• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.272-272
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• 2022

Water and phosphorus (P) fertilizer are two of the most critical inputs in rice cultivation. Under climate change scenarios and urbanization, irrigation and fertilizer are becoming limiting factors often leading to significant decrease in yield whenever supplied scarcely. It has been shown that the Pup1 QTL confers tolerance to P starvation and improved early-stage root vigor in indica rice grown in the tropics. However, whether the QTL works in japonica rice genetic background grown in temperate regions remains to be elucidated. Here, we have introgressed the Pup1 QTL into three temperate rice varieties MS11, TR22183, and Dasan using marker-assisted backcrossing and next generation sequencing. The selected lines all harbored the full Pup1 QTL with recurrent parent genome recovery rates ranging from 66.5% to 99.8%. Evaluation of the introgression rice lines grown in South Korea under low inputs of P and water revealed early vegetative growth advantage relative to that of the recurrent parents. Under rainfed condition, Pup1 introgression lines had yield advantage ranging from 7.2 to 19.9% and 24 to 26% in P non-supplied soil and P-supplied soil, respectively compared to that of the recurrent parents suggesting that Pup1 confers enhanced yield under low P and water inputs in temperate rice genetic background grown in temperate climate. In terms of early vegetative growth, temperate Pup1 introgression lines showed a similar trend on the extent to which Pup1 promotes yield advantage in temperate rice in comparison with indica control Pup1 introgression line IR64-Pup1.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.214-220
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• 1999

Rice production in Korea has markedly increased during the last two decades due to newly developed high yielding varieties and improved production technology. This experiment was conducted to determine the potential of tropical japonica germplasm in enhancing the yield of temperate japonica. The yield performance of two tropical japonicas (IR 65597-29-3-2 and IR66154-52-1-2) and one temperate japonica (Dongjinbyeo) was compared at different plant densities and nitrogen levels under Korean environmental conditions. Although tropical japonicas showed low tillering habit and large panicles, they had similar leaf area index and dry weight at heading stage to Dongjinbyeo of the high tillering type indicating that there was not much difference between tropical and temperate japonica in terms of biomass production. The highest milled rice yield of 6.15 t/ha was obtained from Dongjinbyeo at a high nitrogen level with less planting density (220 kg N/ha and 30 $\times$ 15 cm). However, those of the two tropical japonicas were 5.36 t/ha at the condition of 165 kg N/ha and 30 x 10 cm planting density and 5.06 t/ha at the condition of 165kgN/ha and 15 x 15 cm planting density, respectively. Ripened grain of tropical japonicas ranged from 65 to 87%, while that Dongjinbyeo ranged from 82 to 97% under Korean conditions.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.214-214
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• 2022

Soluble starch synthases (SSs) elongate α-glucans from ADP-Glc to the glucan nonreducing ends and play a critical role in synthesizing resistant starch in the rice. A total of 10 SSs isoforms were reported in rice, including granules-bound starch synthase I (GBSSI), GBSSII, starch synthase I (SSI), SSIIa (SSII-3), SSIIb (SSII-2), SSIIc (SSII-1), SSIIIa (SSIII-2), SSIIIb (SSIII-1), SSIVa (SSIV-1), and SSIVb (SSIV-2). SSIII proteins are involved in forming the B chain and elongating cluster filling chains in amylopectin metabolism. The functions of SSIIIb (SSIII-1) are less clear as compared to SSs. Here, we sought to shed light on the genetic diversity profiling of the SSIII-1 gene in 374 rice accessions composed of 54 wild-type accessions and 320 bred cultivars (temperate japonica, indica, tropical japonica, aus, aromatic, and admixture). In total, 17 haplotypes were identified in the SSIII-1 coding region of 320 bred cultivars, while 44 haplotypes were detected from 54 wild-type accessions. The genetic diversity indices revealed the most negative Tajima's D value in the temperate-japonica, followed by the wild type, while Tajima's D values in other ecotypes were positive, indicating balancing selection. Nucleotide diversity in the SSIII-1 region was highest in the wild group (0.0047) while lowest in temperate-japonica. Lower nucleotide diversity in the temperate-japonica is evidenced by the negative Tajima's D and suggested purifying selection. The fixation index (F_ST) revealed a very high level of gene flow (low F_ST) between the tropical-japonica and admixture groups (F_ST=-0.21) followed by admixture and wild groups (-0.04), indica and admixture groups (0.02), while low gene flow with higher F_ST estimates between the temperate-japonica and aus groups (0.72), tropical-japonica and aromatic groups (0.71), and temperate-japonica and admixture groups (0.52). Taken together, our study offers insights into haplotype diversity and evolutionary fingerprints of SSIII-1. It provides genomic information to increase the resistant starch content of cooked rice.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.212-212
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• 2022

