• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.169-176
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• 1999

The purposes of this study were to set up the method of the natural killer(NK) cell activity assay using the flow cytometer and to examine the characteristics and distribution of the NK cell during rat hepatocarcinogenesis. Forty five male 6 week-old specific pathogen free(SPF) Sprague-Dawley rats were randomly divided into three groups. Group I was the non-treated control and given normal diet and water. Group II was treated with diethylnitrosamine(DEN, 200mg/kg, i.p.) and partial hepatectomy. Group III was treated with DEN, partial hepatectomy and 0.05% phenobarbital sodium in water from 3 to 16 weeks. All animals were examined the morphology of the large granular lymphocyte(LGL), the LGL percent of the total lymphocytes and the LGL conjugation rate with YAC-1 cell in peripheral blood, spleen and liver. Moreover, activity of the LGL isolated from peripheral blood lymphocytes was determined using the flow cytometer. As results, LGL were observed in the peripheral blood, spleen and liver. LGL were observed the relatively faintly staining basophilic cytoplasm with granules, and eccentric, often kidney-shaped nuclei in Giemsa stain. Its size was $11{\sim}13{\mu}m$. LGL percentage of the isolated lymphocytes in peripheral blood, spleen and liver were 1.8~2.3%, 1.3~1.4% and 0.87~0.99%, respectively. LGL conjugation rate with YAC-1 cell was shown to be peripheral blood(9.3~10.3 %) > spleen(7.7~8.7%) > liver(5.6~7.0%). The activity of the LGL isolated from peripheral blood lymphocytes in Group I, II and III was 33.7%, 30.5% and 35.4%, respectively. However, all values were not significantly between groups.

• Korean Journal of Environmental Agriculture
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• v.23 no.4
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• pp.228-233
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• 2004

Nitrate removal was examined from May to October 2003 of a surface flow treatment wetland cell, which was a part of a treatment wetland system composed of four wetland cells and a distribution pond The system was established on rice paddy near the Kohung Estuarine Lake located in the southern part of the Korean Peninsula. Effluent from a secondary-level night soil treatment plant was funneled into the system. The investigated cell, 87 m in length and 14 m in width, was created in April 2003. An open water was designed at its center, which was equivalent to 10 percent of its total area. Cattails (Typha angustifolia) were transplanted from natural wetlands into the cell and their stems were cut at about 40cm height from their bottom ends. Average $25.0\;m^3/day$ of effluent from the treatment plant was funneled into the cell by gravity flow and average $24.1\;m^3/day$ of its treated effluent was discharged into the Sinyang Stream flowing into the lake. Its water depth was maintained about 0.2 m and its hydraulic detention time averaged 5.2 days. Average height of the cattail stems was 42.5 cm in May 2M3 and 117.7 cm in September 2003. The number of stems averaged $9.5\;stems/m^2$ in May 2003 and $16.4\;stems/m^2$ in September 2003. The growth of cattails was good. Temperature of influent and effluent averaged 25.9 and $26.7^{\circ}C$, respectively. $NO_3$-N loading rate of influent and effluent averaged 176.67 and $88.09\;mg/m^2\;day$, respectively. Removal of rf03-N averaged $89.58\;mg/m^2\;day$ and its removal rate by mass was about 50%. Considering its initial operating stage in which cattail rhizomes and litter layer on the bottom were not Idly established, the $NO_3$-N removal rate of the cell was rather good.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.327-339
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• 2003

During the winter in February 1998, January and April 1999, interdisciplinary research was conducted in a large area including the South Sea of Korea and northern East China Sea to examine distribution and structure. Water masses identified from the observed data are Warm Water originated from Tsushima Warm Current, Yellow Sea Cold Water (Northern or Central Cold Water) and Korean Southern Sea Cold Water. In the southern Yellow Sea, Warm Water originated from Tsushima Warm Current, flowing into the Cheju Strait after turning around the western Cheju Island, makes a front of '┍' shape, which is bounded by the Yellow Sea Central Cold Water in the southern part of Daeheuksan Island and by the Yellow Sea Northern Cold Water in the eastern part of the Yangtze Bank. This front changes its corner shape and position with strength of the warm water extension toward northwestern Yellow Sea. The position and structure of the fronts off the southwestern tip of the Korean peninsular and near the Yangtze Bank varies with observation period. In the front in the South Sea of Korea, cold coastal water which if formed independently due to local cooling, ,sinks along the sloping bottom. We explained the processes of variations in the distribution and structure of these winter fronts in terms of up-wind and down-wind flow by the seasonal monsoon, heat budget through the sea surface and density difference across the fronts.

