• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.458-462
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• 2001

The localization of paclitaxel was investigated in suspension culture cells of Taxus chinensis. Over 93% of the cell-associated paclitaxel were detected throughout the entire culture period. Intracellular localization of paclitaxel over the culture time was analyzed further by cell fractionation for days 21 and 42. Paclitaxel contents in intracellular organelles were decreased at day 42, while the content in the cell wall fraction was increased at day 42 compared to the value for day 21. The localization of paclitaxel in the cell wall was confirmed by using the immunocytochemical method with the aid of a confocal laser scanning microscope.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.176-179
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• 2000

Nonpolar taxoides extracted from a large-scale cell culture of Taxus chinensis were isolated through the normal and reverse phase column chromatographies, and their compounds were identified via NMR spectroscopy. The complete separation method was systematically established and described. In dichloromethane, dissolved paclitaxel and other taxoids with hexane were precipitated during the purification of paclitaxel from the plant cell culture of T. chinensis through a large-scale process while the relatively nonpolar taxane derivatives remained dissolved in the hexane phase. 13-Deoxy baccatin III (I), baccatin VI (II), taxchinin I (III), $2{\alpha}$, $5{\alpha}$, $10{\beta}$, $14{\beta}$-tetraacetoxy-4(20), 11-taxadiene(IV), 1-deoxy baccatinVI(V), and taxayuntin C (VI) were isolated through column chromatography and identified via NMR spectroscopy. Compounds I and IV were found to the major components, aside from paclitaxel, in the plant cell culture of T. chinensis. The concentrations of I and IV were compared with the that concentration of the paclitaxel in each of plant cell culture. The possible applications of compounds I, II, IV, and V were discussed.

• Journal of Korean Society of Forest Science
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• v.100 no.1
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• pp.95-104
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• 2011

Distribution types of native conifers (Juniperus chinensis, Pinus parviflora, Tusga sieboldii and Taxus cuspidata var. latifolia) were studied by phytosociological investigation and ZM method in Ulleung Island, South Korea. Two main types were divided maritime vegetation (Juniperus chinensis forest) and mountain vegetation (Taxus cuspidata var. latifolia forest and Pinus parviflora-Tusga sieboldii forest). The former was divided into sea cliff distribution (J-SC) and sea ridge distribution (J-SR) type. The latter was classified 7 distribution types; Taxus cuspidata var. latifolia forest was rock distribution (Ta-R) and mountain slope distribution (Ta-MS) type, and Pinus parviflora-Tusga sieboldii forest was rock distribution (P T-R), upper and ridge distribution (P T-UR, 3 units sub-types:1sub, 2sub, 3sub), and Mountain slope distribution (P T-MS) type. It was considered that J-SC, Ta- R, and P T-R were maintained by topographic climax, but J-SR, Ta-MS, P T-UR and P T-MS were the process of vegetation succession. Distribution types of topographic climax are entrusted to process of vegetation succession. Types in the process of vegetation succession will be needed tending of forest to promote saplings growth and seedlings germination. Especially in order to restore Tsuga sieboldii forest should be afforest and make forest gap because It is mid shade tolerant tree and purity percentage of its seed is 1~2%. It was considered that the composition of group mixture forest constituted Pinus parviflora, Tsuga sieboldii, Taxus cuspidata, Camellia japonica, Machilus thunbergii and Acer okamotoanum, etc. will be able to restore native vegetation, after take the form of forest gap by strong thinning and pruning of Pinus thunbergii forest.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.164-171
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• 1994

To investigate the structure of natural forest at Nature Conservation Area in Tokyusan National Park, 29 plots (400$m^2$) set up with random sampling method. Four groups were classified by cluster analysis. High positive correlations was proved between Quernus mongolica and Fraxinus mandshurica, and Symplocos chinensis for. pilosa, and Lespedeza bicola, Acer tschonoskii var. rubripes and Taxus cuspidata, Magnolia sieboldii and Callicarpa japonica, Taxus cuspidata and Abies koreana, and high negative correlations was proved between Quercus mongolica and Acer tschonoskii var. rubripes, Taxus cuspidata, and Lonicera maackii. Species diversity(H') of investigated area was calculated 0.9969~1.2217.

• KSBB Journal
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• v.15 no.5
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• pp.537-540
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• 2000

In developing a HPLC purification process, it was hoped that a single chromatographic system would be sufficient to abtain pure paclitaxel in high yield. However, no such system was found, due in part to the complex taxoid profile of crude paclitaxel and to the rigorous nature of the product specification. A two step HPLC purification was adopted using reverse-phase separation on C(sub)18 as a first step, and normal-phase separation on silica as the final polishing step. Impurity profiles were established and maintained for paclitaxel, which identified and quantified each impurity observed in purified paclitaxel from these two steps, all impurities at or above 0.1% were identified. Results provide information for improving the quality control of paclitaxel production.

• The Korean Journal of Ecology
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• v.20 no.2
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• pp.125-132
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• 1997

A study of forest vegetation in Mt. $T\v{o}kyu$ National Park was investigated by ordination technique. By TWINSPAN(Two-Way Indicator Species Analysis) method, 10 groups were recognized as follows: pinus densiflora, Quercus variabilis, Quercus serrata, Quercus mongolica-Rhododendron schlippenbachii, Quercus mongolica-Abies koreana, Quercus mongolica-Acer pseudo-sieboldi-amum, Quercus mongolica-Symplocos chinensis for. pilosa, Carpinus laxiflora, Fraxinus mandshurica and Taxus cuspidata groups. The floristic composition of these groups showed high correlation to soil moisture(r=0.831), altitude(r=0.784), topography(r=-0.722), organic matter(r=0.642), and pH(r=-0.509) among various environmental factors. According to the results of CCA(Canonical Correspondence Analysis) Pinus densiflora group and Quercus variabilis group were situated in a xeric area at a lower altitude where soil nutrients were poor compared with the other groups. Fraxinus mandshurica group was distributed throughout the valley with high soil moisture and good nutrients, Quercus serrata group and Carpinus laxiflora group were found in the low altitude region with good nutrients, Quercus mongolica group, at the high altitude region with good nutrients, and Quercus mongolica-Acer koreana and Taxus cuspidata at higher altitudes(1, 400-1600 m).

