• Asian-Australasian Journal of Animal Sciences
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• v.23 no.10
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• pp.1369-1379
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• 2010

This study investigated purification and milk-clotting activity of the enzymes produced by Bacillus subtilis var, natto and Rhizopus oligosporus compared with that of commercial rennet. The clotting time, viscosity, tension and microstructure of the curd and electrophoretic patterns of milk proteins were determined. The milk-clotting activity/proteolytic activity ratios (MCA/PA ratio) of B. subtilis, R. oligosporus and commercial rennet were also compared. The results revealed that the curd formed by the commercial rennet had the highest viscosity and curd tension and the shortest clotting time among the three enzymes. However, curd produced by Rhizopus enzymes was ranked as second. From the MCA/PA ratio and electrophoretogram analyses it could be concluded that the enzyme produced by B. subtilis had the highest proteolytic activity, while the commercial rennet had the highest milk-clotting activity. Observations of microstructures of SEM showed that the three-dimensional network for curd formed by commercial rennet was denser, firmer and more smooth. The milk-clotting activity, specific activity, purification ratio and recovery of the purified enzymes produced by both the tested organisms were also determined with ion exchange chromatography and gel filtration.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.2
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• pp.148-153
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• 2018

In this study, the nonlinear time history analysis of seismic retrofitted structures with TS damper for seven ground motion records was conducted for the purpose of verifying the seismic strengthening effect of TS seismic retrofitting method. Through comparison of the interstory drift ratio and the energy dissipation amount of the non - reinforced structure obtained and those of retrofitted structures with TS damper from the nonlinear time history analysis, the interstory drift ratio was reduced by about 30% and the amount of energy dissipation through the structure was halved. As a result, it was confirmed that the damping performance of the TS seismic retrofitting method is excellent.

• Geomechanics and Engineering
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• v.7 no.5
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• pp.477-493
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• 2014

This study examines the behaviour of single micropiles subjected to axial tension or compression load in collapsible loess under in-situ moisture content and saturated condition. Five tension loading tests and five compression loading tests on single micropiles were carried out at a typical loess site of the Loess Plateau in Northwest China. A series of laboratory tests, including grain size distribution, specific gravity, moisture content, Atterberg limits, density, granular components, shear strength, and collapse index, were carried out during the micropile loading tests to determine the values of soil parameters. The loess at the test site poses a severe collapse risk upon wetting. The tension or compression load-displacement curves of the micropiles in loess, under in-situ moisture content or saturated condition, can generally be simplified into three distinct regions: an initial linear, a curvilinear transition, and a final linear region, and the bearing capacity or failure load can be interpreted by the L1-L2 method as done in other studies. Micropiles in loess should be considered as frictional pile foundations though the tip resistances are about 10%-15% of the applied loads. Both the tension and compression capacities increase linearly with the ratio of the pile length to the shaft diameter, L/d. For micropiles in loess under in-situ moisture content, the interpreted failure loads or capacities under tension are 66%-87% of those under compression. However, the prewetting of the loess can lead to the reductions of 50% in the tensile bearing capacity and 70% in the compressive bearing capacity.

• Journal of the Korea Concrete Institute
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• v.26 no.5
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• pp.581-589
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• 2014

This paper presents the tension stiffening behavior from experimental results of each 6 amorphous steel fibers and normal steel fibers reinforced direct tensile specimens with the main variables such as cover thickness to bar diameter ratio. A tension stiffening effect for steel fiber reinforced RC tension members improve on the increase in cover thickness, and also amorphous steel fiber is usually superior to normal steel fiber. The reinforcement of steel fibers controlled the splitting cracks and led to significant increase in the tension stiffening effect. In particular, if cover thickness is more than twice the bar diameter, the amorphous steel fiber reinforced specimen is controlled the splitting crack and increased the tension stiffening effect. And, the tension stiffening effect of amorphous steel fiber reinforced concrete tension members is different to current structural design code provision.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.109-114
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• 2005

A bacterium capable of emulsifying hydrocarbon, n-hexadecane, and decreasing surface tension of the culture media using oil collapsing method was isolated. The bacterium was partially identified as Bacillus sp. and named BJS-51. n-Hexadecane was the most effective carbon source for production of biosurfactant. Surface tension was decreased from 76 dyne/cm to 31 dyne/cm and CMD (critical micelle dilution) had the highest value of 5.7 at 3% n-hexadecane. Ammonium phosphate was the most effective nitrogen source, when C/N ratio was 60, surface tension and CMD were 29 dyne/cm and 9.2, respectively. Optimum pH and temperature were 7.2 and $30^{\circ}C$, respectively. Produced biosurfactant was extracted and purified using organic solvent extraction method and preparative HPLC systems. After analysis by various color reaction, this biosurfactant was identified as lipopolysaccharide. Surface tension and CMC (critical micelle concentration) of purified biosurfactant were 27 dyne/cm and 0.08 g/l, repectively. CMD was 9.2, so the yield of biosurfactant was about 0.74 g/l at the optimal conditions. The biosurfactant was very stable at wide range of $pH\;2{\sim}12$ with surface tension $29{\sim}31\;dyne/cm$ and showed $29{\sim}30\;dyne/cm$ of surface tension after heat treatment at $100^{\circ}C$ for 60 min.

