• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.241-248
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• 2017

BACKGROUND: RNA interference (RNAi) eliminates or decreases gene expression by disrupting the target mRNA or by interfering with translation. Recently, RNAi technique was applied to generate new crop traits which provide protection against pests. To establish the environmental risk assessment protocol of RNAi LMO in lab scale, we developed dsRNA expression system using E. coli and tested acute oral toxicity assay to honey. METHOD AND RESULTS: The dsRNA expression vector, L4440, was chosen and cloned 240 bp of Snf7 and GFP gene fragment. To develop the maximum dsRNA induction condition in E. coli, we tested induction time, temperature and IPTG concentration in media. To estimate the risk assessment of dsRNA to honey bee, it has been selected and cultured with dsRNA supplement for 48 hours according to OECD guideline. As a result, the optimum condition of dsRNA induction was $37^{\circ}C$, 4 hours and 0.4 mM IPTG concentration and the difference between Snf7 and GFP dsRNA molecules from E. coli was not significant in survival and behavior to honey bee. Furthermore, blast search results indicated that effective match of predicted dsRNA fragments were not existed in honey bee genome. CONCLUSION: In this study, we developed and tested the acute oral toxicity of dsRNA using E. coli expression system to honey bee.

• Mycobiology
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• v.35 no.3
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• pp.135-144
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• 2007

The removal efficiency of the heavy metals Zn, Pb and Cd by the zoosporic fungal species Saprolegnia delica and the terrestrial fungus Trichoderma viride, isolated from polluted water drainages in the Delta of Nile in Egypt, as affected by various ranges of pH values and different temperature degrees, was extensively investigated. The maximum removal efficiency of S. delica for Zn(II) and Cd(II) was obtained at pH 8 and for Pb(II) was at pH 6 whilst the removal efficiency of T. viride was found to be optimum at pH 6 for the three applied heavy metals. Regardless the median lethal doses of the three heavy metals, Zn recorded the highest bioaccumulation potency by S. delica at all pH values except at pH 4, followed by Pb whereas Cd showed the lowest removal potency by the fungal species and vice versa in case of T. viride. The optimum bio-mass dry weight production by S. delica was found when the fungus was grown in the medium treated with the heavy metal Pb at pH 6, followed by Zn at pH 8 and Cd at pH 8. The optimum biomass dry weight yield by T. viride amended with Zn, Pb and Cd was obtained at pH 6 for the three heavy metals with the maximum value at Zn. The highest yield of biomass dry weight was found when T. viride treated with Cd at all different pH values followed by Pb whilst Zn output was the lowest and this result was reversed in case of S. delica. The maximum removal efficiency and the biomass dry weight production for the three tested heavy metals was obtained at the incubation temperature $20^{\circ}C$ in case of S. delica while it was $25^{\circ}C$ for T. viride. Incubation of T. viride at higher temperatures ($30^{\circ}C\;and\;35^{\circ}C$) enhanced the removal efficiency of Pb and Cd than low temperatures ($15^{\circ}C\;and\;20^{\circ}C$) and vice versa in case of Zn removal. At all tested incubation temperatures, the maximum yield of biomass dry weight was attained at Zn treatment by the two tested fungal species. The bioaccumulation potency of S. delica for Zn was higher than that for Pb at all temperature degrees of incubation and Cd bioaccumulation was the lowest whereas T. viride showed the highest removal efficiency for Pb followed by Cd and Zn was the minor of the heavy metals.

• Journal of Distribution Science
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• v.13 no.7
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• pp.73-83
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• 2015

Purpose - Recently, many large retailers have been frequently purchasing commercial brands. Not only the quality of products purchased but also the brand images are of concern in this process. Based on this rising trend, commercial brands have become an important issue in the retail business world, along with increasing general interest as well. Thus, this study focuses on the factors affecting commercial brand images and clarifies the impact of the resulting factors as well. First, store images and familiarity, price sensitiveness, and knowledge are presented as the effect factors for commercial brands. Second, the study tries to clarify the effect of commercial brand image on the reliability and loyalty of customers. Research design, data, and methodology - To conduct the study methodology, 250 questionnaires were distributed to retailers who have used large discount stores located in Busan to purchase a commercial brand a total of 234 valid questionnaires were used in the final analysis. To verify the hypotheses, a structure equation formula using Amos 20.0 was calculated. First, prior to the verification of hypotheses, the reliability and feasibility of the questions were tested, and as a result, the value of Cronbach's alpha was higher than 0.7, thereby showing reliability. Additionally, for the verification of the feasibility of the questions, a confirmation factor analysis was implemented. Results - First, variances such as store images, familiarity, price sensitivity, and knowledge were presented as the factors of effect on commercial brands. As a result of the hypotheses verification, all the effect factors presented in this study were confirmed as important variables of commercial brand images. The hypotheses were drawn based on the studies relating to existing commercial brands, and thereby, a result similar to previous studies was produced. However, the effect factors of the commercial brand image presented in this study shaped a new study model based on the previous studies. Second, it was found that commercial brand images had positive effects on the reliability and loyalty of customers. This study presented reliability and loyalty as the resulting factors of commercial brand image because they are the most important factors in relation to customer behavior and variables that can suggest marketing points for distribution businesses. Conclusions - This study focused on clarifying the factors that are important effect factors for commercial brand images. As a result, all the hypotheses were confirmed in this study, indicating a meaningful result, and thereby suggesting many points that can be presented to distribution businesses. First, the proper strategies should be developed based on the hypothesis that a store image, the familiarity of customers with brands, and the price sensitiveness and knowledge level of customers have an important effect on the choice of commercial brands. Additionally, the formation of such favorable images will have positive effects not only in terms of customer trust in the commercial brands but also in terms of their loyalty.

