times}$ 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 ${\times}$ 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.

Soil Physico-chemical Properties of Organic Grapes Farms with Different Culture Facilities and Soil Management Practices

  • Kim, Sun-Kook;Kim, Byeong-Sam;Kang, Beom-Ryong;Yang, Seung-Koo;Kim, Byeong-Ho;Kim, Hee-Kwon;Kim, Hyun-Woo;Choi, Kyeong-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.5
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    • pp.399-407
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    • 2013
  • Organic grape was generally produced in rainshield or plastic greenhouse culture while most of fruits were produced in open field. But little attention has been given to soil properties with different culture facilities in organic grape cultivation. This study was conducted to investigate soil physico-chemical properties of organic grapes farms with different culture facilities and soil management practices. Organic fertilizer was main resource to manage soil at organic grapes farms. Organic grapes farms were applied with total amount of organic fertilizer at one time, either at basal or additional fertilization, whereas conventional grapes farms applied with split fertilization. Bulk density and penetration resistance of soil were lower at both rainshield and green manure-applied plastic greenhouse cultures than those at clean plastic greenhouse culture. Especially, in plastic greenhouse, sod culture with natural weed after green manure application was more effective than general sod culture in improving physical properties of the rhizosphere. The contents of organic matter, available phosphate and exchangeable potassium tended to increase in the soils applied with green manure, and the difference of soil chemical properties were significant between rainshield and plastic greenhouse cultures. The optimum soil management was required in plastic greenhouse because pH, available phosphate and exchangeable cations reached over optimum range. Consequently, the ground cover management is the key factor to affect the chemical properties as well as soil physical properties extensively in plastic greenhouse. It is found that sod culture with natural weed after green manure application resulted in enhancement of utilization efficiency of nitrogen, phosphoric acid and potassium in soil in comparison with general sod culture.

Induction of p53-dependent Apoptosis by Resveratrol in Human Cancer Cells, A549 and SKOV3 (레스베라트롤에 의한 인간 암세포주, A549와 SKOV3의 p53의존적 Apoptosis 유발)

  • Lee, Seul Gi;Nam, Ju-Ock
    • Microbiology and Biotechnology Letters
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    • v.44 no.2
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    • pp.194-200
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    • 2016
  • Resveratrol, a polyphenolic compound present in many fruits and vegetables such as grapes, mulberries, and peanuts, has been reported to have various biological effects. However, the molecular mechanisms underlying resveratrol-induced apoptosis in A549 ovarian cancer cells are not well understood. In this study, we investigated the effect of resveratrol on A549 lung cancer cells (expressing wild-type p53) and compared it with that observed for SKOV3 ovarian cancer cells (expressing null-type p53). Resveratrol significantly inhibited the viability and proliferation of A549 cells in a concentration- and time-dependent manner, compared with its effects on SKOV3 cells. It also induced A549 cell apoptosis, but did not affect anoikis resistance. Furthermore, the viability and proliferation of p53-knockdown A549 cells were unaffected by the presence of resveratrol. Therefore, we demonstrate that the anticancer effect of resveratrol against A549 lung cancer cells is dependent on the presence of functional p53.