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Gut microbiota profiling in aged dogs after feeding pet food contained Hericium erinaceus

  • Hyun-Woo, Cho;Soyoung, Choi;Kangmin, Seo;Ki Hyun, Kim;Jung-Hwan, Jeon;Chan Ho, Kim;Sejin, Lim;Sohee, Jeong;Ju Lan, Chun
    • Journal of Animal Science and Technology
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    • v.64 no.5
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    • pp.937-949
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    • 2022
  • Health concern of dogs is the most important issue for pet owners. People who have companied the dogs long-term provide the utmost cares for their well-being and healthy life. Recently, it was revealed that the population and types of gut microbiota affect the metabolism and immunity of the host. However, there is little information on the gut microbiome of dogs. Hericium erinaceus (H. erinaceus; HE) is one of the well-known medicinal mushrooms and has multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine, hericerin, erinacines, etc. Here we tested a pet food that contained H. erinaceus for improvement in the gut microbiota environment of aged dogs. A total of 18 dogs, each 11 years old, were utilized. For sixteen weeks, the dogs were fed with 0.4 g of H. erinaceus (HE-L), or 0.8 g (HE-H), or without H. erinaceus (CON) per body weight (kg) with daily diets (n = 6 per group). Taxonomic analysis was performed using metagenomics to investigate the difference in the gut microbiome. Resulting from principal coordinates analysis (PCoA) to confirm the distance difference between the groups, there was a significant difference between HE-H and CON due to weighted Unique fraction metric (Unifrac) distance (p = 0.047), but HE-L did not have a statistical difference compared to that of CON. Additionally, the result of Linear discriminate analysis of effect size (LEfSe) showed that phylum Bacteroidetes in HE-H and its order Bacteroidales increased, compared to that of CON, Additionally, phylum Firmicutes in HE-H, and its genera (Streptococcus, Tyzzerella) were reduced. Furthermore, at the family level, Campylobacteraceae and its genus Campylobacter in HE-H was decreased compared to that of CON. Summarily, our data demonstrated that the intake of H. erinaceus can regulate the gut microbial community in aged dogs, and an adequate supply of HE on pet diets would possibly improve immunity and anti-obesity on gut-microbiota in dogs.

Anti-skin-aging Effect of Mori Folium through decreased Advanced glycation end product (AGEs) (최종당화산물 억제를 통한 상엽(桑葉)의 항피부노화 효과)

  • Lee, AhReum;Kim, SooHyun;Kim, SuJi;Kim, KyeongJo;Kwon, Ojun;Choi, JoonYoung;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.32 no.5
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    • pp.7-12
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    • 2017
  • Objectives : Recently, numerous studies reported that excessive generation of advanced glycation end products (AGEs) stimulated expression of skin wrinkle related proteins. This study aimed to evaluate inhibits skin wrinkle formation effect of Mori Folium (MF) through decreased AGEs. Methods : To evaluate the skin wrinkle inhibition effect of MF, SD-rats were divided into three groups; normal rats (Nor), AGEs-induced rats (Con), AGEs-induce rats treated with MF at dose of 100mg/kg body weight (MF). To induced AGEs, streptozotocin (50mg/kg) was injected intraperitoneally, and after 3 days, 100mM methyl glyoxal was administered orally for 3 weeks. After the experiment, the animal's dorsal skin tissues and serum were separated and tested. Results : The oral administration of MF suppressed the AGEs level in serum. Also, the AGEs in skin tissues was significantly reduced through treatment of MF compared with control group. Moreover, the expressions of AGEs related proteins such as polyclonal anti-$N^e$-(carboxymethyl) lysine (CML), anti-$N^e$-(carboxyethyl)lysine (CEL), AGE receptors (RAGE) were reduced in MF group compared with the control group in kidney and skin tissues. The matrix metallo proteinase-1 (MMP-1) reduced by MF treatment with the result that collagen type 1 alpha 2 (COL1A2) was improved that reduced by accumulation of AGEs. Conclusion : The evidence of this study indicate that oral administration of MF reduces the levels of AGEs in serum, skin, and kidney tissues. In conclusion, MF inhibit skin wrinkle formation, suggesting the potential of anti-wrinkle material.

