• Journal of the East Asian Society of Dietary Life
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• v.15 no.1
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• pp.100-105
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• 2005

This study was conducted to investigate the extraction characteristics of the polysaccharide from Jeseng mushroom (Fomitopsis pinicola Jeseng). Yields of the polysaccharide extracted from powdered mushroom by autoclaving(120, 30 min) with water at different pH and salt concentration were 8.2～9.2% in pH 5～11, 4.7～5.5% in 1～5% salt solution, respectively. The yield by the 0.05～1.0 N KOH-extraction was ranged 3.45～13.20%, while that by HAS-extraction(homogenizing after KOH swelling) using 1～2.5 N KOH 73.6～78.4%. Content of carbohydrate, protein, lipid and ash of the crude polysaccharide extracted from fruits body and its cultured mycelium by method of water extraction, KOH extraction(0.005～1N) and HAS-extraction were ranged 86.5～92.6%, 2.3～13.1%, 0.1～4.2% and 0.1～1.7%, respectively. The polysaccharide were composed of 62.0～77.8 g/g of pentose, 138.0～187.8 g/g of hexose and 21.2～117.3 mg/g of protein. From these results, the polysaccharide extracted was supposed to be a protein-bound polysaccharide.

• Applied Biological Chemistry
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• v.32 no.4
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• pp.378-385
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• 1989

Water-soluble crude polysaccharide (CAP-0) obtained from the Capsici Fructus(the fruits of Capsium annuum L.) showed a potent anti-complementary activity. The anti-complementary activity did not change by pronase digestion of CAP-0, but decreased by the periodate oxidation. CAP-0 was fractionated into four polysaccharide fractions, CAP-1,2,3 and 4, by the addition of cetyltrimethylammonium bromide. CAP-1, showing the highest anti-complementary activity, was refractioned by anion-exchange chromatography to give three major fractions(CAP-1-III, IV and V). CAP-1-III was shown to have low anti-complementary activity, but CAP-1-IV and V had high activity. CAP-1-III and IV were purified on Sephadex G-100 to give each two fractions(CAP-1-IIIa and IIIb, CAP-1-IVa and IVb), respectively. From the results of gel filtration and electrophoresis, these four fractions and CAP-1-V were found to be homogeneous polysaccharides. High molecular polysaccharies(M.W. CAP-1-IIIa 70,000, IVa 195,000, V 140,000) showed relatively higher anti-complementary activity than low molecular polysaccharides(CAP-1-IIIb and IVb).

• The Korean Journal of Food And Nutrition
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• v.27 no.6
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• pp.1147-1155
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• 2014

Adolescence is the most important period of healthy development. The purpose of this study was to evaluate the food recognition, snack preference, and dietary behavior of 1st grade of middle and high school boys and girls. Subjects were 5,554 students from 16 schools with healthy tuck shop and 3,406 students from 9 schools without healthy tuck shop in Seoul, Korea. Students from schools with healthy tuck shop are significantly higher than control group for facility satisfaction and hygiene satisfaction (all p<0.05). For fruit, the preference (p<0.05) and recognition (attitude, p<0.001: intention, p<0.05: eating habit, p<0.001: social-environment, p<0.001: self-efficacy, p<0.001) of students in schools with healthy tuck shop are significantly higher than those in schools without healthy tuck shop. For the habit of checking the manufacturer, students in schools with healthy tuck shop were significantly higher than students in schools without healthy tuck shop (p<0.05). The result suggested that we have to create an environment in which fruits can be purchased easily at a tuck shop and to educate adolescents for the importance of healthy food purchasing behavior. In conclusion, healthy school tuck shop had a positive effect on accessibility to healthy food.

• Korean journal of food and cookery science
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• v.26 no.5
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• pp.596-602
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• 2010

