• 동아시아식생활학회지
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• 제12권1호
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• pp.23-31
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• 2002

한약재를 기능성 식품에 이용하기 위하여 한약재의 생리활성 인자를 탐색할 목적으로 한약방에서 널리 이용되고 있는 황기(Astragalus membranaceus Bunge), 국화(Chrysanthemum morrifolium Ramat), 구기자(Lycium chinensis Miller), 감초(Glycyrrhiza uralensis Fischer), 당귀(Angelica gigas Nakai), 대추(Zizyphus jujuba Miller), 작약(Paeonia lactiflora Pallas), 천궁(Cnidium officinale Makino)을 물과 에탄올로 추출하여 항산화 활성을 DPPH법, SOD유사활성 측정법, thiocyanate법 및 TBARS법으로 측정하였다. 에탄을 추출물에 비하여 물 추출물의 페놀성 화합물의 함량이 높게 나타났으며, 구기자 물 추출물의 함량이 1.66%로 가장 높게 나타났다. 물 추출물 중 국화는 63.0% 그리고 에탄을 추출물 중 감초가 63.8%으로 BHT의 전자공여능(49.7%) 보다 높게 나타냈으며, SOD 유사활성은 물 추출물 중 황기에서 41.8%의 높은 활성능을 보였으며 에탄을 추출물 중 구기자에 38.2%의 높은 활성능을 보였다. 물 추출물 중 감초와 대추가 에탄을 추출물 중에는 당귀와 국화의 TBARS 값이 대조구에 비하여 5.0이상으로 항산화 활성이 BHT의 항산화 활성과 비슷하게 나타났으며, Thiocyanate법에의한 항산화 활성은 대조구와 비교하여 국화, 구기자, 감초, 작약의 물 추출물의 유도기간이 8일까지 연장되는 것을 볼 수 있으며 에탄을 추출물 중에는 감초와 국화의 유도기간이 8일가지 연장되었다. 이상의 결과에서 황기, 국화, 구기자, 감초, 당귀, 대추, 작약, 천궁의 물과 에탄을 추출물 중에는 in vitro 항산화 실험 계에서 항산화 활성을 나타내는 생리활성 성분이 있는 것으로 나타났다.

• 한국식품저장유통학회지
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• 제11권4호
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• pp.441-447
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• 2004

돼지고기와 쇠고기의 부위별 저장 중 발생하는 품질 변화와 부패시점을 확인하고자 돼지고기의 등심, 삼겹살, 전지와 쇠고기의 안심, 등심, 양지 부위를 냉장 저장 하면서 pH, VBN, TBARS, 전자코 측정 및 관능검사를 수행하였다. 돼지고기의 경우 삼겹살 부위가 등심, 전지와 비교하여 pH, VBN, TBARS에서 전반적으로 높은 결과를 나타냈고, 부패취를 확인하기 위해 실시한 전자코 분석에서는 저장 초기와 저장 말기의 주성분 분석 값이 뚜렷이 구분되었다. 부패시 점을 확인하기 위해 실시한 관능검사는 저장 4일 경과 후부터 변패가 시작되어 저장 기간이 6일 경과 후부터는 식용이 불가능한 상태에 이르렀다. 쇠고기의 경우 등심과 안심 부위가 양지와 비교하여 pH, VBN에서 현저한 차이를 보이며 큰 수치를 나타내었고, TBARS에서는 모든 부위가 저장 초기에는 비슷한 값을 보였으나 저장 기간이 경과함에 따라 등심부위의 값이 양지, 안심과 달리 급격히 증가하였다. 반면에 양지 부위는 모든 실험 항목에서 전반적으로 낮은 수치를 보였다. 전자코 분석에서는 돼지고기와 동일하게 저장초기와 저장 말기의 PCA에 있어서 뚜렷한 차이를 보였다.

