• Title/Summary/Keyword: testosterone.

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Effects of Caponization and Testosterone on Bone and Blood Parameters of SCWL Male Chickens

  • Chen, Kuo-Lung;Tsay, Shiow-Min;Lo, Dan-Yuan;Kuo, Feng-Jui;Wang, Jiann-Hsiung;Chiou, Peter Wen-Shyg
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.706-710
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    • 2007
  • This study was to investigate the caponization effects on bone characteristics in male chickens, and the optimum testosterone implantation dosage on bone characteristics improvement. Healthy Single Comb White Leghorn cockerels were caponized at 12-wk-old and selected at 16-wk-old for a 10-wk feeding experiment. Fifteen intact male and caponized male chickens (capon) respectively were assigned to trial 1. Ten sham-operated chickens and 40 capons (randomly allocated into four treatments) were implanted with cholesterol (1.62 mm i.d., 3.16 mm o.d., $9.24{\pm}0.36$ mg), low (1 mm i.d., 3 mm o.d., $5.88{\pm}0.23$ mg), medium (1.62 mm i.d., 3.16 mm o.d., $9.81{\pm}0.17$ mg) or high dose (2 mm i.d., 4 mm o.d., $16.7{\pm}0.24$ mg) of testosterone in trial 2. The results from trial 1 showed that the tibia length, relative tibia weight, breaking strength, bending moment and stress in intact males were higher than capons (p<0.05). The blood phosphorus concentration in capons was higher than the intact male chickens (p<0.05). Caponization also resulted in more antrums and osteoclasts within periosteum and cortical bone from histological observation. In trial 2, the adverse impact of caponization on the bone breaking strength, bending moment and stress could be alleviated through medium dose testosterone implantation. It appears that caponization reduced androgen secretion hence influenced the biomechanical characteristics of bone (tibia) and these adverse effects could be alleviated through appropriate dose of testosterone implantation.

Effect of Testosterone on Free Radical Generating Enzyme and Lipid Peroxidation (지질과산화 반응과 Free Radical 생성계 효소활성에 미치는 Testosterone의 영향)

  • Huh, Keun;Shin, Uk-Seob;Park, Jong-Min
    • YAKHAK HOEJI
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    • v.38 no.2
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    • pp.166-173
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    • 1994
  • Sex hormones not only regulate external sexual characteristics but several internal biochemical processes. It is well accepted that life-span of female is longer than that of male. Life-span is closely related with aging process in which free radicals are known to be involved. We investigated the effect of testosterone on free radical generating systems and lipid peroxidation based on the sexual difference. Lipid peroxide levels of male and female mouse were increased proportionately with age, especially in male mouse. Increase in enzyme activity of aldehyde oxidase with age was observed in male mouse, while no siginificant change in enzyme activity was found in female mouse. Enzyme activity of xanthine oxidase also showed similar results. It, however, was not significant statistically. Lipid peroxide level and xanthine oxidase type conversion ratio of male and female mouse liver homogenate incubated at $37^{\circ}C$, increased remarkably in proportion to incubation time, especially in male mouse. Lipid peroxide level and aldehyde oxidase activity were measured in normal male mouse, castrated mouse and testosterone treated-castrated mouse. Castrated mouse group showed decrease in lipid peroxide level and aldehyde oxidase activity compared with normal group. Treatment of castrated mouse with testosterone, however turned the level of lipid peroxide and aldehyde oxidase activity back to normal. From the above results, it might be concluded that testosteron could increase the activities of free radical generating enzymes which would result in the formation of lipid peroxide, consequently leading to aging.

