• 제목/요약/키워드: terminal segment

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DNA와 상호작용에서 T4 endonuclease V의 C-말단 부위의 역할에 관한 분광학적 연구: 핵자기공명과 형광 실험 (The Spectroscopic Study on the Role of C-terminal Region of T4 endonuclease V in the Interaction with DNA: NMR and Fluorescence Experiment)

  • 유준석;임형미;임후강;신정휴;이봉진
    • 약학회지
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    • 제40권2호
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    • pp.193-201
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    • 1996
  • In order to study the role of C-terminal aromatic region of T4 endonuclease V in the interaction with substrate DNA, NMR and Fluorescence spectrum were recorded. Analysis of flu orescence emission spectra showed that C-terminal region of T4 endonuclease V is in or very near the binding site. In the HSQC spectrum of $^{15}N$-Tyr-labeled T4 endonuclease V*DNA complex, the broadening of a peak was observed. It is presumed that this peak corresponds to one among three tyrosine residues which belong to the WYKYY segment of C-terminal region of T4 endonuclease V. Interactions of peptide fragments consisting of C-terminal residues of T4 endonuclease V with DNAs(TT-, T^T-DNA) were investigated by NMR and Fluorescence experiment. The results suggest that two peptide fragments themselves bind to DNAs and their binding pattern is not an intercalation mode.

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동문동물 1신종, 표창자라목벌레 (Echinoderes lanceolatus)의 기재(동문동물문, 원통자라목벌레목, 가시자라목벌레과) (Echinoderes lanceolatus, a New Kinorhynch from Korea (Kinorhyncha, Cyclorhagida, Echinoderidae))

  • Chang, Cheon-Young;Song, Young-Hee
    • Animal Systematics, Evolution and Diversity
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    • 제18권2호
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    • pp.203-211
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    • 2002
  • 한국의 동해안과 남해안에서 채집한 동문동물 1신종(Echinoderes lanceolatus)을 기재한다. 표본은 조하대의 저질과 해조류를 플랑크톤 넷트에 걸러 채집하였다. 이 종은 제3마디 흉판 중앙부 양측의 커다란 각흔(殼痕) 제4마디 흉판의 희미한 중앙연접 자국. 각각 해당 몸통 마디보다 약간 짧은 제6-10정중등가시. 암컷 제9-10마디 흉판의 중괄호형 근흔(筋痕), 마지막 복판의 뒷가장자리가 곧은점, 그리고 표창처럼 날카롭고 긴 제13마디의 등돌기가 특징이다.

Sexual Dimorphism on the Genus Paracalanus(Copepoda: Paracalanidae) in Korean Waters

  • Cho, Kyu Hee;Lee, Won Choel;Kim, Saywa
    • 한국환경생물학회:학술대회논문집
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    • 한국환경생물학회 2003년도 학술대회
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    • pp.116-120
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    • 2003
  • The genus Paracalanus Boeck, 1864 dominate zooplankton communities in waters off Korean peninsula. Zooplankton samples collected from four waters (Uljin, Hadong, Youngkwang, Cheju) were sorted for specimens belonging to the genus Paracalanus. Paracalanus sp. regarded to p. paMus or p. indicus were examined. Body of male has cephalosome dorsal hump (CDH). Urosomites have four segments in female and five segments in male. In male, antennule is symmetry and fused without geniculation. The male antennule carries more aesthetascs than those in female antennule. Female P1-P4 is similar to those of male in seta and spine formular. Spinules on surface of legs are different each other. The female P5 is symmetrical, and composed of 2 segment. In male, it is aymmetrical and left leg composed of 5 segments, right leg 2-segmented. Male found patch of fine spinule on terminal inner surface of outer process on fifth segment. Sexual dimorphism appear saliently in mouth appendages. Mandible lacks of gnathobase; maxillule is apparently reduced; maxilla degrades it i]l female and remains vestiges between maxillule and maxilliped; maxilliped terminal part indistinctly articulated and carrying behind three strong plumose setae. With the absence of gnathobase in male, we conclude that male Paracalanus sp. does not feed.

