• The Journal of the Convergence on Culture Technology
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• v.5 no.1
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• pp.413-416
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• 2019

Alstroemeria (Alstroemeriaceae) is one of the most important cut flowers in international market. Especially, characteristics like long vase-life, various colors, tolerance to low temperature and a low energy requirement during cultivation have stimulated this success. Because of its characteristics such as low multiplication rates, time-consuming process and high risk of carrying viral disease, in vitro propagation techniques based on rhizome meristems culture have been developing nowadays. The callus induction has various cultivation sites compared with the direct plant generation method, and if the callus is maintained well, the plant differentiation can be performed simultaneously while maintaining the callus, so that it can be used for mass proliferation. In this study, we tested various hormones and cultivars for efficient callus induction. As a result of culturing between the nodes and the internodes, the callus began to be formed after 8 weeks, and the calli incidence in the nodes was higher than that between the internodes. Also, in the comparison of 2,4-D and picloram, the callus incidence rate was up to 2 times higher in the medium treated with 2,4-D. Using these results, it is thought that it will help establish the system of mass propagation system of Alstroemeria and cultivate new varieties.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.5
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• pp.322-333
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• 2019

The purpose of the current study was to investigate the level of market size and market share of domestic research equipments for analyzing the difference of the number and amount of construction by the manufacturing countries in the basic and analytical science fields based on the information of the research equipment invested by the Korean government for the past 14 years. As of January 1 2019, from 2005 to 2018, 20,687 research facilities & equipments (main equipment with a construction cost of 30 million won or more) built in the basic and analytical science fields were selected for this study and their components, standard classification, number of construction, and amount of construction by country of manufacture were analyzed. Differences of the number and amount of construction among manufacturing countries were tested using a single sample chi-square test and one-way analysis of variance, followed by Bonferroni's post-hoc test. As a result of this study, the number of construction (p<.001) and construction amount (p<.05) were statistically different for each manufacturing country. The level of market size and market share was significantly different according to the equipment standard classification (p<.05). Therefore, differentiated strategies of the government and policy research projects will be required for each type of equipment and amount in order to support the policy for the localization of research equipment.

• Korean Journal of Environmental Biology
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• v.39 no.3
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• pp.319-328
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• 2021

Tomato spotted wilt virus (TSWV) is one of the most destructive viruses worldwide, which causes severe damage to economically important crops, such as pepper and tomato. In this study, we examined the molecular and biological characterization of a TSWV isolate (SW-TO2) infecting tomato and compared it to the recently reported isolates from boxthorn, butterbur, and angelica plants. The phylogenetic analysis based on the complete genome sequences confirmed that SW-TO2 was clustered with those of isolates from boxthorn and pepper in Korea with the maximum nucleotide identities ranging from 98% to 99%. We developed the bioassay method for screening TSWV resistance and tested some commercial pepper and tomato cultivars for resistance evaluation of four isolates of TSWV. TSWV resistance was evaluated as TSWV resistance when all the following three conditions were satisfied: first, when symptoms of necrotic spots or no symptoms were present in the inoculated leaves; second, when there were no symptoms in the upper leaves; and third, when the upper leaves were negative as a result of RT-PCR diagnosis.

• Journal of Ecology and Environment
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• v.45 no.3
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• pp.143-151
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• 2021

Background: Plants are able to optimize defense responses induced by various herbivores, which have different feeding strategies. Local and systemic responses within a plant after herbivory are essential to modulate herbivore-specific plant responses. For instance, leaf-chewing herbivores elicit jasmonic acid signaling, which result in the inductions of toxic chemicals in the attacked leaf (tissue-specific responses) and also in the other unattacked parts of the plant (systemic responses). Root herbivory induces toxic metabolites in the attacked root and alters the levels of transcripts and metabolites in the unattacked shoot. However, we have little knowledge of the local and systemic responses against stem-boring herbivores. In this study, we examined the systemic changes in metabolites in the wild tobacco Nicotiana attenuata, when the stem-boring herbivore Trichobaris mucorea attacks. Results: To investigate the systemic responses of T. mucorea attacks, we measured the levels of jasmonic acid (JA), JA-dependent secondary metabolites, soluble sugars, and free amino acids in 7 distinct tissues of N. attenuata: leaf lamina with epidermis (LLE), leaf midrib (LM), stem epidermis (SE), stem pith (SP), stem vascular bundle (SV), root cortex with epidermis (RCE), and root vascular bundle (RV). The levels of JA were increased in all root tissues and in LM by T. mucorea attacks. The levels of chlorogenic acids (CGAs) and nicotine were increased in all stem tissues by T. mucorea. However, CGA was systematically induced in LM, and nicotine was systematically induced in LM and RCE. We further tested the resource allocation by measuring soluble sugars and free amino acids in plant tissues. T. mucorea attacks increased the level of free amino acids in all tissues except in LLE. The levels of soluble sugars were significantly decreased in SE and SP, but increased in RV. Conclusions: The results reveal that plants have local- and systemic-specific responses in response to attack from a stem-boring herbivore. Interestingly, the level of induced secondary metabolites was not consistent with the systemic inductions of JA. Spatiotemporal resolution of plant defense responses against stem herbivory will be required to understand how a plant copes with attack from herbivores from different feeding guilds.

