• 제목/요약/키워드: the C(S) extension

검색결과 558건 처리시간 0.028초

Isolation and Characterization of Oligotrophic Bacteria Possessing Induced Systemic Disease Resistance against Plant Pathogens

  • Han, Song-Hee;Kang, Beom-Ryong;Lee, Jang-Hoon;Kim, Hyun-Jung;Park, Ju-Yeon;Kim, Jeong-Jun;Kim, Young-Cheol
    • The Plant Pathology Journal
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    • 제28권1호
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    • pp.68-74
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    • 2012
  • Biocontrol microbes have mainly been screened among large collections of microorganisms $via.$ nutrient-rich $in$ $vitro$ assays to identify novel and effective isolates. However, thus far, isolates from only a few genera, mainly spore-forming bacilli, have been commercially developed. In order to isolate field-effective biocontrol microbes, we screened for more than 200 oligotrophic bacterial strains, isolated from rhizospheres of various soil samples in Korea, which induced systemic resistance against the soft-rot disease caused by $Pectobacterium$ $carotovorum$ SCC1; we subsequently conducted in $planta$ bioassay screening. Two oligotrophic bacterial strains were selected for induced systemic disease resistance against the $Tobacco$ $Mosaic$ $Virus$ and the gray mold disease caused by $Botrytis$ $cinerea$. The oligotrophic bacterial strains were identified as $Pseudomonas$ $manteilii$ B001 and $Bacillus$ $cereus$ C003 by biochemical analysis and the phylogenetic analysis of the 16S rRNA sequence. These bacterial strains did not exhibit any antifungal activities against plant pathogenic fungi but evidenced several other beneficial biocontrol traits, including phosphate solubilization and gelatin utilization. Collectively, our results indicate that the isolated oligotrophic bacterial strains possessing induced systemic disease resistance could provide useful tools as effective biopesticides and might be successfully used as cost-effective and preventive biocontrol agents in the field.

만성질환 보유정도에 따른 여름, 겨울 농촌 노인의 식이섭취 평가 (Dietary Intake Assessment by the Number of Chronic Diseases and the Season for Elderly Living in Rural Area)

  • 문현경;김정은;김은해
    • Journal of Nutrition and Health
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    • 제42권3호
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    • pp.221-233
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    • 2009
  • Korea's aging population has been remarkably increased. They want to have not only extension of life expectancy but also improving quality of life. To maintain the quality of life, it is essential to have good nutrition. However, nutritional status of elderly in Korea has problems qualitatively and quantitatively. Risk factors for poor nutrition are advanced age, woman, living alone and low economic status. Another risk factor in rural area is season because seasonal changes can affect food intake of elderly. Thus this study surveyed the health status and dietary intakes of elderly by season in rural area. In this study, the elderly were grouped as group 1 {elderly who have one risk factor for chronic diseases (obesity, hypertension, dyslipidemia, diabetes)} and group 2 (elderly who have more than 2 risk factors). Can-Pro 3.0 was used for dietary data analysis and SPSS 12.0 program was used for statistical analysis. Obesity had the highest percentage 62%, followed by hypertension 59.5%, dyslipidemia 21.5% and diabetes 11.6%. Obesity, hypertension, and dyslipidemia were high in winter and WHR, diabetes and anemia were high in summer. Mean intakes of energy and nutrients were less than RI. Nutrients which were changed most by season were vitamin A and Vitamin C. Intakes of calcium and folic acid were less than recommended in summer. The ratio of CPF for carbohydrate was higher and fat was lower than recommended. In conclusion, the nutrient intake of Group 2 was better in quality but Group 1 was better in quantity. Nutrient intakes were poor in summer. In rural area, more careful nutritional assesment and management are needed for aged population, especially in summer.

