• BMB Reports
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• 제36권5호
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• pp.493-498
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• 2003

The scFv antibody towards the Burkholderia pseudomallei exotoxin was previously constructed by phage display and exhibited good specificity towards the exotoxin. We report here the optimization of the scFv expression in an E. coli expression system. Four different E. coli strains (ER2537, TG1, HB2151, and XL1-Blue) were examined for optimal expression of the scFv protein. Two types of carbon source (i.e. 0.2% glucose and 0.2% glycerol) were also tested for their ability to induce the scFv expression. Cells that carried the scFv construct were grown at $30^{\circ}C$ and induced with 0.05 mM IPTG. The expression was then monitored by SDS-PAGE, Western blotting, and indirect ELISA. The Western blot profile showed different levels of the scFv expression among the host strains; XL1-Blue exhibited the highest level of the scFv protein expression. Glycerol at a concentration of 0.2% (v/v) significantly increased the scFv protein expression level when compared to 0.2% (w/v) glucose. Further optimization demonstrated that the scFv protein expression in XL1-Blue was the most optimal with a glycerol concentration as low as 0.05%. However, by indirect ELISA, only the scFv protein that was expressed in 0.2% (v/v) glycerol exhibited high specificity towards the Burkholderia pseudomallei exotoxin.

• Journal of Information Processing Systems
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• 제13권4호
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• pp.653-667
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• 2017

The significant advances in information and communication technologies are changing the process of how information is accessed. The internet is a very important source of information and it influences the development of other media. Furthermore, the growth of digital content is a big problem for academic digital libraries, so that similar tools can be applied in this scope to provide users with access to the information. Given the importance of this, we have reviewed and analyzed several proposals that improve the processes of disseminating information in these university digital libraries and that promote access to information of interest. These proposals manage to adapt a user's access to information according to his or her needs and preferences. As seen in the literature one of the techniques with the best results, is the application of recommender systems. These are tools whose objective is to evaluate and filter the vast amount of digital information that is accessible online in order to help users in their processes of accessing information. In particular, we are focused on the analysis of the fuzzy linguistic recommender systems (i.e., recommender systems that use fuzzy linguistic modeling tools to manage the user's preferences and the uncertainty of the system in a qualitative way). Thus, in this work, we analyzed some proposals based on fuzzy linguistic recommender systems to help researchers, students, and teachers access resources of interest and thus, improve and complement the services provided by academic digital libraries.

• 동의생리병리학회지
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• 제20권4호
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• pp.784-792
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• 2006

The Neo-Confucian study of the thee of ministerial fire(相火論) constitute of the rule of iung jung(中正), the principal of form and use(體用) and of real nature and emotion(性情) of human and ethic mind(人心道心). The present study is to evaluate a fundamental concept of the theory of ministerial fire, which is one of traditional medical ideology in China, through the Neo-Confucianism projects to The theory of ministerial fire. The theory of ministerial fire of Judange(朱丹溪) was recognized by ontological principal of Heaven-Human being-Earth, ontological structure of Form-Use and the structure that mind consist of real nature and emotion(심統性惰). The ethic and human mind(道心人心) of Judange and constancy and transition(常變) of seven emotion have relationship in the ontological structure of Form-Use. The real nature of human Doing consisted of apriority of Heaven rule is unitary constructed by Form-Use(체용) of inactivated real nature and activated seven emotion and then activated seven emotion shows dual form of appropriate(中節) and inappropriate(不中節). Emperor's and ministerial fire(君火相火) which has a relationships of Heaven-Human being synchronization represents all kinds of fire and classified to heaven fire(天火) and human fire(人火). The emperor's fire was triggered by inactivated fire and ministerial fire(相火) was triggered by activated fire. Inactivated ministerial fires have dual form of physiological ministerial and pathological ministerial fire. Regarding the forementioned analytic thinking, it was clear that manifest processing of One's real nature and the ministerial fire undergo the same principal and logics. Since maintained One's real nature that is inactivated seven emotion and appropriated activated seven emotion, ministerial fire can be stable and keep one's health and well-being in mind and body.

