• The Korean Journal of Physiology and Pharmacology
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• v.24 no.3
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• pp.233-240
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• 2020

Autophagy regulators are often effective as potential cancer therapeutic agents. Here, we investigated paclitaxel sensitivity in cells with knockout (KO) of ATG5 gene. The ATG5 KO in multidrug resistant v-Ha-ras-transformed NIH 3T3 cells (Ras-NIH 3T3/Mdr) was generated using the CRISPR/Cas9 technology. The qPCR and LC3 immunoblot confirmed knockout of the gene and protein of ATG5, respectively. The ATG5 KO restored the sensitivity of Ras-NIH 3T3/Mdr cells to paclitaxel. Interestingly, ATG5 overexpression restored autophagy function in ATG5 KO cells, but failed to rescue paclitaxel resistance. These results raise the possibility that low level of resistance to paclitaxel in ATG5 KO cells may be related to other roles of ATG5 independent of its function in autophagy. The ATG5 KO significantly induced a G₂/M arrest in cell cycle progression. Additionally, ATG5 KO caused necrosis of a high proportion of cells after paclitaxel treatment. These data suggest that the difference in sensitivity to paclitaxel between ATG5 KO and their parental MDR cells may result from the disparity in the proportions of necrotic cells in both populations. Thus, our results demonstrate that the ATG5 KO in paclitaxel resistant cells leads to a marked G₂/M arrest and sensitizes cells to paclitaxel-induced necrosis.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.183-183
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• 2023

Smart water cities (SWC) are urban municipalities that utilizes modern innovations in managing and preserving the urban water cycle in the city; with the purpose of securing sustainability and improving the quality of life of the urban population. Understanding the different urban water characteristics and management strategies of cities situate a baseline in the development of evaluation scheme in determining whether the city is smart and sustainable. This research herein aims to develop measurements and evaluation for SWC Key Performance Indicators (KPIs), and set up a unified global standard and certification scheme. The assessment for SWC is performed in technical, as well as governance and prospective aspects. KPI measurements under Technical Pillar assess the cities' use of technologies in providing sufficient water supply, monitoring water quality, strengthening disaster resilience, minimizing hazard vulnerability, and maintaining and protecting the urban water ecosystem. Governance and Prospective Pillar on the other hand, evaluates the social, economic and administrative systems set in place to manage the water resources, delivering water services to different levels of society. The performance assessment is composed of a variety of procedures performed in a quantitative and qualitative manner, such as computations through established equations, interviews with authorities in charge, field survey inspections, etc. The developed SWC KPI measurements are used to evaluate the urban water management practices for Busan Eco Delta city, a Semulmeori waterfront area in Gangseo district, Busan. The evaluation and scoring process was presented and established, serving as the basis for the application of the smart water city certification all over the world. The established guideline will be used to analyze future cities, providing integrated and comprehensive information on the status of their urban water cycle, gathering new techniques and proposing solutions for smarter measures.

• ALGAE
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• v.26 no.1
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• pp.3-20
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• 2011

The physiology of the unicellular green alga Dunaliella salina in response to abiotic stress has been studied for several decades. Early D. salina research focused on its remarkable salinity tolerance and ability, upon exposure to various abiotic stresses, to accumulate high concentrations of $\beta$-carotene and other carotenoid pigments valued highly as nutraceuticals. The simple life cycle and growth requirements of D. salina make this organism one of the large-scale commercially exploited microalgae for natural carotenoids. Recent advances in genomics and proteomics now allow investigation of abiotic stress responses at the molecular level. Detailed knowledge of isoprenoid biosynthesis mechanisms and the development of molecular tools and techniques for D. salina will allow the improvement of physiological characteristics of algal strains and the use of transgenic algae in bioreactors. Here we review D. salina isoprenoid and carotenoid biosynthesis regulation, and also the biotechnological and genetic transformation procedures developed for this alga that set the stage for its future use as a production system.

• Journal of Agricultural Extension & Community Development
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• v.16 no.1
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• pp.99-124
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• 2009

The purposes of this study were (1) to highlight the importance of farm management (2) to develop a profitable farm management system through the combination of agricultural techniques and business competencies (3) to shift management attitudes and habits of the program participants from being passive and dependent toward being proactive and self-disciplined (4) to help cultivate one's own life through self-consciousness participation in educational activities and lectures of celebrities. This research was conducted involving 30 farms that participated in the business program. The program imparted knowledge on vision, marketing, business skills, and management strategies such as cross strengths, weaknesses, opportunities, threats (swot) analysis, product life cycle (plc), the 4p(product, price, place, promotion) of marketing, etc. The study recommends: (1) to encourage farms to enhance their business competencies; (2) to find out solutions for urgent management problems (3) to construct a system for leading farms (www.lfcenter.com) and on-line communities and (4) to implement a profitable business model for leading farms. It was noted that the farms were eager to participate in such business programs. In the future, this kind of program should be promoted in other areas so that more farms could enhance their business competencies.