Botrytis cinerea에 의한 나도풍란 잿빛곰팡이병 발생 (Occurrence of Gray Mold on Aerides japonicum Caused by Botrytis cinerea in Korea)

  • 권진혁;지형진;윤재길
    • 식물병연구
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    • 제12권3호
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    • pp.287-289
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    • 2006
  • 2004년부터 2006년까지 3년 동안 경남 고성군과 통영시 나도풍란 재배 농가에서 Botrytis cinerea에 의한 잿빛곰팡이병이 발생하였다. 병징은 잎자루 부분이 수침상으로 물러지면서 부패되며 심하게 감염될 경우 시들어 말라 죽고 병반부위에 회색의 곰팡이가 많이 형성된다. 분생포자는 무색으로 단포자이며 난형 또는 타원형으로 크기는 $6{\sim}21{\times}4{\sim}13{\mu}m$였고, 분생포자경 위에 분생포자가 많이 형성되었다. 분생포자경은 갈색으로 격막이 있고, 폭은 $15{\sim}34{\mu}m$였다. 병의 원인 규명은 Koch의 가설에 따라 수행하였다. 병반에서 분리한 병원균의 균학적 특징을 조사한 결과 Botrytis cinerea Persoon: Fries로 동정 되었으며 본 병해는 국내에 보고된 바 없는 나도풍란 잿빛곰팡이병으로 진단되었다.

야생 효모 종류에 따른 알코올 발효 특성 (Characterization of Ethanol Fermentation with Wild Type Yeast Strains)

  • 백성열;이유정;김명동;이재형;문지영;여수환
    • 한국미생물·생명공학회지
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    • 제43권3호
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    • pp.227-235
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    • 2015
  • 발효식품에서 분리한 야생 효모 12 균주의 주류 제조 가능성을 조사하기 위해, 알코올 발효에 관여하는 특성을 분석하였다. 알코올 및 당 내성을 조사한 후, 당화액을 제조하여 pH, 고형분 함량, 적정산도, 아미노산도, 알코올, 유기산 및 향기성분 등을 분석하였다. S. cerevisiae 효모의 알코올 내성은 10%에서 보였으나 non-Saccharomyces 효모는 P. kudriavzevii N77-4를 제외하고 내성이 낮았다. 당 내성은 H. opuntiae HP1-2, C. tropicalis Y447을 제외한 효모에서 모두 우수하였다. 야생 효모를 이용한 알코올 발효액의 pH는 3.01−3.61, 고형분 함량은 8.73−13.10°Brix로 S. cerevisiae보다 non-Saccharomyces가 높았다. 적정산도는 0.25−0.33%로, 아미노산도는 1.61−2.61 g/100 ml으로 non-Saccharomyces 효모가 높은 값을 나타내었다. 알코올 함량은 2.25−8.5%로 S. cerevisiae에 비해 non-Saccharomyces 효모가 매우 낮았다. 야생 효모로 알코올 발효시 유기산은 사과산, 초산, 호박산 등이 증가하였고, 그 중 P. kudriavzevii N77-4는 낮은 초산 함량과 높은 사과산 및 호박산 함량을 나타내었다. 당화액을 전자코로 분석한 결과, DF1의 오른쪽 범주에 속했으며, 효모 발효액은 DF1의 왼쪽 범주에 속하여 발효에 따른 뚜렷한 차이를 보였다. 휘발성 향기성분은 non-Saccharomyces 효모에서 ethyl acetate가, S. cerevisiae 효모는 ethanol 화합물이 높은 향기성분을 나타내었다.

이류체 포그 시스템을 이용한 친환경적 가루이 방제시 올레산의 적정 농도 (Proper Application Concentration of Oleic Acid for Eco-friendly Control of Whiteflies by Two-fluid Fogging System in Greenhouses)