• 동양고전연구
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• 제33호
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• pp.7-29
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• 2008

장현광(張顯光)은 조선시대 자연학자 가운데 대표적인 인물 가운데 한 사람이다. 이 글은 그의 우주론 속에 함축된 상수학적 요소의 의의와 한계를 검토하는 데 목표를 둔다. 역학에서 상과 수는 만물의 분별과 그 상호관계, 그리고 그것들의 시간적 질서를 표현하는 개념 도구이다. 그것들은 음양을 결합함으로써 내부 운동인을 확보하고, 우주 만물에 대한 상수학적 해석과 설명의 틀을 구성하였다. 상수학적 우주론의 핵심적인 주장은 우주가 시 공간적으로 유한하며, 그것과 반대로 리(태극)는 무한하다고 하는 것이다. 리가 시 공간적 무한하다는 것은 도덕원리가 절대적이고 항상적임을 의미한다. 이를 통해 장현광은 자신의 우주론이 주자학의 도덕형이상학과 순조롭게 결합될 수 있도록 하였다. 장현광은 역서(易書)를 자연 이해의 준거 또는 범형(範形)으로 강조하는 경향을 보였다. 이는 불완전하나마 소옹에게서 나타났던 법칙 객관주의를 질곡하고, 그의 법칙 개념으로 하여금 경험이나 지식의 확장과 더불어 스스로를 갱신해 가기 어렵게 만들었다. 이러한 이론적 한계에도 불구하고 그의 상수학적 우주론은 당시 성리학의 자연학적 근거를 마련했다는 점에서 긍정적으로 평가할 수 있다.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.319-324
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• 2005

An integrated approach for prostate cancer detection using proteomic data is presented. Due to the high-dimensional feature of proteomic data, the discrete wavelet transform (DWT) is used in the first-stage for data reduction as well as noise removal. After the process of DWT, the dimensionality is reduced from 43,556 to 1,599. Thus, each sample of proteomic data can be represented by 1599 wavelet coefficients. In the second stage, a voting method is used to select a common set of wavelet coefficients for all samples together. This produces a 987-dimension subspace of wavelet coefficients. In the third stage, the Autoassociator algorithm reduces the dimensionality from 987 to 400. Finally, the artificial neural network (ANN) is applied on the 400-dimension space for prostate cancer detection. The integrated approach is examined on 9 categories of 2-class experiments, and also 3- and 4-class experiments. All of the experiments were run 10 times of ten-fold cross-validation (i. e. 10 partitions with 100 runs). For 9 categories of 2-class experiments, the average testing accuracies are between 81% and 96%, and the average testing accuracies of 3- and 4-way classifications are 85% and 84%, respectively. The integrated approach achieves exciting results for the early detection and diagnosis of prostate cancer.

• 동의생리병리학회지
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• 제19권5호
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• pp.1137-1141
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• 2005

This paper is written in order to investigate how Lee Je-Ma changed traditional Zhu Xi's philosophical view on Way(Tao; 道) Virtue(De: 德) Nature(Xing;性) and Imperative(Ming; 命), which was general concepts of his contemporary age, to practical philosophy and created new medical theories of Sasang(4 symbols) science from it. Lee emphasized the true thing which is able to be done in one's living not the concepts of Sasang. So he induced the concepts of Way and Virtue from the easy and simple way(易簡之道) in the Classic of Changes(I'Ching) for the purpose of practice by anyone. Because Nature and Imperative are derived from Way and Virtue in the context logically, human life is virtual in itself, so the substance of Nature and Imperative became equal with Way and Virtue. Herein the names and concepts of original 'Sasang' were substituted to Business(事), Mind(心), Body(身), Thing(物) which is able to be directly related with human being in his daily life. And he explained variously about the responses of Mind and Body of individuals onto Business and Thing. So it could be applied in medical aspects like emotional, symptomatical, characteristic features. Conclusively man is virtual being, so Nature and Imperative in the level of Human being are based on Way and Virtue in the level of Heaven. For that reason the new concepts of Sasang Business. Mind. Body Thing or Heaven. Human. Nature and Imperative were proposed.

• 동의신경정신과학회지
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• 제12궈1호
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• pp.3-10
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• 2001

Psychology and esters medicine are congenital therapeutic partners. they are closer in concept and practice to each other than either is to the principles of western science and medicine. Estern medicine, like most psychotherapies, is concerned with an individual's unique physical and emotional state. The aim of this study is to review Ii-Geug-Byun-Qi(移精變氣療法) therapy which is one of the psychotherapies in estern medicine. We studied few representative books which are about in estern medicine and also studied some clinical case reports on psychotherapies by estern medicine therapy, specially about Ii-Geug-Byun-Qi(移精變氣療法) therapy. Generally we understood Chukyoo(祝由) is the eldest psychological therapy which is based on Shamanism and controlled by Shaman as a doctor. and it is similar to simple psychoanalysis in western medicine. The psychologic therapy of Ii-Geug-Byun-Qi (移精變氣療法) is typical estem medicine psychologic therapy since the principle of estern medice had built like Hwang-Chi-Nei-Ching(黃帝內經). The meaning. of Ii-Geug-Byun-Qi(移精變氣療法) therapy is holistic psychologic therapy which are based on the harmony of mind and body. The practical method of Ii-Geug-Byun-Qi(移精變氣療法) are to refresh patient's emotional condition, like ventilaltion. The second healing method of Ii-Geug-Byun-Qi(移精變氣療法) is persuasion by dialogue. The third method is to clear patient's doubt by explainning of some qutestions. Therefore, the indication of Ii-Geug-Byun-Qi(移精變氣療法) therapy are psychosomatic disease or psychogenic problems. In conclusion, I suggest that the Ii-Geug-Byun-Qi(移精變氣療法) therapy could be powerfully adopt to prevent or treat lots of disease which is related with mind and body induced by stressor.

