• Structural Engineering and Mechanics
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• v.17 no.3_4
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• pp.539-556
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• 2004

In this paper, the design, fabrication and characterization of a piezoelectric friction damper are presented. It was sized with the proposed practical procedure to minimize the story drift and floor acceleration of an existing 1/4-scale, three-story frame structure under both near-fault and far-field earthquakes. The design operation friction force in kip was numerically determined to range from 2.2 to 3.3 times the value of the peak ground acceleration in g (gravitational acceleration). Experimental results indicated that the load-displacement loop of the damper is nearly rectangular in shape and independent of the excitation frequency. The coefficient of friction of the damper is approximately 0.85 when the clamping force on the damper is above 400 lbs. It was found that the friction force variation of the damper generated by piezoelectric actuators with 1000 Volts is approximately 90% of the expected value. The properties of the damper are insensitive to its ambient temperature and remain almost the same after being tested for more than 12,000 cycles.

• Earthquakes and Structures
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• v.12 no.5
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• pp.515-527
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• 2017

Recently, Friction dampers become popular due to the desirable performance in the energy dissipation of lateral loads. A lot of research which has been conducted on these dampers results in developing friction dampers with low sensitivity to the number of cycles and temperature increases. Friction dampers impose high residual drifts to the buildings because of low post-yield stiffness of the damper which results from increasing lateral displacement and period of buildings. This issue can be more critical under strong aftershocks which results in increasing of structural damages. In this paper, in addition to the assessment of aftershock on steel buildings equipped with friction dampers, methods for controlling residual drifts and decreasing the costs of retrofitting are investigated. Utilizing rigid connections as a lateral dual system and activating lateral stiffness of gravity columns by adding elastic braces are as an example of effective methods investigated in this research. The results of nonlinear time history analyses on the low to medium rise steel frames equipped with friction dampers illustrate a rise in residual drifts as the result of aftershocks. In addition, the results show that different slip loads of friction damper can affect the residual drifts. Furthermore, elastic stories in comparison to rigid connections can reduce residual drifts of buildings in an effective fashion, when most slip loads of friction dampers are considered.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1267-1277
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• 2016

Currently, enzymatic synthesis of lactulose, a synthetic prebiotic disaccharide, is commonly performed with glycosyl hydrolases. In this work, a new type of lactulose-producing biocatalyst was developed by displaying β-galactosidase from Bacillus stearothermophilus IAM11001 (Bs-β-Gal) on the surface of Bacillus subtilis 168 spores. Localization of β-Gal on the spore surface as a fusion to CotX was verified by western blot analysis, immunofluorescence microscopy, and flow cytometry. The optimum pH and temperature for the resulting spore-displayed β-Gal was 6.0 and 75℃, respectively. Under optimal conditions, it showed maximum activity of 0.42 U/mg spores (dry weight). Moreover, the spore-displayed CotX-β-Gal was employed as a whole cell biocatalyst to produce lactulose, yielding 8.8 g/l from 200 g/l lactose and 100 g/l fructose. Reusability tests showed that the spore-displayed CotX-β-Gal retained around 30.3% of its initial activity after eight successive conversion cycles. These results suggest that the CotX-mediated spore-displayed β-Gal may provide a promising strategy for lactulose production.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1234-1241
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• 2016

Methanol is a versatile compound that can be biologically synthesized from methane (CH₄) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH₄ as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH₄, pH 7, 150 rpm, 30℃, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.325-331
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• 2010

Rhodococcus erythropolis amidase was expressed in Escherichia coli cells. The crude amidase in the cell-free extract was immobilized using the cross-linked enzyme aggregate (CLEA) method. The crude amidase was mixed with bovine serum albumin and then precipitated with ammonium sulfate. The resultant precipitant was subsequently cross-linked with glutaraldehyde. Scanning electron microscopy revealed that this co-CLEA had a ball-like shape with a diameter of approximately $1\;{\mu}m$. This co-CLEA evidenced hydrolytic activity toward a variety of amide substrates. The amidase co-CLEA evidenced an optimum temperature of $60^{\circ}C$ and an optimum pH of 8.0, results that were similar to those of the soluble amidase. The reaction stability of the co-CLEA was increased. That is, it was stable up to $50^{\circ}C$ and in a pH range of 5.0-12.0. Additionally, the co-CLEA could be recovered by centrifugation, and retained 96% activity after 3 repeated cycles. This amidase co-CLEA may prove useful as a substitute for soluble amidase as a biocatalyst in the pharmaceutical and chemical industries.