  • 김성은;이상돈;이문행;심상연;김영식
    • 생물환경조절학회지
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    • 제21권3호
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    • pp.299-304
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    • 2012
  • 본 연구는 친환경적이며 효과적인 시설내 가루이 예방 및 방제를 위해 이류체 포그시스템을 이용하여 올레산을 분무할 때, 올레산의 적정농도를 구명하고 3회 처리시 방제가를 조사하기 위해 수행되었다. 올레산의 적정농도 실험은 부여토마토시험장 단동형 플라스틱온실에서 공시품종으로 도태랑골드를 사용하여 수행했고, 올레산의 3회 처리시 방제가에 대한 실험은 경기도농업기술원 벤로형 유리온실에서 공시품종으로 로쿠산마루를 사용하여 수행했다. 적정농도 실험은 무처리구를 대조구로 하고 올레산 2000ppm과 4000ppm을 각각 처리하고 가루이의 밀도를 조사했다. 3회 처리 실험은 화학살충제 처리와 동일하게 올레산 2000ppm을 2일 간격으로 3회 처리하고, 2일 후에 방제가를 조사했다. 이류체 포그시스템은 실험기간동안 올레산을 처리하지 않을 때에는 오전 8시부터 오후 5시까지 온도가 $25^{\circ}C$ 이상이거나 습도가 75% 이하로 내려갈 때 180초 작동 후 30초 휴식을 반복하여 작동했고, 올레산을 처리했을 때에는 오전 11시부터 오후 5시까지 동일한 조건으로 작동했다. 올레산의 농도실험에서는 2000ppm에서 81.6%, 4000ppm에서 93.6%의 방제가를 나타냈다. 즉, 농도를 높게 처리할수록 가루이의 밀도는 크게 감소했다. 올레산 3회 처리 실험에서는 2000ppm을 3회 처리했을 때, 농약등록 기준보다 높은 85.8%의 방제가를 나타냈다. 따라서 올레산을 2000ppm 이상 3회 처리하는 것이 친환경적인 가루이의 예방 및 방제에 매우 효과적인 것으로 나타났다.

불량환경하(不良環境下)에서의 제초제(除草劑) 약해(藥害)와 경감기술(輕減技術) (Herbicidal Phytotoxicity under Adverse Environments and Countermeasures)

  • 권용웅;황형식;강병화
    • 한국잡초학회지
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    • 제13권4호
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    • pp.210-233
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    • 1993
  • The herbicide has become indispensable as much as nitrogen fertilizer in Korean agriculture from 1970 onwards. It is estimated that in 1991 more than 40 herbicides were registered for rice crop and treated to an area 1.41 times the rice acreage ; more than 30 herbicides were registered for field crops and treated to 89% of the crop area ; the treatment acreage of 3 non-selective foliar-applied herbicides reached 2,555 thousand hectares. During the last 25 years herbicides have benefited the Korean farmers substantially in labor, cost and time of farming. Any herbicide which causes crop injury in ordinary uses is not allowed to register in most country. Herbicides, however, can cause crop injury more or less when they are misused, abused or used under adverse environments. The herbicide use more than 100% of crop acreage means an increased probability of which herbicides are used wrong or under adverse situation. This is true as evidenced by that about 25% of farmers have experienced the herbicide caused crop injury more than once during last 10 years on authors' nationwide surveys in 1992 and 1993 ; one-half of the injury incidences were with crop yield loss greater than 10%. Crop injury caused by herbicide had not occurred to a serious extent in the 1960s when the herbicides fewer than 5 were used by farmers to the field less than 12% of total acreage. Farmers ascribed about 53% of the herbicidal injury incidences at their fields to their misuses such as overdose, careless or improper application, off-time application or wrong choice of the herbicide, etc. While 47% of the incidences were mainly due to adverse natural conditions. Such misuses can be reduced to a minimum through enhanced education/extension services for right uses and, although undesirable, increased farmers' experiences of phytotoxicity. The most difficult primary problem arises from lack of countermeasures for farmers to cope with various adverse environmental conditions. At present almost all the herbicides have"Do not use!" instructions on label to avoid crop injury under adverse environments. These "Do not use!" situations Include sandy, highly percolating, or infertile soils, cool water gushing paddy, poorly draining paddy, terraced paddy, too wet or dry soils, days of abnormally cool or high air temperature, etc. Meanwhile, the cultivated lands are under poor conditions : the average organic matter content ranges 2.5 to 2.8% in paddy soil and 2.0 to 2.6% in upland soil ; the canon exchange capacity ranges 8 to 12 m.e. ; approximately 43% of paddy and 56% of upland are of sandy to sandy gravel soil ; only 42% of paddy and 16% of upland fields are on flat land. The present situation would mean that about 40 to 50% of soil applied herbicides are used on the field where the label instructs "Do not use!". Yet no positive effort has been made for 25 years long by government or companies to develop countermeasures. It is a really sophisticated social problem. In the 1960s and 1970s a subside program to incoporate hillside red clayish soil into sandy paddy as well as campaign for increased application of compost to the field had been operating. Yet majority of the sandy soils remains sandy and the program and campaign had been stopped. With regard to this sandy soil problem the authors have developed a method of "split application of a herbicide onto sandy soil field". A model case study has been carried out with success and is introduced with key procedure in this paper. Climate is variable in its nature. Among the climatic components sudden fall or rise in temperature is hardly avoidable for a crop plant. Our spring air temperature fluctuates so much ; for example, the daily mean air temperature of Inchon city varied from 6.31 to $16.81^{\circ}C$ on April 20, early seeding time of crops, within${\times}$2Sd range of 30 year records. Seeding early in season means an increased liability to phytotoxicity, and this will be more evident in direct water-seeding of rice. About 20% of farmers depend on the cold underground-water pumped for rice irrigation. If the well is deep over 70m, the fresh water may be about $10^{\circ}C$ cold. The water should be warmed to about $20^{\circ}C$ before irrigation. This is not so practiced well by farmers. In addition to the forementioned adverse conditions there exist many other aspects to be amended. Among them the worst for liquid spray type herbicides is almost total lacking in proper knowledge of nozzle types and concern with even spray by the administrative, rural extension officers, company and farmers. Even not available in the market are the nozzles and sprayers appropriate for herbicides spray. Most people perceive all the pesticide sprayers same and concern much with the speed and easiness of spray, not with correct spray. There exist many points to be improved to minimize herbicidal phytotoxicity in Korea and many ways to achieve the goal. First of all it is suggested that 1) the present evaluation of a new herbicide at standard and double doses in registration trials is to be an evaluation for standard, double and triple doses to exploit the response slope in making decision for approval and recommendation of different dose for different situation on label, 2) the government is to recognize the facts and nature of the present problem to correct the present misperceptions and to develop an appropriate national program for improvement of soil conditions, spray equipment, extention manpower and services, 3) the researchers are to enhance researches on the countermeasures and 4) the herbicide makers/dealers are to correct their misperceptions and policy for sales, to develop database on the detailed use conditions of consumer one by one and to serve the consumers with direct counsel based on the database.

