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Development of Efficient Screening Method for Resistant Cabbage and Broccoli to Plasmodiophora brassicae (양배추 및 브로콜리 뿌리혹병에 대한 효율적인 저항성 검정 방법 확립)

  • Jo, Su-Jung;Shim, Sun-Ah;Jang, Kyoung-Soo;Choi, Yong-Ho;Kim, Jin-Cheol;Choi, Gyung-Ja
    • Research in Plant Disease
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    • v.18 no.2
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    • pp.86-92
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    • 2012
  • Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of $2.5{\times}10^9$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae.

The Analysis on the Export and Import Performance in the Korea Content Industry of the Korea-USA FTA (한·미 FTA의 콘텐츠산업 수출입 효과 분석)

  • Jung, Sang-chul;Ko, Jeong-Min
    • Review of Culture and Economy
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    • v.21 no.2
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    • pp.29-51
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    • 2018
  • The Korea-USA FTA was activated in 2012 and evaluated highly on market-opening among FTA agreements which the Korea government has signed. This paper empirically examines the impact of the Korea-USA FTA on the Korea content industry trade performance with USA. Using ANOVA analysis, the study tested primarily whether there are difference in content exports and imports in a korea-US trade between before and after of Korea-USA FTA in 2012, the year of the entry into force of Korea-USA FTA. Using content industry exports and import data over the period 2007-2015, we find that Korea content export to US after 2012 is significantly different from one before 2012, and there is no difference in import. Based on a regression analysis, we also tested the impact size of Korea content export performance in Korea-USA FTA. The result is that coefficient of FTA dummy variable is not significant, meaning that even though there are some difference in korea content export to US between before and after of Korea-USA FTA, the Korea-USA FTA agreement itself is not a key factor which increase the Korea content export volume to USA market.

Advertising effects depending on picture types of the sights and Facebook user's public self-consciousness (페이스북 포스트의 여행지 사진유형과 사용자의 공적자기의식(public self-consciousness)에 따른 광고효과)

  • Park, Euna;Woo, Yeon-Hoo
    • Journal of the Korea Convergence Society
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    • v.10 no.7
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    • pp.133-139
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    • 2019
  • This study investigated what type of destination image on Facebook increases positive attitude towards the destination and desire to travel. More specifically, we hypothesized that Facebook user's public self-consciousness will moderate the advertising effect, so we tested the difference in attitude towards the destination and willingness to visit and recommend based on the type of destination image (presence/absence of a tourist in the image) and user's public self-consciousness (high/low). As a result, when a tourist was present in the photo, positive attitude toward destination was higher than when absent. In addition, the high public self-consciousness group showed greater attitude toward destination and higher willingness to visit and recommend than low public self-consciousness group when a tourist was present in the image. However, the low public self-consciousness group showed no difference depending on the presence of a tourist. This research not only is academically significant as it experimentally tested advertising effect depending on type of destination image and Facebook user's public self-consciousness, but also holds practical significance as it suggests advertisement method to consider consumer's predisposition.

Construction of a Bark Dataset for Automatic Tree Identification and Developing a Convolutional Neural Network-based Tree Species Identification Model (수목 동정을 위한 수피 분류 데이터셋 구축과 합성곱 신경망 기반 53개 수종의 동정 모델 개발)

  • Kim, Tae Kyung;Baek, Gyu Heon;Kim, Hyun Seok
    • Journal of Korean Society of Forest Science
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    • v.110 no.2
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    • pp.155-164
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    • 2021
  • Many studies have been conducted on developing automatic plant identification algorithms using machine learning to various plant features, such as leaves and flowers. Unlike other plant characteristics, barks show only little change regardless of the season and are maintained for a long period. Nevertheless, barks show a complex shape with a large variation depending on the environment, and there are insufficient materials that can be utilized to train algorithms. Here, in addition to the previously published bark image dataset, BarkNet v.1.0, images of barks were collected, and a dataset consisting of 53 tree species that can be easily observed in Korea was presented. A convolutional neural network (CNN) was trained and tested on the dataset, and the factors that interfere with the model's performance were identified. For CNN architecture, VGG-16 and 19 were utilized. As a result, VGG-16 achieved 90.41% and VGG-19 achieved 92.62% accuracy. When tested on new tree images that do not exist in the original dataset but belong to the same genus or family, it was confirmed that more than 80% of cases were successfully identified as the same genus or family. Meanwhile, it was found that the model tended to misclassify when there were distracting features in the image, including leaves, mosses, and knots. In these cases, we propose that random cropping and classification by majority votes are valid for improving possible errors in training and inferences.

