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Processing of Radish Juice by Mixed Culture with lactic Acid Bacteria (유산균의 혼합배양에 의한 무주스의 가공)

  • 김정희;김종일
    • Food Science and Preservation
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    • v.6 no.4
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    • pp.448-455
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    • 1999
  • The starters, 0.3% of Leuconostoc mesenteroides, Lactobacillus brevis and Lactobacillus fermentum, were added in radish juice to process the radish juice by single and mixed cultures. The radish Juice was fermented for 7 days at 25$^{\circ}C$, 20 days at 15$^{\circ}C$, and 36 days at 5$^{\circ}C$. When fermented at 25$^{\circ}C$, the number of lactic acid bacteria in the juice made with mixed culture was higher than that of the single culture. But, juice fermented at low temperatures (15∼5$^{\circ}C$), the addition of starters was not effective, although there were some differences by inoculation strains. Although there was a little differences by inoculation strains, the content of nonvolatile organic acid and L-ascorbic acid were found more in the juice inoculated with lactic acid bacteria than the juice not inoculated. When the single and mixed cultures at the optimal maturity were tested, the significant difference was found at 5% level except the yeasty and moldy smell and the unripe taste. According to the preference test, the mixed-cultured radish juice incubated at 25$^{\circ}C$with Lactobacillus brevis and Leuconostoc mesenteroides were evaluated superior to commercial Dongchimi. As a result, taste and quality of radish juice was improved by addition of starters.

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A Study on the Development of Soil-based PTMs for Analysis of Benzo[a]pyrene - Focusing on the Evaluation of Homogeneity and Stability for the Certification of Benzo[a]pyrenecandidate Reference Materials - (Benzo[a]pyrene 분석용 토양 숙련도 표준시료 개발에 관한 연구 - 후보 표준물질의 인증을 위한 균질성, 안정성 평가를 중심으로 -)

  • Lee, Minhyo;Lee, Guntaek;Joo, Changkyu;Kim, Yonghun;Lee, Bupyoel;Choe, Sunghun;Kim, Myeongock;Hong, Sukyoung;Kim, Gumhee;Lee, Wonseok
    • Journal of Soil and Groundwater Environment
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    • v.19 no.6
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    • pp.49-58
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    • 2014
  • This study was implemented as a part of the experiment to develop two kinds of soil-based Benzo[a]pyrene (BaP) proficiency testing materials (PTMs) for soil analysis. A test was carried out for the check of solubility of the reference material (high purity reagent) using several solvents. Another test was also conducted for the evaluation of homogeneity and stability of two kinds of candidate soil reference materials. The test analysis of BaP in terms of the candidate materials was conducted according to the Standard Soil Analytical Methods by Ministry of Environment. Dissolution of the reference material was shown to vary depending on solvent type and was higher in the order of Dichloromethane > Acetone > Acetone/MeOH (9 : 1) > N-hexane. In addition, the slope on calibration curve for BaP standard solutions was largest on BaP standard solutions prepared with dichloromethane of the tested solvents. Such tendency appeared egually in the commercial BaP standard solution. Therefore, it is thought to be reasonable to use dichloromethane as the solvent in case of the standard stock solution that is used for the measurement of BaP concentration in soil. ISO 13528 and IUPAC protocol were used for verification of homogeneity on the two kinds of soil candidate materials, Both candidate materials were sufficiently homogeneous. Stability assessment of the two candidate materials was made according to ISO Guide 35 and the result showed that both batches did not have any long-term and short term stability issues that might occur during shipping. However, monitoring results of BaP concentration in soil showed that BaP concentration of the two batches measured at 15 days after the sample preparation was reduced by about 24~37% compared with that of the samples measured on 0 day of the sample preparation. Identification was done with several treatments such as irradiation and sterilization etc. The major cause was shown to be irradiation to the samples.

The Study on Blast Effects of Stemming Materials by Trauzl Lead Block Test and High Speed 3D-DIC Systems (트라우즐 연주시험 및 고속 3차원 이미지영상상관 기법을 이용한 전색재 별 발파효과에 대한 연구)

  • Ko, Younghun;Seo, Seunghwan;Kim, Sik;Chung, Youngjun;Chung, Moonkyung
    • Journal of the Korean Geotechnical Society
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    • v.37 no.10
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    • pp.13-25
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    • 2021
  • The most widely used method for determining the blast effects of explosives is the Trauzl test. This test is used to measure the explosive power (strength) of a substance by determining volume increase, which is produced by the detonation of a tested explosive charge in the cavity of a lead block with defined quality and size. In this paper, Trauzl lead block test and High speed 3D-DIC (Digital Image Correlation) system were conducted to evaluate the stemming effect of the blast hole. The effects of stemming materials can be expressed as the expansion of the cavity in a standard lead block through explosion of the explosives. The blasting experiment was conducted with emulsion explosives. The stemming material in the blast hole of lead block, which was adopted in this study, were using sand and stone chips. Results of blasting experiment and numerical analysis showed that the expansion rates of lead block were most affected by stone chips followed by sand. Also, as result of dynamic strain measurement on the lead block surface of High speed 3D-DIC system, the displacement and surface strain on the block were the highest in the experiment case of stone chips stemming.