The objective of this study was to investigate parents' behavior and attitudes regarding their kids' snacking and dietary life education. A survey was conducted with the parents (N=412) of elementary students from seven provinces, includeing Seoul, Busan, Daegu, Kwangju, Gyungnam, Chungnam, and Kangwon. Kids' snacks consisted mainly of purchased foods rather than homemade foods. Parents' main concerns regarding kids' snacks were nutrition (44.9%), taste (24.3%), and safety (23.5%). Over 50% of parents checked food labels, especially expiration date (96.4%), food ingredients (49.5%), and food additives (33.5%). The snack items frequently provided to kids were fruits (62.8%), milk (55.5%), bread (47.1%), and snack items (23.3%). Parents responded that the amount of their kids' snacking was adequate (47.8%), small (33.7%), or large (18.2%). The results show that more research is needed regarding the adequacy of kids' snacking, considering that 15.7% and 22.4% of the children ate more and less, respectively, than their daily energy requirement. 76.5% of the parents performed home education regarding their kids' dietary habits, especially on the topics of 'balanced diet' (83.9%), 'healthful food' (53.7%), and 'eating three meals a day' (40.2%). Parents obtained dietary information from TV (65.2%), internet (12.0%), and newspapers (7.0%). From this study, we found that parents perceived their kids' snacking behavior correctly, and considered home-education for their kids' dietary habits positively. However, the information resources were limited, which makes it necessary to develop parents' education programs.

• Korean journal of food and cookery science
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• v.29 no.4
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• pp.399-406
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• 2013

The purpose of this study was to investigate eating habits, healthy eating behaviors, food preference etc. of university students for them to enjoy delightful and healthy campus life and offer preliminary data to the related educational facilities and households for them to make use of the data for making out a menu. We conducted survey aiming at 4-year-course students in Jeonbuk area and survey period was from May 6th of 2013 to May 24th of 2013. The result are as followings. First, food habit evaluation with 10 questions shows that they are inclined to have refreshments, water, vegetables, fruits, grains and protein food often and also eat food blandly. So, we consider this finding is generally positive. Second, through the research on their healthy eating behaviors, we learned their interests in healthy food is average and their selection criteria for healthy food is nutrition value. Information sources for healthy food are mainly TV or mass media and good healthy food in their opinion is Korean food. Third, the findings of food preference show university students like the white rice most but don't like the rice with beans most in terms of rice type. We also found their favorite food is fried rice, favorite noodle is spaghetti, favorite soups are thick beef soups, favorite broth is potato and pork rib broth, favorite stew is kimchi stew, favorite steamed dish is braised short ribs and favorite meat is pork.

• The Plant Pathology Journal
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• v.26 no.2
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• pp.139-148
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• 2010

A virus causing stunt, yellowing, severe mosaic, malformation symptoms on leaves and uneven development and malformation on fruits of zucchini was prevalent around Goseong, Gyeongsangnam-do, Korea. A survey conducted (2004) in the Goseong area revealed about 20% virus infection rate. The disease causative identified as Cucumber mosaic virus (CMV-Z1) was further characterized. The isolate induces mosaic symptoms on Cucumis sativus, while severe mosaic, stunt and malformation on C. pepo. Thin section analyses have shown that virus inclusions are formed in the cuticle layers as well as epidermal, parenchyma and collenchymas cells in virus-infected Nicotiana tabacum. CMV-Z1 isolate induced specific cytoplasmic inclusion bodies such as irregular clumps (IC), crystal (Cr) and irregular chloroplasts (ICh). IC was made up of virus particles interspersed with a darkly stained amorphous material and found both in the cytoplasm and vacuoles, whereas ICh and Cr were rarely found in the vacuoles. The genome of CMV-Z1 RNA-1 consists of 3359 nucleotide (nt) encoding 1a protein of 993 amino acids (aa). The CMV-Z1 RNA-2 was 3050 nt in length containing 2a (857 aa) and 2b (110 aa), while RNA-3 encoding 3a movement protein (279 aa) and coat protein (218 aa) was 2215 nt in length. Phylogenetic analyses of nucleotide sequences of CMV-Z1 isolate appeared it is more closely related to subgroup IA than to subgroup IB or II.

• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.117-123
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• 1992

Twenty and 24 lines selected from Korean local cultivar, Kalmi and Punggak, respectively, were grown in the University experiment farm to select promising lines for recurrent parent in a backcross breeding program for incorporation of resistance to Phytophthora capsici and Xanthomonas campestris pv vesicatoria into the Korean local cultivars. All the selections of 'Kalmi' and 'Punggak' were relatively late in maturity and the range of the variation was wider in the selections of 'Kalmi' than in those of 'Punggak'. Many of the selections from 'Kalmi' were vigorous, high in sugar content, and tolerant to virus in the field. Many of the selections from 'Punggak' were bearing large fruits with thick pericarp and high milling percentage. Line 6, 9, 14, 18 of 'Kalmi' and line 14, 16, 18 of 'Punggak' were selected as promising lines for recurrent parent in the backcross program.

