• Parasites, Hosts and Diseases
• /
• 제56권3호
• /
• pp.247-257
• /
• 2018

The aim of this study was to conduct a freshwater snail survey in Mae Lao agricultural basin to assess the diversity with a focus on habitat types and their larval trematode infections. Snails were collected and examined in 14 sites of Mae Lao agricultural basin from August 2016 to October 2017. A total of 1,688 snail individuals were collected and classified into 7 families, 8 genera, and 12 species. Snail diversity and habitat types were higher in rice paddies than irrigation canals and streams. The most abundant species was Bithynia siamensis siamensis, representing 54.6% of the sample. Three species of snails act as first intermediate host were found with cercarial infections. They were Filopaludina sumatrensis polygramma, B. s. siamensis, and Melanoides tuberculata. The cercariae were categorized into 7 types; echinostome, monostome, gymnocephalous, virgulate, parapleurolophocercous, pleurolophocercous and megalurous cercariae. Parapleurolophocercous cercariae constituted the most common type of cercariae recovered, contributing 41.2% of all infections in snails. Echinostome metacercariae infections were found in 6 snail species with 7.6% prevalence. In addition, the metacercaria of avian trematode, Thapariella sp. were found in Filopaludina spp. snails and B. funiculata with a prevalence of 0.5%. This is the first report for Thapariella metacercariae in the snail host, B. funiculata, and also confirmed that viviparid and bithyniid snails act as the second intermediate hosts of this trematode. This work will provide new information on the distribution and intermediate host of trematode in this area.

• 한국균학회지
• /
• 제15권3호
• /
• pp.158-168
• /
• 1987

1. Cassava 전분(澱粉)을 이용(利用)하여 곰팡이를 $28^{\circ}C$에서 96시간(時間) 액체정치(液體定置) 배양(培養)한 바 배지중(培地中)의 Cassava 전분함량(澱粉含量)이 6%, 72시간(時間)일 때에 균체량(菌體量)이 가장 많았다. 2. Cassava 전분(澱粉)이 6% 함유(含有)된 배지(培地)에서 곰팡이를 배양(培養)하여 얻은 균체단백질량(菌體蛋白質量)은 Rhizopus는 48시간(時間) 배양(培養)하였을때 가장 많았고, Aspergillus niger 및 Aspergillus fumigatus에서는 72시간(時間) 배양(培養)했을 때 균체단백질량(菌體蛋白質量)이 많았다. 3. Cassava 전분량(澱粉量)을 4%, 6% 및 8%되게 변화(變化)시킨 배지(培地)에서 분리균(分離菌)을 접종(接種)하여 96시간(時間) 배양(培養)하면서 24시간(時間)마다 배양여액(培養濾液) 중(中)의 m-amylase의 활성(活性)을 측정(測定)한 바 8% 농도(濃度)에서 Aspergillus niger 및 Aspergillus fumigatus는 72시간(時間)일 때 높은 D.P 값을 얻었고. Rhizopus 는 144시간(時間)일 때 D.P 값이 높았다.

• 한국작물학회지
• /
• 제46권4호
• /
• pp.334-340
• /
• 2001

Two different types of molecular markers, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP), were used to measure genetic diversity among five Korean, eight Thai, and three wild soybeans. For SSR analysis, a total of 20 markers were surveyed to detect polymorphisms. For SNP analysis, four primers were designed from consensus sequence regions on disease resistance protein homolog genes, and used to amplify the genomic region. The PCR products were sequenced. A number of polymorphic SSR and SNP bands were scored on all genotypes and their genetic similarity was measured. Clustering analysis was performed independently on both types of markers. Clustering based on SSR markers separated the genotypes into three main groups originated from Korea, Thailand, and wild soybeans. On the other hand, two main groups were classified using SNP analysis. It seemed that SSR was more informative than SNP in this study. This may be due to the fact that SNP was surveyed on the smaller genomic region than SSR. Grouping based on the combined data of both markers revealed similar results to that of SNP rather than that of SSR. This might be due to the fact that more loci from SNP were considered to measure genetic relatedness than those from the SSR.

