• 한국균학회지
• /
• 제46권4호
• /
• pp.467-477
• /
• 2018

탄저병균인 Colletotrichum circinans는 세계적으로 양파에 심각한 피해를 주는 병원균이다. 본 연구에서는 일반 PCR방법과 real-time PCR방법으로 C. circinan를 정확하면서도 쉽고 빠르게 검출이 가능한 특이 마커를 개발하였다. $tef-1{\alpha}$ 유전자와 ${\beta}-tubulin$ 유전자를 분석하여 C. circinan를 특이적으로 검출할 수 있는 cirTef-F/cirTef-R set와 cirTu-F/cirTu-R set를 제작하였다. 일반 PCR 방법으로 cirTef-F/cirTef-Rset는 100pg, cirTu-F/cirTu-Rset는 1ng까지 검출이 가능하였고 real-time PCR 방법으로는 각각 10 pg, 100 pg까지 검출이 가능하였다. C. circinans에 인공적으로 감염된 양파 종자에서도 cirTef-F/cirTef-Rset를 사용하여 일반 PCR방법과 real-time PCR 방법 모두 C. circinans검출이 가능하였다. 본 연구에서 개발한 C. circinans 특이 검출마커는 수출입 되는 채소 및 종자에서 빠르고 정확하게 탄저병균인 C. circinans를 검출하는데 사용될 수 있을 것이다.

• Advances in pediatric surgery
• /
• 제17권1호
• /
• pp.81-87
• /
• 2011

This study was aimed to evaluate associated congenital anomalies in the patients with esophageal atresia with tracheoesophageal fistula (EA/TEF). Forty-two neonates with the diagnosis of EA/TEF treated over a 10 year period in a single institution were included in this study. The demography of EA/TEF was analyzed. Major associated anomalies including vertebral, anal, cardiac, renal, limb, neurologic and chromosome were reviewed and categorized. Males were slightly more dominant than females (1.47:1) and all patients had Gross type C EA/TEF. Only 19 % of the patients had solitary EA/TEF without associated anomalies. Cardiac anomalies were the most common associated congenital anomaly in patients with EA/TEF (73.8 %). But 47.6 % were cured spontaneously or did not affect patients' life. Atrial septal defect (ASD) was the most common cardiac anomaly followed by patent ductus arterious (PDA) and ventricular septal defect (VSD). Among gastrointestinal anomalies (23.8 %), anorectal malformations were the most frequent, 70 % Vertebral and limb abnormalities accounted for 11.9 % and urogenital malformations 9.5 % of the anomalies in patients with EA/TEF. VACTERL associated anomalies were 23.8 % and 1.8% had full VACTERL. Almost 12 % of EA/TEF had neurologic anomalies. Patients with EA/TEF require preoperative evaluation including neurologic evaluation to detect anomalies not related to VACTERL. Though associated cardiac anomaly occurred in 73.8 % of patients in our study, only 21.42 % needed surgical correction. The authors suggesrs further studies with large numbers of patients with EA/TEF.

• Journal of Chest Surgery
• /
• 제54권3호
• /
• pp.206-213
• /
• 2021

Background: Tracheoesophageal fistula (TEFs) is a rare condition that requires complex surgical treatment. We analyzed the surgical outcomes of TEF reported in the literature and at Pusan National University Yangsan Hospital using standardized techniques. Methods: This retrospective study included 8 patients diagnosed with acquired benign TEF between March 2010 and December 2019. The surgical method was determined based on the size of the fistula observed within the endoscope. Results: TEF occurred in 7 patients (87.5%) after intubation or tracheostomy and in 1 patient (12.5%) after esophageal surgery due to conduit necrosis. For tracheal management, 5 and 2 patients underwent tracheal resection and end-to-end anastomosis and primary repair, respectively. The median length of resection was 2.5 cm (range, 1.3-3.4 cm). For esophageal management, 6 patients underwent primary repair and 1 patient underwent esophageal diversion. One patient underwent TEF division with a stapler. Interposition of a muscle flap was performed in 2 patients. TEF recurrence, esophageal stenosis, and dehiscence or granulation occurred in 1, 1, and 2 patients, respectively. A long-term tracheostomy tube or T-tube was used in 2 patients for >2 months. Conclusion: Although TEF surgery is complex and challenging, good results can be achieved if surgical standards are established and experience is accumulated.

• Journal of Chest Surgery
• /
• 제27권10호
• /
• pp.888-892
• /
• 1994

Acquired, traumatic tracheoesophageal fistula [TEF] is rare and difficult problem to manage. This 55 years old man met with a roller accident of a tractor. During accident, he received a penetrating injury on the left upper sternal border. At local clinic, he received closed thoracotomy drainage [CTD]for relief of pneumothorax[left]. Three days after CTD, he complained abdominal pain and hematemesis. The endoscopy revealed large ulcer at the stomach, so he received subtotal gastrectomy. On 10th day post subtotal gastrectomy, he developed aspiration and coughing from a TEF. The esophagogram showed large TEF at the mid-trachea level. So he transfered to our hospital for operation. This patient was operated on for late TEF three weeks after injury. We have used absorble 4-0 Vicryl sutures to repair trachea. We repair all esophageal injuries with two layers of nonabsorbable silk suture. Where suture line on the esophagus, the strap muscle was interposed for reinforcement. And for feeding, the feeding jejunostomy was performed. Postoperatively the osteomyelitis of the manubrium site was developed, so on the 30th postoperative day, an ostectomy of manubrium, both clavicle and fight 1st, 2nd ribs, and the pectoralis major musculo-cutaneous flap coverage were performed.