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ErmSF에서 특이적으로 발견되는 N-terminal end region에 존재하는 1-25번째 아미노산을 함유하는 peptide segment의 효소 활성에서의 역할 (Functional Role of Peptide Segment Containing 1-25 Amino Acids in N-terminal End Region of ErmSF)

  • 진형종
    • 미생물학회지
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    • 제42권3호
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    • pp.165-171
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    • 2006
  • ERM protein 은 23S rRNA의 $A_{2058}$에 dimethylation시킴으로써 $MLS_B$계 항생제의 부착을 저해하여 항생제의 활성을 억제하는 내생인자 단백질이다. ERM 단백질의 하나인 ErmSF의 N-말단부위(N-termimal end region, NTER)에 존재하는 1-25번째 아미노산을 함유하는 펩타이드의 활성에서의 역할을 알아보기 위해 이률 제거한 변이 단백질을 표현하는 유전자를 클로닝하고 대장균에서 수용성 단백질로 12.65 mg/L culture의 수율로 대량생산하였다. 이렇게 대량생산된 단백질의 활성을 in vivo와 in vitro에서 확인하였다. 그 결과 in vitro에서 야생형(wild type)의 단백질에 비해 15%의 활성이 감소한 것을 확인하였고 이는 제거된 펩타이드가 기질과 상호작용하여 효소의 활성에 영향을 미친다는 것을 시사하고 있다. 이렇게 감소된 효소의 활성은 생체 내(in vivo) 활성에도 적용되어 처음에는 변이 단백질을 함유하는 세포가 항생제의 작용에 의하여 성장억제를 받지만 시간의 경과와 함께 내성을 회복하여 밤샘 배양하였을 경우는 야생형 단백질을 함유한 세포와 동일한 내성 즉 항생제에 의한 성장억제지역(inhibition zone)을 전혀 나타내지 않는 것으로 밝혀졌다.

Six new species of Copepoda (Clausiidae, Pseudanthessiidae, Polyankyliidae) associated with polychaetes from Korea

  • Kim, Il-Hoi
    • Journal of Species Research
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    • 제3권2호
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    • pp.95-122
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    • 2014
  • Six new species of copepods associated with polychaetes are described from Korea. Included are Indoclausia bipartita n. sp., Clausia parva n. sp., and Maxilliclausia propria n. gen. n. sp. in the Clausiidae; Pseudanthessius excertus n. sp. in the Pseudanthessiidae; and Polyankylis ovilaxa n. sp. and Sewelloya plana n. gen. n. sp. in the Polyankyliidae. The new genus Maxilliclausia has a peculiar maxilla in which the distal segment forms a sucking disk, a non-prehensile antenna, vestigial legs 3 and 5, and lacks a maxilliped and leg 4. The two new species of the Polyankyllidae are discovered as external associates of terebellid polychaetes. The new genus Sewelloya has a simple female maxilliped bearing only two small setae on its terminal segment, an uniramous leg 4 bearing 1-segmented exopod, and a reduced leg 5 represented by three setae. The genera Terebelliphilus Kim, 2001 which was originally placed in the Sabelliphilidae, and Octophiophora Stock, 1988 and Stockia Sebastian and Pillai, 1974, both of the latters with uncertain familial positions, are transferred to the Polyankyliidae. A key to five genera of the Polyankyliidae are provided.