• 한국초전도학회:학술대회논문집
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• v.9
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• pp.7-11
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• 1999

The recent progress in new cooling techniques of the high Tc superconductor(HTS) systems is reported and discussed with some practical examples. At the beginning stage of the HTS development in research laboratories, liquid nitrogen(LN$_2$) is the standard medium for an effective cooling. The success of HTS in many different application areas, however, has required a variety of need in the cooling temperature and the cooling capacity with specific design restrictions. While the utilization of alternative liquid cryogens such as liquid neon (LNe) or liquid hydrogen (LH$_2$) has been tired in some of them, even solid cryogens such as solid nitrogen (SN$_2$) or solid hydrogen (SH$_2$) may be another option in special applications. The gaseous helium cooled by a cryogenic refrigerator has also been a good candidate in many cases. One of the best cooling methods for the HTS is the direct conduction-cooling by a closed-cycle refrigerator with no cryogen at all. The refrigeration may be based on Joul-Thomson, Brayton, Stirling, Gifford-McMahon, or pulse tube cycles. The pros and cons of the newly proposed cooling methods are described and some significant design issues are presented.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.7
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• pp.612-617
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• 2011

An experimental study was carried out for the Elbrodur-NIB(copper alloy) at room temperature under stress-controlled uniaxial fatigue loading with and without mean stress. As a result, the effects of stress amplitude, mean stress and stress rate on ratcheting behavior were investigated. The ratcheting strain increased with increasing stress amplitude for a given mean stress, and with mean stress for a given stress amplitude. But, the ratcheting strain decreased as the stress rate increased. The three mean stress models were investigated and the mean stress models of Smith-Watson-Topper and Walker yielded good correlation of fatigue lives in the life range of $10^2-10^5$cycles.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2007.04a
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• pp.37-47
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• 2007

Factor : Structure Metal pad & SMO size Board level TC test : - Large SMO size better Board level Drop test : - Large SMO size better Factor : Structure Substrate thickness Board level TC test : - Thick substrate better Board level Drop test : - Substrate thickness is not a significant factor for drop test Factor : Material Solder alloy Board level TC test : - Not so big differences over Pb-free solder and NiAu, OSP finish Board level Drop test : - Ni/Au+SAC105, CuOSP+LF35 are better Factor : Material Pad finish Board level TC test : - NiAu/NiAu is best Board livel Drop test : - CuOSP is best Factor : Material Underfill Board level TC test - Several underfills (reworkable) are passed TCG x500 cycles Board level Drop test : - Underfill lots have better performance than non-underfill lots Factor : Process Multiple reflow Board level TC test : - Multiple reflow is not a significant actor for TC test Board level Drop test : N/A Factor : Process Peak temp Board level TC test : - Higher peak temperature is worse than STD Board level Drop test : N/A Factor : Process Stack method Board level TC test : - No big difference between pre-stack and SMT stack Board level Drop test : - Flux dipping is better than paste dipping but failure rate is more faster

• Transactions of the Korean hydrogen and new energy society
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• v.18 no.4
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• pp.382-390
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• 2007

To check the feasibility of SMART(Steam Methane Advanced Reforming Technology) system, an experimental investigation was performed. A fluidized bed reactor of diameter 0.052m was operated cyclically up to 10th cycle, alternating between reforming and regeneration conditions. FCR-4 catalyst was used as the reforming catalyst and calcined limestone(domestic, from Danyang) was used as the $CO_2$ absorbent. Hydrogen concentration of 98.2% on a dry basis was reached at $650^{\circ}C$ for the first cycle. This value is much higher than $H_2$ concentration of 73.6% in the reformer of conventional SMR (steam methane reforming) condition. The hydrogen concentration decreased because the $CO_2$ capture capacity decreased as the number of cycles increased. However, the average hydrogen concentration at 10th cycle was 82.5% and this value is also higher than that of SMR. Based on these results, we could conclude that the SMART system can replace SMR system to generate pure hydrogen without HTS (high tempeature shift), LTS (low temperature shift) and $CO_2$ separation process.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.25-28
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• 2008

Generally, reinforced concrete structures exposed to the outside temperature are affected by freezing and thawing process during winter and early spring. These freezing and thawing process can lead to the reduction in durability of concrete as cracking or surface spalling. This paper is to study the hysteretic behavior of RC beams exposed to freezing and thawing under cyclic loadings. To compare the difference in hysteretic behavior of RC Beams, limited tests were conducted under different types of damage and freezing and thawing cycles. For this purpose, six specimens were tested. It is thought that experimental results will be used as basic data to evaluate hysteretic behavior of RC beams exposed to freezing and thawing.