• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.3
• /
• pp.335-343
• /
• 2018

Objective: Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect the activity of the ESCs. Methods: Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition. The post-thaw survival of the ESCs was monitored after the post-treatment subculture and the ability of cell proliferation, reactive oxygen species (ROS) generation, apoptosis and mitochondrial ATP synthesis was subsequently examined. Results: Regardless of the subculture number, P19 ESCs showed better (p<0.05) doubling time and less ATP production than P4 ESCs and such difference was not influenced by fresh or cryopreservation. The difference between P4 and P19 ESC lines became decreased as the post-treatment subculture was increased and the six times subculture eliminated such difference. Similarly, transient but prominent difference in ROS production and apoptotic cell number was detected between P4 and P19 ESCs only at the 1st subculture after treatment, but no statistical differences between two ESC lines was detected in other observations. Conclusion: The results of this study suggest that post-thaw subculture of ESCs under the same environment is recommended for standardizing their cellular activity. The activity of cell proliferation ability and ATP synthesis can be used as parameters for quality control of ESCs.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.04a
• /
• pp.565-568
• /
• 2008

Domestic area of most be happened chloride deicer damage. Because daily mean temperature is below 0$^{\circ}C$ from the area of domestic most. Concrete durability influence Air Content. Presently, We used to AE(air-entraining agent) for increase freeze-thaw durability. So, on concrete Air Spacing ratio used $200{\mu}m{\sim}230{\mu}m$ in Canada and under $250{\mu}m$ in Japan institution. Use of Air content has been and will continue to be a major part of concrete durability and scaling. Chloride-containing chemicals such as calcium chloride or rock salt are main deicers for the road. The prepared optimum mix concrete in this study show that freeze-thaw and scaling resistance of Non-AE(air content 1.5%) and AE (air content 4.5%, 7.2%). Solution concentrations of deicing agent were good result, and the pore system and change of hydration products is not difference comparing before freeze-thaw test.

• Geomechanics and Engineering
• /
• v.18 no.2
• /
• pp.189-203
• /
• 2019

Brine leakage is a common phenomenon during construction facilitated by artificial freezing technique, threatening the stability of frozen wall due to the continual thawing of already frozen domain. This paper takes the frequently encountered soft clay in Wujiang District as the study object, and remolded specimens were prepared by mixing calcium chloride solutions at five levels of concentration. Both the deformation and pore water pressure of frozen specimens during thawing were investigated by two-stage loading tests. Three sections were noted from the changes in the strain rate of specimens during thawing at the first-stage load, i.e., instantaneous, attenuated, and quasi-stable sections. During the second-stage loading, the deformation of post-thawed soils is closely correlated with the dissipation of pore water pressure. Two characteristic indexes were obtained including thaw-settlement coefficient and critical water content. The critical water content increases positively with salt content. The higher water content of soil leads to a larger thaw-settlement coefficient, especially at higher salt contents, based on which an empirical equation was proposed and verified. The normalized pore water pressure during thawing was found to dissipate slower at higher salt contents, with a longer duration to stabilize. Three physical indexes were experimentally determined such as freezing point, heat conductivity and water permeability. The freezing point decreases at higher salt contents, especially as more water is involved, like the changes in heat conductivity. The water permeability maintains within the same order at the considered range of salt contents, like the development of the coefficient of consolidation. The variation of the pore volume distribution also accounts for this.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.10 no.2
• /
• pp.143-151
• /
• 2022

In this study the relative dynamic modulus and surface rebound hardness measurement methods were used for comparison to predict the occurrence of frost dam age on the concrete. From the test results, it was observed that the initiation of concrete dam age predicted by surface rebound hardness values was 200 cycles quicker than that of the relative dynamic modulus method in the W/C 70 specimens. In addition, it continuously provided data that showed the frost damage development of concrete surfaces according to increasing freeze-thaw cycles. This indicated that the frost dam age of the concrete could be found from the initial point of its occurrence by the surface rebound hardness measurement method. Similar results were also observed in W/C 60 and 50 specimens. Therefore, it is considered that surface rebound hardness method predicted the freeze-thaw damage well, regardless of water-cement ratio.

• Computers and Concrete
• /
• v.21 no.2
• /
• pp.149-156
• /
• 2018

This paper investigated the influence of binary and ternary blends of mineral admixtures in self-compacted mortar (SCM) on the fresh, mechanical and durability properties. For this purpose, 25 mortar mixtures were prepared having a total binder content of $640kg/cm^3$ and water/binder ratio between 0.41 and 0.50. All the mixtures consisted of Portland cement (PC), fly ash (FA) and silica fume (SF) as binary and ternary blends and air-entrained admixture wasn't used while control mixture contained only PC. The compressive and tensile strength tests were conducted for 28 and 91 days as well as slump-flow and V-funnel time tests whilst freeze-thaw (F-T) resistance and capillary water absorption tests were made for 91-day. Finally, in general, the use of SF with FA as ternary blends improved the tensile strength of mortars at 28- and 91-day while the use of SF15 with FA increased the compressive strength of the mortars compared to binary blends of FA. SCM mixtures with ternary blends had lower the sorptivity values than that of the mortars with binary blends of FA and the control mixture due to the beneficial properties of SF while the use of FA with SF as ternary blends induced the F-T resistance enhancement.