• Proceedings of the Safety Management and Science Conference
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• 2013.11a
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• pp.393-400
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• 2013

Globally, smart phones have been rapidly distributed, which has led to changes in people's life cycle. Most people who are under 60 are supposed to use smart phones. Additionally, as the ratio of people who are interested in physical exercise is increasing, some applications for smart phones can manage dividual's exercise with the web servers. However, most of them can only check how much individual works out and cannot compare other's body type and life environment. Moreover, users cannot share their own data with others. This paper proposed the system which can resolve those kinds of problems through data mining techniques. The suggested model will have ability to figure out the relation between body type and the amount of exercise, find out if his work is proper from the result of classification and can pick out the features which is common to people who have similar body type and the amount of workout by applying data mining techiques. This model also will be able to recommend the proper amount of workout to each individual in order that they keep good health state efficiently.

• Journal of the Korean Society for Precision Engineering
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• v.14 no.12
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• pp.73-85
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• 1997

The rolling force and roll deformation behavior in the twin roll type strip continuous casting process has been computed to estimate the thermal charcteristics of a caster roll. To calculation of rolling force, the relationship between flow stress and strain for a roll material and casting alloy are assumed as a function of strain-rate and temperature because mechanical properties of a casting materials depends on tempera- ture. The three dimensional thermal dlastic-plastic analysis of a cooling roll has also been carried out to obtain a roll stress and plastic strain distributions with the commercial finite element analysis package of ANSYS. Temperature fields data of caster roll which are provided by authors were used to estimated of roll deformation. Roll life considering thermal cycle is calculated by using thermal elastic-plastic analysis results. Roll life is proposed as a terms of a roll revolution in the caster roll with and without fine failure model on the roll surface. To obtain of plastic strain distributions of caster roll, thermomechan- ical properties of roll sleeve with a copper alloy is obtained by uniaxial tensile test for variation of temperature.

• Journal of Cadastre & Land InformatiX
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• v.45 no.1
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• pp.207-224
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• 2015

The purpose of this study is to review the various valuation techniques of intangible assets. The value of intangible asset by the income approach can be measured as the present value of the economic benefit over the intangible asset's remaining useful life. The typical methods used in intangible asset economic income projections include extrapolation method, life cycle analyses, sensitivity analyses, simulation analyses, judgment method, and tabula rasa method. There are several methods available for estimating capitalization rates and discount rates for intangible asset, in which we have discussed market extraction method, capital asset pricing model, built-up method, discounted cash flow model, and weighted average cost of capital method. As the capitalization methods for intangible asset, relief-from-royalty method, excess earnings capitalization method, profit split method, residual from business enterprise method, postulated loss of income method and so on have been reviewed.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2006.11a
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• pp.123-130
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• 2006

Recently, Korean government promotes BTL(Build-Transfer-Lease) for their public construction project to apply private sector's experience and finance initiatives. Many of public buildings and civil infrastructure projects were planned to be delivered by BTL and it helps to continue public projects under tight budget conditions. Since the characteristics of BTL projects, creative ideas for the project function, performance and its LCC (Life Cycle Cost) become more important than usual public work project. This paper introduces the systematic "VE job Plan" application techniques for BTL project and shows the process. This study shows the application of "VE job Plan" for BTL project to help decision making and value improvement. Also, Project cost was estimated by LCC (Life Cycle Cost) analysis. Technical "FAST diagram"was developed and used to find major functions of the project. So, it helps to improve performance of functions and make creative ideas. FAST diagram also help to find relationships between functions and cost. A case study (Daegu Art Gallery BTL Project) was conducted using proposed "VE job Plan. Study demonstrates how value management helps save money and increase functional performance. Researcher found that project can save cost and improve performance of functions significantly by using appropriate VE analysis process at appropriate time.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.1
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• pp.52-59
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• 2015

Recently the dairy cow industry have faced environmental issues such as eutrophication, global warming, etc. An LCA was used to quantify the environmental impact of a dairy cow system and to identify key issues contributing to the impact. The system boundary crop cultivation for feeding dairy cow, feed production, rearing and manure management (cradle-to-gate). The functional unit was 1 kg of milk (fat protein corrected milk, FPCM) produced. Rearing and cultivation of feed crops stages in system boundary to the environmental impact of the domestic dairy cow system were dominant issues. Techniques such as suppression of enteric fermentation, improvement of the energy efficiency of farm equipment and apparatuses, management of leachate generated during the crop cultivation, and development of controling the loss of fertilizer during crop production would be necessary for the improvement of the environmental key issues of the dairy cow system.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.9
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• pp.1265-1272
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• 2010

In view of the frequent start-ups and load changes in the recent past, there is a need for fossil-fuel power plants to be more efficient and reliable for long-term operation. Under high-temperature and high-pressure conditions, severe creep and fatigue damages can occur in major plant facilities, particularly, turbines and boilers. For highly stable operation and better maintenance, various techniques that facilitate a systematic assessment of the service life of critical facilities have been developed. However, to date, in Korea, to evaluate the remaining life of major facilities of fossil power plant, qualitative or semiquantitative analyses are carried out without following any standard guidelines or procedure. In this study, a standard code for assessing the remaining life of major plant facilities is proposed. This code takes into account creep and fatigue damage, which are generally accepted as dominant causes of damage to facilities. KEPIC (Korea Electric Power Industry code) is scheduled to include this guideline in 2010.