• Genomics & Informatics
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• v.8 no.4
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• pp.177-184
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• 2010

The target of rapamycin (TOR) signaling pathway is a conserved pathway that regulates eukaryotic cell growth in response to environmental cues. Chemical genomic approaches that profile rapamycin sensitivity of yeast deletion strains have given insights into the function of TOR signaling pathway. In the present study, we analyzed the rapamycin sensitivity of yeast deletion library strains on synthetic medium. As a result, we identified 130 strains that are hypersensitive or resistant to rapamycin compared with wild-type cells. Among them, 36 genes are newly identified to be related to rapamycin sensitivity. Moreover, we found 16 strains that show alteration in rapamycin sensitivity between complex and synthetic media. We suggest that these genes may be involved in part of TOR signaling activities that is differentially regulated by media composition.

• Language and Information
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• v.19 no.1
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• pp.75-101
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• 2015

This study investigated the distribution of subject properties in Korean. Based on the previous studies on so-called 'subjecthood diagnostics' in Korean Multiple Subject Constructions (MSCs), the current study investigates how different subject properties are distributed in single subject constructions (Non-MSCs) and MSCs. Focusing on two distinct subject properties - obligatory control (OC) of PRO and coordinated deletion (CD) - an empirical syntactic experiment was designed to test how these two subjecthood diagnostics behave in the Non-MSC and MSC environments. Seventy Korean native speakers were tested over magnitude estimation task, composed of 80 Korean sentences representing different subject properties in single or multiple subject constructions. Overall results showed (i) both OC and CD can be used as subjecthood diagnostic tests in the Non-MSC environments, but (ii) that only OC can be used as a subjecthood diagnostic test in the MSC environments.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1288-1294
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• 2009

We have used mutational analysis to identity four genes, MXAN3553, MXAN3554, MXAN3555, and MXAN3556, constituting an operon that is essential for normal fruiting body development in Myxococcus xanthus. Deletion of MXAN3553, which encoded a hypothetical protein, resulted in delayed fruiting body development. MXAN3554 was predicted to encode a metallopeptidase, and its deletion caused fruiting body formation to fail. Inactivation of MXAN3555, which encoded a putative NtrC-type response regulator, resulted in delayed aggregation and a severe reduction in sporulation. Fruiting bodies also failed to develop with the deletion of MXAN3556, another gene encoding a hypothetical protein.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.245-249
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• 1994

An amino-terminal 14 amino acids deletion and three site-directed mutations were created to investigate the mechanism of induction and repression of MerR regulatory protein in R100 mer operon from gramnegative Shigella flexneri. The amino-terminal 14 amino acids deletion, Cysl17Ser, and Cys126Ser mutations abolished the inducibility of the mer operon and the Hisl18Ala mutation resulted in the reduction of inducibility (about 9.1 % remaining) in complementation experiment in the presence of $Hg^{2＋}$ at subtoxic level ($1\mu M$). The complementation experiment with $Hg^{2＋}$ absent showed that Hisl18Ala, Cys126Ser, and wild-type MerR could repress the operon but Cysl17Ser could not, and the amino-terminal deletion mutant could neither induce nor repress the R100 mer operon.

• Proceedings of the KSPS conference
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• 2003.05a
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• pp.48-51
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• 2003

The purpose of this paper is to research the realization of /h/ between sonorant sounds. For this purpose, we analyze speech of 5 people using standard Korean. As a result, we can find that the possibility of deletion of /h/ is increasing, when speech rate is high, the AP has more syllables, and /h/ is far from the AP-initial. While the position of AP or IP has no relation to realization of /h/. The deletion of /h/ is more often in this order. Followed segments: lateral>nasal>vowel, following segments: vowel>glide. And there is no change on duration of following vowel after /h/ deletion.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1716-1721
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• 2003

Steroid hormones and their receptors play an important role in reproductive process. Estrogen is intimately involved with pregnancy and its function is mediated through the estrogen receptor which has been chosen as a candidate gene to study litter size in pigs. In this study, we report that two estrogen receptor variants, designated pER-1 and pER-2 were co-expressed in the uteri of normal cycling Lan-Yu pig (Sus vittatus; a small-ear miniature in Taiwan) with the pER-1 expression level appeared to be several times higher than that of pER-2. These receptor variants were isolated using reverse transcription-PCR from the pig uteri and their sequences were determined. The pER-1 and pER-2 sequences, which are homologous to those found in other mammalian estrogen receptors, encode putative proteins consisting of 574 and 486 amino acids, respectively. A deletion in exon I was identified in both sequences, with deletion lengths of 63 bp in pER-1 and 327 bp in pER-2. The deletion in pER-1 is internal to that in pER-2 and both deletions resulted in a truncation of the B domain, which confers the transactivating activity of estrogen receptor protein. This result describes the existence of estrogen receptor variants with a deletion in exon I and implies the possibility that physiological functioning of an estrogen receptor may not require the presence of an intact B domain.

