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Genetic diversity and population genetic structure of Cambodian indigenous chickens

  • Ren, Theary;Nunome, Mitsuo;Suzuki, Takayuki;Matsuda, Yoichi
    • Animal Bioscience
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    • v.35 no.6
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    • pp.826-837
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    • 2022
  • Objective: Cambodia is located within the distribution range of the red junglefowl, the common ancestor of domestic chickens. Although a variety of indigenous chickens have been reared in Cambodia since ancient times, their genetic characteristics have yet to be sufficiently defined. Here, we conducted a large-scale population genetic study to investigate the genetic diversity and population genetic structure of Cambodian indigenous chickens and their phylogenetic relationships with other chicken breeds and native chickens worldwide. Methods: A Bayesian phylogenetic tree was constructed based on 625 mitochondrial DNA D-loop sequences, and Bayesian clustering analysis was performed for 666 individuals with 23 microsatellite markers, using samples collected from 28 indigenous chicken populations in 24 provinces and three commercial chicken breeds. Results: A total of 92 haplotypes of mitochondrial D-loop sequences belonging to haplogroups A to F and J were detected in Cambodian chickens; in the indigenous chickens, haplogroup D (44.4%) was the most common, and haplogroups A (21.0%) and B (13.2%) were also dominant. However, haplogroup J, which is rare in domestic chickens but abundant in Thai red junglefowl, was found at a high frequency (14.5%), whereas the frequency of haplogroup E was considerably lower (4.6%). Population genetic structure analysis based on microsatellite markers revealed the presence of three major genetic clusters in Cambodian indigenous chickens. Their genetic diversity was relatively high, which was similar to findings reported for indigenous chickens from other Southeast Asian countries. Conclusion: Cambodian indigenous chickens are characterized by mitochondrial D-loop haplotypes that are common to indigenous chickens throughout Southeast Asia, and may retain many of the haplotypes that originated from wild ancestral populations. These chickens exhibit high population genetic diversity, and the geographical distribution of three major clusters may be attributed to inter-regional trade and poultry transportation routes within Cambodia or international movement between Cambodia and other countries.

The Outcomes of an e-Wellness Program for Lupus Patients in Thailand: A Participatory Action Research Approach

  • Nop T. Ratanasiripong;Steven Cahill;Christine Crane;Paul Ratanasiripong
    • Journal of Preventive Medicine and Public Health
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    • v.56 no.2
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    • pp.154-163
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    • 2023
  • Objectives: Systemic lupus erythematosus (SLE) or lupus patients usually experience various physical and psychological challenges. Since the coronavirus disease 2019 pandemic, these challenges have become even harsher. Using the participatory action research approach, this study evaluated how an e-wellness program (eWP) impacted SLE-related knowledge and health behaviors, mental health, and quality of life among lupus patients in Thailand. Methods: A 1-group, pretest-posttest design study was conducted among a purposive sample of lupus patients who were members of Thai SLE Foundation. The 2 main intervention components were: (1) online social support, and (2) lifestyle and stress management workshops. Sixty-eight participants completed all the study requirements, including the Physical and Psychosocial Health Assessment questionnaire. Results: After being in the eWP for 3 months, participants' mean score for SLE-related knowledge increased significantly (t=5.3, p<0.001). The increase in sleep hours was statistically significant (Z=-3.1, p<0.01), with the percentage of participants who slept less than 7 hours decreasing from 52.9% to 29.0%. The percentage of participants reporting sun exposure decreased from 17.7% to 8.8%. The participants also reported significantly lower stress (t(66)=-4.4, p<0.001) and anxiety (t(67)=-2.9, p=0.005). The post-eWP quality of life scores for the pain, planning, intimate relationship, burden to others, emotional health, and fatigue domains also improved significantly (p<0.05). Conclusions: The overall outcomes showed promising results of improved self-care knowledge, health behaviors, mental health status, and quality of life. It is recommended that the SLE Foundation continues to use the eWP model to help the lupus patient community.