Electrical Resistivity of ITZ According to the Type of Aggregate (골재 종류별 시멘트 경화체 계면의 전기저항 특성)

  • Kim, Ho-Jin;Bae, Je Hyun;Jung, Young-Hoon;Park, Sun-Gyu
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.3
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    • pp.268-275
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    • 2021
  • The three factors that determine the strength of concrete are the strength of cement paste, aggregate and ITZ(Interfacial Transition Zone) between aggregate and cement paste. Out of these, the strength of ITZ is the most vulnerable. ITZ is formed in 10~50㎛, the ratio of calcium hydroxide is high, and CSH appears low ratio. A high calcium hydroxide ratio causes a decrease in the bond strength of ITZ. ITZ is due to further weak area. The problem of ITZ appears as a more disadvantageous factor when it used lightweight aggregate. The previous study of ITZ properties have measured interfacial toughness, identified influencing factors ITZ, and it progressed SEM and XRD analysis on cement matrix without using coarse aggregates. also it was identified microstructure using EMPA-BSE equipment. However, in previous studies, it is difficult to understand the microstructure and mechanical properties. Therefore, in this study, a method of measuring electrical resistance using EIS(Electrochemical Impedance Spectroscopy) measuring equipment was adopted to identify the ITZ between natural aggregate and lightweight aggregate, and it was tested the change of ITZ by surface coating of lightweight aggregate with ground granulated blast furnace slag. As a result, the compressive strength of natural aggregate and lightweight aggregate appear high strength of natural aggregate with high density, surface coating lightweight aggregate appear strength higher than natural aggregate. The electrical resistivity of ITZ according to the aggregate appeared difference.

Plant Growth-Promoting Activity Characteristics of Bacillus Strains in the Rhizosphere (근권에 존재하는 Bacillus 속 균주들의 식물 생장 촉진 활성 특성)

  • Oh, Ka-Yoon;Kim, Ji-Youn;Lee, Song Min;Kim, Hee Sook;Lee, Kwang Hui;Lee, Sang-Hyeon;Jang, Jeong Su
    • Microbiology and Biotechnology Letters
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    • v.49 no.3
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    • pp.403-412
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    • 2021
  • This study aimed to identify plant growth-promoting activity, phytopathogenic fungi growth inhibitory activity, mineral solubilization ability, and extracellular enzyme activity of the genus Bacillus in soil and the rhizosphere. With regards to antifungal activity against phytopathogenic fungi, DDP257 showed antifungal activity against all 10 pathogenic fungi tested. ANG20 showed the highest ability to produce indole-3-acetic acid, a plant growth-promoting factor (70.97 ㎍/ml). In addition, 10 species were identified to have 1-aminocyclopropane-1-carboxylate deaminase production ability, and most isolates showed nitrogen fixation and siderophore production abilities. Thereafter, the isolated strains' ability to solubilize minerals such as phosphate, calcite, and zinc was identified. With extracellular enzyme activity, the activity appeared in most enzymes. In particular, all the strains showed similar abilities for alkaline phosphatase, esterase (C4), acid phosphatase, and naphtol-AS-BI-phosphohydrolase production. This result was observed because the genus Bacillus secreted various organic substances, antibiotics, and extracellular enzymes. Therefore, through the results of this study, we suggest the possibility of using strains contributing to the improvement of the soil environment as microbial agents.

The Effect of STEAM Camp Program for Gifted High School Students on Their Creative Leader Competency and STEAM Literacy (STEAM 캠프를 통한 영재학급 고등학생의 융합인재소양과 창의적 인재 역량 변화)

  • Kim, Hak Bum;Cha, Jeongho
    • Journal of Science Education
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    • v.45 no.2
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    • pp.231-246
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    • 2021
  • The Korean Ministry of Education has emphasized the convergent ability for human resource cultivating the development of science and technology in the future. Based on this, Korean STEAM education aims to enhance students' interest and their understanding of science and technology as well as to develop students' convergence thinking and problem-solving skills. Through the camp for gifted students, students are generally introduced to produce self-directed outputs in order to solve the problem in everyday life. In this study, the author aims to find out whether the gifted high school students' creative leader competency and STEAM literacy changed after they experienced STEAM-based camp. 517 gifted students from 52 high schools in the Metropolitan city region join the camp, which is focused on generating student-centered outputs for problem-solving in daily life context. Before and after the camp, the creative leader competency and the STEAM literacy of participants were tested and compared. From the result, we found that the gifted high school students' creative leader competency and STEAM literacy improved after participating in the STEAM camp. In particular, all the sub-factors except for the social value pursuit domains in the social characteristic area of creative leader competency and the areas of creativity, respect, and communication in STEAM literacy increased. Educational implications will be discussed.

