• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.7-12
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• 2001

Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts. The effect of some factors on the post-thaw survival of a total of 240 in vitro produced bovine blastocysts was investigated using logistic regression analysis. The explanatory variables tested were: type of culture medium before freezing (TCM 199 supplemented with BSA, BSAITS (BSA+insulin+transferrin+selenium), ECS (estrous cow serum) with or without BOEC (bovine oviductal epithelial cells), age of the blastocyst (Day 7, Day 8+9), morphological appearance before freezing (distinct=Q1 or indistinct=Q2 inner cell mass) and type of cryoprotectant (glycerol, 1.0 M or ethylene glycol, 1.6 M). The survival after thawing based on the post-thaw quality and the development after co-culture with BOEC for 24 and 48 hours. Day 7 blastocysts had an almost three times better chance of survival than Day 8+9 blastocysts. Q1, Day 8+9 blastocysts had higher odds to survive after 48 hours in culture than Q2 blastocysts (p<0.05). Blastocysts produced in BSAITS medium had the best chances of survival; however, the odds were not always significant. Blastocysts frozen in glycerol had a better post-thaw quality rating than those frozen in ethylene glycol; however, the difference in post-thaw development at culture was not significant. The relationship between post-thaw quality and post-thaw development at culture was significant (p<0.05). The developmental stage and/or age of the embryo and culture medium where development up to blastocyst takes place affect the post-thaw survival of the bovine embryos.

• Geomechanics and Engineering
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• v.25 no.1
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• pp.41-48
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• 2021

In cold regions, the integrity of the infrastructures built on weak soils can be extensively damaged by weathering actions due to the cyclic freezing and thawing. This damage can be mitigated by exploiting soil stabilization techniques. Generally, ordinary Portland cement (OPC) is the most commonly used binding material for investigating the chemo-hydromechanical behavior. However, due to the environmental issue of OPC producing a significant amount of carbon dioxide emission, calcium sulfoaluminate (CSA) cement can be used as one of the eco-sustainable alternatives. Although recently several studies have examined the strength development of CSA treated sand, no research has been concerned about CSA cement-stabilized sand affected by cyclic freeze and thaw. This study aims to conduct a comprehensive laboratory work to assess the effect of the cyclic freeze-thaw action on strength and durability of CSA cement-treated sand. For this purpose, unconfined compressive strength (UCS) and ultrasonic pulse velocity (UPV) tests were performed on the stabilized soil specimens cured for 7 and 14 days which are subjected to 0, 1, 3, 5, and 7 freeze-thaw cycles. The test results show that the strength and durability index of the samples decrease with the increase of the freeze-thaw cycles. The loss of the strength and durability considerably decreases for all soil samples subjected to the freeze-thaw cycles. Overall, the use of CSA as a stabilizer for sandy soils would be an eco-friendly option to achieve sufficient strength and durability against the freeze-thaw action in cold regions.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.725-730
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• 2002

The durability of concrete involves resistance to freeze-thaw action, corrosion, permeation, carbonation, chemical attack and so on. Generally, properties of concrete have been well understood under the separate action of these deterioration mechanisms. However, in practice, the degradation of concrete usually is the result of combined action of physical and chemical attack and can be accelerated by the combined action of several deterioration mechanisms. In the present study, to evaluate the combined deterioration by freeze-thaw action and seawater attack, ground granulated blast-furnace slag or silica fume concrete with water or seawater as mixing water was exposed to 210 cycles of freeze-thaw action. Tests were conducted to determined the relative dynamic modulus of elasticity and compressive strength. Furthermore, The XRD, SEM and EDS analysis were performed on the deteriorated part of concrete due to freeze-thaw action and seawater attack.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.593-598
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• 2002

Recently, as industrialization is rapidly growing and the standard of life is rising, the quantities of waste glasses have been hastily increased and most of them are not recycled but abandoned. It causes some problems such as the waste of natural resources and environmental pollution. Therefore, in this study freeze-thaw resistance test was conducted to analyze the properties of concrete containing waste glasses as fine aggregates and containing industrial by-products (fly ashes, silica fumes). As a results, it was found that freeze-thaw resistance decreases as the content of waste glasses increases. Also, the content of fly ash doesn't affect to the freeze-thaw resistance, and freeze-thaw resistance decreases with tile increase of silica fume contents.

• Journal of the Korean Ceramic Society
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• v.28 no.11
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• pp.917-925
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• 1991

For the present experiment five Portland cement mortars are in order: mortars with two different water/ cement ratios (W/C=0.45 and 0.50, each having no chemical additive), and those with an additive such as superplasticizer, air-entraining agent or water-repelling agent. We fix the W/C ratio of mortars having additive so that their pastes can yield the same workability as that of the cement mortar of W/C=0.50 with no additive. It is shown that the freeze-thaw resistivity depends heavily on the characteristic of wide pores. Despite a good deal of wide pores, the air-entrained specimen shows a good freeze-thaw resistivity due to appropriate air-pores. And also the specimen with water-repelling agent, which proves to cause the microstructure to become hydrophobic, make good resistance to freeze-thaw cycles in spite of its high wide-porosity. Our suggestion is that the freeze-thaw durability of Portland cement mortar/concrete can be more effectively enhanced by using air-entraining agent or water-repelling agent, and simutaneously by taking proper measures against foaming and/or the increased tendency of wide-pore building due to additive.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.957-960
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• 2006