1D Kinetics Model of NH3-Fed Solid Oxide Fuel Cell (암모니아 공급 고체산화물 연료전지의 1D 반응 모델)

  • VAN-TIEN GIAP;THAI-QUYEN QUACH;KOOK YOUNG AHN;YONGGYUN BAE;SUNYOUP LEE;YOUNG SANG KIM
    • Journal of Hydrogen and New Energy
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    • v.33 no.6
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    • pp.723-732
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    • 2022
  • Cracking ammonia inside solid oxide fuel cell (SOFC) stack is a compact and simple way. To prevent sharp temperature fluctuation and increase cell efficiency, the decomposition reaction should be spread on whole cell area. This leading to a question that, how does anode thickness affect the conversion rate of ammonia and the cell voltage? Since the 0D model of SOFC is useful for system level simulation, how accurate is it to use equilibrium solver for internal ammonia cracking reaction? The 1D model of ammonia fed SOFC was used to simulate the diffusion and reaction of ammonia inside the anode electrode, then the partial pressure of hydrogen and steam at triple phase boundary was used for cell voltage calculation. The result shows that, the ammonia conversion rate increases and reaches saturated value as anode thickness increase, and the saturated thickness is bigger for lower operating temperature. The similar cell voltage between 1D and 0D models can be reached with NH3 conversion rate above 90%. The 0D model and 1D model of SOFC showed similar conversion rate at temperature over 750℃.

Corrosion Protection Properties of Co3O4 and CoFe2O4 Nanoparticles for Water-Based Epoxy Coatings on 2024-T3 Aluminum Alloys

  • Thu Thuy Thai;Anh Truc Trinh;Thi Thanh Tam Pham;Hoan Nguyen Xuan
    • Corrosion Science and Technology
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    • v.22 no.2
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    • pp.90-98
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    • 2023
  • In this study, cobalt oxide (Co3O4) and cobalt-doped magnetite (CoFe2O4) nanoparticles were synthesized by a hydrothermal method. They were then used as corrosion inhibitors for corrosion protection of AA2024-T3 aluminum alloys. These obtained nanoparticles were characterized by x-ray diffraction, field-emission scanning electron microscopy, and Zeta potential measurements. Corrosion inhibition activities of Co3O4 and CoFe2O4 nanoparticles were determined by performing electrochemical measurements for bare AA2024-T3 aluminum alloys in 0.05 M NaCl + 0.1 M Na2SO4 solution containing Co3O4 or CoFe2O4 nanoparticles. Corrosion protection for AA2024-T3 aluminum alloys by a water-based epoxy with or without the synthesized Co3O4 or CoFe2O4 nanoparticles was investigated by electrochemical impedance spectroscopy during immersion in 0.1 M NaCl solution. The corrosion protection of epoxy coating deposited on the AA2024-T3 surface was improved by incorporating Co3O4 or CoFe2O4 nanoparticles in the coating. The corrosion protection performance of the epoxy coating containing CoFe2O4 was higher than that of the epoxy coating containing Co3O4.

First-Principles Investigation on the Electromechanical Properties of Monolayer 1H Pb-Dichalcogenides

  • Nguyen Hoang Linh;Nguyen Minh Son;Tran The Quang;Nguyen Van Hoi;Vuong Thanh;Do Van Truong
    • Korean Journal of Materials Research
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    • v.33 no.5
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    • pp.189-194
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    • 2023
  • This study uses first-principles calculations to investigate the mechanical properties and effect of strain on the electronic properties of the 2D material 1H-PbX2 (X: S, Se). Firstly, the stability of the 1H Pb-dichalcogenide structures was evaluated using Born's criteria. The obtained results show that the 1H-PbS2 material possesses the greatest ideal strength of 3.48 N/m, with 3.68 N/m for 1H-PbSe2 in biaxial strain. In addition, 1H-PbS2 and 1H-PbSe2 are direct semiconductors at equilibrium with band gaps of 2.30 eV and 1.90 eV, respectively. The band gap was investigated and remained almost unchanged under the strain εxx but altered significantly at strains εyy and εbia. At the fracture strain in the biaxial direction (19 %), the band gap of 1H-PbS2 decreases about 60 %, and that of 1H-PbSe2 decreases about 50 %. 1H-PbS2 and 1H-PbSe2 can convert from direct to indirect semiconductor under the strain εyy. Our findings reveal that the two structures have significant potential for application in nanoelectronic devices.

