• Title/Summary/Keyword: tetracycline-HCl

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Stability of Tetracycline Hydrochloride in Reverse Micelles

  • Kim, Hyun-Joo;Lee, Hwa-Jeong;Sah, Hong-Kee
    • Journal of Pharmaceutical Investigation
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    • v.35 no.5
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    • pp.333-336
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    • 2005
  • The objective of this study was to investigate the stability of tetracycline HCl on encapsulation into and inside reverse micelles. To do so, tetracycline HCl was first mixed with cetyltrimethylammonium bromide, water and ethyl formate to make reverse micelles. The degradation kinetics of tetracycline HCl inside the reverse micelles was then assessed by scrutinizing its stability data. Under our experimental conditions, the reverse micelles formed spontaneously in absence of any mixing devices. During the preparation of the reverse micelles, however, considerable portions of tetracycline HCl underwent a chemical reaction (e.g., epimerization). For instance, $51.4{\pm}0.6%$ of an initial concentration of tetracycline HCl was transformed into a degradation product. Once dissolved inside the reverse micelles, the degradation of tetracycline HCl followed an exponential decay pattern. The plot of log{the degradation rate of tetracycline HCl} versus log{tetracycline HCl concentration} made it possible to determine the order of degradation reaction and rate constant. It was proven that the degradation of tetracycline HCl inside the reverse micelles followed a first order kinetics with a rate constant of 0.0027 $hour^{-1}$. Meriting further investigation might be formulation studies to stabilize tetracycline HCl on encapsulation into and inside the reverse micelles.

Effect of Application of Tetracycline - HCl on Implant Surface;Scanning Electron Microscopic Study (염산테트라싸이클린이 임플란트 표면에 미치는 효과;주사전자현미경적 연구)

  • Hong, Jung-Ah;Herr, Yeek;Lee, Man-Sup;Park, Joon-Bong
    • Journal of Periodontal and Implant Science
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    • v.31 no.2
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    • pp.333-344
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    • 2001
  • The present study was performed to evaluate the effect of tetracycline - HCl on the change of implant surface microstructure according to application time. Implants with pure titanium machined surface and titanium plasma-sprayed surface were utilized. Implant surface was rubbed with 50mg/ml tetracycline - HCl solution for 1 min., $1\frac{1}{2}\;min$., 2 min., $2\frac{1}{2}\;min$. and 3min. respectively in the test group and with saline for 1 min. in the control group. Then, the specimens were processed for scanning electron microscopic observation. The following results were obtained. 1. Pure titanium machined surfaces conditioned with saline for 1 min., showed a few shallow grooves and ridges and were less irregular. 2. Pure titanium machined surfaces conditioned with 50mg/ml tetracycline - HCl solution showed more irregular and corrosive surfaces compared to the control group irrespective of the application time of tetracycline - HCl solution. 3. Titanium plasma-sprayed surfaces conditioned with saline for 1 min., were deposited with round amorphous particles and showed round or irregular pores that can be connected to each other. 4. The irregularity of titanium plasma-sprayed surfaces conditioned with 50mg/ml tetracycline - HCl solution was lessened and the flattened areas were wider relative to the application time of tetracycline - HCl solution. 5. Titanium plasma-sprayed surfaces conditioned with tetracycline - HCl solution for 1 min. and $1\frac{1}{2}\;min$. were very similar to that of the control group, but the surfaces conditioned for 2 min., $2\frac{1}{2}\; min$. and 3 min. were changed. In conclusion, pure titanium machined surfaces were changed irrespective of the application time of tetracycline - HCl solution. And titanium plasma-sprayed surfaces conditioned with tetracycline - HCl solution began to be changed from $1\frac{1}{2}\;min$. This results will be applicable to the regenerative procedures for peri-implantitis treatment.