• Korean Circulation Journal
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• 제54권3호
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• pp.126-137
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• 2024

Background and Objectives: The impact of off-hours admission (such as weekends, nighttime, and non-working hours) vs. regular hours (weekdays and daytime working hours) on the mortality risk of patients undergoing surgery for type A aortic dissection (TAAD) repair is still uncertain. To address this uncertainty, we undertook a comprehensive systematic review and meta-analysis. We aimed to assess the potential link between off-hours admission and the risk of mortality in patients undergoing TAAD repair surgery. Methods: We conducted a thorough search of the PubMed, Embase, and Cochrane Library databases, covering the period from their inception to May 20, 2023. Our inclusion criteria encompassed all studies that examined the potential relationship between off-hour admission and mortality in individuals who had undergone surgery for TAAD repair. The odds ratios (ORs) were extracted and combined utilizing a random effects model for our synthesis. Results: Nine studies with 16,501 patients undergoing TAAD repair surgery were included in the meta-analysis. Overall, patients who underwent surgery during the weekend had higher in-hospital mortality (pooled OR, 1.41; 95% confidence interval [CI], 1.14-1.75; p=0.002) than those treated on weekdays. However, the mortality risks among patients who underwent TAAD surgery during nighttime and non-working hours were not significantly elevated compared to daytime and working hours admission. Conclusions: Weekend surgery for TAAD was associated with a higher in-hospital mortality risk than weekday surgery. However, further studies are warranted to identify and develop strategies to improve the quality of round-the-clock care for patients with TAAD.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4623-4626
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• 2012

Background: The risk of treatment-related death (TRD) and febrile neutropaenia (FN) with adjuvant taxane-based chemotherapy for early breast cancer is unknown in Malaysia despite its widespread usage in recent years. This study aims to determine these rates in patients treated in University Malaya Medical Centre (UMMC). Patients and Methods: Patients who were treated with adjuvant taxane-based chemotherapy for early breast cancer stages I, II or III from 2007-2011 in UMMC were identified from our UMMC Breast Cancer Registry. The TRD and FN rates were then determined retrospectively from medical records. TRD was defined as death occurring during or within 30 days of completing chemotherapy as a consequence of the chemotherapy treatment. FN was defined as an oral temperature > $38.5^{\circ}C$ or two consecutive readings of > $38.0^{\circ}C$ for 2 hours and an absolute neutrophil count < $0.5{\times}10^9/L$, or expected to fall below $0.5{\times}10^9/L$. Results: A total of 622 patients received adjuvant chemotherapy during this period. Of these patients 209 (33.6%) received taxane-based chemotherapy. 4 taxane-based regimens were used namely the FEC-D, TC, TAC and AC-PCX regimens. The commonest regimen employed was the FEC-D regimen accounting for 79.9% of the patients. The FN rate was 10% and there was no TRD. Conclusion: Adjuvant taxane-based chemotherapy in UMMC for early breast cancer has a FN rate of 10%. Primary prophylactic G-CSF should be considered for patients with any additional risk factor for FN.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8513-8517
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• 2016

Background: GLOBOCAN12 recently reported high cancer mortality in Malaysia suggesting its cancer health services are under-performing. Cancer survival is a key index of the overall effectiveness of health services in the management of patients. This report focuses on Subang Jaya Medical Centre (SJMC) care performance as measured by patient survival outcome for up to 5 years. Materials and Methods: All women with breast cancer treated at SJMC between 2008 and 2012 were enrolled for this observational cohort study. Mortality outcome was ascertained through record linkage with national death register, linkage with hospital registration system and finally through direct contact by phone or home visits. Results: A total of 675 patients treated between 2008 and 2012 were included in the present survival analysis, 65% with early breast cancer, 20% with locally advanced breast cancer (LABC) and 4% with metastatic breast cancer (MBC). The overall relative survival (RS) at 5 years was 88%. RS for stage I was 100% and for stage II, III and IV disease was 95%, 69% and 36% respectively. Conclusions: SJMC is among the first hospitals in Malaysia to embark on routine measurement of the performance of its cancer care services and its results are comparable to any leading centers in developed countries.