• Journal of Internet Computing and Services
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• v.22 no.1
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• pp.23-30
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• 2021

This paper proposes a noise-tolerant image classification system using multiple autoencoders. The development of deep learning technology has dramatically improved the performance of image classifiers. However, if the images are contaminated by noise, the performance degrades rapidly. Noise added to the image is inevitably generated in the process of obtaining and transmitting the image. Therefore, in order to use the classifier in a real environment, we have to deal with the noise. On the other hand, the autoencoder is an artificial neural network model that is trained to have similar input and output values. If the input data is similar to the training data, the error between the input data and output data of the autoencoder will be small. However, if the input data is not similar to the training data, the error will be large. The proposed system uses the relationship between the input data and the output data of the autoencoder, and it has two phases to classify the images. In the first phase, the classes with the highest likelihood of classification are selected and subject to the procedure again in the second phase. For the performance analysis of the proposed system, classification accuracy was tested on a Gaussian noise-contaminated MNIST dataset. As a result of the experiment, it was confirmed that the proposed system in the noisy environment has higher accuracy than the CNN-based classification technique.

• Journal of Digital Convergence
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• v.19 no.8
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• pp.21-32
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• 2021

The government is focusing its attention and support on start-ups. Nevertheless, there is anxiety and fear about starting a business at the base of public awareness. Experienced as a way to overcome fear and difficulty. Few prior studies have been done on experience factors as ones influencing entrepreneurial intention. In this study, I studied whether the experience of successful entrepreneurship through cultural indirect experience affect the resolution of fear about establishing a business. Among the influencing factors on the entrepreneurial intention, business motivation, social support and awareness of entrepreneur were selected as independent variables. In addition, by applying the cultivation theory, the drama role models were set as a controlling variable. For empirical analysis, a survey was conducted targeting 399 ordinary persons. The hypothesis was tested through regression analysis using the SPSS 23 statistical package. The moderating effect was analyzed using Process Macro 3.5. Self-fulfillment, livelihood, economic motivation, social support, and awareness of entrepreneur are sub-factors of business motivation, And all of these had a positive significant effect on entrepreneurial intention. Among the significant variables, self-fulfillment was found to have the greatest effect. On the other hand, as a result of analyzing the moderating effect of the drama role model, it was found play a role in controlling between self-fulfillment and entrepreneurial intention, between livelihood and entrepreneurial intention, and between awareness of entrepreneur and entrepreneurial intention. Based on these research results, academic and practical implications were presented.

• Journal of Digital Convergence
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• v.17 no.11
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• pp.171-181
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• 2019

In order to confirm whether innovation can be realized through the direction that companies pursue, this study empirically analyzed the relationship between strategic directionality and innovation capacity. Specifically, strategic directionality is divided into customer orientation and technology orientation, and innovation capacity is classified into strategic planning capacity, R&D capacity, and technology commercialization capacity based on previous studies. And the effect of customer and technology orientation on each innovation capacity was analyzed. Data collection was carried out by 368 questionnaires from the workers of manufacturing industry in Gyeongbuk area and the proposed hypotheses were tested by using structural equation. As a result, it was confirmed that technology orientation had significant effects on all three innovation capacities. However, the effect of customer orientation on strategic planning capacity and technical commercialization capacity was accepted, but the relationship with R&D capacity was rejected. These results can provide implications for how to draw innovation from the organizational strategic direction and culture perspectives by explaining the impact of organizational orientation on innovation capacity.