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Serum Testosterone Levels in Patients with Post-Traumatic Stress Disorder (외상 후 스트레스 장애 환자의 혈청 테스토스테론치)

  • Kim, Dong Su;Kim, Hae Jung;Bang, Yu Jin;Go, Chang Min;Chung, Moon Yong;Kang, Suk Hoon
    • Anxiety and mood
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    • v.9 no.2
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    • pp.106-112
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    • 2013
  • Objective : Several reports have found abnormal levels of androgen in post-traumatic stress disorder (PTSD) patients. This abnormality in androgen is hypothesized to due to chronic psychological stress effects on the hypothalamic-pituitary-gonadal (HPG) system. The present study was conducted to estimate serum testosterone levels in PTSD patients in comparison with normal subjects. Methods : Seventy-five male Korean veterans of the Vietnam War volunteered for the study, of which eleven were excluded because of incomplete psychological assessment. To measure basal serum testosterone, blood samples were collected between 8.00 and 9.30 a.m. The clinician administered PTSD scale (CAPS), the structured clinical interview for DSM-IV (SCID), Hamilton Anxiety Rating Scale (HAM-A), Hamilton Rating Scale for Depression (HAM-D), Mini International Neuropsychiatric Interview Plus (Korean version of MINI-Plus), CES-K (Korean version of combat exposure scale). Results : The serum testosterone level of PTSD patients ($5.4{\pm}2.5ng/mL$) was higher than that of a control group ($3.1{\pm}1.7ng/mL$, p<0.001). Testosterone levels were significantly correlated with CAPS (r=.38, p<.01), HAM-A (r=.35, p<.01) and HAM-D (r=.28, p<.01) in all subjects. Conclusion : The results of the present study suggest that chronic psychological stress affects the HPG system.

Effect of Spring and Summer on Semen Characteristics, Frozen-Thawed Sperm Viability and Testosterone Concentration in Yorkshire Boars (요크샤 종모돈의 정액성상, 동결-융해 후 정자의 생존성 및 테스토스테론의 농도에 미치는 봄과 여름의 영향)

  • 김홍기;임재삼;명평근;양창범;이영주;고현진;박창식
    • Korean Journal of Animal Reproduction
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    • v.25 no.3
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    • pp.201-206
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    • 2001
  • This study was carried out to investigate the effects of spring (March~May) and summer (June~August) influencing semen characteristics, frozen-thawed sperm viability and serum testosterone concentration in Yorkshire boars. Results of this study were as follows: 1. There were no significant differences in the semen volume, pH and sperm concentration of sperm-poor fraction of Yorkshire boars between spring and summer. However, sperm concentrations of sperm-rich fractions in spring were higher than those in summer (P<0.05). 2. Sperm motility and normal acrosome of raw semen in Yorkshire boars did not differ significantly between spring and summer, However, motility and normal acrosome of frozen-thawed sperm were higher in spring season than in summer season (P<0.05). 3. Serum testosterone concentrations in Yorkshire boars were 4.04 ng/$m\ell$ in spring and 2.85 ng/$m\ell$ in summer. Serum testosterone concentrations in spring were higher than those in summer (P<0.05). 4. In conclusion, when serum testosterone concentrations in Yorkshire boars were higher, frozen-thawed sperm viability was higher.

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Effect of Season on Testis Function and Freezing and Fertilizing Ability of Spermatozoa in Korean Native Goat II. Seasonal and Diurnal Change in Serum Testosterone Level (한국재래산양에서의 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 II. 혈청내 Testosterone수준의 계절 및 하루중 변화)

  • Kim, C.K.;Chung, Y.C.;Kim, K.S.;Kim, S.;Kwon, C.J.;Han, K.Y.;Yoon, J.T.;Chung, Y.H.;Lee, J.H.
    • Korean Journal of Animal Reproduction
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    • v.17 no.4
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    • pp.325-329
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    • 1994
  • Seasonal and diurnal variation in serum testosterone was measured by radioimmunossary in 3 mature Korean native male goats. Blood was collected at 3-h intervals for 24h in each season. Mean levels of 4 seasons ranged from 0.82 to 2.06ng/ml and no difference was among seasons, although level in fall and winter was slightly higher. Diurnal level tended to increase at daytime in all season and when diurnal variation in level was compared to daily mean of each season, level of daytime greatly(P<0.05) increased in summer. However, variation between light and dark period in other seasons did not show significant difference. Daytime increase of testosterone was found 3 to 4 after dawn and level increased 1 to 2h ahead in summer and fall than that in others. These results in serum testosterone suggest that there was no great seasonal variation in endocrine function of goat testis.