A Study on the Development of Intravenous Injection Management Application for EMR System Interworking (EMR 시스템 연동 정맥주사 관리 애플리케이션 개발에 대한 연구)

  • Jin-Hyoung, Jeong;Jae-Hyun, Jo;Seung-Hun, Kim;Won-yeop, Park;Sang-Sik, Lee
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.15 no.6
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    • pp.506-514
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    • 2022
  • This paper is about developing an intravenous injection management system that can provide nurses with information related to intravenous injection in real-time to compensate for possible instability factors during intravenous injection. The intravenous injection management system consists of an app-based user S/W and a web-based administrator S/W. User S/W is implemented to provide users with the ability to identify patients who need intravenous injection through smartphones, tablet PCs, and nursing PDAs, recognize information codes given to patients, and enter and share treatment contents and treatment items after intravenous injection. As a result of intravenous injection treatment uploaded through the user app, the manager S/W can check the records of intravenous injection treatment items, perform user management functions, emergency notification registration and management functions, and data upload functions. The implemented system has not yet been tested on the EMR system used in the actual hospital. Therefore, through further research, S/W will be optimized and actual environmental application tests will be conducted through cooperation with hospitals.

The Effects of Brand Attachment, Brand Name, and Brand Image Congruence on Brand Attitude, WOM and Revisit Intentions in the Restaurant Sector (브랜드 애착, 브랜드 네임, 브랜드 이미지 일치성이 태도, 구전 및 재방문의도에 미치는 영향)

  • KIM, Eun-Jung
    • The Korean Journal of Franchise Management
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    • v.13 no.2
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    • pp.53-66
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    • 2022
  • Purpose: How to build the attitude on brand is very important, because it affects the positive word of mouth and revisit intention. Brand attachment, brand name, and image congruence play important role on consumer behavior in terms of reinforcing consumers' perception of food service companies and differentiating them from competing brands. Following the planned behavior theory, this paper examines the effect of linking brand attitude to word-of-mouth and revisit intentions in the restaurant sector. Research design, data, and methodology: This paper examines the structural relationship among brand attachment, brand name, image congruence, brand attitude, WOM, and revisit intention. In order to test the purposes of this study, research model and hypotheses were developed. The questionnaire items were modified and used according to the content of this study based on previous studies. All constructs were measured by multiple items tested and developed in the previous research. The study is based on the quantitative method and considered 519 questionnaires fulfilled by customers of restaurants. The data were explored employing the partial least square-structural equation modelling (PLS-SEM). Frequency analysis was conducted to identify the general characteristics of the survey subjects. To measure the reliability and validity of the measurement tools, confirmatory factor analysis was conducted. Structural model analysis was conducted to verify the research model. Result: The findings demonstrate that brand attachment and brand name had positive effects on attitude while image congruence did not have. Also, attitude had positive effect on WOM and revisit intention. Conclusions: This study expands the literature about WOM and revisit intentions. This study expands prior research in a similar field to which the theory of planned behavior (TPB) is applied, and reveals that brand attachment, brand name, and brand image congruence play an important role in developing brand attitude that affect revisit intention and WOM. And provide guidelines on how to enhance competitiveness in the restaurant sector based on understanding of linking brand attitude to customer loyalty and repeat business. By putting into practice these suggestions in the restaurant industry, brands can easily build up their attitude and boost a positive WOM and the intention to revisit.