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Effect of Tetracycline-HCL in Root Conditioning;A SEM Study (염산테트라싸이클린을 이용한 치근면처리의 효과)

  • Kim, Eun-Jung;Heer, Yeek;Lee, Man-Sup;Kwon, Young-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.30 no.1
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    • pp.121-134
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    • 2000
  • Root conditioning has introduced to dissolve the smear layer and to produce surface demineralization, resulting to exposure of dentin collagen fibril and opening of dentinal tubules. The purpose of the present study was to examine the effect of different concentration and application time of tetracycline-HCL on root conditioning. Total 40 root specimen were prepared from 20 periodontitis-prone human single rooted tooth. The specimen were treated with tetracycline-HCL solution(20mg/ml, 50mg/ml, 100mg/ml)for 20 sec, 3 min, 5 min., and saline for 30 sec. The application method was rubbing method with cotton pellet. Under the scanning electron microscopy(20KV), the extent of smear removal and opening of the dentinal tubules were examined at x 3000. The following results were obtained. 1. Treatment of root specimen with saline did not remove the smear layer and open the dentinal tubules. 2. Treatment of root specimen with different concentration of tetracycline-HCL for 20 sec also did not remove the smear layer completely. 3. Treatment of root specimen with different concentration of tetracycline-HCL for 3 min opened the dentinal tubules and removed smear layer. 4. Treatment of root specimen with 50mg/ml of tetracycline-HCL for 3 min showed collagen fibril within the opened dentinal tubules. In conclusion, the effect of root conditioning with tetracycline-HCL is more dependent on the application time than the application concentration. Root conditioning with 50mg/ml tetracycline-HCL for 3 min is enough for obtaining the periodontal regeneration.

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EXPERIMENTAL STUDIES OF THE EFFECT OF TETRACYCLINE ON GROWTH OF TIBIA AND MANDIBLE IN RATS (Tetracycline이 백서의 경골 및 하악골발육에 미치는 영향에 관한 실험적 연구)

  • Lee, Mi Dae
    • The korean journal of orthodontics
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    • v.5 no.1
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    • pp.33-41
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    • 1975
  • To study the effect of the tetracycline-HCl on the growth of the rats, the author made a daily injection of tetracycline-HCl in the amount of 40mg/kg of body weight to the lactating mother and weanling rats for 7 days. Lactating and weanling young rats were killed at a week intervals and removed tibia and mandible from soft tissues. The effect of the tetracycline on the growth of rats were analysed from the weight, the length of tibia and the size of mandible. The results were as follows: 1. Tetracycline inhibited the growth of young rats. 2. Inhibitory effects were greater ill the weanling group than the lactating group. 3. The inhibitory effect was marked just after the injection of tetracycline-HCl in body weight and after a week in mandible and tibia. 4. The inhibitory effect of growth nab continued for 2-3 weeks in body weight and 3-4 weeks in tibia and mandible. 5. In growth of mandible, the antero-posterior growth was more inhibited than the vertical.

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The effect of conditioning by Tetracycline-Hcl on implant surface;The SEM study and. surface roughness measurements : RBM surface (염산 테트라싸이클린이 RBM적용 임프란트 표면구조에 미치는 영향의 미세구조 및 표면 거칠기 변화에 관한 연구)

  • Lim, Hae-Soo;Park, Joon-Bong;Kwon, Young-Hyuk;Herr, Yeek;Chung, Jong-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.37 no.3
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    • pp.585-597
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    • 2007
  • The present study was performed to evaluate the effect of Tetracycline-HCI and Saline on the change of implant surface microstructure and surface roughness according to application time. Implants with resorbable blasting media surface were utilized. Before test all 13 implants were measured surface roughness. Among them, 6 implants were rubbed with 50mg/ml Tetracycline-HCl solution and other 6 implants with saline for $\frac{1}{2}$min., 1min., $1\frac{1}{2}$min., 2min., $2\frac{1}{2}$min and 3min. Then, specimens were processed for scanning electron microscopic observation and surface roughness after test. The results of this study were as follows. 1. Control group showed a few irregular, rough, uneven surface with crater-like depression. 2. The test group with Tetracycline-HCl conditioning showed an altered surface when Tetracycline-HCl was applied for 30secs, and showed a various surface alteration as application times go on. 3. The test group with Saline conditioning showed no significant surface differences and surface roughness. 4. The significant increase of Ra value was showed when Tetracycline-HCl was applied for 30secs. In conclusion, the 50mg/ml Tetracycline-HCl must not be applied for the RBM surface implant for surface treatment.