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Testicular Cycles in the Korean Frogs: Annual Spermatogenic Patterns, Seasonal Changes in the Steroidogenic Competence, and Responsiveness Gonadotropins in vitro

  • Go, Seon-Gun;Gang, Hae-Muk;Kim, Jeong-U;Gwon, Hyeok-Bang
    • Animal cells and systems
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    • v.1 no.1
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    • pp.99-105
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    • 1997
  • Using three species of Korean frogs (Rana dybowskii, R. rugosa and R. nigromaculata), the annual spermatogenic pattern, the seasonal changes in the steroidogenic competence, and responsiveness of testis to gonadotropins in terms of testosterone secretion in vitro were examined. The spermatogenic pattern of R. dybowskii was classified as a discontinuous type since spermatogenesis stops completely after spawning in late winter (February) until mid-summer (July). In contrast, the pattern of R. nigromaculata and R. rugosa was classified as a potent continuous type since sperm was always present in the seminiferous tubules all year round. In all three species, the levels of testicular testosterone and that of testosterone secreted by testis following in vitro culture were very low in late summer (August), but increased thereafter until winter (hibernation period). Interestingly, responsiveness of testis in vitro to gonadotropins in terms of testosterone secretion increased markedly in November (early hibernation period). Specifically, bullfrog LH was more effective than FSH in stimulating the secretion of testosterone by frog testis in vitro during hibernation period. This fact suggests that testosterone secretion by testis during hibernation is at least regulated by the pituitary gonadotropin rather than environmental factors. Taken together, the data presented here suggest that testicular cycles of three species of Korean frogs are closely linked to their females breeding cycles, and are eventually controlled by various environmental cues.

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Some Chromosome Alteranations in the Cultured Chinese Hamster Cells Treated by Steroids (Steroid 물질처리를 받은 Chinese hamster 세포에 있어서의 염색체 이상)

  • 강영선
    • The Korean Journal of Zoology
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    • v.6 no.2
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    • pp.21-27
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    • 1963
  • This study is concerned with alterations in chromosomes (numbers and morphology) when the culture of Chinese hamster cells (FAF-28 strain) was treated by steroids, testosterone and DOC. 1. In 200 cells of normal untreated cells as control population the chromosome of stemline was decided as which was contained in 158 cells ; that is , in 79 percent of the population. The average chromosome number in above 20 cells observed was calculated as 23.95 with minimum limit at 20 and maximum limit at 70. 2. Many different chromosome numbers, ranging from 19 to 352 were observed in the 200 cells treated by testosterone. The diploid number of 22 showed the peak of variation curve was counted in 71 cells (35.5%) and an average chromosome number of stemline was 22 which was counted in 74 cells (37%). While all of the chromosome number of stemline was 22 which was counted in 74 cells (37%). While all of the chromosome numbers in the 200 cells observed ranged from 20 to 181 , an average chromosome number was also found to be 30.09. 4. The chromosome component in the cultured normal FAF-28 cells with 22 diploid chromosomeswas as follows ; 9a) 2 paris were long and metacentric (LM), (b) 3 pairs were medium length and metacentric (MM), (c) 3 pairs were small and subtelocentric (SS) and (d) 3 pairs were small and metacentric (SM). 5. The twenty cells with 44 chromosomes were selected at random from each cell population treated with testosterone and DOC , so that chromosome idiogram and morphology could be studies. In the twenty cells of the testosterone treated population the average ratio of above four groups, LM ; MM;Ss:SM, was found to be 8.6 : 10.8:13.5:10.7. On the other hand, the average ratio in the same number of cells of the DOC treated one was 7.7 :11.4:12.5:12.7. 6. The five types of the altered chromosomes morphologically in the hundred cells selected at random from each cell population treated by testosterone and DOC were observed (Type I-V). The thirty-one altered chromosomes were found to be in the testosterone treated cell population and the sixteen in DOC treated.