• Nutrition Research and Practice
• /
• 제11권2호
• /
• pp.130-138
• /
• 2017

BACKGROUND/OBJECTIVES: Low consumption of fruit and vegetable is frequently viewed as an important contributor to obesity risk. With increasing childhood obesity and relatively low fruit and vegetable consumption among Thai children, there is a need to identify the determinants of the intake to promote fruit and vegetable consumption effectively. SUBJECTS/METHODS: This cross-sectional study was conducted at two conveniently selected primary schools in Nakhon Pathom. A total of 609 students (grade 4-6) completed questionnaires on personal and environmental factors. Adequate fruit and vegetable intakes were defined as a minimum of three servings of fruit or vegetable daily, and adequate total intake as at least 6 serves of fruit and vegetable daily. Data were analyzed using descriptive statistics, the chi-square test, and multiple logistic regression. RESULTS: The proportion of children with a sufficient fruit and/or vegetable intakes was low. Covariates of child's personal and environmental factors showed significant associations with sufficient intakes of fruit and/or vegetable (P < 0.05). Logistic regression analyses showed that the following factors were positively related to sufficient intake of vegetable; lower grade, a positive attitude toward vegetable, and fruit availability at home; and that greater maternal education, a positive child's attitude toward vegetable, and fruit availability at home were significantly associated with sufficient consumption of fruits and total fruit and vegetable intake. CONCLUSIONS: The present study showed that personal factors like attitude toward vegetables and socio-environmental factors, such as, greater availability of fruits were significantly associated with sufficient fruit and vegetable consumption. The importance of environmental and personal factors to successful nutrition highlights the importance of involving parents and schools.

• 공학교육연구
• /
• 제5권1호
• /
• pp.68-76
• /
• 2002

It is proposed to raise the debate on Engineering and Technical Education at the global economic level and to examine some of the issues facing developing and poorer countries in managing and improving the quality of engineering education in their countries, especially in the context of internet and IT culture After the fall of the Berlin Wall, the world is now divided in two realigned blocks: one of developed(rich or advanced) countries which have a social security safety net for their population and another of developing(or poor) countries which have no such luxuries for their population. For the general public in the developing countries, any engineering or technical degree/diploma is a passport to lifelong wellbeing of an individual and his/her extended family. Therefore, the demand for such qualifications is very high and it is almost a rat race amongst school leavers to get into engineering/technical colleges. In view of this booming demand, there are hundreds of privately funded engineering/technical colleges in countries like Philippines, India, Thailand, etc., besides state funded ones. It is extremely difficult to ensure good quality in this mushrooming scenario. There are also many very small poorly resourced developing countries where there is only one engineering school and/or two-three technical colleges. Products of these schools/colleges work only in their own country and education globalization have little or no meaning for them. Besides highlighting the aforementioned general issues, the Paper also presents a few case studies on problems of accreditation and quality assessment in larger developing countries like India and the Philippines. The Paper also discusses the effects of commercialization on the quality of education and social impacts of IT revolution on educational processes.

• Imaging Science in Dentistry
• /
• 제43권4호
• /
• pp.289-293
• /
• 2013

Purpose: This study was performed to determine the relationship between the stage of tooth eruption (both vertical and mesio-angular) and chronological age. Materials and Methods: Indirect digital panoramic radiographs were used to measure the distances from the dentinoenamel junction (DEJ) of the second molars to the occlusal plane of the second molar teeth and of the adjacent third molars in 264 Thai males and 437 Thai females using ImageJ software. The ratio of those distances was calculated by patient age, and the correlation coefficient of the ratio of the third molar length to the second molar length was calculated. Results: The correlation between the height of the vertically erupted upper third molar teeth and age was at the intermediate level. The age range of ${\geq}15$ to <16 years was noted to be the range in which the correlation between the chronological age determined from the eruptional height and actual chronological age was statistically significant. The mean age of the female subjects, in which the position of the right upper third molar teeth was at or above the DEJ of the adjacent second molar but below one half of its coronal height was $19.9{\pm}2.6$ years. That for the left side was $20.2{\pm}2.7$ years. The mean ages of the male subjects were $20.1{\pm}3.3$ years and $19.8{\pm}2.7$ years for the right and left sides, respectively. Conclusion: It might be possible to predict chronological age from the eruption height of the wisdom teeth.