Evaluation of Lipid Accumulation's Inhibitory Activity on 3T3-L1 Cells with Red Yeast Barley Extracts (홍맥 추출물의 3T3-L1세포에 대한 지방 축적 저해 활성평가)

  • Kwon, Gi-Seok;Kim, Byung-Hyuk;Lee, Jun-Hyeong;Hwang, Hak-Soo;Lee, Jung-Bok
    • Journal of Life Science
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    • v.31 no.2
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    • pp.192-198
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    • 2021
  • Red yeast rice has been extensively used as food and traditional medicine for thousands of years in East Asian countries. It is produced by the fermentation of a particular yeast (in general, Monascus purpureus) as rice and various cereals (barley, soybean, etc.). Monascus sp. produces many secondary metabolites during its growth, including pigments, monacolins, and γ-aminobutyric acid. Some metabolites―specifically, monacolin K, γ-aminobutyric acid, dimerumic acid, and monascus pigments―have been reported to lower cholesterol and blood pressure while showing anti-obesity effects. In this study, we investigated the anti-obesity effect of ethanol extract from red yeast barley (RYB) fermented with Monascus sp. BHN-MK 2 on 3T3-L1 cells. The anti-obesity effects of RYB extract were examined: its lipid accumulation inhibitory effect was tested by Oil Red O staining, and obesity-related mRNA expression levels were tested by real-time RT-PCR in MDI stimulated 3T3-L1 cells. The intracellular lipid content of MDI-stimulated 3T3-L1 cells decreased significantly to 5.04%, 12.24%, and 23.52% in response to 200, 400, and 800 ㎍/ml RYB, respectively. Moreovers, we evaluated that RYB extract significantly downregulated the expression of C/EBPα, SREBP-1, and PPAR-γ gene in a dose-dependent manner. As a result, red yeast barley ethanol extracts exerted the strongest anti-obesity effects. Also, the results indicate that red yeast barley could be used as a functional anti-obesity food material.

Impacts of Relative Advantage of Fast Food Restaurant's O2O Service and Consumer Involvement on Consumer Engagement, and Store Loyalty: Focused on MZ Generationsin Untact Consumption Era

  • LEE, Young-Eun;LEE, Yong-Ki
    • The Korean Journal of Franchise Management
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    • v.11 no.2
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    • pp.41-51
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    • 2020
  • Purpose: Fast food franchise companies are trying a variety of innovative services to increase their competitiveness in response to changes in population composition in the fast food market and rapid changes in consumption trends due to technological development. From this point of view, franchise companies that have focused on offline store operations are providing O2O (offline to online) service as a core service for customer convenience. This new attempt is a strategy to increase loyalty by applying an interaction method based on understanding the characteristics of new generation consumers. However, existing studies are focused on the relationship between O2O service and acceptance, so very little is known about how O2O service affects customer loyalty. Therefore, this study examines the impacts of customer involvement and relative advantages of fast food O2O service on customer brand engagement (cognitive and affective engagement) and store loyalty for MZ(Millennials - Z) generations. Research design, data, and methodology: In order to achieve the purposes of this research, several hypotheses were developed. The data were collected from 247 questionnaires in their 16-30s and were analyzed using SPSS 22.0 and SmartPLS 3.0 program. Measurement model analysis was carried out to assess convergent and discriminant validity. Also, common method bias was tested using the values of VIF (variance inflation factor). The hypotheses was tested using structural equation modeling. Result: First, involvement has a positive effect on cognitive and affective engagement. Second, relative advantages have has a positive effect on cognitive and affective engagement. Third, cognitive influences affective engagement. Finally, both cognitive and affective engagement affect store loyalty, but affective engagement has a stronger effect on store loyalty than cognitive engagement. Conclusions: In the process of consumer-brand interaction, it was confirmed that store loyalty was influenced by cognitive and affective engagement sequentially. However, the results show that affective engagement has a relatively stronger on store loyalty than cognitive engagement. Therefore, it is necessary to establish an O2O service strategy to maintain long-term loyal customers by inducing cognitive participation with high-involved consumer, as well as affective interaction, in order to obtain new customers and increase customer loyalty.

Somatic Embryogenesis and Plant Regeneration with Embryogenic Tissue Lines in Pinus densiflora (소나무 배발생조직 라인 별 체세포배 유도 및 식물체 재분화)