In order to predict the accumulated damages by cyclic freeze-thaw, a regression analysis by the Response Surface Method (RSM) is used. RSM has merits when the other probabilistic simulation techniques can not guarantee the convergence of probability of occurrence or when the others can not differentiate the derivative terms of limit state functions, which are composed of random design variables in the model of complex system or the system having higher reliability. For composing limit state function, the important parameters for cyclic freeze-thaw-deterioration of concrete structures, such as water to cement ratio, entrained air pores, and the number of cycles of freezing and thawing, are used as input parameters of RSM. The predicted results of relative dynamic modulus and residual strains after 300 cycles of freeze-thaw for specimens show very good agreements with the experimental results. The RSM result can be used to predict the probability of occurrence for designer specified critical values. Therefore, it is possible to evaluate the life cycle management of concrete structures considering the accumulated damages by the cyclic freeze-thaw by the use of proposed prediction method.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.589-592
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• 2006

The durability of concrete involves resistance to freeze-thaw action, corrosion, permeation, carbonation, chemical attack and so on. Generally, properties of concrete have been well understood under the separate action of these deterioration mechanisms. However, in practice, the degradation of concrete usually is the result of combined action of physical and chemical attack and can be accelerated by the combined action of several deterioration mechanisms. In the present study, to evaluate the combined deterioration by freeze-thaw action and seawater attack, ground granulated blast-furnace slag or silica fume concrete with water or seawater as mixing water was exposed to 300 cycles of freeze-thaw action. Tests were conducted to determined the relative dynamic modulus of elasticity and compressive strength. Furthermore, The MIP analysis were performed on the deteriorated part of concrete due to freeze-thaw action and seawater attack.

• Journal of the Korea Concrete Institute
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• v.17 no.5 s.89
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• pp.857-868
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• 2005

This paper deals with the accumulated damage in concrete structures due to the cyclic freeze-thaw as an environmental load. The cyclic ice body nucleation and growth processes in porous systems are affected by the thermo-physical and mass transport properties, and gradients of temperature and chemical potentials. Furthermore, the diffusivity of deicing chemicals shows significantly higher value under cyclic freeze-thaw conditions. Consequently, the disintegration of concrete structures is aggravated at marine environments, higher altitudes, and northern areas. However, the properties of cyclic freeze-thaw with crack growth and diffusion of chloride ion effects are hard to be identified in tests, and there has been no analytic model for the combined degradations. The main objective is to determine the driving force and evaluate the reduced strength and stiffness by freeze-thaw. For the development of computational model of those coupled deterioration, micro-pore structure characterization, pore pressure based on the thermodynamic equilibrium, time and temperature dependent super-cooling with or without deicing salts, nonlinear-fracture constitutive relation for the evaluation of internal damage, and the effect of entrained air pores (EA) has been modeled numerically. As a result, the amount of ice volume with temperature dependent surface tensions, freezing pressure and resulting deformations, and cycle and temperature dependent pore volume has been calculated and compared with available test results. The developed computational program can be combined with DuCOM, which can calculate the early aged strength, heat of hydration, micro-pore volume, shrinkage, transportation of free water in concrete. Therefore, the developed model can be applied to evaluate those various practical degradation cases as well.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.3
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• pp.17-25
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• 2006

To increase freeze-thaw resistance of porous concrete, this study examined physical properties of polymer by replacing paste used as a binding material with polymer, using unsaturated polyester and epoxy resin, and changing the mixing ratio of polymer. According to the result of this study, when the mixing ratio of resin paste to aggregates was 11 to 16%, voids volume was 33 to 37% and unit weight was about 1620 to 1720kg/$m^3$. In comparison with previous studies using cement paste, voids volume increased by about 7 to 16%, while unit weight decreased by about 100 to 300kg/$m^3$. Compressive strength was 90 to 155kg/$cm^2$ at the age of 7 days, which was 5-40kg/$cm^2$ bigger than porous concrete using cement paste. From a viewpoint of freeze-thaw resistance, it was identified that pluse velocity fell by 0.23km/sec, about 7% of the original velocity, when the cycle of freeze-thaw was repeated 300 times. In spite of 300 repetitions of the cycle, relative dynamic modulus of elasticity was more than 60%, which suggested that its freeze-thaw resistance was more excellent compared with the result that relative dynamic modulus of elasticity of porous concrete using cement paste was 60 % or less under the condition of 80 repetitions of freeze-thaw cycle.

• Magazine of the Korean Society of Agricultural Engineers
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• v.22 no.3
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• pp.60-74
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• 1980

Soil-cement mixtures involve problems in it's durability in grain size distribution and mineral composition of the used soils as well as in cement content, compaction energy, molding water content, and curing. As an attempt to solve the problems associated with durability of weathered granite soil with cement treated was investigated by conducting tests such as unconfined compression test, it's moisture, immers, wet-dry and freeze-thaw curing, mesurement of loss of weight with wet-dry and freeze-thaw by KS F criteria and CBR test with moisture curing on the five soil samples different in weathering and mineral composition. The experimental results are summarized as follows; The unconfined compressive strength was higher in moisture curing rather than in the immers and wet-dry, while it was lowest in freeze-thaw. Decreasing ratio of unconfined compressive strength in soil-cement mixtures were lowest in optimum moisture content or in the dry side rather than optimum moisture content with freeze-thaw. The highly significant ceofficient was obtained between the cement content and loss of weight with freeze-thaw and wet-dry. It was possible to obtain the durability of soil-cement mixtures, as the materials of base for roads, containing above 4 % of cement content, above 3Okg/cm$_2$ of unconfined compressive trength with seven days moisture curing or 12 cycle of freeze-thaw after it, above 100% of relative unconfined compressive strength, 80% of index of resistance, below 14% of loss of weight with 12 cycle of wet-dry and above 1. 80g/cm$_2$ of dry density.