• The Plant Pathology Journal
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• 제17권3호
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• pp.115-122
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• 2001

Potato virus X (PVX) is a flexuous rod-shaped virus containing a single plus-strand RNA. Viral RNA synthesis is precisely regulated by regulatory viral sequences and by viral and/or host proteins. RNA sequence element as well as stable RNA stem-loop structure in the 5' end of the genome affect accumulation of genomic RNA and subgenomic RNA (sgRNA). The putative sgRNA promoter regions upstream of the PVX triple gene block (TB) and coat protein (CP) gene were critical for both TB and CP sgRNA accumulation. Mutations that disrupted complementarity between a region at the 5' end of the genomic RNA and the sequences located upstream of each sgRNA initiation site is important for PVX RNA accumulation. Compensatory mutations that restore complementarity restored sgRNA accumulation levels. However, the extent of reductions in RNA levels did not directly correlate with the degree of complementarity, suggesting that the sequences of these elements are also important. Gel-retardation assays showed that the 5' end of the positive-strand RNA formed an RNA-protein complex with cellular proteins, suggesting possible involvement of cellular proteins for PVX replication. Future studies on cellular protein binding to the PVX RNA and their role in virus replication will bring a fresh understanding of PVX RNA replication.

• The Korean Journal of Physiology and Pharmacology
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• 제17권1호
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• pp.89-97
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• 2013

Developing an animal model for a specific disease is very important in the understanding of the underlying mechanism of the disease and allows testing of newly developed new drugs before human application. However, which of the plethora of experimental animal species to use in model development can be perplexing. Administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a very well known method to induce the symptoms of Parkinson's disease in mice. But, there is very limited information about the different sensitivities to MPTP among mouse strains. Here, we tested three different mouse strains (C57BL/6, Balb-C, and ICR) as a Parkinsonian model by repeated MPTP injections. In addition to behavioral analysis, endogenous levels of dopamine and tetrahydrobiopterin in mice brain regions, such as striatum, substantia nigra, and hippocampus were directly quantified by liquid chromatography-tandem mass spectrometry. Repeated administrations of MPTP significantly affected the moving distances and rearing frequencies in all three mouse strains. The endogenous dopamine concentrations and expression levels of tyrosine hydroxylase were significantly decreased after the repeated injections, but tetrahydrobiopterin did not change in analyzed brain regions. However, susceptibilities of the mice to MPTP were differed based on the degree of behavioral change, dopamine concentration in brain regions, and expression levels of tyrosine hydroxylase, with C57BL/6 and Balb-C mice being more sensitive to the dopaminergic neuronal toxicity of MPTP than ICR mice.

• Journal of Nutrition and Health
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• 제39권7호
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• pp.641-648
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• 2006

This study was designed to evaluate the effect of the individual nutrition education for type 2 diabetes who participate the diabetes buffet. The subjects were 66 patients and divided into education (n=34) and control groups (n=32). The mean age of education and control groups were 59.8 and 56.6 years old, respectively. There were no differences in age and body mass index (BMI) between two groups. Initial glycosylated hemoglobin (HbA1c), post prandial plasma glucose (PP2), total plasma cholesterol, and blood pressure were not different between two groups. But fasting blood sugar (FBS) was higher in education group than in control group. On completion of the study, the education group showed significant decreases in body weight, BMI, FBS and PP2, however, the control group showed no changes in body weight, BMI and PP2, and showed a significant increase in FBS. Initial calorie and protein intakes of the education group did not meet the prescribed amount, however, mineral and vitamin intakes were higher than estimated average requirement (EAR). By the end of study, calorie and protein intakes were significantly increased to meet the prescription. In order to evaluate the effect of diet education, awareness of calorie requirement was used as an index of understanding diet prescription. The degree of awareness of calorie requirement was dependent on age: younger patients showed higher awareness than older subjects. The subject who showed better understanding of diet prescription showed lower levels of HbA1c, FBS, and PP2 at the end of the experiment period. The results of this study clearly show that individual diabetes diet education is effective to make the patient understand their diet prescription, and is effective to control body weight and blood sugar level. Awareness of calorie requirement could be used as an index of understanding of prescribed diet. Since age is an important variable to determine the awareness of calorie requirement, different strategies of nutrition education should be developed for different age groups, especially patients over 70 years old.

• 한국환경교육학회지:환경교육
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• 제13권1호
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• pp.65-74
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• 2000

Environmentally literated students and adults should be able to use and apply the basic ecological concepts when considering environmental problems and issues. Because ecology forms the foundation of environmental education, a review of the literatures on the degree of students' understanding for ecological concepts can provide useful insights for environmental educators. The purposes of this study were as follows: first, to analyse the parts connected with ecological knowledge of two environmental education books, ‘environments’(middle school) and ‘environmental science’(high school), second, to investigate the level of understanding on ecological concepts by the use of new developed instrument. The 20 most important ecological concepts from Cherrett(1989) and the important ecological concepts' lists from Hungerford and Volk(1990), Ramsey, Hungerford and Volk(1992), Volk(1993) would be recognized and endorsed by most environmental educators as concepts essential to environmental literacy, We referenced these informations and sequenced ecological knowledge as four main categories(communities, populations, ecosystem, man as a component of the nature systems). We have used it as a criterion in the analysis of teaching materials and the development of a new test instrument($\alpha$=0.81). According to the analysed results, the understanding levels for scales and relationships of communities, populations, ecosystem were high but those for individual concepts and differences were low. By the analysis of Korean High School EE book, ‘environmental science’, learning for some concepts(succession, material cycling, niche etc.) has pointed out as one of problems. Environmental educators must pay a careful attention to the concepts that showed high rates of incorrect answer and this work will contribute toward consolidating the basis of EE and help the accomplishment of the ultimate goals in EE.

