• Genomics & Informatics
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• v.21 no.4
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• pp.47.1-47.12
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• 2023

Silver barb (Barbonymus gonionotus) is among the most economically important freshwater fish species in Thailand. It ranks fourth in economic value and third in production weight for fisheries and culture in Thailand. An XX/XY sex-determination system based on gynogenesis was previously reported for this fish. In this study, the molecular basis underlying the sex-determination system was further investigated. Genome-wide single-nucleotide polymorphism data were generated for 32 captive-bred silver barb individuals, previously scored by phenotypic sex, to identify sex-linked regions associated with sex determination. Sixty-three male-linked loci, indicating putative XY chromosomes, were identified. Male-specific loci were not observed, which indicates that the putative Y chromosome is young and the sex determination region is cryptic. A homology search revealed that most male-linked loci were homologous to the Mariner/Tc1 and Gypsy transposable elements and are probably the remnants of an initial accumulation of repeats on the Y chromosome from the early stages of sex chromosome differentiation. This research provides convincing insights into the mechanism of sex determination and reveals the potential sex determination regions in silver barb. The study provides the basic data necessary for increasing the commercial value of silver barbs through genetic improvements.

• Journal of Plant Biotechnology
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• v.50
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• pp.190-199
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• 2023

Date palm (Phoenix dactylifera L.: Arecaceae) is a dioecious species where only female trees bear fruits. In their natural state, date palms produce dates once a year. However, in Thailand, some trees were observed to produce dates during the off-season, despite no variations in morphology. The availability of such off-season fruits can significantly increase their market value. Interestingly, most female off-season date palms investigated in this study were obtained through micropropagation. Hence, there is an urgent need for genetic markers to distinguish female offseason flowering plantlets within tissue culture systems. In this study, we aimed to develop random amplification of polymorphic DNA-sequence characterized amplified region (RAPD-SCAR) markers for the identification of female off-season flowering date palms cultivated in Thailand. A total of 160 random decamer primers were employed to screen for specific RAPD markers in off-season flowering male and female populations. Out of these, only one primer, OPN-02, generated distinct genomic DNA patterns in female off-season flowering (FOFdp) individuals compared to female seasonal flowering genotypes. Based on the RAPD-specific sequence, specific SCAR primers denoted as FOFdpF and FOFdpR were developed. These SCAR primers amplified a single 517-bp DNA fragment, predominantly found in off-season flowering populations, with an accuracy rate of 60%. These findings underscore the potential of SCAR marker technology for tracking offseason flowering in date palms. Notably, a BLAST analysis revealed a substantial similarity between the SCAR marker sequence and the transcript variant mRNA from Phoenix dactylifera encoding the SET DOMAIN GROUP 40 protein. In Arabidopsis, this protein is involved in the epigenetic regulation of flowering time. The genetic potential of the off-season flowering traits warrants further elucidation.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.403-415
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• 2023

This study aimed to isolate and identify L-(+)-lactic acid-producing bacteria from tree barks collected in Thailand and evaluate the potential strain as probiotics. Twelve strains were isolated and characterized phenotypically and genotypically. The strains exhibited a rod-shaped morphology, high-temperature tolerance, and the ability to ferment different sugars into lactic acid. Based on 16S rRNA gene analysis, all strains were identified as belonging to Weizmannia coagulans. Among the isolated strains, BKMTCR2-2 demonstrated exceptional lactic acid production, with 96.41% optical purity, 2.33 g/l of lactic acid production, 1.44 g/g of lactic acid yield (per gram of glucose consumption), and 0.0049 g/l/h of lactic acid productivity. This strain also displayed a wide range of pH tolerance, suggesting suitability for the human gastrointestinal tract and potential probiotic applications. The whole-genome sequence of BKMTCR2-2 was assembled using a hybridization approach that combined long and short reads. The genomic analysis confirmed its identification as W. coagulans and safety assessments revealed its non-pathogenic attribute compared to type strains and commercial probiotic strains. Furthermore, this strain exhibited resilience to acidic and bile conditions, along with the presence of potential probiotic-related genes and metabolic capabilities. These findings suggest that BKMTCR2-2 holds promise as a safe and effective probiotic strain with significant lactic acid production capabilities.

• Tuberculosis and Respiratory Diseases
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• v.87 no.3
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• pp.349-356
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• 2024

Background: Acute pulmonary embolism (APE) is a fatal disease with varying clinical characteristics and imaging. The aim of this study was to define the clinical characteristics, risk factors, and outcomes in patients with APE at a university hospital in Thailand. Methods: Patients diagnosed with APE and admitted to our institute between January 1, 2017 and December 31, 2022 were retrospectively enrolled. The clinical characteristics, investigations, and outcomes were recorded. Results: Over the 6-year study period, 369 patients were diagnosed with APE. The mean age was 65 years; 64.2% were female. The most common risk factor for APE was malignancy (46.1%). In-hospital mortality rate was 23.6%. The computed tomography pulmonary artery revealed the most proximal clots largely in segmental pulmonary artery (39.0%), followed by main pulmonary artery (36.3%). This distribution was consistent between survivors and non-survivors. Multivariate logistic regression analysis revealed that APE mortality was associated with active malignancy, higher serum creatinine, lower body mass index (BMI), and tachycardia with adjusted odds ratio (95% confidence interval [CI]) of 3.70 (1.59 to 8.58), 3.54 (1.35 to 9.25), 2.91 (1.26 to 6.75), and 2.54 (1.14 to 5.64), respectively. The prediction model was constructed with area under the curve of 0.77 (95% CI, 0.70 to 0.84). Conclusion: The overall mortality rate among APE patients was 23.6%, with APE-related death accounting for 5.1%. APE mortality was associated with active malignancy, higher serum creatinine, lower BMI, and tachycardia.