• Tunnel and Underground Space
• /
• v.13 no.3
• /
• pp.215-224
• /
• 2003

Weathering in nature was simulated by freeze-thaw cyclic test which represents mechanical weathering. Measured physical properties were elastic wave velocities, absorption rate, volume change and weight change. Uniaxial compression tests were also conducted before and after the weathering tests. The change in weight and volume of the specimens were not clearly related to the weathering process, but P, S wave velocities, uniaxial compression strength and Young's modulus were clearly decreased as weathering progresses. Test result can be used for the assessment of long-term stability of rock slopes.

• International Journal of Highway Engineering
• /
• v.16 no.5
• /
• pp.29-38
• /
• 2014

PURPOSES : The purpose of this study is to evaluate moisture susceptibility of a dense graded and an open graded asphalt mixtures by the method of AASHTO T-283. METHODS : To simulate moisture damage of asphalt pavements with continuously rainfall during summer rainy season, the dense graded and the open graded asphalt mixtures were immersed in water for 15 days and were measured the weight and the change of strength. Also, the mixtures were performed five freeze-thaw cycles to simulate moisture damage of the mixtures by freeze-thaw during winter and were measured the change of strength. The degradation characteristics model was used to analyze the relationship between strength and moisture damage. RESULTS : According to the results, the dense graded and the open graded asphalt mixtures were shown in the similar trends of the strength changes by immersion time and freeze-thaw cycle. However, the moisture damage reduction of open graded asphalt mixture was more sensitive in early phase than that of dense graded asphalt mixture.

• Food Science and Biotechnology
• /
• v.18 no.4
• /
• pp.991-995
• /
• 2009

As a principle ingredient in omija-eui, the physicochemical properties of mung bean starch (MBS) paste were investigated and compared to those of rice and corn starch. The amylose and the protein content of MBS were higher than those of rice or corn starch while the total sugar content and the swelling power of MBS were lower. In addition, the clarity of MBS paste was higher than either rice or corn starch paste. Regarding pasting properties, the peak viscosity and cool paste viscosity of MBS were higher than those of either rice or corn starch. During the freeze-thaw cycle, MBS exhibited higher degree of syneresis than corn and rice starch, which decreased with high starch concentration and heating temperature. The paste properties and freeze-thaw stability of MBS showed a potential for improving the quality of omija-eui.

• International Journal of Highway Engineering
• /
• v.17 no.2
• /
• pp.47-53
• /
• 2015

PURPOSES: The scaling of a concrete surface caused by the combined effects of frost and de-icing salts is one of the main reasons for the need to repair transportation infrastructures in cold-climate regions. This study describes the results of attempts to determine the scaling resistance of concrete incorporating mineral admixtures such as fly ash, GGBFS, and silica fume, and subjected to the actions of frost and salt. METHODS : Conventionally, to evaluate the fundamental properties of concrete, flexural and compressive strength measurements are regularly performed. Based on the ASTM C 672 standard, concrete is subjected to 2%, 4%, and 8% $CaCl_2$ salt solutions along with repeated sets of 50 freeze/thaw cycles, and the scaling resistance was evaluated based on the mass of the scale and a visual examination. RESULTS : It was observed that silica fume is very effective in enhancing the scaling resistance of concrete. Meanwhile, concrete incorporating GGBFS exhibited poor resistance to scaling, especially in the first ten freeze/thaw cycles. However, fly ash concrete generally exhibited the maximum amount of damage as a result of the frost-salt attack, regardless of the concentrations of the solutions. CONCLUSIONS: It can be concluded that the scaling resistance of concrete is highly dependent on the type of the mineral admixture used in the concrete. Therefore, to provide a durable concrete pavement for use in cold-climate regions, the selection of a suitable binder is essential.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2009.05a
• /
• pp.261-262
• /
• 2009

The prevent methods of Alkali-Silica Reaction (ASR) are studying after the failure cases by ASR were reported in Korea. In this study, the freeze-thaw test and scaling test for premixed fly ash cement were performed. The ratio of fly ash and cement is 20 percent and 80 percent by weight of total cementious material. The results show that the dynamic modulus after 300 cycles the freeze-thaw test for most of specimen except the specimen have less 3% air was more than 90 % and the loss of weigh the specimen after 50 cycles scaling test was less than 1kg/$m^3$.