• Textile Coloration and Finishing
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• v.28 no.3
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• pp.175-182
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• 2016

To investigate dyeing behaviors in accordance with dye structure in cold pad batch dyeing of cotton knit, monochlorotriazine-vinylsulfone bifunctional dyes and monofluorotriazine-vinylsulfone bifunctional dyes were prepared. The spectral property and solubility of the dyes were tested and compared with the imported dyes. In addition, exhaustion behaviors of individual and mixed dye solutions were measured to examine the influence of dye structure on dyeing behavior in cold pad batch dyeing. The substantivity, fixation, migration index and half dyeing time were also calculated for further analysis of dyeing behavior of the prepared dyes. As a result, both dyes exhibited the superior solubilities and satisfactory light absorption properties. Also, monofluorotriazine-vinylsulfone bifunctional dyes showed moderate sensitivity to alkalinity and proper kinetic index values compared with the monochlorotriazine-vinylsulfone bifunctional dyes and the imported dyes. The results indicated monofluorotriazine-vinylsulfone bifunctional structure of the dye is suitable for cold pad batch dyeing.

• Journal of Energy Engineering
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• v.17 no.4
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• pp.212-217
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• 2008

This study carried out the safety evaluation of non-refillable butane can for portable gas range equipped with relief valve for prevention of explosion. The can is heated by electric heater at the real using condition and the extreme condition after installing at a portable gas range for checking the operating pressure and the evaluating suitability of releasing flux. And the possibility of fire or explosion was tested when the gas was released from the relief valve at the real condition. As a result of this safety evaluation test, a non-refillable butane can with relief valve prevents the can from exploding by control of internal pressure.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.2
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• pp.89-97
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• 2004

Many strains of Staphylococcus aureus were isolated from pus samples from primary, secondary, and tertiary medical institutions and were subjected to an antibiotic sensitivity test. Ciprofloxacin, clindamycin, erythromycin, gentamicin, oxacillin penicillin, tetracycline, trimethoprim/sulfamethoxazole, vancomycin and teicoplanin were used for the antibiotic sensitivity test. The strains showed hightest resistance to penicillin(91%), but all of strains tested were susceptible to vancomycin and teicoplanin. The isolated multi-drug(penicillin-tetracycline-ciprofloxacin-clindamycin-erythromycin- oxacillin-gentamicin) resistant S. aureus were analyzed genotypically using an AP-PCR(Arbitrarily Primed polymerase chain reaction) with an arbitrary 3 primers. Based on the result for genotype analysis, the genotypes identified by S1 primer did not coincide with those of S2 or E2 primers. Genotypes identified by S2 primer did not coincide with those of S1 or E2 primers. Also genotypes identified by the E2 primer did not coincide with those of S1 or S2 primers. Therefore, an analysis of AP-PCR test with multiple primers will provide more sensitive identification. A strain from a secondary medical institution and a strain from a tertiary medical institution which showed the same genotype for S1, S2, and E2 primers are required for further epidemiological study.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.446-446
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• 2000

Specific polyclonal and/or monoclonal antibodies (MAbs) to immunoglobulins (Igs) and their subunits have proved to be valuable tools in immunological research and in immunological assays. In this study, we developed and characterized MAbs against flounder serum Igs. To obtain the pure flounder serum Igs, mouse IgG (mIgG) was immunized to flounder. Flounder Igs were purified by using mIgG-agarose affinity column chromatography. The structure of purified flounder Ig was observed, on denatured SDS-PAGE, to be composed of two heavy chains (77 and 72 kd) and two light chains (28 and 26 kd). MAbs were produced by fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells previously primed with the flounder Igs. Finally, three hybridoma clones, FIM 511, FIM 519 and FIM 562 were established to recognize both 2 heavy chains, 26 kd of light chain and 28 kd of light chain, respectively. On the other hand, the flounder immune sera collected on the weekly basis were tested on ELISA and immunoblot analysis whether boosting effect is present in flounder humoral immune system. As a result, the secondary immune response in flounder was ascertained on ELISA, but not on immunoblot analysis. Further, we observed an alteration of serum protein levels following immunization. Our MAbs and basic information on flounder humoral immune system obtained in this study will be helpful to control and monitor the efficiency of fish vaccines and therapeutic process of flounder diseases.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.2
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• pp.131-138
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• 2010

To manage the architecture information of Seoul, aerial photographs have been taken and Photographic Interpretation Status Maps(PISP) have been produced through interpretation process. However PISP of SEOUL has a number of errors and difficulties to update maps, owing to its analogue formation by hand skill. In this study, some digitizing methods are tested to make Digital Photographic Interpretation Status Maps(DPISP). The digitizing methods by scanning maps, using ortho-images and true ortho-images, digital correction plotting process, and LiDAR techniques were used for comparative analysis of PISP. As a result of this study, the author suggest DPISP by the digital correction plotting method is proper for economical efficiency and its accuracy of the architecture information.