• Journal of Ocean Engineering and Technology
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• v.14 no.2
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• pp.99-105
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• 2000

This paper describes the effect of specimen size on fatigue crack growth rate for the offshore structural high-tensile-strength steel BS4360 and machine structural steel SM45C. The purpose of the present study is to investigate the effect of stress ratio aspect ratio specimen width and specimen thickness of the fatigue crack growth behavior. Compact tension specimens with a LT orientation for BS4360 and SM45C steels were used, All testing was done at constant stress intensity factor range controlled fatigue crack growth condition. The investigation demonstrates that the fatigue crack growth rate is increased with increasing stress ratio and specimen thickness and is decreased with increasing specimen width. The fatigue crack growth rate is unaffected by aspect ratio until a/W=0.50 but is increased by increasing spect ratio from a/W=0.55.

• Magazine of the Korea Concrete Institute
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• v.6 no.2
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• pp.180-187
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• 1994

Sixteen reinforced concrete beams were tested statically up to failure to investigate the arch action. Major variables were the shear span to depth ratio, steel ratio and existence of stirrups.The arch action in reinforced concrete beams started when flexural cracks appeared at the center of the span. Due to the reduction of internal moment arm length by the development of arch action, the measured steel tension was significantly higher than the calculated. As the shear span to depth ratio arid steel ratio decrease, the arch action in reinforced concrete eams increases. Over the entire length the force in the steel of no web reinforced beams having smaller a /d ratio than 3 was constant because the beams acted as a tied arch.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.5
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• pp.1-10
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• 2003

The purpose of this study was to verify the shore margin protection effect of a root system developed from direct sticking of Salix gracilistyla Miq., focusing on the reinforcement of soil shear strength. The materials were 20cm long sticks whose average diameter and weight were 7.52mm and 14.58g respectively, and sandy loam(Sand 60.36%, Silt 28%, Clay 11.64%), whose maximum dry weight(${\gamma}$$_{dmax}$) was 1.59gf/㎤ at the water ratio( $W_{opt}$) 13.8%. The direct shearing test(KS F 2343) was applied to cylindric columms(diameter 132mm) of pure soil and two years old root reinforced soil. At each condition of vertical stress, 10N/$ extrm{cm}^2$, 14.41N/$\textrm{cm}^2$ and 18.82 N/$\textrm{cm}^2$, five soil+root columns were sheared. After shear tests, the root area ratio and soil moisture on the shear plane were measured. The results of this research were as follows: 1. The average of root area ratio was 1.86% and the soil moisture 14.67%. 2. Two years old root system was found to increase the soil shear strength of pure soil in terms of Cohesion(C) and Inner friction Angle($\phi$) as follows. 3. The relationship between root area ratio and the increased shear strength can be presented with the following equation, $\Delta$S ≒ 0.33ㆍ TrㆍAs/A $\Delta$S : Increased Shear Strength Tr : Average Tension Strength of Root, Ar/A : Root Area Ratioioage Tension Strength of Root, Ar/A : Root Area Ratio

• Nuclear Engineering and Technology
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• v.27 no.6
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• pp.825-832
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• 1995

The effect of loading variables and temperature on fatigue crack growth rate in SA508 Cl.3 nuclear pressure vessel steel was investigated in air environment Crack growth rate tests on compact tension specimen of thickness 12mm were conducted by using sinusoidal waveform. The crack length was monitored by compliance method. Test conditions were at 0.1 and 0.5 of load ratio, at 1 and 10 Hz of loading frequency, and at room temperature to 40$0^{\circ}C$. At the lower temperatures, the fatigue crack propagation was not affected by the frequency and temperature, while at the higher temperatures above 12$0^{\circ}C$, fatigue crack growth rate increased with decreasing loading frequency and increasing temperature. This accelerated fatigue crack propagation was associated with the increase of oxidation rate at the ahead of crack tip. Fatigue crack growth rate increased with in-creasing the load ratio. The effect of load ratio was more significant at the lower temperature, while the dependence on load ratio decreased with increasing temperature. The sensitivity of load ratio to temperature can be explained by crack closure with the oxidation process.

• Composites Research
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• v.23 no.5
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• pp.22-29
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• 2010

This paper describes the evaluations of tension-compression fatigue characteristics and life for glass fiber/epoxy laminate composite applied to railway bogie to reduce weight. Test samples of tension-compression fatigue were composed of glass fiber/epoxy 4-harness woven laminate composites with different stacking sequence of warp-direction, fill-direction and ${\pm}45^{\circ}$-direction. The tension-compression fatigue test was conducted with stress ratio (R) of -1 and frequency of 5Hz. Goodman diagram were used to evaluate the fatigue characteristics and life of glass fiber/epoxy 4-harness satin woven laminate composite. Anti-buckling jig was designed to prevent buckling of specimen under compression load. The test results showed that the fatigue characteristics of glass fiber/epoxy 4-harness satin woven laminate composite with stacking sequence of warp-direction had a good performance in comparison with that of SM490 used to conventional metal railway bogie.