Improvement of rooster semen freezability and fertility rate after sericin supplementation in freezing semen extender

  • Ruthaiporn Ratchamak;Supakorn Authaida;Wuttigrai Boonkum;Vibuntita Chankitisakul
    • Animal Bioscience
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    • v.36 no.10
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    • pp.1530-1535
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    • 2023
  • Objective: Semen cryopreservation result in decreased sperm parameters and fertilization ability. Sericin exhibits antioxidant activity by reducing lipid peroxidation resulting from free radicals, which can potentially improve cryopreservation outcomes. The present study aimed to examine the efficacy of various sericin concentrations supplemented with a rooster semen-freezing extender on post-thaw semen quality and fertilizing ability of sperm after cryopreservation. Methods: Semen samples were collected from 40 roosters (5 reps), then were pooled, and divided into four groups by the levels of sericin supplementation (0%, 0.25%, 0.50%, and 0.75%) in a freezing extender. Semen suspensions were loaded in medium straw (0.5 mL) and cryopreserved with the traditional liquid nitrogen vapor method. Post-thawed semen was evaluated for sperm motility, sperm viability, and lipid peroxidation. Also, the fertility test was determined. Results: The results showed that supplementation of the freezing extender with 0.50% to 0.75% sericin resulted in greater total motility and progressive motility and lower malondialdehyde levels than the other groups after cryopreservation (p<0.05). However, the viability of 0.75% decreased compared with the value of 0.50% sericin supplementation (p<0.05). Moreover, the fertility and hatchability of total eggs were significantly higher in the 0.50% sericin group than in the other groups (p<0.05). Conclusion: In conclusion, 0.50% sericin is recommended as an alternative component of the freezing extender to improve cryopreserved rooster semen.

Patch loading resistance prediction of steel plate girders using a deep artificial neural network and an interior-point algorithm

  • Mai, Sy Hung;Tran, Viet-Linh;Nguyen, Duy-Duan;Nguyen, Viet Tiep;Thai, Duc-Kien
    • Steel and Composite Structures
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    • v.45 no.2
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    • pp.159-173
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    • 2022
  • This paper proposes a hybrid machine-learning model, which is called DANN-IP, that combines a deep artificial neural network (DANN) and an interior-point (IP) algorithm in order to improve the prediction capacity on the patch loading resistance of steel plate girders. For this purpose, 394 steel plate girders that were subjected to patch loading were tested in order to construct the DANN-IP model. Firstly, several DANN models were developed in order to establish the relationship between the patch loading resistance and the web panel length, the web height, the web thickness, the flange width, the flange thickness, the applied load length, the web yield strength, and the flange yield strength of steel plate girders. Accordingly, the best DANN model was chosen based on three performance indices, which included the R^2, RMSE, and a20-index. The IP algorithm was then adopted to optimize the weights and biases of the DANN model in order to establish the hybrid DANN-IP model. The results obtained from the proposed DANN-IP model were compared with of the results from the DANN model and the existing empirical formulas. The comparison showed that the proposed DANN-IP model achieved the best accuracy with an R^2 of 0.996, an RMSE of 23.260 kN, and an a20-index of 0.891. Finally, a Graphical User Interface (GUI) tool was developed in order to effectively use the proposed DANN-IP model for practical applications.

Morphotypes of the apical constriction of maxillary molars: a micro-computed tomographic evaluation