• 한국축산식품학회지
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• 제22권3호
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• pp.222-227
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• 2002

진공포장한 한우육 우둔부위를 1$^{\circ}C$, 4$^{\circ}C$의 일반공기순환 냉장고와 1$^{\circ}C$, 4$^{\circ}C$의 냉수에 침지하여 3, 7, 10, 14일 동안 저장하였을 때, 처리구간의 물리적 육색 (CIE L*, a*, b*)은 크게 변화하지 않은 것으로 나타났다. 그러나 육색소 산화를 나타내는 MetMb의 함량은 처리구간에 따라 변화를 보여 대조구 (4$^{\circ}C$-공기순화)가 가장 빠른 증가를 나타내었으며, 저장 14일째 T2 (4$^{\circ}C$-냉수침지)구가 유의적으로 (p<0.05) 낮은 MetMb함량을 보였다. 한편 이런 결과는 지방산화와도 유사한 경향을 보였는데, 대조구 (4$^{\circ}C$-공기순환)의 TBARS 수치가 저장기간 동안 가장 빠른 증가를 나타낸 반면, T3(1$^{\circ}C$-냉수침지) 구의 TBARS는 크게 증가하지 않았다. 이러한 결과는 냉수침지 방법이 지방산화와 육색소 산화를 억제시킬 수 있다는 것을 의미한다. 포장감량 또는 공기순환 처리구(control, T1)가 냉수침지 처리구(T2, T3)에 비해 높게 나타났다. 총 육색소 함량은 전 저장기간 동안 처리구간에 유의적(p>0.05)인 차이가 없었다. 이상의 결과를 종합해 볼 때, 한우육을 진공포장하여 1$^{\circ}C$ 냉수에 침지시켜 숙성시키는 방법이 지방산화를 억제시키고 육색소의 안전성을 유지할 뿐만 아니라 포장감량도 줄일 수 있는 숙성법이라고 사료된다.

• 한국축산식품학회지
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• 제26권3호
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• pp.276-283
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• 2006

쑥 분말 3 %, 정어리유 4 %, 쑥 분말 3 %와 정어리유 4 %를 처리구에 따라 5주간 급여한 후 도계하여, 가슴살과 다리살을 냉장온도($4{\pm}1^{\circ}C$)에서 15일간 저장하면서 지방산 조성, TBARS가, 보수성, 육즙 손실의 변화를 조사하였다. 지방산은 근육의 부위 및 쑥과 어유의 급여 수준에 관계없이 oleic acid, palmitic acid, linoleic acid, stearic acid의 순으로 함량이 높았으나, 유의성은 없었다(p<0.05). 또한 T3 처리구에서는 palmitoleic acid와 EPA, DHA의 함량이 대조구보다 많았다. TBARS가는 저장기간이 경과함에 따라 전처리구에서 증가하였고(p<0.05), 전반적으로 다리살 부위가 가슴살부위의 TBARS가 보다 높은 경향이었다. 보수력은 저장기간이 경과함에 따라 가슴살과 다리살의 보수력은 증가하는 경향이었고(p<0.05), 처리구간에서는 서로 비슷하였으며, 전반적으로 가슴살이 다리살 부위보다 보수력이 높았다. 육즙 손실은 저장기간이 경과함에 따라 전처리구에서 점차 증가하였고(p<0.05), 처리구간에서는 T1구와 T3구가 상대적으로 육즙 손실이 적었다.

• 한국축산식품학회지
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• 제26권3호
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• pp.322-330
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• 2006