• 한국데이타베이스학회:학술대회논문집
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• 한국데이타베이스학회 2010년도 춘계국제학술대회
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• pp.5-12
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• 2010

With a sample of 147 Korean small and medium size companies, this study examined the relationships among degree of information orientation, corporate culture, degree of information management implementation and selected business performances in the process of implementing IMS improvement programs, such as IMS(Information Management System). Information orientation is defined as company-wide understanding and implementation of the underlying philosophy, principles, approached, and tools of information improvement programs. It is assumed that successful implementation of information improvement programs requires a information-oriented mind-set of the employees. The key elements of information orientation include continious improvement structured processes, organixation-wide participation and customer-focused spirit. Culture id defined as the value and beliefs of em organization that shape its behavior. It is also assumed that successful implementation of information improvement programs require strong support from s corporate culture that emphasizes cintinious improvement. Adopting the competing values model of Quinn and McGrath(1985), corporate culture is classified into 'flexible' versus 'controlled culture' and 'outer-directed' versus 'inner-directed culture'. Fitness was defined through the relationship between levels of information oriented and types of corporate culture. The results were as follows. First, it was found that when a company with high information orientation promoted information innovation programs, such as IMS, it reported higher degree of information management implementation and improvement in business performances. Second, the results showed the importance of 'flexible culture' and 'outer-directed culture' in performing information, innovation. Regarding the types of corporate culture, the analysis found that developmental culture, rational culture and group culture were effective. Third, companies with high information oriented and flexible culture or companies with high information orientation and outer-directed culture reported the highest implementation in Information management activities. Fourth, the results showed that the level of information management implementation had a mediating effect on the relationship between information orientation and business performance. It was also found that enhanced non-financial performance led to the improvement of financial performance. This study attempted to exaime the factor that lead information management program to success. In order to reach success, first, it is suggested that companies have positive mind set toward continious information improvement. Secondly, it is recommended that a flexible and outer-directed culture appropriate for continious information improvement is cultivated.

• 한국산학기술학회논문지
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• 제17권9호
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• pp.628-640
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• 2016

본 연구는 간호대학생의 감성지능이 진로결정 자기효능감과 진로결정수준에 미치는 영향요인을 파악하고자 실시된 서술적 조사연구이다. 대상자는 대구 경북지역 간호 대학생 3,4학년 223명이며, 구조화된 설문지를 이용하여 2015년 10월 19일부터 10월 30일까지 편의표집 하였다. 본 연구에서 수집된 자료는 SPSS 22.0 통계 프로그램을 이용하여, 인구사회학적 특성, 감성지능, 진로결정자기효능감, 진로결정수준은 기술통계(빈도, 백분율, 평균, 표준편차)를 실시하고, 측정도구의 신뢰도는 Cronbach's ${\alpha}$값을 구하였다. 일반적인 특성에 따른 감성지능, 진로결정자기효능감, 진로결정수준의 차이는 t-test와 ANOVA를 하였고, 사후검정은 Scheffe test를 실시하였다. 또한 주요변인 간의 상관관계는 Pearson's correlation coefficients를 산출하여 분석하였다. 대상자의 일반적 특성과 감성지능이 진로결정자기효능감과 진로결정수준에 미치는 영향을 살펴보기 위하여 다중회귀분석을 실시하였다. 분석결과 감성지능과 진로결정 자기효능감, 진로결정수준은 정(+)적 상관성을 보였다. 진로결정 자기효능감에 미치는 영향요인은 성별(${\beta}=-.155$), 취업률 동기(${\beta}=-.145$), 감성 활용(${\beta}=.236$), 감성조절(${\beta}=.197$), 타인 감성이해(${\beta}=.157$)가 유의미하였다. 진로결정수준에 미치는 영향요인은 학년(${\beta}=.273$), 취업률 동기(${\beta}=-.145$), 주위의 권유동기(${\beta}=-.138$), 감성조절(${\beta}=.223$)이 유의미하였다. 따라서 간호학과 선택 시 자신의 의지로 선택할 수 있도록 학교나 가정에서 간호사의 역할이나 임무 등에 대하여 충분하게 조언할 필요가 있다. 또한 학사과정에서 정서적 교육 등의 비중을 높이면 감성지능을 향상시킬 수 있을 것이다.