Effect on blood heavy metal concentration in gas poisoning by combustion of ignition coal: Pilot study (착화탄 연소에 의한 가스 중독 환자에서 혈중 중금속 농도의 영향에 대한 예비연구)

  • Lee, Sang Hwan;Lee, Juncheol;Cho, Yongil;Ko, Byuk Sung;Oh, Jaehoon;Kang, Hyunggoo
    • Journal of The Korean Society of Clinical Toxicology
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    • v.19 no.2
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    • pp.127-132
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    • 2021
  • Purpose: It is known that the most common cause of gas poisoning in Korea is suicide attempts by burning ignition coals. Ignition coals are made from waste wood, and studies have been reported that heavy metals are emitted when this coal is burned. However, there was no study on how much heavy metal poisoning occurs in the human body through this, so this study was planned to find out whether the concentration of heavy metals in the blood increased in patients exposed to ignition coal combustion. Methods: From April 2020 to April 2021, blood lead, mercury, and cadmium concentrations were investigated in carbon monoxide poisoning patients who visited one regional emergency medical center in Seoul, and their association with exposure time, source of poisoning, and rhabdomyolysis were investigated. Results: During the study period, a total of 136 carbon monoxide poisoning patients were tested for heavy metals, and 81 cases of poisoning by ignition coal were reported. When comparing poisoning caused by combustion of ignition coal and other substances, there was no difference in the concentrations of lead, mercury, and cadmium in the blood, and there was no difference in the number of patients above the reference range. However, the patients exposed to more than 5 hours of ignition coal gas exposure are more frequent than those in the group less than 5 hours in lead (51.4% vs. 23.9%, p=0.012). Conclusion: Compared to poisoning with other combustible substances, the blood concentration of lead, mercury, and cadmium does not increase further in patients with gas poisoning by ignition coal. However, prolonged exposure may result in elevated levels of lead.

Semantic Segmentation for Multiple Concrete Damage Based on Hierarchical Learning (계층적 학습 기반 다중 콘크리트 손상에 대한 의미론적 분할)

  • Shim, Seungbo;Min, Jiyoung
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.6
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    • pp.175-181
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    • 2022
  • The condition of infrastructure deteriorates as the service life increases. Since most infrastructure in South Korea were intensively built during the period of economic growth, the proportion of outdated infrastructure is rapidly increasing now. Aging of such infrastructure can lead to safety accidents and even human casualties. To prevent these issues in advance, periodic and accurate inspection is essential. For this reason, the need for research to detect various types of damage using computer vision and deep learning is increasingly required in the field of remotely controlled or autonomous inspection. To this end, this study proposed a neural network structure that can detect concrete damage by classifying it into three types. In particular, the proposed neural network can detect them more accurately through a hierarchical learning technique. This neural network was trained with 2,026 damage images and tested with 508 damage images. As a result, we completed an algorithm with average mean intersection over union of 67.04% and F1 score of 52.65%. It is expected that the proposed damage detection algorithm could apply to accurate facility condition diagnosis in the near future.

Synthesis of Linear 1,2-Alkylaminopropanediols as Preservatives and Antimicrobial Activity (보존제로서 선형 1,2-알킬아미노프로판디올의 합성과 항균활성)

  • Cha, Kyung-On;Kwak, Sang-Woon;Jeong, Kook-In;Kim, Young-Ho
    • Applied Chemistry for Engineering
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    • v.33 no.4
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    • pp.394-401
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    • 2022
  • The synthesis of 1,2-alkylaminopropanediols (1,2-AAPs) was designed to improve the hydrophilicity of linear 1,2-alkanediols having 10, 12, 14, and 16 carbon atoms in the alkyl chain. 1,2-AAPs were synthesized by reacting 3-monochloro-1,2-propanediol (3-MCPD) with linear alkylamines having 10, 12, 14 or 16 carbon atoms in an ethanol solvent at 40℃ for 2 h. The yield and purity of four types of 1,2-AAPs synthesized were found to be in the range of 51-58% and 85-99%, respectively. The amine salts of four types of 1,2-AAPs were prepared from a purified paste or solid compound by adding an acidic solution (HCl) to pH 7, and then their solubilities and antibacterial effects were tested. 1,2-decylaminopropanediol, 1,2-dodecylaminopropanediol, and 1,2-tetradecylaminopropanediol were all dissolved in water at concentrations of 100%, 50%, and 0.1%, respectively, however 1,2-hexadecylaminopropanediol was not. The antibacterial effect was improved as the length of the alkyl chain increased. As a result of confirming the preservative effect of the lotion (cosmetic formulation) applied with 1,2-AAP for application, it showed very strong antibacterial activity at low concentrations ranging from 0.005% to 0.2%.