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TRPV1 activation induces cell death of TM3 mouse Leydig cells

  • Kim, Eun-Jin;Dang, Long Cao;Nyiramana, Marie Merci;Siregar, Adrian S.;Woo, Min-Seok;Kim, Chang-Woon;Kang, Dawon
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.3
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    • pp.145-153
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    • 2021
  • The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicin-induced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.

Inhibitory effects of abietic acid in testosterone-induced benign prostatic hyperplastic rats (송진 유래 abietic acid가 전립선 비대증 모델 rat에 미치는 영향)

  • So-Young, Kim;Yoo-Jin, Kim;Yong-ung, Kim;Mi Ryeo, Kim
    • The Korea Journal of Herbology
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    • v.38 no.2
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    • pp.27-34
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    • 2023
  • Objectives : Currently, the benign prostatic hyperplasia (BPH) is one of the most common urogenital disorder in old men. We were performed to determine the effects of abietic acid (AC), component of pine resin, in benign prostatic hyperplastic Sprague-Dawley rat (SD rat) induced by testosterone injection (IP). Methods : We monitored body weights in SD rat at start and end date of experiment. After end of experiment, the prostate weights were measured in SD rats. Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels was performed in serum. And we determined the 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels in prostate tissue and serum using ELISA kit. Results : As results, the prostate wights were increased in BPH group compared to normal group and were decreased in fina, AC30, and AC 50 groups, respectively. Serum GOT levels were decreased in AC50 group compared to BPH group. And Serum GPT levels of AC30 and AC50 groups were lower than BPH group. In addition, the 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels were decreased the fina, AC10, AC30, and AC 50 groups contrast to the BPH group. Furthermore, 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels were decreased dose dependent in AC groups compared to BPH group. Conclusion : These results suggest that AC could be used as a potential material for the treatment of BPH by decreasing the androgen levels in benign prostatic hyperplasia model rats.

Immersion in sea cucumber's steroid extract to increase male production of juvenile freshwater crayfish

  • Gregorius Nugroho Susanto;Endang Linirin Widiastuti;Tri Rustanti;Sutopo Hadi
    • Fisheries and Aquatic Sciences
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    • v.26 no.1
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    • pp.48-57
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    • 2023
  • One of the ways to increase the production for aquaculture is through the cultivation of monosexuals by ensuring genital reversal from which energy for reproduction is diverted towards growth. Masculinization has been identified as one of the most prominent techniques, where sex development was directed from female to male. This approach only altered the phenotype and not the genotype. The red claw crayfish (Cherax quadricarinatus) was a relatively new commercial commodity, and the males were known to grow faster than females. Hence, it was proposed to use monocultures comprising an all-male population to increase yield using steroid hormone, synthetic 17α-methyltestosterone. However, this technique generated residues that detrimentally affect human health, the environment, and cultivated organisms. Therefore, finding new safe natural steroid sources was essential, and one of which is exploring of natural hormones extracted from the viscera of sea cucumbers (Holothuria scabra Jaeger). This study focused on the determination of male formation and testosterone levels among juvenile crayfish, after immersing in sea cucumber steroid extract (SCSE). A completely random design with factorial was used with two variables, encompassing the varied doses (0, 2, 4 mg/L, 2 mg/L 17α-methyl testosterone as control group) and immersion times of 18 and 30 h. The result showed the dose-dependent ability of SCSE increase the male genital formation and promote the testosterone level of juvenile crayfish. In addition, the testosterone was influenced by dose and immersion duration time, with the highest level of testosterone observed in treatments of 4 mg/L SCSE with 30 h immersion was 0.248 ng/mL, while the male percentage was 77%. In conclusion, the combination of dose and immersion time significantly affected growth and testosterone levels.