• 한국학교수학회논문집
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• 제11권3호
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• pp.513-541
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• 2008

본 연구의 목적은 8학년 학생들의 사각형 학습 및 기하학적 추론 능력의 발달을 위해서 GSP의 사용이 자와 각도기 같은 전통적인 도구의 사용보다 더 효과적인지를 탐구하고, 어떻게 그 소프트웨어의 사용이 학생들의 사각형 학습과 추론 능력의 발달에 영향을 끼치는지를 조사하는 것이다. 사후 학업 성취도 검사 결과에 의하면 GSP를 사용한 집단과 자와 각도기를 사용한 집단의 평균 성적에서 통계적으로 유의미한 차이가 발견되었다. GSP를 사용한 집단이 자와 각도기를 사용한 집단보다 유의미하게 높은 평균 성적을 보여주었다. 학생 면접 결과에 의하면, GSP의 사용이 학생들의 기하학적 추론 능력을 발달시키는데 더 효과적이었다. GSP를 사용한 집단의 학생들이 자와 각도기를 사용한 집단의 학생들보다 van Hiele 2와 3수준에서 더 높은 정도의 달성도를 보여주었다. GSP가 제공하는 수학적 개념에 대한 역동적인 시각적 효과와 조작 경험이 학생들이 사각형 학습을 개념적으로 접근하도록 하는데 중요한 역할을 하였고, 그런 경험들이 학생들이 기존에 갖고 있던 수학적 개념에 처한 오류를 확인하고 개념을 재정립하는데 도움을 주었다.

• Earthquakes and Structures
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• 제20권4호
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• pp.457-471
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• 2021

This study aims at providing a simple and effective methodology to define a meaningful characteristic period for special class of earthquake records named "pulse-like ground motions". In the proposed method, continuous wavelet transform is employed to extract the large pulse of ground motions. Then, Fourier amplitude spectra obtained from the original ground motion and the residual motion is simply compared. This comparison permits to define a threshold pulse-period (Tp∗) as the threshold period above which the pulse component has negligible contributions to the Fourier amplitude spectrum. The effect of pulse on frequency content of motions was discussed on the light of this definition. The advantage and superior features of the new definition were related to the inelastic displacement ratio (IDR) for single-degree-of-freedom systems with period equal to one half of the threshold period. Analyses performed for the proposed period at three ductility levels u=2,4,6 were compared with the results obtained at half of pulse period derived from wavelet analysis, peak-point method and the peak of product of the velocity and the displacement response spectra (Sv x Sd). According to the results, pulse effects on inelastic displacement ratio seem to be more important when $\frac{T_p^*}{T}=2$ (T is the fundamental vibration period of system). The results showed that utilizing of the proposed definition could facilitate an enhanced understanding of pulse-like records features.

• Genomics & Informatics
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• 제19권2호
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• pp.14.1-14.6
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• 2021

Coronavirus disease 2019 (COVID-19) is an on-going pandemic disease infecting millions of people across the globe. Recent reports of reduction in antibody levels and the re-emergence of the disease in recovered patients necessitated the understanding of the pandemic at the core level. The cases of multiple organ failures emphasized the consideration of different organ systems while managing the disease. The present study employed RNA sequencing data to determine the disease associated differentially regulated genes and their related protein interactions in several organ systems. It signified the importance of early diagnosis and treatment of the disease. A map of protein interactions of multiple organ systems was built and uncovered CAV1 and CTNNB1 as the top degree nodes. A core interactions sub-network was analyzed to identify different modules of functional significance. AR, CTNNB1, CAV1, and PIK3R1 proteins were unfolded as bridging nodes interconnecting different modules for the information flow across several pathways. The present study also highlighted some of the druggable targets to analyze in drug re-purposing strategies against the COVID-19 pandemic. Therefore, the protein interactions map and the modular interactions of the differentially regulated genes in the multiple organ systems would incline the scientists and researchers to investigate in novel therapeutics for the COVID-19 pandemic expeditiously.

• 한국초등과학교육학회지:초등과학교육
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• 제21권1호
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• pp.13-24
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• 2002

This study examined the knowledge structure constructed by children before formal instruction, and successive changes in the structural complexity of knowledge during the learning of the 'Structure and Function of Plants' unit. The researchers let the 5th graders draw the first draft of their concept maps in order to examine the pre-existing knowledge structure concerned with the unit and also four concept maps after completing every fourth lesson. Each concept map drawn by children on the basis of the previous one showed the degree of their current understanding on the structure and function of plants. The results revealed that only two levels of hierarchy and five relationships among the components of the first concept map(relationship, hierarchy, cross link and example) were proven to be valid in terms of conceptual relevance. According to the standard map, there was no change in hierarchy from the 2nd to the 3rd map, and in example from the 2nd to the 4th map. However, the gradual and successive increases of the scores in all components appeared in the children's maps throughout the unit. Knowledge restructuring occurred strongly in the early periods from the 1st to the 6th lesson, and the significant stable changes in tuning and accretion appeared throughout the whole lessons. The results also showed that there were no significant gender differences on the 5th grader's knowledge structuring.