• Safety and Health at Work
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• v.15 no.3
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• pp.345-351
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• 2024

Background: A decrease in physical fitness with age can impact work ability. Exploring the role of physical fitness is important for identifying interventions to enhance work ability among aging workers. We sought to determine the association between physical fitness and physically demanding work ability in aging workers. Methods: We recruited workers aged 45-65 years from eight departments of a university hospital in Bangkok, Thailand. Work ability was assessed using the work ability index (WAI), and physical fitness components were evaluated through various tests. Associations between work ability and physical fitness were examined using a multivariable regression analysis. Results: The response rate was 46.4% (n = 216). The mean WAI score was 41.6. Participants with an overweight or obese body mass index (BMI) had 1.8 (95% CI -3.1, -0.4) points lower WAI scores than did those within a healthy BMI range. A handgrip strength test revealed that participants in the good/very good group had 2.5 (95% CI: 0.6, 4.3) higher WAI scores than did those in the very poor/poor group. For a single-leg stance test, participants in the above-average group had 2.1 (95% CI: 0.7, 3.5) points greater WAI scores than did those in the below-average group. No significant associations were found between work ability and 3-min step, chair stand, or sit-and-reach test scores. Conclusion: The overall work ability of the participants was good. Body composition, muscle strength, and balance were associated with work ability. Promoting physical fitness is a viable strategy for enhancing work ability among aging workers.

• Industrial Engineering and Management Systems
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• v.13 no.3
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• pp.342-355
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• 2014

Organizational dedication to corporate social responsibility (CSR) is set off by a number of driving forces, such as enhancing corporate financial performance, concern for the environment, regulatory compliance, and social equity. This article aims to ascertain the uptake of CSR among firms in the Philippines and Thailand leading to the attainment of sustainable development. The results revealed that CSR is carried out by the companies primarily to enhance their competitive advantage and legal compliance, which are deemed essential to the firms' existence. This study seeks to contribute to understanding how companies undertake their operations based on socially responsible practices so that the theoretical conceptualizations of sustainability can be developed. By determining what prods companies to pursue CSR, it will shed light on the mechanism that promotes the existence of sustainable organizations, linking it with CSR and the companies' objectives and strategies.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1110-1115
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• 2004

Water covers two-thirds of the earth and has a great influence on the future existence of all human being. Thailand has extensive coastline and near shore water that contain vast biological and mineralogical resources. The rivers and canals can be found around the country especially in the Bangkok, which once called the Venice of the East. Autonomous underwater robot (AUR) will be soon a tool to help us better understand water resources and other environmental issues. This paper presents the design and basic control of a six-degree of freedom AUR "Chalawan", which was constructed to be used as a testbed for shallow. It is a simple low cost open-frame design, which can be modified easily to supports various research areas in the underwater environment. It was tested with a conventional proportional-integral-derivative (PID) controller. After fine-tuning of the controller gains, the results showed the controller's good performances. In the future, the dynamic model of the robot will be analyzed and identified. The advanced control algorithm will be implemented based on the obtained model.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1017-1022
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• 2004

This paper presents the conversion speed drive of the cooling tower fans from constant to variable speed. The speed of fan is adjusted using variable speed drives. Since the ambient temperature of cooling tower is varied seasonally, an economic evaluation was performed to determine the potential annual savings. The performances of the proposed technique were observed using cooling tower fans of chemical plant in Thailand as an illustrative case study. The experimental results demonstrating the energy savings fork cooling process and some economic benefits are obtained.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2309-2312
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• 2003

This paper presents the minimization of the energy costs based on energy saving for industrial retort process of canned food. The approved proposed method is related the optimal steam or hot water flow control to achieve desired temperature of retort process. The smooth response and zero steady state error can be also achieved. The performances of the proposed control technique were observed using a small tuna canned food plant in Thailand as an illustrative example. The experimental results are given to support the saving in energy costs and some benefits of the proposed technique.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.480-483
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• 2004

This paper, we present the evaluation of outage probability on the SNR under the overlapped carrier allocation scheme in the reverse link with imperfect power control of a cellular CDMA system, which is base on using Gaussian approximation. In numerical results, the band limited pulse generated by square-root of raised consin pulse shaping filters (SRRC) of the transmitted signal is investigated and compared in conventional FD/CDMA and OC-CDMA of the outage probability. It will be also show that the outage probability can be improved by overlapping of carriers. We use error statistics to model the intra-cell interference and evaluate the impact of different mobiles velocity and number of resolvable paths on the system performance.