  • Kim, Yong Wook;Shin, Han Na;Moon, Heung Kyu
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.449-454
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    • 2011
  • This experiment was conducted to evaluate growth rate among 15 embryogenic tissue lines (ETLs), comparison of maturation on somatic embryos (SEs) with 13 ETLs and efficiency with various concentrations of gelrite on SEs germination in Japanese red pine (Pinus densiflora). In comparison of ETLs growth rate (folds) with 15 lines, the 05-4 line (5.3 folds) showed the highest rate, on the other hand, the lowest one was recorded in the line of 05-37 (1.4 folds). The 13 ETLs were tested for the extent of SEs production. The best production was recorded in the line of 05-4 (39.8/90 mg F.W.). However, most of ETLs, except 5 lines (05-4, 12, 21, 29 and 37), did not produce SEs at all, therefore, big differences in the ability of SEs production existed among the ETLs tested. Effects of various gelrite concentrations for SEs germination with 3 ETLs were also compared. The highest result was obtained from 0.2% gelrite concentration with 05-4 line (47.3%), there was a inclination that the rate of germination was gradually declined over 0.2% gelrite concentration with the 05-4 and 29 lines. respectively. In contrast, in the line of 05-37, no SEs germination occurred on medium with 0.1 or 0.2% gelrite. In conclusion, the growth rate, SEs production and germination frequency were appeared to deeply depended on the ETLs.

Development of LoRa IoT Automatic Meter Reading and Meter Data Management System for Smart Water Grid (스마트워터그리드를 위한 LoRa IoT 원격검침 및 계량데이터 시스템 개발)

  • Park, Jeong-won;Park, Jae-sam
    • Journal of Advanced Navigation Technology
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    • v.26 no.3
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    • pp.172-178
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    • 2022
  • In this paper, water meter AMR(automatic meter reading), one of the core technologies of smart water grid, using LoRa IoT network is studied. The main content of the research is to develop the network system and show the test results that one PC server receives the readings of water meters from multiple households through LoRa communication and stores them in the database, and at the same time sends the data to the web server database through internet. The system also allows users to monitor the meter readings using their smartphones. The hardware and firmware of the main board of the digital water meter are developed. For a PC server program, MDMS(meter data management system) is developed using Visual C#. The app program running on the user's smartphone is also developed using Android Studio. By connecting each developed parts, the total network system is mounted on a flow test bench in the laboratory and tested. For the fields test, 5 places around the university are selected and the transmission distances are tested. The test result show that the developed system can be applied into the real field. The developed system can be expanded to various social safety nets such as monitoring the living alone or elderly with dementia.

Objective and Relative Sweetness Measurement by Electronic-Tongue (전자혀를 이용한 객관적 상대 단맛 측정)

  • Park, So Yeon;Na, Sun Young;Oh, Chang-Hwan
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.6
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    • pp.921-926
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    • 2022
  • Sugar solutions (5%, 10%, 15% and 20%) were tested by seven sensors of Astree E-Tongue for selecting a sensor for sweetness. NMS sensor was chosen as a sensor for sweetness among two sensors (PKS and NMS sensors selected in first stage) by considering precision, linearity and accuracy. Sugar, fructose, glucose and xylitol (5%, 10% and 15%) were tested by E-tongue. The principal component analysis (PCA) result by E-Tongue with seven sensors at 5% concentration level of four sweetners was not satisfactory (Discrimination index was -0.1). On the other hand, the relative NMS sensor response values were derived as 1.08 (fructose), 0.99 (glucose) and 1.00 (xylitol) comparing to sugar. Only the E-Tongue relative glucose response 0.99 was different from 0.5~0.75 of the relative sweetness range reported as the human sensory test results. Considering the excellent precision (%RSD, 1.53~3.64%) of E-Tongue using NMS single sensor for three types of sweeteners compared to sugar in the concentration range of 5% to 15%, replacing sensory test of sweetened beverages by E-Tongue might be possible for new product development and quality control.

Experimental Evaluation of Developed Ultra-low NOx Coal Burner Using Gas in a Bench-scale Single Burner Furnace (Bench-scale 연소로에서 가스 혼소를 통한 초 저 NOx 석탄 버너 개발 연구)

  • Chae, Taeyoung;Lee, Jaewook;Lee, Youngjae;Yang, Won
    • Clean Technology
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    • v.28 no.2
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    • pp.117-122
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    • 2022
  • This study developed and tested an ultra-low NOx burner in an 80 kW combustion furnace. The experiment was conducted in an 80 kW single burner combustion furnace with changing the swirl numbers, total equivalence ratios, and primary/secondary oxidizer ratios. In this study, liquefied natural gas (LNG) was used as an auxiliary fuel to significantly reduce NOx production. In a thermal power plant, the amount of NOx generated during coal combustion is about 300 ppm. However, using the burner tested in this study, it was possible to reduce the amount of NOx generated via LNG co-firing to 40 ppm. If the input amount of the primary oxidizer is enough for the gas to be completely combusted and the gas and coal are added simultaneously, the combusted gas forms a high-temperature region at the burner outlet and volatilizes the coal. As a result, the N contained in the devolatilized coal is discharged. Therefore, when the coal is subsequently burned, the amount of NOx produced decreases because there is almost no N remaining in the coal. If a thermal power plant burner is developed based on the results of this study, it is expected that the NOx generation will be significantly lower in the early stage of combustion.