Clinical Study of Tetracycline HCl Conditioning in the Treatment of Gingival Recession. A Comparative Study (치근 피개 술식시 치근에 도포된 테트라사이클린의 효과에 관한 임상적 연구)

  • Kim, Jong-Ae;Chung, Chin-Hyung;Lim, Sung-Bin
    • Journal of Periodontal and Implant Science
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    • v.30 no.1
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    • pp.133-146
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    • 2000
  • Histological studies indicate a tetracycline HCl similar to citric acid to induce connetive tissue repair in animals. When tetracycline HCl was used as a root conditioning agent in humans, there was a trend toward more connective tissue attachment than in root planing alone. The purpose of this study was to evaluate clinical effect of tetracycline HCl in the treatment of gingival recession. 44 teeth in 12 patients with bilaterally gingival recession & Miller classification I, II gingival recession were selected and 22 teeth were treated with 125mg/ml tetracycline HCl , the others was not treated with tetracycline HCl. Gingival recession, pocket depth, clinical attachment level, width of keratinized gingiva were observed at baseline, postoperative 4, 12, 20weeks. Both groups were statistically analyzed by Wilcoxon signed Ranks Test & Wilcoxon's rank sum test(Mann-whitney test) using SPSS program.(5% significance level) The results were as follows: 1........The change of gingival recession, clinical attachment level, keratinized gingiva in both groups were increased significantly at 4, 12, 20 weeks. 2.......The pocket depth exhibited no marked changes throughout the entire investigation in both groups. 3........The change of gingival recession in tetracycline group was increased significantly than control group at 4, 12, 20 weeks and the percentage of root coverage was 93% in tetracycline group and 83% in control group. 4........The change of clinical attachment level, pocket depth, keratinized tissue from baseline to 4, 12, 20 weeks was not differ significanltly in both group.

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Micromorphometric change of implant surface conditioned with tetracycline-HCI;HA and oxidized surface (표면처리 시간에 따른 임플란트 미세구조의 변화;HA와 양극산화 표면 임플란트)

  • An, Sang-Ho;Park, Joon-Bong;Kwon, Young-Hyuk;Herr, Yeek;Chung, Jong-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.35 no.4
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    • pp.891-905
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    • 2005
  • The present study was performed to evaluate the effect of tetracycline-HCL on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface. HA-coated surface and TiUniteTM surface were utilized. Implant surface was rubbed with 50mg/ml tetracycline-HCL solution for $\frac{1}{2}min.$, 1min., $1\frac{1}{2}min.$, 2min., and $2\frac{1}{2}min.$ respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follows. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. In HA-coated surfaces, round particles were deposited irregularly. The roughness of surfaces conditioned with tetracycline-HCL was lessened and the cracks were increased relative to the application time. 3. The anodic oxidized surfaces showed the craterous structures. The surface conditioning with tetracycline-HCl didn't influence on its micro-morphology. In conclusion, the detoxification with 50mg/ml tetracycline-HCL must be applied respectively with different time according to various implant surfaces.

In vitro Antimicrobial Activity in Combination of Antibacterials Against Fish-pathogenic Bacteria (병용 항균제의 어류질병 세균에 대한 시험관내 항균활성)