• Journal of Ecology and Environment
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• v.43 no.4
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• pp.390-399
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• 2019

Background: The Northern Hemisphere forest ecosystem is a major sink for atmospheric carbon dioxide, and the subalpine zone stores large amounts of carbon; however, their magnitude and distribution of stored carbon are still unclear. Results: To clarify the carbon distribution and carbon budget in the subalpine zone at volcanic Jeju Island, Korea, we report the C stock and changes therein owing to vegetation form, litter production, forest floor, and soil, and soil respiration between 2014 and 2016, for three subalpine forest ecosystems, namely, Abies koreana forest, Taxus cuspidata forest, and Juniperus chinensis var. sargentii forest. Organic carbon distribution of vegetation and NPP were bigger in the A. koreana forest than in the other two forests. However, the amount of soil organic carbon distribution was the highest in the J. chinensis var. sargentii forest. Compared to the amount of organic carbon distribution (AOCD) of aboveground vegetation (57.15 t C ha^-1) on the subalpine-alpine forest in India, AOCD of vegetation in the subalpine forest in Mt. Halla was below 50%, but AOCD of soil in Mt. Halla was higher. We also compared our results of organic carbon budget in subalpine forest at volcanic island with data synthesized from subalpine forests in various countries. Conclusions: The subalpine forest is a carbon reservoir that stores a large amount of organic carbon in the forest soils and is expected to provide a high level of ecosystem services.

• Korean Journal of Environment and Ecology
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• v.11 no.1
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• pp.46-60
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• 1997

The purposes of this research are to select the appropriate kinds of landscape woody plants for afforestation artificial ground and to find out the way of developing planting-tree area of artificial ground through test planting and case study. The summary of the research is as follows; As a result of observing the state of trees' growth, trees on artificial grounds grow as well as, or even better than those on natural grounds. The kinds of trees which represent better growth states on artificial soil of rooftop test area are Thuja occidentalis, Prunus armericana var. ausu, Acer palmatum, Viburnum sargentii for. sterile, Buxus microphylla var. koreana, etc. In addition, soils than on natural soils. As a result of investigating the tree's growth state according to soil beds of artificial soils, shrubs don't represent distinct differences. They show that shrubs can be planted on 30cm soil bed. The rest of trees no differences according to the variation of soil beds, and they are in normal condition or have slight damage. This means that though arbor planting area of artificial ground is made 45~60cm, it is possible for trees to grow on it. In consequence of analyzing the cases on areas of landscaping artificial grounds, as the appropriate kinds of trees for afforestation artificial ground, Diospyros kaki, Hibiscus syriacus, Syringa dilatata, Magnolia kobus, Acer ginnala and cornus alba are selected in Capital region, and Nandina domestica, Taxus cuspidata, Ilex crenata, Viburnum awabuki, Aucuba japonica and Euonymus japonica are selected in southern region. In addition, Juniperus chinensis, Zelkova serrata, Juniperus chinensis var. kaizuka, etc. are selected regardless of regional character. In accordance with the state of soil it shows great difference, namely, fertilization once or more a year and irrigation for drought is advantageous to tree's growth. This research represents that it is necessary that standards related to landscaping artificial grounds are subdivided. In addition to it, the plan for revising related to regulation is drawn up.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.48-62
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• 2016

This study was conducted to classify forest vegetation structure and stand feature of Mt. Deogyusan National Park from Hyangjeukbong to Jungbong, 48 plots were surveyed. The type classification of the vegetation structure was performed with Z-M phytosociological method. As a result, Quercus mongolica community group was classified into the Picea jezoensis community, Carpinus cordata community and Tilia amurensis community in community unit. P. jezoensis community was subdivided into Deutzia glabrata group and Viburnum opulus var. calvescens group in group unit. D. glabrata group was subdivided into Acer mandshuricum subgroup and Ribes mandshuricum subgroup and V. opulus var. calvescens group was subdivided into Hemerocallis dumortieri subgroup and Prunus padus subgroup in subgroup unit. In the result of estimating the importance value, it constituted Q. mongolica (23.9%), Abies koreana (14.7%), Taxus cuspidata (10.2%), P. jezoensis (8.2%) and Betula ermanii (7.4%) in tree layer. It constituted Acer komarovii (18.6%), Acer pseudosieboldianum (18.4%) and Q. mongolica (8.9%) in subtree layer. It constituted Rhododendron schlippenbachii (20.7%), A. pseudosieboldianum (17.4%) and Symplocos chinensis (8.5%) in shrub layer. Indicator species analysis of vegetation unit 1 was consisted of Hydrangea serrata, Fraxinus mandshurica and D. glabrata that species prefer moist valley in subalpine or rocks. In the results of analyzing the species diversity, vegetation unit 1, 4 and 5 represented that there were different and complex local distributions. As in the similarity between the vegetation units, the vegetation units 1, 2, 3 and 4 represented high with 0.5 or above. It represented that there wasn't no differences on composition species in vegetation units.