A Study on UX of Shared Electric Scooters Using Gamification: Focusing on User Engagement and Motivation (게이미피케이션을 이용한 공유 전동킥보드 서비스 UX 연구: 사용자 참여와 동기 부여 향상을 중심으로)

  • Lee, Ja-Eun;Kim, Dongwhan
    • The Journal of the Korea Contents Association
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    • v.22 no.2
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    • pp.173-186
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    • 2022
  • The purpose of this study is to investigate the influence of gamification strategies on improving participation and motivation of shared electric scooter users. To this end, this study derived the user type through the first research question of how the shared electric scooter usage behavior and pulse are, and derived user tasks and scenarios. The second research question, a shared electric kickboard app with gamification, was tested by users to see if it helps increase user participation and form motivation. As a result of the analysis, it was found that users were induced to be considerate of other users by using a combination of the motivational, relational, and self-expression strategies of gamification. Second, it was found that the use of motivation, achievement and reward, and reward visualization strategy elements promotes user's voluntary behavior. Third, through relationship, achievement, and reward strategies, users participated to create a positive culture of shared electric scooters, drawing immediate feedback, indicating that convenience has increased. In conclusion, it was found that the user helped to play a positive role in voluntary participation and motivation through the use of the shared electric kickboard service with gamification.

Factors Affecting Elderly People's Intention to Use of Digital Wealth Management Services (고령자들의 디지털 자산관리 서비스 이용의도에 영향을 미치는 특성 및 요인)

  • Kwak, Jae-Hyuk;Dong, Hak-Lim
    • Journal of Digital Convergence
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    • v.20 no.5
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    • pp.411-422
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    • 2022
  • The purpose of this study was to identify factors that affect the characteristics and intentions of the elderly to use digital wealth management services. The subjects of this study were 312 elderly people over 50 years old. Based on the Value-based Adoption Model(VAM), the research model added price value, social influence, and perceived risk as research variables. As a result of empirical analysis, it was found that usefulness, enjoyment, price value, and social influence all had a significant positive (+) effect on perceived value. It was found that technicality had a significant negative (-) effect. On the other hand, no significant effect relationship was tested on perceived risk. The perceived value had a significant positive (+) effect on the intention to use. This study was meaningful in the academic research that it applied a research model that reflected the characteristics of the elderly who were not treated as mainstream in the technology acceptance model for digital wealth management services. In addition, it provided practical implications for providers' marketing strategies and government/public institution policy establishment to increase the use of digital wealth management services for the elderly.

Impact of Direct Structured Instruction for Students with Learning Disabilities on Engineering Physics Concepts (공대 물리학 교육에서 학습장애자에 대한 직접교수법의 효과)

  • Hwang, Un-Hak
    • Journal of Practical Engineering Education
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    • v.14 no.1
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    • pp.19-25
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    • 2022
  • This study examined the impact of direct structured approach of students who demonstrate little or no sense of basic engineer concepts in physics courses. This direct structured instruction is one of the methodologies that focuses on explicit and systematic practices in which an instructor set clear learning outcomes and clarifies the direction of the instruction. 90 participants were randomly selected and tested on the areas of problem-solving skills, reasoning, working memory, and processing speed. 20% of the participants were found to be students with basic engineering disabilities. On the other hand, in the direct structured group, 51.7% and 58.0% of the sample group (90 students) showed a 6.3% increase from the mid-term to final examinations, respectively. The subgroups with 50% or lower grades were decreased from 26.7% to 24.5%. However, five students with the lowest grade of 20% were selected as students with learning disabilities in the study and the average scores of mid-term and final exams were increased by 8.6%, which was 17.9% and 26.5%, respectively at the end of the study. As a result, it showed that direct structured approach for students with learning disabilities in the engineer concepts was effective.