Soluble starch synthase (SS) IV-2 is one of the starch synthase gene family members and responsible for starch chain elongation interacting with other rice eating and cooking quality controlling genes (e.g., AGPlar and PUL). SSIV-2 is mainly expressed in leaves, especially at grain-filling stage and its alleles can significantly affect rice quality. Here, we investigated the genetic diversity and population structure analyses of SSIV-2 gene by using 374 rice accessions. This rice set was grouped into 320 cultivated bred (subsequently classified into temperate japonica, indica, tropical japonica, aus, aromatic and admixture) and 54 wild rice. Haplotyping of cultivated rice accessions provided a total of 7 haplotypes, and only three haplotypes are functional indicating four substituted SNPs in two exons of chromosome 5: T/A and G/T in exon 4, and C/G and G/A in exon 13. Including the wild, a highest diverse group (0.0041), nucleotide diversity analysis showed temperate japonica (0.0001) had a lowest diversity value indicating the origin information of this gene evolution. Higher and positive Tajima5s D value of indica (1.9755) indicate a selective signature under balancing selection while temperate japonica (-0.9018) was in lowest Tajima's D value due to a recent selective sweep by positive selection. We found the most diverse genetic components of the wild in PCA but shared in some portion with other cultivated groups. Fixation index (F_ST-values) and phylogenetic analysis indicate a closer relationship of the wild with indica (F_ST=0.256) than to its association to both of temperate japonica (F_ST=0.589). Structure analysis shows a clear separation of cultivated subpopulations at every K value, but genetic components were admixed within the wild illustrating the same genetic background with japonica and indica in some proportion.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.171-171
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• 2022

Effect of temperature during the period of 10 days from transplanting (10 DFT) on the growth and development of rice plants was investigated by transplanting semi-adult seedlings six times from 5 April to 15 May at 10-day interval in the field conditions of 2020 and 2021, with aims to investigate the critical temperature for early transplanting of temperate rice. In the two experimental years, mean temperature for 10 DFT appeared 9.1, 10.5, 11.6, 13.8, 13.9, 16.2, 16.4, 16.7, 17.1, 17.8℃ depending on the transplanting date. Mean temperature of 9.1℃ and 10.5℃ for 10 DFT appeared in the April 5 and April 15 transplants in 2020 showed negative or no effect on the increase of rice growth and acceleration of heading date when compared to those of right after transplanting treatments in the same year. Mean temperature of 11.6℃ for 10 DFT appeared in the April 5 transplant in 2021 demonstrated greater biomass from early to heading stage but the same heading date compared to April 15 transplant, indicating that 11.6℃ for 10 DFT had a positive effect on rice growth but no effect on advanced heading. Both more biomass and advanced heading stage were observed when the mean temperature for 10 DFT was 13.8℃ or higher, compared to those of right after transplanting treatments. These findings indicate that effective 10-DFT mean temperature for rice growth exists between 10.5 and 11.6℃, and that for rice development in terms of heading stage lies between 11.6 and 13.8℃ in natural condition. Further field and indoor studies are suggested to narrow down the critical temperature for early transplanting of temperate rice, which will enable to maximize the crop period in high altitude regions with low temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.120-130
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• 2018

Improvement in rice grain yield has been approached by means of genetic amendment, cultural management, and environmental adaptation. Subjecting the plant during the grain filling period to an appropriate environment plays a key role in achieving a high grain yield in temperate rice. Field experiments were conducted for two consecutive years with two planting times to assess the relations among grain filling traits, loss of leaf activity during the ripening period, and the grain yield of temperate japonica rice with wide environmental variation. Higher grain yields were attained in 2017 than in 2016 and with late planting than with early planting. The high grain yield accompanied a comparatively lesser increase in grain weight at the early filling stage but more gain in grain weight occurred during the late filling stage. Final grain weight correlated positively with grain filling duration but negatively with grain filling rate. Extended grain filling duration was associated with higher cumulative temperature and cumulative solar radiation for an effective grain filling period. The reduction in SPAD value ${\times}$ leaf dry weight from heading to harvest significantly correlated with final grain dry weight in a positive manner. No significant relation was found between grain filling duration and the decrease in SPAD value ${\times}$ leaf dry weight during the grain filling period. The results suggest that grain filling duration and loss of leaf activity during ripening independently contribute to environmentally induced yield improvement in temperate japonica rice.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.220-220
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• 2022

