• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1997.04a
• /
• pp.117-117
• /
• 1997

Sandimmune Neoral$\^$(R)/ and Neoplanta$\^$(R)/ capsules were administered to twenty four healthy Korean male subjects at a cyclosporin A (CsA) dose of 175 mg in a 2 ${\times}$ 2 crossover investigation with a two-week wash-out phase. Concentrations of CsA in blood were measured by RIA method for over a period of 48 h. Result : The two formulations were found bioequivalent, but analysis of variance (ANOVA) indicated that there is a significant (p<0.01) period effect in AUC$\_$0-LAST/ (area under the blood concentration above assay limit of quantification-time curve) and C$\_$MAX/ (maximum blood concentration) between the administrations. Paired t-test revealed 6 and 9% decreases in AUC$\_$0-LAST/ and C$\_$MAX/, respectively at the second administration. This period effect on the pharmacokinetics of CsA may be relevant for the patients who need consecutive administration of the drug. A number of mechanisms, such as induction of the enzymes responsible for metabolism of the drug in the gut wall and/or liver and modulation of P-glycoprotein upon the consecutive dosing, appear consistent with the change, and needs experimental proof.

• Journal of Microbiology and Biotechnology
• /
• v.8 no.1
• /
• pp.74-80
• /
• 1998

CS-2l lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA- 12 stromal cells isolated from lymph nodes. In order to obtain fundamental data on the identification and characterization of the soluble factors produced by CA-12 stromal cells, the conditioned medium of CA-12 stromal cells that inhibited apoptosis of CS-21 cells was examined. Various analytical treatments revealed that the soluble factors in CA-12 conditioned medium are very sensitive to heat treatment and trypsinization. Moreover CA-12 conditioned medium has an affinity with heparin but not with Con-A. In addition to these, the activity of CA-12 conditioned medium was blocked by H-7, a PKC inhibitor, but the conditioned medium could not induce the differentiation of thymocytes. We concluded that CA-12 conditioned medium contains stromal cell-derived apoptosis-inhibitory molecules that play an important role in proliferation of CS-2l cells by suppressing cell apoptosis.

• Journal of Korean Medical classics
• /
• v.32 no.3
• /
• pp.47-57
• /
• 2019

Objectives : We aim to assist in choosing an appropriate method for word extraction when analyzing East Asian Traditional Medical texts based on unsupervised learning. Methods : In order to assign ranks to substrings, we conducted a test using one method(BE:Branching Entropy) for exterior boundary value, three methods(CS:cohesion score, TS:t-score, SL:simple-ll) for interior boundary value, and six methods(BExSL, BExTS, BExCS, CSxTS, CSxSL, TSxSL) from combining them. Results : When Miss Rate(MR) was used as the criterion, the error was minimal when the TS and SL were used together, while the error was maximum when CS was used alone. When number of segmented texts was applied as weight value, the results were the best in the case of SL, and the worst in the case of BE alone. Conclusions : Unsupervised-Learning-Based Word Extraction is a method that can be used to analyze texts without a prepared set of vocabulary data. When using this method, SL or the combination of SL and TS could be considered primarily.

• Food Science of Animal Resources
• /
• v.43 no.1
• /
• pp.157-169
• /
• 2023

Effects of culture supernatants of Lactobacillus reuteri MG5346 (CS-MG5346) on receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis were examined. CS-MG5346 treatment up to 400 ㎍/mL significantly reduced tartrate-resistant acid-phosphatase (TRAP) activity, the phenotype biomarker of osteoclast, without affecting cell viability. CS-MG5346 inhibited the expression of osteoclast specific transcriptional factors (c-fos and nuclear factor-activated T cells c1) and their target genes (TRAP, cathepsin, and matrix metallo-proteinase-9) in a dose-dependent manner (p<0.05). The administration of L. reuteri MG5346 (2×10⁸ CFU/day) for 8 wks significantly improved furcation involvement, but no difference was observed in alveolar bone loss in ligature-induced experimental periodontitis rats. The elevated RANKL/osteoprotegerin ratio, the biomarker of periodontitis, was significantly lowered in the gingival tissue by administration of L. reuteri MG5346 (p<0.05). L. reuteri MG5346 showed excellent stability in simulated stomach and intestinal fluids and did not have antibiotic resistance. Based on the results, L. reuteri MG5346 has the potential to be a promising probiotic strain for oral health.

• Journal of Korea Society of Digital Industry and Information Management
• /
• v.17 no.4
• /
• pp.43-52
• /
• 2021

IoT applications are quickly spread in many fields. Blockchain methods(BC), defined as a distributed sharing mechanism, offer excellent support for IoT evolution. The BC provides a secure way for communication between IoT devices. However, the IoT environments are threatened by hacker attacks and malicious intrusions. The IoT applications security are faced with three challenges: intrusions and attacks detection, secure communication, and compressed storage information. This paper proposed a system based on double-blockchain to improve the communication transactions' safety and enhance the information compression method for the stored data. Information security is enhanced by using an Ellipse Curve Cryptography(ECC) considered in a double-blockchain case. The data compression is ensured by the Compressed Sensing(CS) method. The conducted experimentation reveals that the proposed method is more accurate in security and storage performance than previous related works.