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Effects of Moisture and Barrel Temperature of Extrusion Process on Physicochemical and Functional Properties of Specialty Rice Cultivars

  • Choi, In-Duck;Song, Jin;Lee, Choon-Ki;Kim, Kee-Jong;Suh, Sea-Jung;Son, Jong-Rok;Ryu, Gi-Hyung;Kim, Jae-Hyun
    • Food Science and Biotechnology
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    • 제17권2호
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    • pp.319-323
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    • 2008
  • Mutant rice cv. Goami2 (G2) and Baegjinjoo (BJJ) derived from a high-quality japonica rice cv. Ilpumbyeo (IP) were extruded under different feed moisture (20 and 30%) and barrel temperature (90, 110, and $130^{\circ}C$). Increasing feed moisture at fixed barrel temperature increased extrudate density (ED) in IP and BJJ. Whereas, G2 showed a varied ED depending on extrusion conditions; increasing barrel temperature decreased the ED of G2 extrudate with low feed moisture, but increased with high moisture. Results indicated a positive barrcl temperature effect on volume expansion in IP and G2, but a negative effect on 811, probably due to shrinkage of expanded products containing low-amylose contents. A significant increase of water absorption was found in G2 and BJJ extruded flour, while an increase of water solubility in those from IP. Non-digestible carbohydrates measured by total dietary fiber (TDF) indicated that extrusion increased slightly TDF in IP and BJJ extrudates, but decreased in G2 products, which might be variety-dependent.