• Journal of Microbiology and Biotechnology
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• v.3 no.3
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• pp.161-167
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• 1993

Immobilized metal ion affinity chromatography (IMAC) was used to separate various types of recombinant hirudins from the culture broth. The wild type hirudin exhibited a retention in Cu(II)-chelated affinity chromatgoraphy since it contained a single exposed histidine at position 51. To obtain a stronger retention on an IDA-Cu(II) column, the hirudin variants were genetically engineered to contain one or two histidine (s) more than the wild type. While the affinity of the variants for IDA-Cu(II) ligand increased in comparison to that of the wild type, the antithrombin activities reduced to a certain degree. Cu(II), Ni(II) and Zn(II) ions were applied separately to the metal chelate column to investigate ligand specificity with respect to protein retention. As a result, the Cu(II) chelated chromatography gave the best resolution for all the hirudins tested and appeared to be the only IMAC that could be used generally for the purification of hirudins with a decreasing pH gradient.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.27 no.2
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• pp.113-117
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• 2015

This study presents a 3-dimensional flow-structure interaction analysis of subsea risers in water flows. Two structural connectors (flat and circular couplers) were intentionally devised and numerically tested using ANSYS CFX to investigate how these couplers behave under the water flows. In the flow analysis, the water field was constructed with an inlet, outlet, and symmetric boundary conditions. As a result, the responses (drag coefficients and pressure fields) were obtained and the pressure fields were applied for the structural analysis. Finally, the structural responses (displacements and equivalent stresses) of the risers were measured to demonstrate the efficiency of the riser connectors.

• 한국금형공학회:학술대회논문집
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• 2008.06a
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• pp.35-38
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• 2008

A pattern master and a Ni stamper for 50nm class of patterns are fabricated through e-beam lithography and Ni electroforming process. A model pattern set is designed, which is based on unit patterns of 50nm, 100nm, 150nm and 200nm in length and 50nm in width. The e-beam process is optimized to fabricate designed patterns with some parameters including dose, accelerating voltage, focal distance and developing time. For Ni electroforming to fabricate Ni stamper, a seed layer, a conducting layer, is deposited first on the pattern master fabricated by an e-beam lithography process. Ni, Ti/Ni and Cr are first tested to find optimal seed layer process. Currently the best result is obtained when adopting Cr deposited to be 100nm thick with continuous tilting motion of the master substrate during the deposition process.

• Journal of Korean Society of Disaster and Security
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• v.10 no.1
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• pp.55-60
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• 2017

The dyeing sludge can be weakened by inflow of rainfall or absorption of moisture after it is buried in a waste landfill. This study tested the dyeing sludge and earth/sand mixture to check the problem when the dehydrated dyeing sludge is buried in a waste landfill. When the dyeing sludge was left idle with high water content inside a landfill with poor draining for a long period, the water permeability decreased to around 3/100 level and the compressibility increased by 1.4 times compared to the dyeing sludge at a dyeing factory. The study result indicated that it was important to reduce the water content inside the landfill for stability. Also, the facilities to secure the drainage path and eliminate leachate were needed.

• Journal of Biosystems Engineering
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• v.22 no.2
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• pp.137-150
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• 1997

To investigate the feasibility of a boom sprayer in the paddy field, an experimental boom sprayer for both broadcast and directed spraying to the lower part of rice plants was developed. The droplet deposition characteristics of the boom sprayers were experimentally compared to those of power sprayer. Water sensitive papers(WSP) and a machine vision system were used to evaluate the coverage rate and droplet density. It was shown that the broadcast application by the boom sprayer was the best coverage among the tested sprayers. Coverage tate and droplet density were affected by the distance between nozzles and the sprayer ground speed, The best result was obtained when the distance of 30cm and the speed of 1.7km/hr. The directed application showed inconsistency in overall droplet distribution. The inconsistency was judged to be caused by conflict between plants and boom extenders. The power sprayer showed a very wide range of droplet size distribution, relatively larger droplets and inconsistency in cove The power sprayer was judged to be inadequate for the low-volume precision application because of inconsistency in performance and difficulty in adjusting the spraying rate. Based on the droplet coverage characteristics, it was concluded that the self-propelled boom sprayer for the broadcast application was feasible for an alternative to the power sprayer in case of low volume, precision application in paddy condition.