• Journal of Embryo Transfer
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• v.23 no.1
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• pp.1-4
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• 2008

This study was performed to investigate the effect of laser-assisted hole in the zona pellucida (ZP) of frozen-thawed ICR mouse embryos on the process of hatching that is critical for expanded blastocysts to implant into endometrium, Vitrification medium, composed of ethylene glycol and sucrose supplemented with 7.5% (w/v) PVP, was used to freeze $2{\sim}4$ cell stage embryos recovered from oviducts of superovulated and mated female mice before storing them in $LN_2$. Right after thawing them, a laser beam was shot to make a hole in ZP followed by culturing in KSOM for $96{\sim}120\;hr$ and examining development to blastocyst and hatching every 12 hr. Laser-treated embryos showed significantly higher hatching rate compared to control (92.9% vs. 22.1%, p<0.05). From around Day 4, blastocysts developed from laser-treated embryos started hatching while the blastocysts of control group failed to hatch showing a lot of shrinkage. This study shows that a laser-assisted hole in ZP improves the hatching rate of blastocysts developed from frozen-thawed, in vitro cultured ICR mouse embryos.

• Journal of Pharmaceutical Investigation
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• v.37 no.1
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• pp.51-55
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• 2007

Solid lipid nanoparticles (SLNs) have been regarded to behave similar to the vegetable oil emulsions because emulsions of lipid melts are formed before lipid droplets being solidified to turn into SLNs. Compared to lipid emulsion, however, it has been more difficult to obtain stable SLNs and needs more extensive considerations on stabilizer and manufacturing process. In the present study, we tried to prepare phosphatidylcholine-based trymyristin (TM) SLNs using high pressure homogenization method and optimize the manufacturing variables such as homogenization pressure, number of homogenization cycles, cooling temperature, co-stabilizer and freeze-drying with cryoprotectants. Nano-sized TM particles could be Prepared using egg Phosphatidylcholine and pegylated phospholipids ($PEG_{2000}$PE) as stabilizers. Based on the optimization study, the dispersion was manufactured by homogenization under the pressure of 100 MPa for more than 5 cycles, and solidifying the intermediately formed lipid melt droplets by dipping in liquid nitrogen followed by thawing at room temperature. In addition, TM SLNs could be freeze-dried and then redispersed easily without significant particle size changes after freeze drying with 10% and 12.5% sucrose or trehalose. The TM SLNs established in this study can be used as delivery system for drugs and cosmetics.

• Microbiology and Biotechnology Letters
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• v.20 no.3
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• pp.243-248
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• 1992

In order to examine the viability depending on rehydration process and membrane injury of Nocardia mediterranei upon lyophilization, We labeled $3^H$-thymidine in deoxyribonucleic acid of N. mediterrranei to obtain information on the mechanisms of injury caused by lyophilization. Suspensions of rehydrated cells were incubated with added DNase in a buffer solution. Extracellular radioactivity levels appeared to be high in the rehydrated solutions after lyophilization than freezing-thawing. Thus, the membrane systems were injured by lyophilization, but not ovenvhelmed. These considerations were confirmed by electron microscopy. In effects of rehydration, the cell membrane was seriously damaged by strong atmospheric pressure as soon as the inner ampule was opened, but this was not the case without admitting air under vacuum. N. rnediterranei cells, with no additives, were lyophilized and reconstituted without admitting air, virtually about 84% of the cells were viable.

• Journal of the Korean Ceramic Society
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• v.55 no.1
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• pp.36-43
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• 2018

The physical and chemical analyses of mineral waste such as moisture content, water absorption, freezing-thawing resisting sexual, chemical composition and crystal structure were investigated. In the technological process of crushing, screening, molding, drying, preheating, sintering and cooling, many parameters were changed to eliminate the influence of freeze thaw stability and the ball billets were processed into slate ceramsites eventually. Adopting orthogonal experiment and range analysis, the optimal technology parameters were confirmed as preheating temperature of $300^{\circ}C$ for 25 minutes and sintering temperature of $1230^{\circ}C$ for 20 minutes. Slate wastes in Shangri-la could foam and expand without any additive. The ultra-light ceramsite could be directly used as building aggregate, since the analysis results of its leaching toxicity were eligible. Besides, effects of sintering temperature on physical property and crystal phase were also explored in this study.

• Applied Biological Chemistry
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• v.38 no.6
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• pp.512-515
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• 1995

In order to examine the effect of rehydration methods on viability after freeze-drying of Streptomyces aureofaciens, we labeled the DNA of S. aureofaciens with $^3H-adenine$. Extracellular radioactivity levels appeared to be high in the rehydrated solutions after freeze-drying than freezing-thawing. In effects of rehydration after freeze-drying, the viability of the cell appeared about 20% in case of with admitting air at single ampule, but that of which appeared about 91% in case of without admitting air at double ampule. Thus, S. aureofaciens cells were damaged during the process of rehydration after freeze-drying.

• Korean Journal of Environmental Agriculture
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• v.41 no.3
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• pp.217-221
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• 2022

BACKGROUND: Gotjawal forest on Jeju Island is characterized by uneven topography that developed as a result of freezing-thawing weathering process and irregular substrates caused by numerous lava-flow collapses. Sanyang Gotjawal, located in southwest of Jeju Island, is a well-developed forest with a long history. In addition to photographs, there is a need for a good way to describe the unique features of Gotjawal, including its geology and vegetation. METHODS AND RESULTS: We illustrated the area's natural features using Clip Studio Paint 1.12.0. To reveal its complexity, we separated the vegetation and geological features in the illustrations. CONCLUSION(S): We drew a cross-section of Gotjawal's unique layers, including lava flows. In addition to an eco-geological approach, we described the dominant vegetation and geological characteristics of the three forest layers (trees, shrubs, and herbs) in Gotjawal.