• Korea Journal of Hospital Management
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• v.11 no.4
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• pp.82-103
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• 2006

The purpose of this study is to analyse statistically the relation between medical service quality and managerial performance. And then the way of analysis is the regression analysis that independent variable is service quality, dependent variable is the volume of revenue, the number of patients and the rate of beds utility, and dummy variable is the number of beds, ownership and region. The sample hospitals were the 113 hospitals on general hospitals more than 300beds which were consisted of 20 public hospitals, 41 corporate hospitals and 52 college hospitals, and also distributed 67 hospitals on big city and 46 hospitals other city. The sample hospitals were selected from the Korean Hospital Association and the data of the year 2003 and 2004. The collected data was analyzed using the SPSSWIN 10.0 version, and the study hypothesis was tested using regression analysis. The findings of this study are summarized as follows. First, as a result of analysing hypothesis 1. In the study of the relationship between the service quality and the revenue, it was verified that the more service quality in last year became the more revenue in the year. But the dummy variable, ownership and region, rarely related to hospital revenue. Therefore it means that the more service quality is connected to the more revenue on the large number of beds hospitals. Second, as a result of analysing hypothesis 2 & 3. In the study of the relationship between the service quality and the number of patients, it was verified that the more service quality in last year became the more outpatients in the year. But there was no verified to inpatients. It seems to be the reason why a hospital has operated the fixed number of beds approved by the public office. So there are no free to expand beds according to the number of inpatients as much as inpatients are increasing. Third, as a result of analysing hypothesis 4 & 5. In the study of the relationship between the service quality and the rate of beds utility, it was verified that the more service quality in last year became the shorter of average stay of length in the year. Especially it has influenced much more on a hospital which was the large number of beds, the corporate and the college, but the region. But it was denied that the more service quality became the more the beds turnover. As a result of this study, it shows that the service quality in the last year has importantly influenced on a hospital managerial performance in the year. Estimating the service quality of each hospital, most patients have selected the hospital they want. And the hospital need to keep the number of patients for the proper management. So this result of the study means that a hospital must improve the service quality for keeping efficient management.

• Fibers and Polymers
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• v.7 no.4
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• pp.442-445
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• 2006

The purpose of this study was to compare the subjective fabric hand evaluation and wear comfort of PET treated by Ultraviolet and to evaluate the subjective results from the investigation of microclimate temperature. The subjective hand evaluation was performed by 20 subjects (age: 20-25) with 5-point scale questionnaires to investigate the change of PET knit fabrics treated for different times, specifically, 0, 30 and 90 minutes. The questionnaires were composite with 8 questions; roughness, smoothness, wetness, stiffness, coolness, touch, preference, and comfort. In order to evaluate sensations of wear comfort, we made garments with UV treated fabric and five female students were tested. They walked at the speed of 6.7 km/ hr for 15 minutes in an environment, which was controlled at $29^{\circ}C,\;75{\pm}5%$ RH. Before and after exercising, the microclimate under clothing and subjective wear comfort was measured. The results of subjective evaluation of fabric hand were that untreated and treated for 30 minutes PET were recognized as similar and have a good evaluation on comfort, preference, and touch. According to the result of wear comfort, clothing treated by UV for 90 minutes had the lowest value on the thermal and humidity sensations. In addition, the value of tactile and comfort sensation was the highest on the clothing treated by UV for 90 minutes. In the case of objective evaluation, PET treated for 90 minutes was the lowest on microclimate humidity. PET irradiated by UV for 90 minutes was more 'cool' in thermal sensation and more 'dry' in wet sensation. Accordingly, it was consistent with the result of subjective wear comfort.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.22 no.3
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• pp.80-94
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• 2009