• Journal of Acupuncture Research
• /
• v.32 no.3
• /
• pp.69-81
• /
• 2015

Purpose : The first purpose of this study is to find out whether the water extract of Rehmanniae Radix Preparat(RRP), Cuscutae Semen(CS) and their combination(Ssangbohwan, SBH) have the effect of suppressing Receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation. The second purpose of this study is to find out whether the water extract of RRP, CS and SBH have the effect of inhibiting osteoporosis in an osteoporosis model induced by lipopolysaccharide(LPS). Methods : After promoting differentiation of osteoclasts by treating the RANKL, we observed the effect by the administration of RRP, CS and SBH. In addition, by means of Reverse transcription polymerase chain reaction(RT-PCR), we assayed mRNA expression levels of NFATc1, c-Fos, TRAP and GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, spermatogeni) from bone marrow macrophages(BMMs). Similarly, the protein expression levels of NFATc1 (Nuclear factor of activated T-cells, cytoplasmic1), C-Fos, MAPKs(Mitogen-activated protein kinases) and ${\beta}$-actin in cell lysates were analyzed by means of Western Blotting. Finally, we determined the anti-osteoporotic effects of RRP, CS and SBH, through the use of Lipopolysaccharide-induced bone-loss mouse. Results : RRP, CS and SBH showed remarkable inhibitive effect on RANKL-treated osteoclast differentiation without cytotoxicity. SBH inhibited the phosphorylation of p38, Jun N-terminal kinases(JNK), and I-${\kappa}B$ and down-regulated the induction of c-Fos and NFATc1 by RANKL. RRP, CS suppressed degradation of I-${\kappa}B$, but it did not affect c-Fos and NFATc1 by RANKL. Lastly, in vivo data showed that RRP and SBH prevented bone erosion by LPS treatment. Conclusions : These results demonstrate SBH can be effective remedy for bone-loss diseases such as osteoporosis.

• The Research Journal of the Costume Culture
• /
• v.22 no.4
• /
• pp.555-564
• /
• 2014

This study aims to develop motifs and patterns for fashion cultural product designs in order to promote the Iksan Seodong Festival, which is the most representative festival in Iksan region. The motifs and patterns developed in this study can be used to introduce the distinct traditional culture depicting the legendary Seodong tale. This study used Adobe Illustrator CS4 and Adobe Photoshop CS4 to reconstruct the lotus motifs found from the green-oiled-lotus-motif rafter tile, the atrifact from Iksan Mireuksaji Museum. This lotus motif and the motif based on the Iksan City's logo were applied to a repeating pattern with colorways using the CMYK found from the Iksan city's logo. A total of 24 product designs, including 8 necktie designs, 8 scarf designs, and 8 T-shirts designs, were presented for fashion cultural products. The designs developed in this study can promote not only the local Iksan culture but also the national image of Korea on a global scale.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2022.10a
• /
• pp.514-516
• /
• 2022

Busan Metropolitan City runs Community Health Center which aims to health care service based on the community, besides the established health medical service towards people with low-income. 71 centers are run in 2021, therefore, this papear explores the way of digital transition of these centers. Digital Technologies possibly adopted inside the centers are: First, smart devices to advance the management system of chronical diseases based on health index; Second, IoT with mobile tech to manage health index; Third, AI Robot to care lone elderly people and to warn the emergency.

• Plant Biotechnology Reports
• /
• v.5 no.2
• /
• pp.157-167
• /
• 2011

In order to establish a reliable and highly efficient method for genetic transformation of pepper, a monitoring system featuring GFP (green fluorescent protein) as a report marker was applied to Agrobacteriummediated transformation. A callus-induced transformation (CIT) system was used to transform the GFP gene. GFP expression was observed in all tissues of $T_0$, $T_1$ and $T_2$ peppers, constituting the first instance in which the whole pepper plant has exhibited GFP fluorescence. A total of 38 T0 peppers were obtained from 4,200 explants. The transformation rate ranged from 0.47 to 1.83% depending on the genotype, which was higher than that obtained by CIT without the GFP monitoring system. This technique could enhance selection power by monitoring GFP expression at the early stage of callus in vitro. The detection of GFP expression in the callus led to successful identification of the shoot that contained the transgene. Thus, this technique saved lots of time and money for conducting the genetic transformation process of pepper. In addition, a co-transformation technique was applied to the target transgene, CaCS (encoding capsaicinoid synthetase of Capsicum) along with GFP. Paprika varieties were transformed by the CaCS::GFP construct, and GFP expression in callus tissues of paprika was monitored to select the right transformant.

• Journal of Radiation Protection and Research
• /
• v.8 no.1
• /
• pp.26-32
• /
• 1983

A somewhat detailed energy spectra in terms of the track length resulting from coupled electron-photon slowing down are calculated throughout the ICRU standard tissue with uniformly distributed gamma sources of $^{60}Co\;and\;^{137}Cs$, respectively. The calculation was accomplished by utilizing the latest available cross-section data as input to a carefully optimized computer code. In this report, the calculational method is described in detail. Furthermore, results of calculations are given in graphical form. The results show that the energy spectrum defined in terms of differential track length has about same shape although the energies of gamma source are different. The discontinuity in the energy spectrum appears at the energy of $T=(1/T_0+2/m_0c^2)^{-1}$, because a primary photon can not be degrade to a point below this energy.