삼국시대(三國時代) 병기체제(兵器體制)의 연구(硏究) (This Study of the Arms Used in the Three Kingdoms)

  • 김성태
    • 헤리티지:역사와 과학
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    • 제34권
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    • pp.20-58
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    • 2001
  • In order to unravel the characteristics of arms used in the 'Three Kingdoms,' Kokuryo, Silla and Paikje. the classification and the developing procedures of the arms should be first discussed. At first, the basic arms of the soldiers of Three Kingdoms were iron swords, iron spearheads, and bows. During that period, swords attached a ring pommel were commonly used. But after 5A. D. a sword with a decoration pommel appeared. Infantry generally used iron spearheads. From the late 4A. D. the long spearheads were broadly used in cavalry battles. In the late 6A. D. infantry mainly used long spearheads, and this resulted in the foundation of long spearheads units. There were two kinds of bows: Short Bow whose arch is small and Long Bow whose arch is long. It is known that the Short Bow was widely used in Kokuryo and Paikje up to 5A. D. In the early era, infantry used Long Bow, yet it was vastly used after 6A. D. when a castle's strategical value was great and defending a castle was. significant. Above mentioned, as basic combat weapons, iron spearhead and bow were fundamental. In particular, the spearhead was the essential weapon to a soldier. Yet, arrow gun and hook-shape cutters were important weapons. Especially, after 6A.D., when a castle became strategically pivotal in military, the arrow gun became the important weapon. This resulted in the foundation of arrow gun units. Hook-shape cutters were used to snatch horsemen or to climb up to fall the castle. Yet, the cutter was not the Three Kingodoms' basic weapon. In addition, the three stages of arms development in the Three Kingdoms are formation stage, development stage, and settlement stage. The formation stage was the period when premitive military unit appeared in the Three Kingdoms. It ranged from 1B. C. to the mid 3A. D. At that time according to regions. there were two weapon systems operating: North area including Kokuryo and the northern part of Paikje and South area including Silla, Kaya and the southern part of Paikje. ln North area a sword with a ring attached at the end of the holder, iron spear with neck and mid-size flat holder and iron arrowhead with an extension to fix, were used. In this period, during a war calvary units were mostly used and their weapon systems seemed possibly to succeed in that of Kochosun. In the development stage, when LoLang's influence on surroundings became weak, Koguryo, Paikjae and Silla had directly contacted each other. In the late 3A.D. to the early 6A.D., Silla achieved a drastic improvement in weapon system. This was the period when Kokuryo played a leading role in arms race. Kokuryo's arms manufacturing techniques passed onto Silla, Kaya and Paikje. In combat strategy a joint operation between infantry and calvary prevailed even if their military tactics were different. In a calvary battle heavily armed horsemen played import roles at this period. The horsemen and even horses were heavily guarded with iron armors. After all, the appearance of fully armed horsemen implies the very need of powerful destructive forces in weapon system. At that time, basic weapons were a big sword with a ring attached at the end of the holder, swallow's tail-shape spear with neck, and iron spearhead with neck and an extension. The settlement stage began at the mid 6A.D., when it was the revolutionary period in the arms development history. Of course, actual proofs and picture documents were not sufficient enough to penetrate full scale of the weapon system. But, according to historical circumstances and historic records, it is very certain that this period was the peak in arms development. In this period special military units, such as infantry-calvary companies, Archery units and Long spear units, that executed particular duties with special weapons, were founded. This became the characteristics of the settlement stage.

고추와 재배환경의 식품매개 병원균 분포 (Distribution of foodborne pathogens in red pepper and environment)

