• Asian-Australasian Journal of Animal Sciences
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• v.18 no.1
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• pp.13-16
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• 2005

This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic HinfI site in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as $HinfI^*$) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of $HinfI^*$ in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and $HinfI^*$ HFABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.

• The KSFM Journal of Fluid Machinery
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• v.11 no.6
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• pp.7-17
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• 2008

Fan performances are obtained with various tip clearance gaps and stagger angles of the rotor. A tested fan is an axial-type fan of which the casing diameter is 806 mm. Two different rotors are applied to this test. One is designed on the basis of the free vortex method along the radial direction and the other is designed using the forced vortex method. The operating conditions are varied to the ultimate off-design point as well as the deign point. Overall efficiency, total pressure and input power are compared with the tip clearance gaps and different stagger angle. The experimental results show that changing of the stagger angle has minor influence to the performance when the same rotor is applied. When the tip clearance gap is less than 5% of the rotor span, the overall efficiency, total pressure loss and input power reduction are varied linearly with the variation of the tip clearance gaps. On the design point, the overall efficiency is decreased to the rate of 2.8-2.9 to the increasing of the tip clearance, but the changing rate of the overall efficiency is alleviated when the fan operates at off-design points. In particular, this rate is more quickly declined on a fan with the rotor designed using the forced vortex method. The result of the total pressure shows that the pressure reduction rate is a 0.08-0.1 according to the tip clearance, and additionally the input power reduction rate is a 0.045-0.065 at design point.

• The Journal of the Korea Contents Association
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• v.8 no.12
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• pp.334-346
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• 2008

The study examined the relationship between servant leadership and organizational commitment and tested the mediating role of the organizational trust between the two variables. Measurement of servant leadership used intactly 23 items of a preceding research. Empirical analysis result can be summarized as follows. Firstly. the servant leadership has positive influence on organizational trust. Secondly. servant leadership has significant influence on affective commitment and normative commitment, but could not reach the significance level for continuance commitment. Lastly, this study was proved there was an partial mediating effect of organizational trust, between the servant leadership and affective commitment, normative commitment, but could not reach the significance level for continuance commitment. And if the organizational trust is higher, the extent of the servant leadership's influence on organizational commitment would be higher.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.799-804
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• 2008

Retrovirus tropism can be restricted by host cell factors such as Fv1, TRIM5${\alpha}$, and LvI that inhibit infection by targeting the incoming viral capsid. The Fv1 gene inhibits murine leukemia virus infection in mice, but the precise mechanism of Fv1-mediated restriction is poorly understood. Our previous studies had demonstrated that Fv1-mediated viral tropism can be determined within the capsid protein at position 114. To study the interaction between Fv1 and CA, we introduced amino acid substitution and deletion at this site in the N-tropic AKV capsid gene. The mutated two-LTR proviral DNAs were introduced into SC-1 cells by transfection. After transfection, cell supernatants collected from transfected cells were tested for host range susceptibility. The result indicated that substitution of amino acids did not alter tropism, but the deletion of 114His produced a virus with unusual tropism. The novel phenotype produced here failed to replicate in Fv1-expressing cells. This mutant virus showing such an extreme restriction pattern would be useful for studying the mechanism of Fv1-mediated restriction.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.552-559
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• 2008

Ethyl (S)-4-chloro-3-hydroxybutyrate is an intermediate for the synthesis of Atorvastatin, a chiral drug used for hypercholesterolemia. A Rhodococcus erythropolisstrain (No.7) able to convert 4-chloro-3-hydroxybutyronitrile into 4-chloro-3-hydroxybutyric acid has recently been isolated from soil. This activity has been regarded as having been caused by the successive actions of the nitrile hydratase and amidase. In this instance, the corresponding amidase gene was cloned from the R. erythropolis strain and expressed in Escherichia coli cells. A soluble active form of amidase enzyme was obtained at $18^{\circ}C$. The Ni column-purified recombinant amidase was found to have a specific activity of 3.89 U/mg toward the substrate isobutyramide. The amidase was found to exhibit a higher degree of activity when used with mid-chain substrates than with short-chain ones. Put differently, amongst the various amides tested, isobutyramide and butyramide were found to be hydrolyzed the most rapidly. In addition to amidase activity, the enzyme was found to exhibit acyltransferase activity when hydroxyl amine was present. This dual activity has also been observed in other enzymes belonging to the same amidase group (E.C. 3.5.1.4). Moreover, the purified enzyme was proven to be able to enantioselectively hydrolyze 4-chloro-3-hydroxybutyramide into the corresponding acid. The e.e. value was measured to be 52% when the conversion yield was 57%. Although this e.e. value is low for direct commercial use, molecular evolution could eventually result in this amidase being used as a biocatalyst for the production of ethyl (S)-4-chloro-3-hydroxybutyrate.