• Tunnel and Underground Space
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• v.14 no.4
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• pp.287-294
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• 2004

To predict the temperature distribution around a underground rock storage cavern, two- and three- dimensional numerical analysis using FLAC was conducted. The effects of groundwater and latent heat on thermal properties were considered in numerical calculation. The temperature estimated by FLAC are compared with the temperature measured for 5-year operation at Gonjiam storage cavern. Estimated and measured temperatures showed great discrepancy when thermal properties from laboratory tests were used and showed good agreement when the effects from 20% of volumetric water fraction and latent heat were considered. However, the discrepancy still increased with operation time due to the heat flow from ground surface. Three-dimensional numerical models were established to closely approximate the boundary condition of the test site, and numerical results better agreement when groundwater and latent heat effects were considered.

• Journal of Korean Society of Disaster and Security
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• v.14 no.4
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• pp.47-60
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• 2021

Recently, as the occurrence frequency of sudden floods due to climate variability increased, the damage of aging chuteway slabs of spillway are on the rise. Accordingly, a wide array of field survey, hydraulic experiment and numerical simulation have been conducted to find the cause of damage on chuteway slabs. However, these studies generally reviewed the flow characteristics and distribution of pressure on chuteway slabs. Therefore the derivation of damage on chuteway slabs was relatively insufficient in the literature. In this study, the cavitation erosion and hydraulic jacking were assumed to be the causes of damage on chuteway slabs, and the phenomena were reproduced using 3D numerical models, FLOW-3D and COMSOL Multiphysics. In addition, the cavitation index was calculated and the von Mises stress by uplift pressure distribution was compared with tensile and bending strength of concrete to evaluate the possibility of cavitation erosion and hydraulic jacking. As a result of numerical simulation on cavitation erosion and hydraulic jacking under various flow conditions with complete opening gate, the cavitation index in the downstream of spillway was less than 0.3, and the von Mises stress on concrete was 4.6 to 5.0 MPa. When von Mises stress was compared with tensile and bending strength of concrete, the fatigue failure caused by continuous pressure fluctuation occurred on chuteway slabs. Therefore, the cavitation erosion and hydraulic jacking caused by high speed flow were one of the main causes of damage to the chuteway slabs in spillway. However, this study has limitations in that the various shape conditions of damage(cavity and crack) and flow conditions were not considered and Fluid-Structure Interaction (FSI) was not simulated. If these limitations are supplemented and reviewed, it is expected to derive more efficient utilization of the maintenance plan on spillway in the future.

• Journal of odor and indoor environment
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• v.17 no.4
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• pp.355-361
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• 2018

Mold grows more easily when humidity is higher in indoor spaces, and as such is found more often on wetted areas in housing such as walls, toilets, kitchens, and poorly managed spaces. However, there have been few studies that have specifically assessed the level of mold in the indoor spaces of water-damaged housing in the Republic of Korea. We investigated the levels of airborne mold according to the characteristics of water damage types and explored the correlation between the distribution of mold genera and the characteristics of households. Samplings were performed from January 2016 to June 2018 in 97 housing units with water leakage or condensation, or a history of flooding, and in 61 general housing units in the metropolitan and Busan area, respectively. Airborne mold was collected on MEA (Malt extract agar) at flow rate of 100 L/min for 1 min. After collection, the samples were incubated at $25^{\circ}C$ for 120 hours. The cultured samples were counted and corrected using a positive hole conversion table. The samples were then analyzed by single colony culture, DNA extraction, gene amplification, and sequencing. By type of housing, concentrations of airborne mold were highest in flooded housing, followed by water-leaked or highly condensed housings, and then general housing. In more than 50% of water-damaged housing, the level of airborne mold exceeded the guideline of Korea's Ministry of Environment ($500CFU/m^3$). Of particular concern was the fact that the I/O ratio of water-damaged housing was greater than 1, which could indicate that mold damage may occur indoors. The distribution patterns of the fungal species were as follows: Penicillium spp., Cladosporium spp. (14%), Aspergillus spp. (13%) and Alternaria spp. (3%), but significant differences of their levels in indoor spaces were not found. Our findings indicate that high levels of mold damage were found in housing with water damage, and Aspergillus flavus and Penicillium brevicompactum were more dominant in housing with high water activity. Comprehensive management of flooded or water-damaged housing is necessary to reduce fungal exposure.