Sixteen semimembranous muscles were removed from sixteen left pig carcasses. They were cut into $7{\times}10{\times}2cm$ pieces and mixed randomly. Samples were assigned to four treatments: (T1) soy-based sauce; (T2) Kimchi-based sauce; (T3) pickled shrimp-based sauce; and (T4) onion-based sauce. Each sample was aged in a plastic box at $1^{\circ}C$ for 10 days, then vacuum packed and held at $1^{\circ}C$ for 28 days. The lightness and redness values of the aged pork were, in most cases, significantly increased on the surface and in the interior (p<0.05) by day 28 for all treatments, relative to day 1. The thiobarbituric acid reactive substances (TBARS) value significantly (p<0.05) increased for T1 and T4 from day 1 until day 14, but decreased after 14 days of storage (p<0.05). The TBARS value for T3 decreased with storage time (p<0.05), although there was no difference between 14 and 28 days. The total volatile basic nitrogen (VBN) content increased significantly with storage time (p<0.05) for all treatments, with the exception of T2. Total plate counts (TPC) increased significantly (p<0.05) with increasing storage time for all treatments. On day 1, T2 had the highest TPC value (p<0.05), while T4 was lowest (p<0.05). On 28 day, T2 had the lowest TPC value (p<0.05), while T3 was highest (p<0.05). E. coli levels showed a significant (p<0.05) decrease with increased storage for T1, T2 and T4. These results indicate that T2 was move effective at inhibiting the growth of E. coli than the other pork samples. The levels of Lactobacillus spp. increased with storage time for all samples. These results suggest that traditional Korean ingredients could be utilized to extend the shelf-life of aged pork during storage.

• Preventive Nutrition and Food Science
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• 제8권4호
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• pp.383-389
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• 2003

This study was conducted to investigate the antioxidative effects of Paeonia lactiftora Pall. (PL) seed extracts and Forsythia viridissima Lindl. (FVL) extracts on the antioxidative defense system and lipid peroxidation in the erythrocytes of rats fed a high-cholesterol diet. Sprague-Dawley male rats weighing 100 10 g were randomly assigned to nine experimental groups and fed 0.5 % cholesterol. The HC group did not receive any supplement, while the MP group was supplemented with 0.1 % methanol extract of PL seed, the MP2 group with 0.2 % methanol extract of PL seed, the EP1 group with 0.05 % ether-soluble fraction of PL seed, the EP2 group with 0.1 % ether-soluble fraction of PL seed, the MS1 group with 0.05 % methanol extract of FVL, the MS2 group with 0.1 % methanol extract of FVL, the ES group with 0.025% ethyl acetate-soluble fraction of FVL, and the ES2 group with 0.05 % ethyl acetate-soluble fraction of FVL. The experimental diets were fed ad libitum for 3 weeks. The erythrocyte SOD activity in the EP1 and EP2 groups increased 38% and 59%, respectively, when compared with the HC group, while the erythrocyte GSHpx activity in the EP1, EP2, and ES2 groups increased 30%, 31 %, and 29%, respectively, when compared with the HC group. The level of erythrocyte TBARS was significantly lower in the MP2, EP1, and EP2 groups than in the HC group, yet the level of serum TBARS was significantly lower in the all supplemented groups than in the HC group. The level of serum HDL- TBARS was significantly lower in the EP1 and EP2 groups than in the HC group, while the level of serum LDL- TBARS was significantly lower in the all the supplemented groups than in the HC group. Accordingly, the results indicated that the PL seed extracts and FVL extracts reduced oxidative damage by activating the antioxidative defense system in the erythrocytes of rats fed a high-cholesterol diet.

• 한국식품저장유통학회지
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• 제21권2호
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• pp.254-263
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• 2014

본 연구에서는 백합과에 속하는 덩굴성 관목인 청미래덩굴 70% 에탄올 추출물과 열수 추출물의 항산화 및 항당뇨에 대한 활성을 연구하였다. 총 폴리페놀 함량 과 플라보노이드 함량 측정, 전자공여능, ABTs 소거능, SOD 유사활성능, xanthine oxidase 저해활성, ACE 저해활성, TBARs 측정, PF값 측정 및 ${\alpha}$-glucosidase 와 ${\alpha}$-amylase 의 저해활성을 측정하였다. 그 결과 총 폴리페놀 함량과 플라보노이드 함량은 에탄올 추출물에 함유량이 더 많았으며, 전자공여능, ABTs 소거능, SOD 유사활성능, xanthine oxidase 저해활성, ACE 저해활성, TBARs 측정, PF값 측정 결과 모두 청미래 덩굴 열수 추출물보다 에탄올 추출물의 활성이 우수하였다. 또한 ${\alpha}$-glucosidase 와 ${\alpha}$-amylase 의 저해활성을 측정한 결과 에탄올 추출물의 활성이 열수 추출물보다 우수하였다. 이상의 검증 결과를 종합하여 볼 때 청미래 덩굴의 에탄올 추출물의 항산화 및 항 당뇨 활성이 우수하였고, 차후 항산화 및 항 당뇨 예방물질 소재로서의 활용이 가능할 것으로 판단된다.