  • 정지은;서승미;양수인;진현숙;정규석;노은정;정명인;류재기;류경열;오광교
    • 한국식품과학회지
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    • 제53권6호
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    • pp.799-808
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    • 2021
  • 본 연구는 고추와 토양, 농업용수, 장갑을 대상으로 101개의 시료를 채취하여 위생지표세균(일반세균, 대장균군, 대장균)과 B. cereus, S. aureus에 대해 정량 분석과 병원성 세균(병원성 E. coli, E. coli O157:H7, B. cereus, S. aureus, L. monocytogenes, Salmonella spp.)에 대해 정성 분석하였다. 고추와 토양, 장갑 시료의 일반세균수는 3.36-7.08 log CFU/g, 대장균군은 2.16-5.14 log CFU/g, 대장균은 1.50-1.54 log CFU/g이었고 농업용수 시료의 대장균군은 17.67-247.05 MPN/100 mL 수준으로 나타났다. 고추와 토양, 농업용수, 장갑의 B. cereus는 1.35-6.78 log CFU/g, S. aureus는 1.63-2.09 log CFU/g 수준으로 나타났다. 고추와 토양, 농업용수, 장갑 시료에서 병원성 E. coli, E. coli O157:H7, L. monocytogenes, Salmonella spp. 등은 검출되지 않았다. 고추와 환경 시료에서 분리한 B. cereus의 장독소 유전자와 항생제 감수성을 분석하였다. 고추와 토양, 장갑에서 분리한 B. cereus의 hblACD 유전자는 각각 54.5, 75, 75% 비율이었으며, nheABC유전자는 각각 72.7, 75, 100% 비율이었다. 고추와 토양, 장갑에서 분리한 B. cereus는 β-lactam계 항생제에 대해 저항을 보였지만, cefotaxime에 대해 일부 균주는 중간 내성을 보였고 모든 균주는 imipenem에 대해 감수성을 나타냈다. B. cereus는 비β-lactam계 항생제에 대해 감수성을 나타냈지만, rifampin, trimethoprim-sulfamethoxaole, vancomycin, clindamycin, erythromycin에 대해 일부 균주는 중간 내성을 나타냈다. 본 연구의 결과를 통하여 재배단계 고추의 미생물학적 오염도를 파악할 수 있고, 고추에 오염된 B. cereus에 의해 설사형 식중독이 발생 가능성을 파악할 수 있다. 이는 농산물 중 미생물 기준 설정 등에 대한 과학적 근거로써 활용이 가능할 것으로 기대한다. 또한, 고추와 재배환경에서 항생제 저항성 B. cereus가 검출되어 농업현장에서 항생제 내성균주 출현을 예방하는 대책이 요구된다.

Bone Mineral Density and Affecting Factors in College Women

  • Na, Hye-Bok;Jung, Shin-Yong
    • Journal of Community Nutrition
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    • 제1권2호
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    • pp.98-107
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    • 1999
  • The purpose of this study was to investigate Bone Mineral Density(BMD) and affecting factors on BMD of college women in Seoul. The subjects were 47 healthy college women aged 18-25 years. Antrophometric and body fat measurements were performed by Bioelectrical Impedance Fatness Analyzer(Tanita TVF 202). Blood pressure and pulse frequency were measured. Dietary intakes and general living habits were examined through questionnaires and nutrient intakes were analyzed by Computer Aided Nutritional Analysis(CAN) program for professional. Serum total cholesterol, TG(triglyceride), HDL-cholesterol, total protein, albumin, GOT, calcium were measured by Spotchem(SP-4410). Serum osteocalcin and alkaline phosphatase(ALP) were measured to monitor bone formation. BMD of lumbar spine(L2-L4), right hip(neck, ward's triangle, trochanter) and right forearm were measured by Dual Energy X-ray Absorptiometry(DEXA). Muscle strength was measured by examining leg flexion strength(right and left), leg extension power(right and left), handgrip power(right and left) and back strength. All data were statistically analyzed by the SAS PC package program. BMD of college women was normal(by WHO, 1994). Their muscle strength was bad(by national fitness guidebook, 1995). Only a mall number of them exercised (32.6% of subjects). There was no significant difference among BMD, muscle strength and % body fat(p<0.05). There were significant differences between BMD and total cholesterol as well as TG and VLDL-cholesterol(p<0.05). Total cholesterol was associated with decreasing BMD of the right forearm(p<0.05). TG and VLDL-cholesterol are associated with increasing BMD of right hip ward's triangle(p<0.05). There were significant differences among BMD, ALP and serum total protein. ALP was associated with decreasing BMD of the right forearm(p<0.05). There were significant differences between BMD and Ca as well as between Na and K intakes (p<0.05). Intakes of Na and K were associated with decreasing BMD of the right forearm (UD)(p<0.05). There were significant differences between in BMD and pulse frequently and serum albumin (p<0.05). Serum albumin is associated with increasing BMD of L3-L4(p<0.05), right hip neck(p<0.05). %Body fat, TG, VLDL-C, Ca intake, pulse frequency and serum albumin were associated with increasing BMD(p<0.05). Intakes of Na and K, ALP, total cholesterol, total cholesterol, total protein and height are associated with decreasing BMD(p<0.05). Overall results indicate that Ca intake but to be moderate in protein and Na intakes in order to increase BMD. Body exercise was recommended to increase BMD as well.

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