  • Jung, Sung-Hee;Kim, Jin-Woo
    • Journal of fish pathology
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    • v.13 no.1
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    • pp.45-51
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    • 2000
  • Bacterial diseases with mixed infection have recently occurred at land-based flounder farms in Korea. Thus, single antibacterial is not effective for therapy of mixed bacterial diseases of fish because of their different causative bacteria. The purpose of the present study was to obtain basic data for positive usefulness of a combination of antibacterials used for synergism to mixed bacterial diseases of fish. Snergistic interaction in combination of antibacterials was determined by in vitro antimicrobial activity against selected fish-pathogenic bacteria, Vibrio anguillarum, Edwardsiella tarda, Streptococcus sp., Staphylococcus epidermidis, on the basis of Checkerboard assay using fractional inhibitory concentration (FIC) indices. Synergistic interactions were observed in combinations of (oxytetracycline HCL+lincomycin), (tetracycline HCL+florfenicol), (oxytetracycline HCL+florfenicol) against V. anguillarum, (sodium nifurstyrenate+florfenicol), (tetracycline HCL+florfenicol), (sodium nifurstyrenate+oxolinic acid), (oxytetracycline HCL+florfenicol) against E. tarda, (ciprofloxacine+oleandomycin), (oxytetracycline HCL+oleandomycin), (tetracycline HCL+oleandomycin), (oxytetracycline HCL+lincomycin), (oxytetracycline HCL+spiramycin), (oxytetracycline HCL+erythromycin), (doxycycline HCL+oleandomycin), (tetracycline HCL+spiramycin) against Streptococcus sp., and (ciprofloxacine+erythromycin), (florfenicol+erythromycin), (doxycycline HCL+oleandomycin), (ciprofloxacine+oleandomycin) against S. epidermidis.

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Scanning Electron Microscopic Study of the Effect of Tetracycline-HCl on the Change of Implant Surface Microstructure according to Application Time (염산테트라싸이클린의 적용시간에 따른 임플란트 표면변화에 관한 주사전자현미경적 연구)

  • Kim, Woo-Young;Lee, Man-Sup;Park, Joon-Bong;Herr, Yeek
    • Journal of Periodontal and Implant Science
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    • v.32 no.3
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    • pp.523-537
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    • 2002
  • The present study was performed to evaluate the effect of tetracycline - HCl on the change of implant surface microstructure according to application time. Implants with pure titanium machined surface, SLA surface and $TiO_2blasted$ surface were used. Implant surface was rubbed with 5Omg/ml tetracycline - HCl solution for ${\frac}{1}{2}$ min., 1 min., $1{\frac}{1}{2}$ min., 2 min., and 3min. respectively in the test group and with no conditioning in the control group. Then, the specimens were processed for scanning electron microscopic observation. The following results were obtained. 1. In the pure titanium machined surfaces, the control specimen showed a more or less rough machined surface composed of alternating positive and negative lines corresponding to grooves and ridges. After treatment, machining line was more pronounced for the control specimens. but in general, test specimens were similar to control. 2. In the SLA surfaces, the control specimen showed that the macro roughness was achieved by large-grit sandblasting. subsequently, the acid-etching process crated the micro roughness, which thus was superimposed on the macro roughness. 3. In the SLA surfaces, irrespective of the application time of 50mg/ml tetracycline-HCl solution, in general, test specimens were similar to control. 4. In the $TiO_2blasted$ surfaces the control specimen showed the rough surface with small pits. The irregularity of the $TiO_2blasted$ surfaces with 50mg/ml tetracycline-HCl solution was lessened and the flattened areas were wider relative to the application time of tetracycline - HCl solution. In conclusion, pure titanium machined surfaces and SLA surfaces weren't changed irrespective of the application time of tetracycline-HCl solution. And the $TiO_2blasted$ surfaces conditioned with tetracycline - HCl solution began to be changed from $1{\frac}{1}{2}$ min. This results are expected to be applied to the regenerative procedures for peri-implantitis treatment.

Radiosterilization of Medical Products(II) (전이방사선을 이용한 의료제품 멸균연구 II)

  • 이강순;천기정;김기수
    • Korean Journal of Microbiology
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    • v.13 no.2
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    • pp.64-70
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    • 1975
  • As a serial experiment of radiosterilization of medical products, we investigated the feasibility of using gamma ray to sterilize antibiotics, tetracycline HCl. Tetracycline HCl in aqueous solution and dried state irradiated with several dose levels was assayed for physico-chemical properties by checking UV absorption spectra, paper chromatogrph. IR spectra and NMR sepctra, and antibiotic activities by [means of agar plaque technique and tube $d_1$]ution method. In physico-chemical propertis and biological activities, the teracycline HCl in dried state remained radioresistant up to exposure of 10 Mrad, however, in aqueous solution, the dose of 300 Krad produced a significant inactivation. From the results of this study, radiosterilization of tetracycline HCl is more recommendable in the dried state than in aqueous solution.

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