• ETRI Journal
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• v.43 no.5
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• pp.835-855
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• 2021

With the recent increase in data disclosure, the Comprehensive Knowledge Archive Network (CKAN), which is an open-source data distribution platform, is drawing much attention. CKAN is used together with additional extensions, such as Datastore and Datapusher for data management and Harvest and DCAT for data collection. This study derives the problems of CKAN itself and Harvest Extension. First, CKAN causes two problems of data inconsistency and storage space waste for data deletion. Second, Harvest Extension causes three additional problems, namely source deletion that deletes only sources without deleting data themselves, job stop that cannot delete job during data collection, and service interruption that cannot provide service, even if data exist. Based on these observations, we propose herein an improved CKAN that provides a new deletion function solving data inconsistency and storage space waste problems. In addition, we present an improved Harvest Extension solving three problems of the legacy Harvest Extension. We verify the correctness and the usefulness of the improved CKAN and Harvest Extension functions through actual implementation and extensive experiments.

• Korean Journal of Microbiology
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• v.49 no.4
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• pp.419-423
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• 2013

Three genes, nsdD, nsdC, and veA are known to be necessary for positive regulating sexual development of Aspergillus nidulans. Since the mutants of those genes hardly form fruiting bodies in heterokaryons constructed by cross between two of them, it is difficult to isolate double mutants. In this work, double mutants of ${\Delta}nsdD$ ${\Delta}veA$ and ${\Delta}nsdD$ ${\Delta}nsdC$ were isolated using the characteristic of the nsdD deletion mutant that it could develop mature cleistothecia in hypoxic and low temperature culture condition. According to the phenotypes of double mutants, the nsdD gene controls the apical growth independently with veA or nsdC. Deletion of veA or nsdC was epistatic to nsdD deletion for pigment production. Conidia formation in submerged culture with lactose as sole carbon source was observed in ${\Delta}nsdD$ ${\Delta}nsdC$ double mutant implicating it to be unique phenotype of nsdC deletion.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1491-1495
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• 2004

Small Tail Han Sheep has significant characteristics of high prolificacy and non-seasonal ovulatory activity and is an excellent local sheep breed in P. R. China. Recently a novel member of the transforming growth factor $\beta$ (TGF$\beta$) superfamily termed bone morphogenetic protein 15 (BMP15) was shown to be specifically expressed in oocytes and to be essential for female fertility. Therefore, BMP15 is a candidate gene for reproductive performance of Small Tail Han Sheep. The whole genomic nucleotide sequence of BMP15 gene in Small Tail Han Sheep was searched for polymorphisms by PCR-SSCP and direct sequencing, and only one polymorphism was found. The polymorphism was a result of a 3 base pair deletion, which eliminated a single Leu codon (CTT). The allelic frequencies for A (without deletion) and B (with a codon deletion) are 0.73 and 0.27 respectively. The effects of BMP15 genotype on litter size were evaluated using the least squares model. This indicated that there was a significant association between litter size of Small Tail Han Sheep and a deletion in BMP15 gene (p=0.02<0.05). Small Tail Han Sheep ewes with AA and AB genotype produce on average 0.5 and 0.3 more lambs per litter than those ewes with BB genotype.

• Journal of Genetic Medicine
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• v.12 no.1
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• pp.57-60
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• 2015

CHARGE syndrome (coloboma, heart defects, atresia choanae, retarded growth and development, genital hypoplasia, and ear abnormalities) is characterized by multiple malformations and is diagnosed using distinct consensus criteria. Mutations in the gene encoding chromodomain helicase DNA-binding protein 7 (CHD7) are the major cause of CHARGE syndrome. Clinical features of CHARGE syndrome considerably overlap those of 22q11.2 deletion syndrome. Of these features, immunodeficiency and hypocalcemia are frequently reported in patients with 22q11.2 deletion syndrome but are rarely reported in patients with CHARGE syndrome. In this report, we have described the case of a patient with typical phenotypes of 22q11.2 deletion syndrome but without the proven chromosome microdeletion. Mutation analysis of CHD7 identified a pathogenic mutation (c.2238+1G>A) in this patient. To our knowledge, this is the first case of CHARGE syndrome with immunodeficiency and hypocalcemia in Korea. Our observations suggest that mutation analysis of CHD7 should be performed for patients showing the typical phenotypes of 22q11.2 deletion syndrome but lacking the proven chromosome microdeletion.