A New Species of the Genus Cavernocypris Hartmann, 1964 (Crustacea, Ostracoda) from Gosu cave in Korea

  • Kim, Byung-Woo;Kwon, Jung-Kyun;Lee, Won-Cheol
    • 한국전자현미경학회:학술대회논문집
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    • 한국현미경학회 2004년도 제35차 추계학술대회 및 제2회 HVEM 이용자 워크샵
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    • pp.105-110
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    • 2004
  • Cavernocypris gosuensis n. sp. (Cyprididiae, Cypridopsinae), a new subterranean ostracod species is described with figures of female valves, mouthparts, appendeges and SEM photos from Gosu cave, Danyang-Gun, Chungcheongbuk-Do in Korea. The new stygobiont speices is sixth of the genus Caveroncypris Hartmann, 1964 that characterized by both valeves with a median concave part in ventral view and two groove lines in dorsal view, the reduction of the furca, which is flagellum like in female and the left valve ventrally overlaps the right valve when the carapace is closed and can be distinguished from its congers by left valve with 6 muscle scars in innerpart, mandible palp with one claw and 3 setae on the terminal segment and without seta on the exterodistal border of segment III. The animals were collected within pH 7.31-8.74, temperature ($^{\circ}C$) 13.0-14.6 and dissolved oxygen (mg/l 6.75-14.84 from Aug.2003 to Sep.2004.

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한국산 키조개에서 채집된 Anthessius (Copepoda, Poecilostomatoida , 'Anthessidae)의 1 신종 (A New Species of Anthessius (Copepoda, Poecilostomatoida , Anthessiidae) from the Pen Shell, Atrina pectinata (Linn) in Korea)

  • 서해립;최상덕
    • Animal Systematics, Evolution and Diversity
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    • 제7권1호
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    • pp.45-54
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    • 1991
  • 한국 연안에서 서식하는 키조개의 외투강에서 채집된 요각류 1종이 Anthessius속의 신종임이 확인되어 A. atrinae로 명명하였다. 본 종은 제2안테나 마지막 마디에 있는 3개의 claw와 제 4외지 마지막 마디의 강모식 II, I, 5를 갖는 점과 caudal ramus의 형태의 차이에 의하여 다른 종과 구별된다.

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Characterization of the Putative Membrane Fusion Peptides in the Envelope Proteins of Human Hepatitis B Virus

  • Kang, Ha-Tan;Yu, Yeon-Gyu
    • Bulletin of the Korean Chemical Society
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    • 제28권10호
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    • pp.1756-1762
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    • 2007
  • Envelope proteins of virus contain a segment of hydrophobic amino acids, called as fusion peptide, which triggers membrane fusion by insertion into membrane and perturbation of lipid bilayer structure. Potential fusion peptide sequences have been identified in the middle of L or M proteins or at the N-terminus of S protein in the envelope of human hepatitis B virus (HBV). Two 16-mer peptides representing the N-terminal fusion peptide of the S protein and the internal fusion peptide in L protein were synthesized, and their membrane disrupting activities were characterized. The internal fusion peptide in L protein showed higher activity of liposome leakage and hemolysis of human red blood cells than the N-terminal fusion peptide of S protein. Also, the membrane disrupting activity of the extracellular domain of L protein significantly increased when the internal fusion peptide region was exposed to N-terminus by the treatment of V8 protease. These results indicate that the internal fusion peptide region of L protein could activate membrane fusion when it is exposed by proteolysis.

A simulation study on TCP performance for constrained IoT networks

  • Chansook, Lim
    • International Journal of Internet, Broadcasting and Communication
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    • 제15권1호
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    • pp.1-7
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    • 2023
  • TCP is considered a major candidate transport protocol even for constrained IoT networks due to its ability to integrate into the existing network infrastructures. Since TCP implementations such as uIP TCP often allow only a single TCP segment per connection to be unacknowledged at any given time due to resource constraints, the congestion control relies only on RTO management. In our previous work, to address the problem that uIP TCP performs poorly particularly when a radio duty cycling mechanism is enabled and the hidden terminal problem is severe, we proposed a RTO scheme for uIP TCP and validated the performance through Cooja simulation. In this study, we investigate the effect of other factors that were not considered in our previous work. More specifically, the effect of traffic intensity, the degree of the hidden terminal problem, and RDC is investigated by varying the offered load and the transmission range, and the RDC channel check rate. Simulation results imply that we need to further investigate how to improve TCP performance when the radio duty cycling mechanism is used.