• Journal of the Korean Society of Propulsion Engineers
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• v.3 no.1
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• pp.9-14
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• 1999

The effect of operating condition was studied experimently on the characteristics of twin sprays ejected from two airblast atomizers, within the range of the mass air-fuel ratio 1.36∼3.54. Water and nitrogen gas were used as test fluids for the experiments. Spray characteristics of liquid spray were measured with measurement of mass distribution and instantaneous image of the spray cone. Experimental results show that the maximum specify of the distribution were lowered but distributed over the larger area when the ROA ratio increased, Center of mass position did not change with increasing water mass flow, Increase of the nozzle distance has an small effect on mass distribution of interaction area but distributed over the larger area. It was also conformed that the effect of interaction near central point of collision decreased with the increase of the ROA ratio on interaction area from comparison using superposition method

• Water for future
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• v.29 no.2
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• pp.179-190
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• 1996

The characteristics of water quality in the downstream of the Han River were analyzed by statistical techniques. Basic characteristics, areal and temporal variations, and correlations of water quality data were investigated. Monthly water quality data have been investigated systematically by exploring data analysis, including time series plot, summary statistics, distribution test, time dependence test, seasonality test and flow relatedness test. Results show that water quality data in this river have seasonality. And applicability of stochastic models such as Thomas-Fiering model and ARMA(1,1) model was identified. From the examination of water quality data related to discharge, it was found that DO and SS are sensitive to water temperature rather than discharge, while BOD and COD are sensitive to discharge at dry seasons. Seasonal periodicities were identified in all water quality variables from the cross correlation analysis.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.4
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• pp.421-428
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• 2005

Dissolved air flotation (OAF) is a solid-liquid separation system that uses fine bubbles rising from bottom to remove particles in water. In this study, we investigated the effect of L/W (L; Length, W; Width) on the hydro-dynamic behavior in DAF system using CFD (Computational Fluid Dynamics) and ADV (Acoustic Doppler Velocimetry) technique. The factual full-scale DAF system, L/W ratio of 1:1, was selected and various L/W ratio (2:1, 3:1, 4:1 and 5:1) conditions were simulated with CFD. For modelling, 2-phase (gas-liquid) flow equations for the conservation of mass, momentum and turbulence quantities were solved using an Eulerian-Eulerian approach based on the assumption that very small particle is applied in the DAF system. Also, for verification of CFD simulation results, we measured the factual velocity at some points in the full-scale DAF system with ADV technique. Both the simulation and the measurement results were in good accordance with each other. As the results of this study, we concluded that L/W ratio and outlet geometry play important role for flow pattern and fine bubble distribution in the flotation zone. In the ratio of 1:1, the dead zone is less than those in other cases. On the other hands, in the ration of 3:1, the fine bubbles were more evenly distributed.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.233-233
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• 2015

Vu Gia - Thu Bon basin is located in central Vietnam between Truong Son mountain range on the border with Lao in the west and the East Sea in the east. The basin occupies about 10,350 km2 or roughly 90% of the Quang Nam Province and includes Da Nang, a very large city with about 876,000 inhabitants. Total annual rainfall ranges from about 2,000 mm in central and downstream areas to more than 4,000 mm in southern mountainous areas. Rainfall during the monsoon season accounts for 65 to 80% of total annual rainfall. The highest amount of rainfall occurs in October and November which accounts for 40 to 50% of the annual rainfall. Rainfall in the dry season represents about 20 to 35% of the total annual rainfall. The low rainfall season usually occurs from February to April, accounting for only 3 to 5% of the total annual rainfall. The mean annual flow volume in the basin is $19.1{\times}109m 3$. Similar to the distribution of rainfall, annual flows are distinguished by two distinct seasons (the flood season and the low-flow season). The flood season commonly starts in the mid-September and ends in early January. Flows during the flood season account for 62 to 69% of the total annual water volume, while flows in the dry season comprise 22 to 38% of total annual run-off. The water volume gauged in November, the highest flow month, accounts for 26 to 31% of the total annual run-off while the driest period is April with flows of 2 to 3% of the total annual run-off. There are some hydropower projects in the Vu Gia - Thu Bon basin as the cascade of Song Bung 2, Song Bung 4, and Song Bung 5, the A Vuong project currently under construction, the Dak Mi 1 and Dak Mi 4 projects on the Khai tributary, and the Song Con project on the Con River. Both the Khai tributary and the Song Con join the Bung River downstream of SB5, although the Dak Mi 4 project involves an inter-basin diversion to Thu Bon. Much attention has recently been focused on the effects that climate variability and human activities have had on runoff. In this study, data from the Vu Gia - Thu Bon River Basin in the central of Viet Nam were analyzed to investigate changes in annual runoff during the period of 1977-2010. The nonparametric Mann-Kendall test and the Mann-Kendall-Sneyers test were used to identify trend and step change point in the annual runoff. It was found that the basin had a significant increasing trend in annual runoff. The hydrologic sensitivity analysis method was employed to evaluate the effects of climate variability and human activities on mean annual runoff for the human-induced period based on precipitation and potential evapotranspiration. This study quantitatively distinguishes the effects between climate variability and human activities on runoff, which can do duty for a reference for regional water resources assessment and management.