• Safety and Health at Work
• /
• 제9권2호
• /
• pp.159-163
• /
• 2018

Background: Fatigue and sleepiness are inter-related and common among road transport drivers. In this study, sleep deprivation and fatigue among chemical transportation drivers were examined. Methods: A cross-sectional study surveying 107 drivers from three hazardous types of chemical production and transportation industries (nonflammable gases, flammable gases, and flammable liquids) was conducted. Data on sleep deprivation were collected using questionnaires of the Stanford Sleeping Scale and the Groningen Sleep Quality Scale. Fatigue was assessed using an interview questionnaire and a flicker fusion instrument. Results: Chemical drivers had a mean sleeping scale (Stanford Sleeping Scale) of 1.98 (standard deviation 1.00) and had a mean score of 1.89 (standard deviation 2.06) on the Groningen Sleep Quality Scale. High-risk drivers had higher scores in both the Stanford Sleeping Scale and the Groningen Sleep Quality Scale with a mean score of 2.59 and 4.62, respectively, and those differences reached statistical significance (p < 0.05). The prevalence of fatigue, as assessed through a critical flicker fusion analyzer, subjective fatigue question, and either of the instruments, was 32.32%, 16.16%, and 43.43%, respectively. Drivers who slept <7 hours and had poor sleep quality were found to have more fatigue than those who slept enough and well. Drivers who had a more sleepiness score resulted in significantly more objective fatigue than those who had a less sleepiness score. Conclusion: Sleep quality and sleeping hour can affect a driver's fatigue. Optimization of work-rest model should be considered to improve productivity, driver retention, and road safety.

• Safety and Health at Work
• /
• 제5권2호
• /
• pp.86-90
• /
• 2014

Background: Urinary 1-hydroxypyrene (1-OHP) was selected as a biomarker of polycyclic aromatic hydrocarbons (PAHs) to explore the accumulation level in the bodies of workers at rubber smoke sheet factories in southern Thailand. Methods: Spot urine samples were taken from four groups of workers from June 2006 to November 2007. The nonexposure or control groups included habitual cigarette smokers and nonsmokers. The other two groups were workers exposed to particle-bound PAHs from rubber wood smoke and they were nonsmokers. All spot urine samples were analyzed for 1-OHP and creatinine levels. Results: The mean${\pm}$standard deviation urinary 1-OHP in the control group of habitual smokers and the nonsmokers was $0.24{\pm}0.16{\mu}mol/mol$ creatinine and not-detected to $0.14{\mu}mol/mol$ creatinine, respectively. In the workers, the 1-OHP levels on workdays had no significant difference from the 1-OHP levels on the days off. The yearly average 1-OHP level was $0.76{\pm}0.41{\mu}mol/mol$ creatinine whereas the average 1-OHP level during 10 consecutive workdays was $1.06{\pm}0.29{\mu}mol/mol$ creatinine (p > 0.05). Conclusion: The urinary 1-OHP levels of workers exposed to PAHs were high. The accumulation of 1-OHP in the body was not clear although the workers had long working hours with few days off during their working experience. Therefore, a regular day off schedule and rotation shift work during high productive RSS should be set for RSS workers.

• Journal of Microbiology and Biotechnology
• /
• 제30권11호
• /
• pp.1670-1679
• /
• 2020

The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.

• Archives of Pharmacal Research
• /
• 제29권1호
• /
• pp.80-87
• /
• 2006

Leukemias are common worldwide. Wilms'tumor1 (WT1) protein is highly expressed in leukemic blast cells of myeloid and lymphoid origin. Thus, WT1 mRNA serves as a tumor marker for leukemias detection and monitoring disease progression. Curcumin is well known for its anticancer property. The objective of this study was to investigate the effect of curcumin on WT1 gene expression in patient leukemic cells. The leukemic cells were collected from 70 childhood leukemia patients admitted at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, in the period July 2003 to February 2005. There were 58 cases of acute lymphoblastic leukemia (ALL), 10 cases of acute myeloblastic leukemia (AML), and 2 cases of chronic myelocytic leukemia (CML). There were 41 males and 29 females ranging from 1 to 15 years old. Leukemic cells were cultured in the presence or absence of 10 mM curcumin for 48 h. WT1 mRNA levels were determined by RT-PCR. The result showed that curcumin reduced WT1 gene expression in the cells from 35 patients (50%). It affected the WT1 gene expression in 4 of 8 relapsed cases (50%), 12 of 24 cases of drug maintenance (50%), 7 of 16 cases of completed treatment (44%), and 12 of 22 cases of new patients (54%). The basal expression levels of WT1 gene in leukemic patient cells as compared to that of K562 cells were classified as low level (1-20%) in 6 of 20 cases (30%), medium level (21-60%) in 12 of 21 cases (57%), and high level (61-100%) in 17 of 23 cases (74%). In summary, curcumin decreased WT1 mRNA in patient leukemic cells. Thus, curcumin treatment may provide a lead for clinical treatment in leukemic patients in the future.