• 한국생물환경조절학회:학술대회논문집
• /
• 한국생물환경조절학회 2007년도 춘계 학술대회 및 정기총회
• /
• pp.271-275
• /
• 2007

• 한국생물환경조절학회:학술대회논문집
• /
• 한국생물환경조절학회 2007년도 춘계 학술대회 및 정기총회
• /
• pp.276-281
• /
• 2007

• 한국생물환경조절학회:학술대회논문집
• /
• 한국생물환경조절학회 2007년도 춘계 학술대회 및 정기총회
• /
• pp.266-270
• /
• 2007

• 대한지역사회영양학회지
• /
• 제16권1호
• /
• pp.75-85
• /
• 2011

The purpose of this study was to investigate basic data on the degree of parents' recognition and satisfaction with the use of environment-friendly agricultural products. The conclusions of this study are as follows. The survey on the use of environment-friendly products in households showed that 68.7% of the respondents used environment-friendly products at home. The frequency of purchasing organic products by household consumers showed that 25.1% of the respondents bought organic products one to two times per week. The most popular items of the environment-friendly agricultural products used were vegetables, comprising 63.9%. The households with more than 3,000,000 won of monthly income tended to consume more domestic livestock products (p < 0.05). In comparison to general agricultural products, environment-friendly agricultural products users showed higher satisfaction level with a better quality in term of safety, quality, nutrition, taste, appearance, diversity and price. The degree of satisfaction with safety was the highest ($3.8{\pm}0.68$), and price showed the lowest ($2.76{\pm}0.79$). The degree of parents' recognition of the governmental support for environment-friendly agricultural products showed that those who were aware of the supporting policy were 44.5%, but 55.5% of them didn't know about the fact that 46.6% of the respondents agreed that environment-friendly agricultural products should be used in more than 50% of the foods used for school food service. The respondents pointed out that vegetables (45%) sprayed with pesticide are the most urgent food item that need to be replaced by environment-friendly farm products.

• Mycobiology
• /
• 제38권1호
• /
• pp.74-77
• /
• 2010

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from $15\sim35^{\circ}C$. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at $25^{\circ}C$ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.

• 한국농림기상학회:학술대회논문집
• /
• 한국농림기상학회 2005년도 추계 학술발표논문집
• /
• pp.88-92
• /
• 2005

• International Journal of Industrial Entomology and Biomaterials
• /
• 제18권2호
• /
• pp.125-129
• /
• 2009

Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.

• 한국유기농업학회지
• /
• 제28권4호
• /
• pp.555-577
• /
• 2020

This study analyzes the management performance and productivity of environmentfriendly farms compared to conventional farms and the trend of changes in price premium rates of environment-friendly agricultural products. And environmentfriendly farms in Jeollanam-do are surveyed for difficulties in management, proper premium rate of environment-friendly agricultural products (WTA), and tasks for promoting sales. According to the analysis results, the management performance and productivity of are low in many items, and the number of items that are on the decline or stagnant in the environment-friendly premium is making it difficult for farmers to manage. According to a farm survey, the most important task is to promote school meals for boosting sales of environment-friendly agricultural products. And 65.5% of the respondents having contract cultivation, nearly half or 41.1% of the respondents said they do not need contract cultivation or want contract cultivation for less than one year, which means that the current contract does not meet the needs of farmers. Finally, the environment-friendly premium rate based on consumer prices is generally lower than the premium rate (WTA) that farmers perceive as appropriate, so it is important to resolve the gap between the actual premium rate and the WTA.

• 한국응용곤충학회지
• /
• 제56권4호
• /
• pp.403-412
• /
• 2017

춘천지역 농가 및 강원도농업기술원 내 시험포장에서 반촉성재배토마토(2~6월)에서 트랩을 이용한 주요 해충의 발생밀도와 천적 등을 이용한 방제효과를 조사 하였다. 토마토 재배농가에서 주로 사용하는 약제와 유기농자재의 천적곤충에 대한 독성평가도 수행하였다. 반촉성재배 시 발생되는 주요 해충은 온실가루이(Trialeurodes vaporariorum)와 총채벌레류이었다. 온실가루이의 경우 4월 중순경부터 발생하기 시작하여 5월 중순 이후 발생이 증가하였고, 총채벌레류는 4월 상순 경 발생을 시작하여 6월 이후에 증가하는 경향을 보였다. 저독성약제 선발시험을 통해 온실가루이와 총채벌레의 천적인 온실가루이좀벌과 미끌애꽃노린재에 대해 60% 이하의 독성을 보이는 살충제 1종(spiromesifen SC), 살균제 1종(cyazofamid SC) 및 님 추출물이 함유된 유기농자재 외 1종을 선발하였다. 이상의 결과에서 제시한 천적중심 관리모델을 적극적으로 활용한다면 토마토 반촉성 시설재배 시 온실가루이와 총채벌레를 효율적으로 관리할 수 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
• /
• 제20권1호
• /
• pp.45-53
• /
• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.