Effect of Illuminance on Color-based Analysis of Diabetes-Related Urine Fusion Analytes on Dipstick Using a Smartphone Camera (스마트폰 카메라를 활용한 뇨시험지 당뇨병관련 융합 분석인자의 색기반 분석에 미치는 외부 조도 영향)

  • Kim, Na-Kyung;Cho, Young-Sik;Kim, Seon-Chil
    • Journal of the Korea Convergence Society
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    • v.12 no.5
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    • pp.93-99
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    • 2021
  • Recently, the miniaturization and digitalization for the inspection devices of point-of-care testing (POCT) are rapidly evolving. In the urine test, a lot of researches on index paper technology are being conducted because people can be self-diagnosed through visual color comparison using a urine test paper, Dipsick. The purpose of this study is to analyze the RGB values from the color changes on Dipstick Pad, which isused for urine test, using a smartphone camera. To this end, the primary, analytes in urine wasdiabetes-related parameters such as glucose, ketone body and pH, which is the most frequently tested elements, and we pursuited to quantify the changes in dipstick color caused from artificial urine containing different ranges of sugar, ketone body, and pH. In this experiment, changes in RGB values under bright and dark illuminances were compared, and changes in RGB value were monitored as a function of concentration of analytes under the ambient illumination of laboratory. As a result, color separation at the bright luminance region was good, but it did not appearat the low luminance region, and the changed profiles in RGB value under different illuminances was suggested to correct the problem of the color separation algorithm.

Validity and Reliability of Daily Life Stress Scale for College Students (대학생 일상생활 스트레스 측정 도구의 타당도와 신뢰도)

  • Park, Jeong-Hye;Kang, Se-Won
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.5
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    • pp.423-432
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    • 2021
  • This study developed a scale to measure college students' perceived stress levels for adverse life events that may occur in their daily lives and confirmed its validity and reliability. The scale was developed in accordance with DeVellis' scale development guidelines. Data were collected from 1,242 students of a local university in 2020. The collected samples were randomly separated into two groups (A, B). Group A (N=620) was tested for an initial examination of the performance, exploratory factor analysis, multitrait-multimethod matrix, criterion-related validity, and reliability of each item; and verified with group B (N=622) for confirmatory factor analysis and reliability re-test. As a result, the final scale of 33 items and eight factors were developed. The KMO values were 0.92, and Bartlett's spherical test was significant (χ2=12532.42, p<.001); the number of factors with initial eigenvalues of 1.0 or higher was eight; the cumulative factor loadings of 71.5% and the commonality of each item was 0.56 or higher. The reliability of the scale was Cronbach's alpha 0.94; sub-factors' Cronbach's alpha was 0.78 to 0.90. Therefore, these findings suggest that the scale developed in this study would be useful for measuring the stress levels of daily life for college students.

Convergence study of blue light transmittance comparison between tips for light curing machine and commercially available blue light blocking lenses and yellow tinted lenses (광중합기용 팁과 시판용 청광 차단 렌즈 및 노란색 착색 렌즈의 청광 투과율 비교 융합 연구)

  • Lee, Sook-Jeong
    • Journal of the Korea Convergence Society
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    • v.12 no.7
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    • pp.125-131
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    • 2021
  • Blue light with strong energy is required for light-curing resin treatment, which is being used more frequently in dentistry. To reduce the risk of exposure to scattered light, we tried to use colored lenses. The tips for light curing machine and a commercially available yellow-type blue-light blocking lens and a yellow lens colored with yellow dye, which are expected to be effective in blocking blue light, were placed in a UV-Vis spectrometer device, and transmission and blocking of blue light were tested respectively. As a result, the average blue light blocking rate of the light curing machine tips was 99.49%, and the C lens with the highest color density among commercially available lenses showed a high blue light blocking rate of 99.54%. In the case of lenses tinted with yellow, the yellow tinted C lens with the highest tint concentration showed 87.57% of blue light blocking rate. It is judged that the side effects related to the eyes caused by blue light can be reduced if a yellow-type commercially available or colored lens is worn along with a light curing machine tip during resin treatment.

A Study on the Condition Analysis and Improvement of Domestic Medical 99Mo/99mTc Generators Self-disposal (국내 의료용 99Mo/99mTc Generator 자체 처분 지침 현황 분석 및 개선 방향에 대한 연구)

  • Ryu, Chan-Ju;Hong, Seong-Jong
    • Journal of the Korean Society of Radiology
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    • v.13 no.2
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    • pp.297-303
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    • 2019
  • The nuclear medicine department of a domestic medical institution uses $^{99m}TcI$, a radionuclide, from $^{99}Mo/^{99m}TcI$ Generator, to inject radioactive drugs into patients. Among the expired generators, imported from foreign countries, the medical institution implements its own disposal. Each medical institution shall satisfy the permitted in-house disposal concentration of radioactive wastes. The guidelines for self-disposal presented in Korea suggested that self-disposal can be performed 80 days after the generator is used. The purpose of these guidelines is to analyze them by comparing them with the data measured directly with the generator and to study if they are feasible. As a result, the generator with a capacity of 1,000 mCi has the longest half-life, and when tested with a high-radiation Mo(molybdenum) column, the number of days that are below the permitted concentration of body disposal with radioactive waste was 72 days and 71 days that were derived from direct column measurement. The results of the direct study confirmed that the guidelines for in-house disposal in Korea were reasonable, as there were 8 to 9 days of storage compared to the number of in-house disposal days provided in the guidelines.