Objective : The purpose of this clinical research is the investigation of the effects between acupuncture group(control group) and NURIEYE-1 group (experimental group) in myopia Methods : Comparison clinical study was conducted with the randomly arranged 32 patients for acupuncture group(control group) and NURIEYE-1 group(experimental group). For effectiveness evaluation, eye sight test, MQOL and DITI were tested three times with visits for every 4 weeks. With SAS, Statistical analysis was performed by using paired t-test and two sample t-test. Statistical significance was achieved with less than 5% probability (p<0.05). Result : 1. There was no significant effect from the eye sight comparison between the two group. We had improved results in average with MQOL, however, there was no statistical significance. Though there was also no significant result with DITI average comparison between two group, we witnessed significant effect in some part of MQOL. 2. Nevertheless Periodical comparison test showed weak significance with eye sight and DITI, significant effect was found in some part of MQOL. 3. With periodical comparison tests, there were significant effects in eye sight, MQOL and DITI with the experimental groups and control groups, respectively. Conclusion : There was no significant eye sight improvement. However, with acupuncture point stimulation, we think there are some positive effects for the relaxation of excessive stress in eyes.

• Journal of Sasang Constitutional Medicine
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• v.18 no.1
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• pp.75-90
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• 2006

1. Objectives The Sasang Constitution has been studied in many parts and one of the most common inquiries is the self-reporting questionnaire. But the established investigation has the problem that the respondents are not fully aware of the questionnaires and answer them. To settle this problem, it's been made new questionnaires through two steps. 2. Methods At first step, the testers ate classified into Yangin(陽人) and Eumin(陰人). And then, Taeyangin is distinguished from Soyangin likewise Taeumin is told from Soeumin. It's called 'The Two Step Questionnaire for the Sasang Constitution Diagnosis(TS-QSCD)'. This new method is tested by 160 testers and Woosuk university students whose constitution ate confirmed to make clear clinical uses 3. Results and Conclusions Questionnaire Sasang Constitution corresponds with The Real Sasang Constitution at the rate of 71.87%. Only 5.63% has shown an error at the first step. Every experimenters by constitutions, the 56 objects of 74 Taeumin are confirmed the same as it is. It has 84.85% accuracy. The 27 objects of 32 Soeumin come to the same thing and it has 69.23% accuracy. Similarly, the 32 objects of 42 Soyangin come to the same conclusion and 72.73% accuracy high As the result, in comparison to the Real Constitution with the Constitution by questionnaires, the accord comes out Kappa Indices 0.6427866. This result is comparatively high. Chi-square Test can be shown statistical meaning as well.

• Journal of Advanced Navigation Technology
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• v.14 no.3
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• pp.367-374
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• 2010

In this paper, the frequencies sharing possibility between TA(TalkAround) Radios and RFID system in 900MHz frequency band have been studied. The modulation technic for FHSS(Frequency Hopping Spread Spectrum) of TA is digital FSK and RFID system adapts digital ASK modulation technic. The communication mode of the tested RFID system for frequencies sharing possibility was FHSS. The field test was performed by five scenarios. The RFID field testing system for the frequencies sharing possibility was composed of four tags and two main devices made in USA. Four TA and Egilant spectrum analyzer was used for testing. The frequencies sharing possibility by differentiated modulation was theoretically analyzed. As result of simulation, it was proved that the frequencies sharing between each other modulation system is possible. And also as result of field testing it was confirmed that the signal of TA Radios have no effect on the signal of RFID system.

• Journal of the Korean Society of Propulsion Engineers
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• v.16 no.2
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• pp.1-9
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• 2012

Applicability of regenerative cooling in 2,500 N-class bipropellant thruster using hydrogen peroxide and kerosene was considered for improvement of performance and application in various missions. Calculation was performed by one dimensional approach using hydrogen peroxide as a coolant. The heat flux of thruster at nozzle throat was estimated at 18 - 20 MW/$m^2$. Designed cooling channel width and height were 2.5 mm and 0.5 mm, respectively. Based on designed cooling channel configuration, flat plate model was manufactured and tested for estimation of pressure drop in cooling channel, and CFD analysis was compared with the test result. The maximum error between CFD analysis and experimental result was approximately 13% and average error was approximately 5%.

• The Journal of the Korea Contents Association
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• v.20 no.3
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• pp.31-40
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• 2020

As a result of reenforcement of livestock industry licensing and livestock related vehicle registration system, pig farm are mandatory for setting stop bar before pig farm entrance and maintaining the records of inlet and outlet to and from pig farm. Also, the first step to the pig farm biosecurity is the control of entrance human, vehicles, animals and the related records including entrance and sterilization. In this study, the pig farm entrance control system using RFID technology and web-server system was developed. For human entrance, contact type RFID reading system(13.56MHz) was developed. And the 900MHz RFID system was used to detect vehicle entrance. The test result shows that system actuating, recognition, saving records to the web server was successful. Then, the full system of human entrance recording unit, vehicle entrance recording unit, entrance control system, human sterilizing booth containing a tablet for inputting visitor's record and sterilization record was tested. The records were wrote to web server DB through the data management web-program. Performance test shows the entrance control and data management in server was successfully operated.