  • Jeffrey Wen-Wei Chang;Kuzhanchinathan Manigandan;Lakshman Samaranayake;Chellapandian NandhaKumar;Pazhamalai AdhityaVasun;Johny Diji;Angambakkam Rajasekharan PradeepKumar
    • Restorative Dentistry and Endodontics
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    • v.47 no.2
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    • pp.19.1-19.15
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    • 2022
  • Objectives: The aim of this study was to evaluate and compare the apical constriction (AC) and apical canal morphology of maxillary first and second molars, using micro-computed tomography (micro-CT). Materials and Methods: The anatomical features of 313 root canals from 41 maxillary first molars and 57 maxillary second molars of patients with known age and sex were evaluated using micro-CT, with a resolution of 26.7 ㎛. The factors evaluated were the presence or absence of AC, the morphotypes, bucco-lingual dimension, mesio-distal dimension, and the profile (shape) of AC and the apical root canal. The apical root canal dimensions, location of the apical foramen (AF), AC to AF distance, and presence of accessory canals in the apical 5 mm were also assessed. Descriptive and analytical statistics were used for data evaluation. Results: AC was present in all 313 root canals. Patients' age and sex did not significantly impact either AC or the apical canal dimensions. The most common AC morphotype detected was the traditional (single) constriction (52%), followed by the parallel (29%) morphotype. The mean AC dimensions in maxillary first molars were not significantly different from those in maxillary second molars. Sixty percent of AF were located within 0.5 mm from the anatomic apex. Conclusions: The most common morphotype of AC detected was the traditional constriction. Neither patients' age nor sex had a significant impact on the dimensions of the AC or the apical root canal. The majority of AF (60%) were located within 0.5 mm from the anatomic apex.

Poem in Ca Trù: Type, Structure, Content (베트남의 음악시, 까쭈: 형식, 구조, 내용)

  • Nguyen, Duc Mau
    • SUVANNABHUMI
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    • v.2 no.1
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    • pp.95-110
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    • 2010
  • Poem plays an important role in Ca trù. Many music researchers say that singing Ca trù is singing poem. Of 46 tunes of Ca trù, there are more than 10 tunes expressed in available poems or styles of poetry; for example: in the tune Tỳ bà, the performer could sing Tỳ bà hành by Bạch Cư Dị being converted into seven-seven-six-eight-word-meter; in the reciting poem tunes, just reciting 5 Thien thai poems by Tào Đường or 3 Thanh Bình tune poems by Lý Bạch; in the reciting poetic essay (phú), reciting Tien Xich Bich and Hau Xich Bich by Tô Đông Pha. Others like bắc phản, cung bắc sometimes used six-eight word meters. Structurally, those are available for familiar types and beyond the scope of particular creativity because they do not originate from Ca trù's activity environment like recitative. Recitative is the main tune of Ca trù and has become an independent poem type. In terms of literature, recitative has a particular form structure and special type content. Unlike other tunes of Ca trù that only stop at some fixed works, recitative has increased to thousands of works in quantity and has been composed for many centuries. For those reasons, we confined ourselves the research to the creation which is the most typical of Ca tru: The recitative.

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Aluminum in rocks: Optimized microwave-assisted acid digestion and UV-Vis spectrophotometric measurement

  • Nguyen Thanh-Nho;Thai Huynh-Thuc;Le-Thi Anh-Dao;Do Minh-Huy;Le-Thi Huynh-Mai;Le Quang-Huy;Nguyen-Thi Kim-Sinh;Nguyen Cong-Hau
    • Analytical Science and Technology
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    • v.36 no.5
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    • pp.216-223
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    • 2023
  • Aluminium (Al) is one of the major elements in rocks and its concentration can be varied, depending on different rock types as well as sources. The present study aimed to propose an analytical method based on the UV-Vis as a cheap, simple, and common instrument equipped in most laboratories for Al quantification in rocks after the microwave assisted acid digestion. The aluminone and 8-hydroxyquinoline were investigated for the colorimetric assay. The results show that the 8-hydroxyquinoline reagent was more favorable in terms of the minimized affects of the potential interferences present in the digested solutions, i.e., Fe3+, Si4+ and F-. The calibration curve was constructed from 0.10 mg/L to 3.00 mg/L with the goodness of linearity (R2 = 0.9996). The limits of detection and quantification (LOD and LOQ) were estimated, i.e., 0.029 mg/L and 0.087 mg/L, respectively. The 8-hydroxyquinoline was applied to real rock samples, demonstrating favorable precision (RSD = 0.34 %-1.8 %) and no remarkable differences were found compared to the inductively coupled plasma-mass spectrometry (ICP-MS) as a reference measurement approach.