• Smart Media Journal
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• v.12 no.3
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• pp.77-84
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• 2023

Forest fires are representative natural disasters resulting from dramatic global climate change in these modern times. When forest formation is insufficient due to forest damage caused by fire, secondary damages such as landslides occur during the winter thawing period and heavy rains. In most countries, only a limited area is managed as CCTV-centered monitoring systems for forest management. For the landslide prediction, markers containing 3D spatial coordinates were located on the slopes of the danger areas in advance. Then 3D mapping and angle of repose were obtained by periodic drone imaging. The recognition range and angle of view of markers were defined, and a new method for predicting signs of landslides in advance was presented in this study.

• The Journal of Engineering Geology
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• v.24 no.1
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• pp.111-122
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• 2014

For accurate laboratory evaluations of soil deposits, it is essential that the samples are undisturbed. An artificial ground-freezing system is the one of the most effective methods for obtaining undisturbed samples from sand deposits. The objective of this study is to estimate the shear strengths and the characteristics of elastic waves of frozen-thawed and unfrozen specimens through the undrained triaxial compression test. For the experiments, Jumunjin standard sands are used to prepare frozen and unfrozen specimens with similar relative densities (60% and 80%). The water pluviation method is used to simulate the fully saturated condition under the groundwater table. When thawing the frozen specimens, the temperature is measured every minute. After the specimens are completely thawed, undrained triaxial compression tests are conducted using the same procedures as for the unfrozen specimens. During the triaxial tests (saturation, consolidation, and shear phase), compressional and shear waves are measured. The results show that the freeze-thaw process has minor effects on the peak deviatoric stress and shear strength values, and that the process does not affect the internal friction angle. The compressional wave velocity increases with increasing B-value to 1800 m/s in the saturation phase, but tends to remain constant in the process of consolidation and shearing. The shear wave velocity decreases with increasing B-value in the process of saturation, but changes velocity in accordance with the change in effective stress in the processes of consolidation and shearing. The compressional wave velocity has similar values regardless of the freeze-thaw process, but values of shear wave velocity are slighly lower in frozen-thawed specimens than in unfrozen specimens. This study is a preliminary experiment for estimating the shear strength and characteristics of elastic wave velocity in undisturbed frozen specimens that have been obtained using the artificial ground-freezing method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.8
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• pp.4033-4038
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• 2013

Sedimentary rocks from construction waste are discarded through open storage and landfilling, which causes an increase in construction cost and inefficient of execution of works. Some sandstone are selected and utilized as aggregates, but shale is buried as industrial waste. Therefore, in this research, we evaluated weathering properties of shale aggregate that is widely distributed throughout Daegu-Kyeongbuk region and freeze-thaw characteristics of concrete according to the replacement ratio of shale aggregate, in an effort to stabilize aggregate supply-demand in Daegu-Kyeongbuk region and develop alternative aggregates. We used red shale and black shale in the experiment, which were exported from a construction site in Deagu. We verified the usage of shale as a concrete aggregate by comparing andesite, which is broadly used as a thick aggregate for concrete, to hornfels, which is a metamorphic sedimentary rock. As a result of the experiment, we observed no degradation phenomenon for andesite and hornfels. However, a part of country rock containing black shale was found to be exfoliated. Red shale started having cracks in the direction of stratification after 1.5 months of direct exposure, and it broke into smaller pieces after approximately 4 months. After 300 cycles of freeze-thaw process on the concrete manufactured according to the replacement ratio of shale aggregate, the modulus of elasticity was 97% for plain and 95% for hornfels. In the case of RS_100, it was 57% after 210 cycles, and for BS_100, it was 54% after 240 cycles. Therefore, we established that, as the number of repetition increases, the freeze-thaw resistance decreases dramatically.

• Journal of the Korean GEO-environmental Society
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• v.21 no.9
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• pp.25-32
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• 2020

The underground utilities installed under the ground is an important civil engineering structure, such as water supply and sewerage pipes, underground power lines, various communication lines, and city gas pipes. Such underground utilities can be exposed to risk due to external factors such as concentrated rainfall and vehicle load, and it is important to select and construct an appropriate backfill material. Currently, a method mainly used is to fill the soil around the underground utilities and compact it. But it is difficult to compact the lower part of the buried pipe and the compaction efficiency decreases, reducing the stability of the underground utilities and causing various damages. In addition, there are disadvantages such as a decrease in ground strength due to disturbance of the ground, a complicated construction process, and construction costs increase because the construction period becomes longer, and civil complaints due to traffic restrictions. One way to solve this problem is to use a liquid filler. The liquid filler has advantages such as self-leveling ability, self-compaction, fluidity, artificial strength control, and low strength that can be re-excavated for maintenance. In this study, uniaxial compression strength test and fluidity test were performed to characterize the mixed soil using marine clay, stabilizer, and in-situ soil as backfill material. A freezing-thawing test was performed to understand the strength characteristics of the liquid filler by freezing, and in order to examine the effect of the filling materials on the corrosion of the underground pipe, an electrical resistivity test and a pH test were performed.