• Journal of Life Science
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• v.31 no.2
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• pp.164-174
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• 2021

This study confirmed the potential of Punica granatum L. extract for functional activity verification and cosmetic development. The electron-donating ability of Punica granatum L. extract was shown 60.6% at a 1,000 ㎍/ml concentration. Its ABTS+ radical scavenging ability was shown 93.9% at a 1,000 ㎍/ml concentration. Additionally, the inhibitive effects of elastase and collagenase inhibition effects were measured as 30% and 47.2%, respectively, at a 1,000 ㎍/ml concentration. To determine the effect of Punica granatum L. extract on the proliferation of fibroblasts (CCD-986sk), cell viability was measured using a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. As a result, survival rates of 130% or higher at a 500 ㎍/ml concentration or less were confirmed. According to the results of Western blot with Punica granatum L. extract, the expression inhibition rates of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-3 (MMP-3) were decreased by 23.2%, 81.9%, and 69.2%, respectively, at a 100 ㎍/ml concentration. Based on the results above, O/W liquid crystal cream with 0.1% Punica granatum L. extract was prepared. The stabilities were tested at 4, 25, 45, and 50℃. By checking the pH, change over time, and stability by temperature, it was confirmed that all were stable for one month. Thus, Punica granatum L. extract shows potential as a natural material for cosmetics.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.35 no.6
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• pp.111-119
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• 2019

The purpose of this study is to investigate the shear strength of reinforced concrete beam according to beam section size and shear reinforcement ratio. A total of nine specimens were tested and designed concrete compressive strength is 24 MPa. The main variables are shear reinforcement ratio and beam section size fixed with shear span to depth ratio (a/d = 2.5), the tensile reinforcement ratio (${\rho}=0.013$) and width to depth ratio (h/b = 1.5). The test specimens were divided into three series of S1 ($225{\times}338mm$), S2 ($270{\times}405mm$) and S3 ($315{\times}473mm$), respectively. The experimental results show that all specimens represent diagonal tensile failure. For $S^*-1$ specimens (d/s=0), the shear strength decreased by 33% and 46% with increasing the beam effective depth, 26% and 33% for $S^*-2$ specimens (d/s=1.5) and 16% and 20% for $S^*-3$ specimens (d/s=2.0) respectively. As the shear reinforcement ratio increases, the decrease range in shear strength decreases. In other words, this means that as the shear reinforcement ratio increases, the size effect of concrete decreases. In the S1 series, the shear strength increased by 39% and 41% as the shear reinforcement ratio increased, 54% and 76% in the S2 series and 66% and 100% in the S3 series, respectively. As the effective depth of beam increases, the increase range of shear strength increases. This means that the effect of shear reinforcement increases as the beam effective depth increases. As a result of comparing experimental values with theoretical values by standard equation and proposed equation, the ratio by Zsutty and Bazant's equation is 1.30 ~ 1.36 and the ratio by KBC1 and KBC2 is 1.55~.163, respectively. Therefore, Zsutty and Bazant's proposed equation is more likely to reflect the experimental data. The current standard for shear reinforcement ratio (i.e., $S_{max}=d/2$) is expected to be somewhat relaxed because the ratio of experimental values to theoretical values was found to be 1.01 ~ 1.44 for most specimens.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.611-619
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• 2021

Porous asphalt pavement (PAP) has many functions, such as reducing accidents and decreasing noise. On the other hand, vulnerability is inevitable because PAP contains approximately 20% porosity. This study evaluated the effects of the maximum aggregate size (MAS), temperature, and porosity on the PAP durability. The indirect tensile strength measures durability. This study tested the samples that stayed dry and were moisturized by freezing and thawing for mixtures having the same porosity of 20% and MAS of 13mm, 10mm, and 8mm. The same test was performed on a mixture of 20% and 22% voids made of the same material with a MAS of 10mm. As a result, for 20% porosity, significant differences in the changes in MAS and temperature were found. A clear difference was observed between 8mm and 13mm under dry conditions, but there were no other significant differences in the MAS change. Furthermore, there was a clear difference in temperature for the change in porosity and temperature, but the gap in 2% porosity at 20% did not show a clear difference. Therefore, it is necessary to develop a more durable PAP through quantitative evaluations of the factors affecting the PAP durability.