• Korean Journal of Community Nutrition
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• v.19 no.1
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• pp.41-50
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• 2014

As Korea is moving toward multicultural society, the number of Muslim, whose religion has a great influence on diet-related behaviors, is increasing in Korea. Since the number of immigrants from Indonesia ranks within top ten in Korea and Indonesia is one of the most representative Islamic countries in Asia, understanding the diet-related behaviors and food preferences of Indonesians is needed. This study was conducted to investigate diet-related behaviors, factors affecting the diet-related perceptions, and food preferences of the Indonesians. The subjects included 500 Indonesians dwelling in Jakarta, Indonesia. About ninety % of the subjects in this study were Muslims. They did not eat pork and dog meat for religious reason and they practiced fasting during Ramadan period. Indonesians avoided pineapple, durian, and hot foods during pregnancy and usually ate vegetables and fruits after birth, which seemed to be due to sociocultural influence. Among the subjects, 21.5% were obese and major health problems reported were hypertension and hyperlipidemia. Their preference for fried food items might have contributed to health problems. Subjects had meals mostly at home (2.3 times/day) and dined out occasionally (1.2 times/week) at Indonesian restaurants (56.6%) or family restaurants (21.0%). In conclusion, Indonesians had home-based and native food-oriented dietary life with strong religious and sociocultural influence on food choices. Further research on specific nutrient intake analysis is needed to better understand and to improve dietary life of Indonesians in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.72-75
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• 2018

This study was conducted to investigate changes in the functional compounds in bitter gourd (Momordica charantia L.). Four treatments were used to analyze vitamin C, momordicin, K, Mg, and Ca compounds in Dragon bitter gourd on the 7th, 14th, 21st, and 28th days after pollination. According to the number of days after pollination, level of chemicals containing vitamin C in bitter gourd appeared to decrease continually after pollination, and was the highest, being 74.7 mg/100 g on the 7th day. The level of momordicin, an anti-diabetic was significantly higher in the 7th-day product than that of the 14th day;, meanwhile, there was no significant difference in Ca compounds. These phenomena are considered to fasten the stages of harvest for effective use of functional ingredients in bitter gourd fruits.

• Korean Journal of Clinical Laboratory Science
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• v.43 no.4
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• pp.161-170
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• 2011

Quercetin is one of the most distributed flavonoids in the plant kingdom and occurs naturally in a wide range of fruits and vegetables. This study was undertaken to determine whether quercetin exerts beneficial effect against necrotic and apoptotic cell death induced by hydrogen peroxide ($H_2O2$) in intestinal cells using the human-derived cultured T84 colonic epithelial cell line. Necrotic cell death was induced by exposing cells to 0.5 mM $H_2O_2$ for 2 h and apoptosis was induced by incubating cells in normal culture medium for 18 h following exposure of cells to 0.5 mM $H_2O2$ for 2 h. Cell viability was evaluated by the trypan blue exclusion assay and apoptosis was assessed by Hoechst 33258 staining and flow cytometry. $H_2O_2$ induced necrotic cell death in a time and dose-dependent fashion. Both necrotic and apoptotic cell deaths were not prevented by the antioxidants N,N'-diphenyl-p-phenylenediamine(DPPD) and Trolox, whereas both cell deaths induced by the organic hydroperoxide t-butylhydroperoxide (tBHP) were prevented by DPPD, suggesting that $H_2O_2$ induces cell death through a lipid peroxidation-independent mechanism. $H_2O2$-induced necrotic death was prevented by deferoxamine and 3-aminobenzamide, while the apoptotic cell death was not affected by these agents. Quercetin prevented both necrotic and apoptotic cell deaths induced by $H_2O_2$ in a dose-dependent manner. $H_2O_2$ caused activation of poly (ADP-ribose) polmerase (PARP), which was inhibited by deferoxamine, 3-aminobenzamide, and quercetin, but not DPPD. These results indicate that quercetin inhibits both necroticand apoptotic deaths of T84 cells. The anti-necrotic effect of quercetin may be attributed to its iron chelator activity rather than a direct $H_2O_2$ scavenging capacity and antioxidant. The present study suggests that quercetin may play a therapeutic role in the treatment of human gastrointestinal diseases mediated by oxidants.