• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.39-44
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• 2014

We isolated highly-expressed genes in the posterior silk glands of silkworm on a previously study, which one of these was identified as RNA binding protein-1 homologue (RBP-1) gene. In this study, we investigated gene expressional characteristics of the RBP-1 depending on silkworm development stages and several tissues of the larvae, respectively. Northern blot hybridization analysis showed that the RBP-1 gene was expressed high in larval and pupal periods, and highly expressed than endogenous internal control gene (BmA3) on all tested larval tissues. In addition, we isolated and analyzed a phage DNA having 1,660 bp-long promoter region of the RBP-1 gene from a genomic DNA library. To study the RBP-1 gene promoter activity, RBP-1 (-740/+ 30) was amplified by PCR and subcloned into a pGL3 basic vector to generate pGL-RBP1. A luciferase report vector carrying RBP-1 gene promoter (770 bp) was tested by luciferase assay in Sf9 cells. In the result, the RBP-1 gene promoter was more efficient than constitutive promoter (BmA3) by approximately ten percent.

• Journal of Conservation Science
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• v.32 no.3
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• pp.321-331
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• 2016

Textiles damage caused by acetaldehyde($CH_3CHO$) is not clear as compared to other materials. Total 20 specimens were prepared using 4 different materials (silk, cotton, ramie, hemp) after dyed with 4 colors (undyed, red, yellow, blue, black). The specimens were exposed to $CH_3CHO$ gas in the test chamber. First, textile specimens' damage by differert concentration of acetaldehyde(0.1, 0.5, 1, 10, 100, 500, 1000 ppm) was tested. Second, accelerared damage to the textile specimens were tested according to the temperature and humidity conditions at the damage levels. Third, damage of deliberately degraded textile specimens were examined at the damage levels. After the exposure, optical, chemical, and physical evaluation was carried out. As a result, at 1000 ppm/day, the color difference of cotton_yellow has increased. At the condition of $25^{\circ}C-80%$, $30^{\circ}C-50%$, $30^{\circ}C-80%$, the color difference of yellow specimens has increased and grey scale rating has decreased. At $30^{\circ}C-80%$, acetate of cotton_undyed increased and the pH of silk_undyed decreased. In the case of deliberately degraded textile specimens, actetate concentration of black specimens increased. In conclusion, damage to the traditional fabric by acetaldehyde is not impact. However, it is expected that yellow specimens will be bleach and black specimens' actetate concentration will be increase.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.164-170
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• 2008

Coenzyme $Q_{10}$ and six derivatives of coenzyme Qn were synthesized and tested for their inhibitory effects on melanogenesis occurred in murine melanoma (B16/F1) cells and on collagenase/elastase activities as well. As the result, synthetic coenzyme Qn showed a potent inhibitory effect on melanin formation, collagenase and elastase activities in all tested concentrations. Among these synthetic compounds, coenzyme $Q_1$ and coenzyme $Q_2$ potentially inhibited melanin formation and elastase activity when compared to other coenzyme Qn derivatives. For the collagenase activities, all coenzyme Qn derivatives inhibited 80-85% of controls. As compared, coenzyme Qn derivatives exhibited strong inhibitory activities with the decrease of isoprenoid unit number of coenzyme Qn derivatives except for collagenase activity. For the inhibition of collagenase activity, moiety of benzoquinone might be considered as the active functional group. Taken together, coenzyme $Q_1$ and coenzyme $Q_2$ might be used for functional cosmetics.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.155-159
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• 2017

This study was to evaluate the effect of the moisturizing cream containing five kinds of plants extracts for human facial improvements such as hydrating ability, transepidermal water loss and erythema reaction. The antioxidant activities of yellowish natural colorants, extracted from five kinds of plants (Ceramium kondoi, Phellodendron amurense, Carthamus tinctorius, Gardenia jasminoides for. grandiflora and Aphis chinensis) were tested. Twenty-one volunteers were subjected to analyze the effects, and each result was determined by treating the complex cream containing plants extracts on their skin in three times. The moisture content on skin was significantly increased more than 50% by treating the cream containing five kinds of plants extracts. The transepidermal water loss was reduced and the level of the skin erythema reaction was improved after treatment of the complex cream. Survey analysis showed that the volunteers had highly satisfaction on the tested creams. The results suggest that the complex cream containing five plants extracts had an improved effect on facial skin, and therefore can be used as moisturizing ingredients in the related industry.

• Journal of Energy Engineering
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• v.24 no.3
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• pp.89-95
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• 2015

Carbon felts manufactured by (Co)CNF were subjected to heat treatment under different temperatures to use for the electrode of a redox-flow battery. BET and weight loss were tested to investigate the physical properties of the carbon felt according to the heat treatment conditions. SEM and XPS were also analyzed to characterize their surface area. In addition, electrical resistance, CV (cyclic voltammetry) and RFB charge on the electrode properties were examined in accordance with the heat treatment conditions with the discharge performance. The changes of physical properties on the carbon felt surface was confirmed via SEM and BET analysis, The most addition of oxygen functional groups on the carbon felt surface was obtained when one hour heat treatment at $550^{\circ}C$ and it was confirmed by XPS analysis. After resulting the CV tests, the active area of the electrode was the largest at $550^{\circ}C$ heat treatment. The heat treatment experiment of vanadium redox flow battery using the carbon felts were tested at $400^{\circ}C$, $500^{\circ}C$ and $550^{\circ}C$. As a result, the charge-discharge energy efficiency of the heat treatment electrode was 72.9% and 79.8%, at $400^{\circ}C$ and $500^{\circ}C$, respectively. The efficiency of the heat treatment electrode at $550^{\circ}C$ was the best as 79.8% at $550^{\circ}C$.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.4
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• pp.85-94
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• 2016