• 대한지역사회영양학회지
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• 제7권4호
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• pp.527-538
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• 2002

Body antioxidant status is an important factor in the prevention of many chronic diseases caused by oxidative stress, especially in the elderly and is affected by health-related habits, such as smoking, drinking and regular physical activity. The aim of this study was to investigate the relationship between these health-related habits and plasma antioxidant status in the elderly. Plasma antioxidant status was examined by determining plasma levels of antioxidant vitamins (vitamin C, A, E, $\beta$ -carotene), total antioxidant status (TAS) and thiobarbituric acid-reactive substance (TBARS) . The subjects included 225 elderly persons aged over 60 yews (63 males, 162 females) living in the Ulsan area. They were interviewed to collect data on their general characteristics and health behaviors such as smoking, exercise and alcohol consumption by means of questionnaires. Their dietary intakes were obtained by means of semi-quantitative food frequency questionnaires (FFQ) The study population was divided into two or three groups according to their smoking, drinking, and exercise status. The ratio of smoker, drinker and exerciser was 16.7%, 31.0% and 44.2% respectively. The dietary antioxidant vitamin intakes were not significantly different among groups of smoking and drinking status, but tended to be higher in non-smokers and non-drinkers than in smokers and drinkers. Plasma vitamin C and $\beta$ -carotene levels were significantly higher in non-smokers, but Plasma vitamin A and TBARS levels were significantly lower in non-smokers than in smokers. Plasma TAS was not significantly different among the smoking groups, but showed a tendency to decrease with an increase in the number of packyear. Plasma vitamin C and $\beta$ -carotene levels of the non-drinkers were higher than those of drinkers and past-drinkers, but plasma vitamin A, C, E, TAS and TBARS showed no difference among the groups of drinker. All vitamin intakes of the exercisers were slightly higher than those of the non-exercisers, but vitamin C intake was significantly higher in female exercisers than in non-exercisers. Plasma $\beta$ -carotene levels were significantly higher in male exercisers and plasma vitamin A, C, E, TAS and TBARS levels tended to be higher in exercisers than in non-exercisers. These results suggested that change to non-smoker, modulation of alcohol consumption and regular exercise could enhance antioxidant defences against reactive oxygen species and might increase the likelihood of a healthier life span.

• 한방재활의학과학회지
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• 제21권1호
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• pp.23-33
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• 2011

Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.

• 대한예방한의학회지
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• 제6권2호
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• pp.112-127
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• 2002

Objectives: Younnyeniksoobulrodan(延年益壽不老丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Younnyeniksoobulrodan(延年益壽不老丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase (GSH-px) in rat erythrocytes and liver. Methods: Sprague-Dawley rats divided into 4 gorups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging gorup(injected D-galatose 50mg／kg, 1time／day for 6 weeks, CON) and Younnyeniksoobulrodan(延年益壽不老丹) administered group(D-galactose 50mg／kg and Younnyeniksoobulrodan extracts 840.0mg／kg 1time／day for 6 weeks, YIB). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were measured in rat erythrocytes and liver. Results: Plasma and liver TBARS concentrations of YIB group were significantly lower than those of control. Red blood cell(RBC) SOD activities of YIB group was increased(F=3.445, p=0.033, ANOVA test), and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of YIB group was increased(F=9.365,p=0.0001, ANOVA test). Liver SOD activities of YIB group was higher than those of control(F=4.967, p=0.008, ANOVA test). Liver catalase activities of all experimental group were not significantly different, and liver GSH-px activity of YIB group was significantly higher than that of control(F=3.846, p=0.022,ANOVA test). Conclusions: According to the above results, it is considered that Younnyeniksoobulrodan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.