• Journal of Advanced Marine Engineering and Technology
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• v.21 no.2
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• pp.195-205
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• 1997

The propulsion marine diesel engine have been widely applied with a mechanical- hydraulic governor to control the ship speed for long time. But it was recently very difficult for the mechani¬cal - hydraulic governor to control the speed of engine under the condition of low speed and low load because of jiggling by rough fluctuation of rotating torque and hunting by dead time of Desiel engnie The performance improvement of mechanical - hydraulic governor is required to solve these problems of control system. The electro - hydraulic governor using PID algorithm is provided to compensate the faults of mechanical- hydraulic governor. In this paper, in order to analyze the ship speed control system, the transfer function was converted from the z tansformation to w transformation. The influence of dead time changing by engine speed which induces hunting phenomena was investigated by Nichols chart of w plane. As a method of performance improvement of mechanical hydraulic governor, a Eletro - hydraulic governor shows that fine control results can be obtained through optimal parameter tuning of PID

• KSBB Journal
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• v.17 no.6
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• pp.582-585
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• 2002

In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.

• KSBB Journal
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• v.17 no.6
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• pp.577-581
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• 2002

In an attempt to produce recombinant proteins using the pollen enriched in some plant species, a 1.7 kb DNA encoding urease subunit B (UreB) amplified by PCR from Helicobacter pylori urease gene cluster in pH808 plasmid was cloned to be expressed under CaMV35S promoter in lily (Lilium longiflorum) pollen tubes elongated in vitro. Lily pollen at early germinating stage was transformed with the ureB DNA using Agrobacterium via vacuum infiltration and, incubated for a full pollen tube growth 16 - 24 h in the dark in the presence of kanamycin. DNA integration and expression in the transgenic pollen were analyzed by the standard molecular techniques and the results suggest that the pollen in vitro may be employed as a protein factory in a disposable fashion.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.12
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• pp.277-284
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• 2013

The analysis of phase synchronization characteristics from EEG signals is important for the understanding of information processing functionality in the brain network. In this paper, wavelet transformation(WT), Hilbert tansformation (HT), complex demodulation (CD) methods having time localization characteristics were applied to real evoked potential data and noise added simulation data with center frequencies corresponding to EEG bands for the estimation performance analysis of phase offset, phase changing point, and interband crosstalk. The WT is the best both in ${\delta}$, ${\theta}$, and ${\alpha}$ band signal decomposition, and in analyzing phase synchronization performance. The CD can be efficiently used in changing point detection under tolerant noise condition because of its abrupt performance degradation over noise endurance level. From experimental observations, the WT is the most suitable in phase synchronization application of EEG signal, and the CD can be affordable in restricted application such as changing point detection for higher bands than ${\delta}$. Particularly, WT and CD can be used to detect the changing instant of brain function by indirectly estimating the phase changing point.