The purpose of this study was to characterize the coffee ground and its possibility to develop the antifungal activity. pH, EC, and Zn contents of the coffee ground from coffee shops were higher than those of commercial coffee ground, but there was no significant difference in $K_2O$, CaO, MgO, $Na_2O$, Mn contents. The antimicrobial activity of the water soluble extracts from the coffee shop and the commercial coffee ground were tested for six major plant pathogens, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora capsici, Alternaria alternata, and Botrytis cinerea. The result showed that there was reliable antifungal activity against all of tested plant pathogenic fungi. The inhibition effects of coffee ground compost extract on the spore germination and zoospore formation were investigated. Water soluble extracts of the coffee ground compost mixture added with 10% sesame oil cake were significantly inhibited the growth of conidia germination of A. altanata and zoospore formation of P. capsici in vitro. For investigating the functional materials of coffee ground compost, it was measured the total polyphenolic compounds contents with 30 days interval during decomposing coffee ground for 90 days. The total polyphenolic content increased with decomposing periods, and it observed that the highest total polyphenolic content was $0.35{\pm}0.03mg\;GAE/g$ on the 90th day in the coffee ground compost added with 10% sesame oil cake.

• The Journal of the Korea Contents Association
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• v.18 no.8
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• pp.415-433
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• 2018

The purpose of this study is to examine the relationship between collective leadership and employee's work engagement and psychological empowerment. This study tested the impact of collective leadership on employee's work engagement and the mediating role of psychological empowerment between the two variables. And also, this study tested moderating role of self-efficacy between psychological empowerment and work engagement. A total of 237 employees in 7 companies in South Korea participated in this study. The SPSS 21.0 and M-Plus6 statistical programs were used in this study to analyze and statistically process the collected survey data. The result showed that collective leadership positively influence employee's work engagement and psychological empowerment works as mediator in the relationship between collective leadership and employee's work engagement. This results means that collective leadership is a crucial factor to facilitate employee's psychological empowerment toward employee's work engagement. Self-efficacy had moderating effect between psychological empowerment and work engagement. Finally, theoretical and practical implications of the study results have been discussed along with limitations and future direction of the study.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.11
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• pp.6432-6439
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• 2014

The NAUT (Non-contact Air coupled Ultrasonic Testing) technique is one of the ultrasonic testing methods that enables non-contact ultrasonic testing by compensating for the energy loss caused by the difference in acoustic impedance of air with an ultrasonic pulser receiver, PRE-AMP and high-sensitivity transducer. As the NAUT is performed in a state of steady ultrasonic transmission and reception, testing can be performed on materials of high or low temperatures or specimens with a rough surface or narrow part, which could not have been tested using the conventional contact-type testing technique. For this study, the internal defects of spot weld, which are often applied to auto parts, and CFRP parts, were tested to determine if it is practical to make the NAUT technique commercial. As the spot welded part had a high ultrasonic transmissivity, the result was shown as red. On the other hand, the part with an internal defect had a layer of air and low transmissivity, which was shown as blue. In addition, depending on the PRF (Pulse Repetition Frequency), an important factor that determines the measurement speed, the color sharpness showed differences. With the images obtained from CFRP specimens or an imaging device, it was possible to identify the shape, size and position of the internal defect within a short period of time. In this paper, it was confirmed in the above-described experiment that both internal defect detection and image processing of the defect could be possible using the NAUT technique. Moreover, it was possible to apply NAUT to the detection of internal defects in the spot welded parts or in CFRP parts, and commercialize its practical application to various fields.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.135-146
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• 1991

This study was attempted to know the effect of crude extracts of Allium fistulosum on the seed germination and seedling growth in compositae. The result is as follows; The seed germination and seedling growth in the tested compositae were inhibited by the crude extracts from leaf blade, leaf sheath and root of Allium fistulosum. Generally the crude extracts from leaf blade part showed the highest inhibitory effect in the tested parts. The seed germination was inhibited by the crude extracts of Allium fistulosum extracted with 80% methyl alcohol and distilled water. But the crude extracts which had extracted with distilled water indicated more inhibitory effect than that of 80% methyl alcohol. The seedling growth was depressed by the root residues of Allium fistulosum in the soil after harvesting. The crude extracts had the character of stability in light and heat. Some out of the inhibitors contained the volatile substances.

• The Journal of the Korea Contents Association
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• v.16 no.9
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• pp.308-328
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• 2016

The purpose of this study is to examine, at the team level, the relationship between shared leadership and team work engagement and team psychological capital which have rarely been introduced into academic leadership research in Korea. This study tested the impact of shared leadership on team work engagement and the mediating role of team psychological capital between the two variables. And also, this study tested moderating role of task characteristics between shared leadership and team psychological capital. A total of 421 employees of 100 teams in 16 companies in South Korea participated in this study. The SPSS 23.0 statistical program was used in this study to analyze and statistically process the collected survey data. The result showed that high level of shared leadership positively influence team work engagement and team PsyCap works as mediator in the relationship between shared leadership and team work engagement. This results means that shared leadership is a crucial factor to facilitate team's psychological capital toward team's work engagement. However, Task characteristics had no moderating effect between shared leadership and team PsyCap. Finally, theoretical and practical implications of the study results have been discussed along with limitations and future direction of the study.