• 한국해양학회지
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• v.18 no.2
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• pp.117-124
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• 1983

Pizza's synthetic model, a modified Wiener filter method, was tested to establish the procedure of desirable interpretive wavelet extraction and its application to the marine seismic exploration using several approaches with a real offshore seismic data of the southeast Asia. Noise spectrum acquisition is difficult and any assumptions for it do not produce interpretive wavelets as good as synthetic model result by Piazza (1979). however the resolution could be improved with spiking deconvoultion and following zero phase bandpass filter, and the testing procedure and evaluatttion of results can hopefully contribute in future study and practical evaluation of Piazza's method.

• Journal of the Korean Regional Science Association
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• v.9 no.1
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• pp.45-63
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• 1993

University enrollment has long been regarded as one of the major factors inducing population concentration in Seoul and the Capital Region of Korea. Consequently, since early 1980's increases in enrollment and new establishments of extention universities beyond the boundary of Seoul, has been promoted, while university enrollment quota in Seoul has been strictly controlled. The degree of actual population dispersal, however, resulting from such a university enrollment policy has not been empirically tested. This paper aims at: First, identifying the trend of population growth and evolution process of the university enrollment policy in the Capital Region; Second, comparing the degree of influence of university enrollment on population concetration factors; Third, measuring actual effect of the enrollment control on population dispersal out of Seoul and the Capital Region. Major findings are as follows: First, only a week correlation between population and university enrollment growth trends was found; Second, the relative degree of influence on population concentration in the Capital Region, were order, in the order of magnitude, the physical amenity factor, the socio-cultural amenity factor, the employment climate factor and the educational factor. Third, and most improtant, based on the comparison of spatial distribution of graduated high schools and current residence of the selected university students, the gap between the two distributions was revealed and the inter-regional student population movement was estimated. The result shows that in Seoul's case about one-half of and in Kyunggi Province's case about one-fifth of university enrollment size, contributes to population concentration into Seoul. Fourth, as to the universities outside of the Capital Region, little effect in the case of universities located within the commuting distance, and a little effect on population dispersal in the case of universities located beyond commuting distance, were found. In sum, it seems clear that university enrollment policy in the Capital Region, especially in Inchon/Kyunggi Province has not been effective on student population dispersal out of Seoul and the Capital Region. Therefore, it is strongly recommended that university enrollment policy be throughly re-examined from its goal to the implementation means.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.7 no.3
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• pp.27-33
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• 1993

This paper presents the &tal controller for driving high frequency voltage fed PWM inverter that carrier frequency is over 2OkHz.We analyzed the conventional PWM to select a proper PWM pattern. as the result, obtained PWM pattern of the controller in which asynchronus staircase sinusoidal waveform is used as reference signal, and variable carrier ratio method was used for PWM control. The PWM controller is designed by fully digital method. Especially, Thk proposed controller is consisted of 8 bit one-chip microprocessor and digital logic. the former is for arithmetic and data processing, and the latter is for PWM pattern synthesis. Therefore, The responsibility and controllability is improved. Also, Data processing capability is improved using proper program to output modulation index with 9 bits. Circuits configuration of digital controller are made up of one chip 8051 and EPLD, and its controllability is tested by operating voltage fed inverter. Harmonics and current waveform is evaluated and analyzed for the voltage fed inverter system.

• Tunnel and Underground Space
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• v.19 no.6
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• pp.479-489
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• 2009

Appropriate investigation of ground condition near excavation face in tunnelling is an inevitable process for safe and economical construction. In this study mechanical parameters from drilling process for blasting were investigated for the purpose of predicting the ground condition, especially rock mass strength, ahead of tunnel face. Rock mass strength is one of the most important factors for classification of rock mass and making a decision of support type in underground construction. Several rock specimens which are considered homogeneous and having different strength values respectively were tested by hydraulic drill machines generally used. As a result, penetration rate is fairly related with rock mass strength among drilling parameters. It is also found that penetration rate increases along with the higher impact pressure even under same rock strength condition. It is finally suggested that new prediction method for rock mass strength using percussive pressure and penetration rate during drilling work can be utilized well in construction site.

• Agricultural and Biosystems Engineering
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• v.4 no.2
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• pp.58-65
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• 2003

An understanding of the cooling requirements of horticultural commodities begins with adequate knowledge of their biological responses. All fresh horticultural products are living organisms, carrying on the many biological processes that are essential to the maintenance of life. The pre-cooling is essential technique for the construction of cold chain system, which is necessary to maintain marketing quality of fresh produces during the transportation and distribution. The purpose of this study is to develop the pressure cooling tunnel using conveyer for the reduction of labor and improvement of pre-cooling efficiency. Performance of developed facility was tested for the strawberries, tomatoes and Chinese cabbages. Cooling ratio as a result of pre-cooling efficiency was 1.57, 1.56 and 1.32 for strawberries, tomatoes, and Chinese cabbages respectively. Cooling ratio decreased with increasing the distance of heat conduction from surface to center. The cooling ratio of Chinese cabbages was lower than that of fruit because of its head and leaf. In aspect of cooling uniformity, there was no significant difference of final temperature among inlet, outlet and middle layers of cold air in fresh produces. After pre-cooling treatment, quality changes were measured for the weight loss, Vit. C content, and titratable acidity. The quality of pre-cooling treatment was better than that of non-treatment and was kept on well during long-term storage.