• 한국멀티미디어학회논문지
• /
• 제25권1호
• /
• pp.82-91
• /
• 2022

In this paper, time series prediction method of photovoltaic power is introduced using seasonal autoregressive integrated moving average (SARIMA). In order to obtain the best fitting model by a time series method in the absence of an environmental sensor, this research was used data below 50% of cloud cover. Three samples were extracted by time intervals from the raw data. After that, the best fitting models were derived from mean absolute percentage error (MAPE) with the minimum akaike information criterion (AIC) or beysian information criterion (BIC). They are SARIMA (1,0,0)(0,2,2)14, SARIMA (1,0,0)(0,2,2)28, SARIMA (2,0,3)(1,2,2)55. Generally parameter of model derived from BIC was lower than AIC. SARIMA (2,0,3)(1,2,2)55, unlike other models, was drawn by AIC. And the performance of models obtained by SARIMA was compared. MAPE value was affected by the seasonal period of the sample. It is estimated that long seasonal period samples include atmosphere irregularity. Consequently using 1 hour or 30 minutes interval sample is able to be helpful for prediction accuracy improvement.

• Advances in pediatric surgery
• /
• 제2권1호
• /
• pp.68-71
• /
• 1996

Esophageal atresia(EA) with a double tracheoesophageal fistula(TEF) is rare. It accounts for only 0.7% of all cases of EA and TEF. A male newborn weighing 2860g was born by normal vaginal delivery at 41 weeks' gestation to a 27-year-old mother who had a normal pregnancy. But immediately developed recurrent choking and respiratory distress. Feeding tube was inserted and chest X ray showed the feeding tube coiled in the proximal pouch(T-2 level). With a preoperative diagnosis of EA and distal TEF, the thoracotomy was performed on the third day of life. At thoracotomy, TEF was proved to be a double fistula. Both fistulas were divided and an esophageal anastomosis was performed. The postoperative course was uncomplicated until the eighth postoperative day when a minor anastomotic leak. The patient was discharged at 103 days of age.

• Current Research on Agriculture and Life Sciences
• /
• 제31권4호
• /
• pp.245-249
• /
• 2013

In 2008, leaf blight symptoms were observed on several Chinese chive farms in Sangju. The Pathogenicity of the isolate was confirmed by artificial inoculation, where the pathogen exhibited a strong pathogenicity toward healthy plants. Morphological classification identified the isolate as from the Fusarium genus. For further analysis, PCR and phylogenetic classification were performed with ITS region and 28S rRNA gene which are commonly used for fungal identification. However, the results provided a poor resolution. To solve this problem, we analyzed translation elongation factor 1-alpha (TEF-$1{\alpha}$) gene. The analyzed results using TEF-$1{\alpha}$ gene indicated that the isolate was F. proliferatum. Therefore, it is assumed that TEF-$1{\alpha}$ gene is important when Fusarium sp. was identified using molecular classification method.

• 대한기관식도과학회지
• /
• 제19권1호
• /
• pp.25-27
• /
• 2013

A 47-year-old man was referred for TEF. He underwent tracheostomy three months ago to maintain prolonged ventilator care. Computed tomography (CT) scan and fiberoptic examination showed bilateral vocal cord palsy with median fixation and about 2 inch sized long segmental tracheoesophageal fistula (TEF) tract along the necrotic cricoid and tracheal cartilages. Narrow field total laryngectomy was performed to remove devitalized cartilages and mucosa, and repair TEF. He discharged without complication except mild stenotic change of tracheal fenestration 19 days later.

• 한국균학회지
• /
• 제46권2호
• /
• pp.186-192
• /
• 2018

PCR 기술을 이용하여 고추와 토마토의 탄저병을 일으키는 Colletotrichum coccodes 균을 빠르고 정확하게 검출하는 방법을 개발하였다. Colletotrichum 13종 22개 균주로 부터 translation elongation factor 1 alpha 유전자를 분석하여 C. coccodes에 특이적인 coccoTef-F/cocco Tef-R primer set를 제작하였다. 제작된 primer를 사용한 결과 일반 PCR 방법으로 10 ng, real-time PCR 방법으로는 10 pg 수준에서 C. coccodes가 특이적으로 검출이 가능하였다. 인공적으로 C. coccodes에 감염시킨 고추와 토마토 종자에서도 일반 PCR 방법과 real-time PCR 방법 모두 C. coccodes검출이 가능하였다. 본 연구에서 개발한 PCR 방법은 수출입되는 종자에서 신속하고 정확하게 탄저병균 C. coccodes를 특이적으로 검출하는데 활용될 수 있을 것이다.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2007년도 제38회 하계학술대회
• /
• pp.449-450
• /
• 2007

본 논문에서는 과도안정도 문제 발생 시, 외란이 계통에 미치는 영향을 최소화할 수 있는 효과적인 제어 방법을 다룬다. 전력계통의 과도안정도는 외란에 의해 야기되는데, 이 외란의 영향을 받아 동기성을 잃고 가속하는 발전기들이 과도에너지를 계통에 유입시킨다. 따라서 가속하는 발전기들 중 일부를 제거하여 계통으로 유입되는 과도에너지를 줄이고 계통을 새로운 정상상태로 만들고자하는 것이 본 논문의 주된 내용이다. TEF 프로그램을 통하여 발전기 제거 시간과 계통의 안정성과의 관계를 다루고, 이를 PSS/E를 이용하여 검증한다. 실제 계통에서의 선로제거나 발전기 또는 부하 탈락은 0.1초로 가정하여 사례연구를 실행하였으며, 계통사고는 삼상 단락 사고로 하였다.