Disproportionating Enzyme 1 (DPE1) is an a-1,4-D-glucanotransferase that cleavages the a-1,4-glucosidic bonds and transfers glucosyl groups. In rice endosperm, it participates in starch synthesis by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Here, we investigated the haplotype variations and evolutionary indices (e.g., genetic diversity and population structure) for the DPE1 gene in 374 rice accessions representing seven subgroups (wild, indica, temperate japonica, tropical japonica, aus, aromatic, and admixture). Variant calling analysis of DPE1 coding regions leads to the identification of six functional haplotypes representing/occupying 8 nonsynonymous SNPs. Nucleotide diversity analysis revealed the highest pi-value in wild group (0.0556) compared to other cultivated groups, of which temperate japonica showed the most reduction of genetic diversity value (0.003). A significant positive Tajima's D value (1.6330) of admixture highlights sudden population contraction under balancing selection, while temperate japonica with the lowest Tajima's D value (-1.3523) showed a selection signature of DPE1 domestication which might be the cause of excess of rare alleles. Moreover, these two subpopulations exhibits a greater differentiation (F_ST=0.0148), indicating a higher genetic diversity. Our findings on functional DPE1 haplotypes will be useful in future breeding programs, and the evolutionary indices can also be applicable in functional studies of the DPE1 gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.135-140
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• 1998

Diallel analysis was conducted with FI's derived from crossing in all combinations without reciprocals among six rice varieties; three tropical japonica and three temperate japonica varieties, with different traits associated with panicle and flag leaf. Epistasis was observed in the number of primary branches (PB) per panicle and of spikelets per panicle, while flag leaf length, flag leaf color, PB length and neck node thickness were explained with the additive-dominance model. The estimated genetic mode of flag leaf length and PB length was a positive complete dominance model with high heritability, and that of flag leaf color and neck node thickness was an incomplete dominance model. In particular, tropical japonica varieties with low-tillering and heavy-panicle appear to have higher number of dominant genes for flag leaf length and PB length than temperate japonica varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.112-121
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• 2007

Grain filling is a crucial factor that determines grain yield in crops since it is the final process directly associated with crops' yield performance. Grain filling process can be characterized by the interaction of rate and duration of grain filling. This study was conducted, using 16 temperate japonica rice genotypes, with aims to (1) seek variations in grain filling duration and rate on area basis, (2) compare the contribution of grain filling duration and rate to grain yield, and (3) examine the influence of temperature and solar radiation for effective grain filling on grain yield in relation to grain filling duration and rate. Grain filling rate and duration exhibited highly significant variations in the ranges of $20.7{\sim}46.3\;g\;m^{-2}d^{-1}\;and\;11.2{\sim}35.5$ days, respectively, depending on rice genotypes. Grain yield on unit area basis was associated positively with grain filling duration but negatively with grain filling rate. Grain filling rate and duration were negatively correlated with each other. Final grain weight increased linearly with the rise in both cumulative mean temperature and cumulative solar radiation for effective grain filling. Higher cumulative mean temperature and cumulative solar radiation for effective grain filling were the results of longer grain filling duration, but not necessarily higher daily mean temperature and daily solar radiation for effective grain filling. Grain filling rate demonstrated an increasing tendency with the rise in daily mean temperature for effective grain filling but their relationship was not obviously clear. It was concluded that grain filling duration, which influenced cumulative mean temperature and cumulative solar radiation for effective grain filling, was the main factor that determined grain yield on unit area basis in temperate Japonica rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.3
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• pp.186-190
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• 2005

This experiment was conducted to collect the basic information on the varietal diversity in amylogram properties of 3 different rice sub-species under tropical conditions in IRRI 2001 wet season based upon correlation and principal component analysis. The peak viscosity and breakdown property of Tongil type, i.e.; temperate Indica species showed higher similarity with Japonica type species rather than typical Indica and tropical japonica types. The amylogram properties such as final viscosity, pasting consistency and setback of Tongil type varieties were lower than those of typical Indica and tropical japonica types. The peak viscosity showed positive correlation with trough, while the breakdown showed negative correlation with setback in all tested 3 rice subspecies. The first principal component was applicable to increase the gelatinization temperature, final viscosity, pasting consistency and setback, and applicable to decrease the peak viscosity and breakdown. Varietal classification by the principal component score of each pedigree could be applied to the interpretation of the community by the scatter diagram for the amylogram properties to the different sub-species of rice at IRRI conditions.